Effects of Low-Level Laser Therapy, 660 nm, in Experimental Septic Arthritis

The effectiveness of low-level laser therapy (LLLT) in the presence of an infectious process has not been well elucidated. The aim of the study was to evaluate the effects of LLLT in an experimental model of septic arthritis. Methods. Twenty-one Wistar rats were divided as follows: control group, no bacteria; placebo group, bacteria were inoculated; Treated group, bacteria were injected and treatment with LLLTwas performed. To assess nociception, a von Frey digital analgesimeter was applied. Synovial fluid was streaked to analyze bacterial growth. The standard strain of S. aureus was inoculated in the right knee. LLLT was performed with 660 nm, 2 J/cm2, over 10 days. After treatment, the knees were fixed and processed for morphological analysis by light microscopy. Results. It was found that nociception increases in the right knee. There was a lack of results for the seeding of the synovial fluid. The morphological analysis showed slight recovery areas in the articular cartilage and synovia; however, there was the maintenance of the inflammatory infiltrate. Conclusion. The parameters used were not effective in the nociception reduction, even with the slight tissue recovery due to the maintenance of inflammatory infiltrate, but produced no change in the natural history of resolution of the infectious process.

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Low-level laser therapy, at 830 nm, for pain reduction in experimental model of rats with sciatica

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2011000300017 ◽

2011 ◽

Vol 69 (2b) ◽

pp. 356-359 ◽

Cited By ~ 20

Author(s):

G R F Bertolini ◽

E L Artifon ◽

T S Silva ◽

D M Cunha ◽

P R Vigo

Keyword(s):

Laser Therapy ◽

Pain Reduction ◽

Low Level Laser Therapy ◽

Painful Condition ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Before And After ◽

Common Clinical Presentation ◽

The Right

Chronic pain, resulting from nerve compression, is a common clinical presentation. One means of conservative treatment is low-level laser therapy, although controversial. The aim of this study was to evaluate the effects of two doses of low-level laser, at 830 nm, on pain reduction in animals subjected to sciatica. Eighteen rats were used, divided into three groups: GS (n=6), sciatica and simulated treatment; G4J (n=6), sciatica and treatment with 4 J/cm²; and G8J (n=6), sciatica and irradiation with 8 J/cm². The right sciatic nerve was exposed and compressed using catgut thread. Five days of treatment were started on the third postoperative day. Pain was assessed by means of the paw elevation time during gait: before sciatica, before and after the first and second therapies, and the end of the fifth therapy. Low-level laser was effective in reducing the painful condition.

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Low-level laser on femoral growth plate in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502012000200004 ◽

2012 ◽

Vol 27 (2) ◽

pp. 117-122 ◽

Cited By ~ 6

Author(s):

Sidney Piesco de Oliveira ◽

Sheila Canevese Rahal ◽

Elenize Jamas Pereira ◽

Paulo Ricardo de Oliveira Bersano ◽

Fabio de Almeida Vieira ◽

...

Keyword(s):

Laser Therapy ◽

Growth Plate ◽

Low Level Laser Therapy ◽

Low Level ◽

Low Level Laser ◽

Calcified Cartilage ◽

Level Laser ◽

Longitudinal Length ◽

Male Wistar Rats ◽

The Right

PURPOSE: To determine the influence of low-level laser therapy on femoral growth plate in rats. METHODS: Thirty male Wistar rats aged 40 days were divided into two groups, G1 and G2. In G1 the area of the distal growth plate of the right femur was irradiated at one point using GaAlAs laser 830 nm wavelength, output power of 40 mW, at an energy density of 10 J/cm². The irradiation was performed daily for a maximum of 21 days. The same procedure was done in G2, but the probe was turned off. Five animals in each group were euthanized on days 7, 14 and 21 and submitted to histomorphometric analysis. RESULTS: In both groups the growth plate was radiographically visible at all moments from both craniocaudal and mediolateral views. On the 21st day percentage of femoral longitudinal length was higher in G2 than G1 compared to basal value while hypertrophic zone chondrocyte numbers were higher in G1 than G2. Calcified cartilage zone was greater in G1 than in G2 at all evaluation moments. Angiogenesis was higher in G1 than in G2 at 14th and 21st days. CONCLUSION: The low-level laser therapy negatively influenced the distal femoral growth plate.

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Low-level laser therapy (LLLT) reduces inflammatory infiltrate and enhances skeletal muscle repair: Histomorphometric parameters

Laser Physics ◽

10.1134/s1054660x12090113 ◽

2012 ◽

Vol 22 (9) ◽

pp. 1425-1430 ◽

Cited By ~ 7

Author(s):

E. L. Paiva-Oliveira ◽

N. C. Lima ◽

P. H. Silva ◽

N. T. A. Sousa ◽

F. S. Barbosa ◽

...

Keyword(s):

Skeletal Muscle ◽

Laser Therapy ◽

Inflammatory Infiltrate ◽

Low Level Laser Therapy ◽

Muscle Repair ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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Evaluation of the Effect of Low Level Laser Therapy and Synovial Fluid-Mesenchymal Stem Cells in Cases of Temporomandibular Joint Disorders

Egyptian Journal of Oral and Maxillofacial Surgery ◽

10.21608/omx.2021.51515.1101 ◽

2020 ◽

Vol 11 (3) ◽

pp. 103-110

Author(s):

Mosaad Khalifah

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Synovial Fluid ◽

Temporomandibular Joint ◽

Laser Therapy ◽

Temporomandibular Joint Disorders ◽

Low Level Laser Therapy ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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Evaluation of Aquaporins 1 and 5 Expression in Rat Parotid Glands After Volumetric Modulated Arc Radiotherapy and Use of Low-Level Laser Therapy at Different Times

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.44 ◽

2020 ◽

Vol 11 (3) ◽

pp. 262-267

Author(s):

Gabriel Francisco Krueger ◽

Milene Castilhos de Oliveira ◽

Humberto Thomazi Gassen ◽

Juliana Tomaz Sganzerla ◽

Daniel Simon ◽

...

Keyword(s):

Gene Expression ◽

Parotid Gland ◽

Laser Therapy ◽

Low Level Laser Therapy ◽

Parotid Glands ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

The Right ◽

Experimental Group

Introduction: This experimental study investigated the mRNA expression of aquaporins (AQPs) 1 and 5 in the parotid glands of rats irradiated with volumetric modulated arc therapy (VMAT) and subjected to low-level laser therapy (LLLT) at different time points. Methods: The sample consisted of 30 Wistar rats (Rattus norvegicus) divided into the following groups: control, LLLT alone (LG), radiotherapy alone (RG), and experimental groups that received LLLT at 24 hours (early experimental group [EEG], n=12) and 120 hours (late experimental group [LEG], n=12) after radiotherapy. VMAT was delivered at a single dose (12 Gy) and LLLT was performed with an aluminium-gallium-arsenide diode laser (660 nm, 100 mW), spot area of 0.0028 cm2, energy of 2 J/cm2 applied to 3 spots in the region corresponding to the right parotid gland, for 10 consecutive days. The right parotid gland was resected and prepared for RNA extraction. The gene expression of AQPs was evaluated by quantitative polymerase chain reaction (qPCR) using specific TaqMan probes, with the HPRT gene as an internal control. Results: The lowest AQP1 gene expression was 0.83 (0.27) with the use of LLLT 24 hours after radiotherapy (EEG), and the highest was 1.56 (0.80) with the use of LLLT alone (LG). Likewise, the lowest AQP5 gene expression was found in the EEG (mean = 0.88; SD = 0.49) and the highest in the LG (mean = 1.29; SD = 0.33). Conclusion: The use of LLLT after radiotherapy may contribute to the maintenance and an increase of these proteins, even when used at a later time point after radiotherapy.

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Low-Level Laser Therapy Approach of Bilateral Necrotizing Sialometaplasia of the Hard and Soft Palates

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.56 ◽

2020 ◽

Vol 11 (3) ◽

pp. 348-354

Author(s):

Milena Gomes Melo Leite ◽

Eliandro de Souza Freitas ◽

Antonio Jorge Araújo de Vasconcelos II ◽

Tiago Novaes Pinheiro

Keyword(s):

Laser Therapy ◽

Treatment Protocol ◽

Low Level Laser Therapy ◽

Clinical Report ◽

Posterior Portion ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Necrotizing Sialometaplasia ◽

The Right

Introduction: Necrotizing sialometaplasia (NS) is a rare locally destructive inflammatory benign disease that commonly affects the minor salivary glands. It is frequently associated with the glands located in the posterior portion of the hard and soft palates. Low-level laser therapy (LLLT), also called photobiomodulation therapy (PBMT), has been deemed a substantial method for the regenerative wound process. Case Presentation: A 32-year-old male patient was referred with a chief complaint of two asymptomatic crater-like ulcers measuring approximately 1.5 cm wide on the right side of the hard and soft palates, and another measuring 0.3 cm wide on the left side of the hard palate. The lesion had two weeks of evolution followed by a previous infectious "sore throat" event that kept the patient hospitalized for 4 days. A clinical diagnosis of NS was made. LLLT was applied during 2 sessions per week, favoring the total wound healing within 2 weeks. At 3 months of clinical followup, the patient did not present any complication or relapse and was thus released. Conclusion: This is, to our knowledge, the first clinical report of LLLT applied for the management of NS. Large palatal ulcers caused by NS usually have long healing periods. The shortened healing period observed in this case encourages the inclusion of LLLT in any treatment protocol for similar lesions.

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The Effects of Low-Level Laser Therapy, 670 nm, on Epiphyseal Growth in Rats

The Scientific World JOURNAL ◽

10.1100/2012/231723 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Adriana Regina de Andrade ◽

Anamaria Meireles ◽

Elisangela Lourdes Artifon ◽

Rose Meire Costa Brancalhão ◽

José Roberto Leonel Ferreira ◽

...

Keyword(s):

Laser Therapy ◽

Histological Analysis ◽

Low Level Laser Therapy ◽

Total Thickness ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Final Length ◽

The Right ◽

Different Doses

The longitudinal growth of long bones is attributed to epiphyseal growth. However, the effects of low-level laser therapy (LLLT) in such structures has still not been studied extensively in the literature. Therefore, the aim of this study was to evaluate the use of LLLT, 670 nm, at three different doses on the epiphyseal growth of the right tibia of rats. Twenty-one Wistar rats, aged four weeks, were subjected to the application of LLLT, with dosage according to the group (G4: were submitted to the application of 4 J/cm2; G8: were submitted to the application of 8 J/cm2; G16: were submitted to the application of 16 J/cm2). After completion of protocol they were kept until they were 14 weeks of age and then submitted to a radiological examination (evaluation of limb length) and euthanised. The histological analysis of the growth plates (total thickness and hypertrophic and proliferative zones) was then performed. Comparisons were made with the untreated left tibia. No differences were observed in any of the reviews (radiological and histological), when comparing the right sides (treated) to the left (untreated). It was concluded that the treatment with LLLT within the parameters used caused changes neither in areas of the epiphyseal cartilage nor in the final length of limbs.

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Healing of Skin Wounds in Rats: Influence of Low-Level Laser Therapy, Alone or Combined with Natural Products

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.99845 ◽

2020 ◽

Vol 48 ◽

Author(s):

Diana Milanez Ávila Dias Maciel ◽

Sheila Canevese Rahal ◽

Carlos Eduardo Fonseca Alves ◽

Ivan Felismino Charas dos Santos ◽

Alexandre Botelho De Abreu Sampaio ◽

...

Keyword(s):

Wound Healing ◽

Natural Products ◽

Laser Therapy ◽

Inflammatory Infiltrate ◽

Low Level Laser Therapy ◽

Skin Wounds ◽

Wound Area ◽

Low Level ◽

Low Level Laser ◽

Level Laser

Background: A great variety of natural products have been evaluated for the skin wound healing due to anti-inflammatory, antioxidant and antibacterial activities, and procollagen synthesis, of which may be mentioned the honey and propolis. In turn, low-level laser therapy (LLLT) is considered an important tool in the treatment of skin wounds, because of ability to raise the ATP production, to stimulate the microcirculation, and formation of new blood vessels. Therefore,this study aimed to assess the influence of LLLT, alone or combined with natural products, in the healing of excisional skin wounds in rats. Materials, Methods & Results: One hundred twenty-six male Wistar rats, aged 14 weeks, were randomly divided into seven groups (n = 18 per group) according to wound treatments: G1 (negative control): 0.9% saline solution; G2 (positive control): allantoin + zinc oxide ointment; G3: LLLT; G4: mixture of honey + 5% propolis hydroalcoholic solution; G5: LLLT + honey + hydroalcoholic solution of 5% propolis; G6: LLLT + honey in natura; G7: LLLT + 5% propolis hydroalcoholic solution. Six rats from each group were euthanized on the 7th, 14th, and 21th postoperative days. Macroscopic and histological evaluations of wound healing were performed. A decrease in wound area occurred in all groups, but in general G4 had the higher reduction and G1 and G3 had the lower reductions. These findings corresponded to the percentage of wound contraction, since the largest contraction was G4 and the lowest contractions were G1 and G3. Histological analysis showed no significant difference among groups on the 7th postoperative day. On postoperative day 14, a significant decrease in hemorrhage occurred between G1xG4, G1xG5 and G1xG7, and a significant decrease in congestion between G1xG7 and G2xG5. In addition, intensity of the inflammatory infiltrate showed a significant reduction between G1xG7, G2xG7 and G3xG7. On postoperative day 21, a significant changed from mixed to mononuclear inflammatory infiltrate was observed between G1xG3, G1xG4, G1xG6, G1xG7, G2xG3, and G2xG6. Regarding organization of fibroblasts, there were differences between G1xG6 and G1xG7. Discussion: Spite the red laser has been associated to in increase fibroblast proliferation, G3 showed inadequate decrease in wound area and percentage of contraction. Histological examination showed no differences among groups in the inflammatory phase. However, in the proliferative and remodeling phases around days 14 and 21 were found some differences among groups, which may be related to the actions of the natural products, or laser. Studies have shown that honey acts as a repair and anti-inflammatory agent in skin woundthat may be associated to lower hemorrhage in G4 and G4 compared to G1 on day 14. Propolis is related to reduction of free radicals and increasing the amount of collagen, which may have contributed for quality improvement of the inflammatory infiltrate in G4 and G7 compared to G1, and fibroblast organization in G7 at postoperative day 21. In conclusion, macroscopically the mixture of honey + 5% propolis hydroalcoholic solution was the most effective in reducing wound area and increasing wound contraction. However, based on the parameters evaluated histologically, may be highlighted the evolution of the treatments with LLLT + honey, as well as LLLT + 5% propolis hydroalcoholic solution. Keywords: honey, propolis, biomodulation, wound, healing.

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Assessment of healing of dental implant surgical site following Low-Level Laser Therapy using Bio-Clinical parameters: An Exploratory Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-18-00316 ◽

2020 ◽

Author(s):

VARSHA PALLED ◽

DR. JITENDRA RAO ◽

DR. RAGHUWAR DAYAL SINGH ◽

DR. SHUCHI TRIPATHI ◽

DR. KALPANA SINGH ◽

...

Keyword(s):

Laser Therapy ◽

Test Group ◽

Low Level Laser Therapy ◽

Control Group ◽

Clinical Parameters ◽

Implant Stability ◽

Low Level ◽

Implant Stability Quotient ◽

Low Level Laser ◽

Level Laser

The purpose of this study was to evaluate whether Low-Level Laser Therapy (LLLT) improves the healing of the implant surgical site with clinical and biochemical parameters.Thirty patients with an edentulous space spanning a single tooth were selected. The patients were randomly allocated to two groups - the control group and the test group. The test group received laser energy at a power of 2J/cm 2 with a total of 4-6J energy over each implant. Clinical parameters (Implant Stability Quotient, probing index, modified sulcus bleeding index)and osteoprotegerin (OPG) were assessed at baseline and follow-up intervals (2 weeks, 6 weeks, and 3 months).The test group showed significantly higher implant stability quotient compared to thecontrol group at 2 weeks(57.93±3.95 and 35.67±3.08; p<0.01) and 3months(58.86±3.75 and 67.06±3.78; p<0.01). A significant rise in OPG levels of the test group(686.30±125.36pg/ml at baseline and 784.25±108.30pg/ml at 3months;p<0.01) was seen contrary to significant decline in the control group (839.50±249.08pg/ml at baseline,415.30±78.39pg/ml at 3months;p<0.01). Within the limitations of the study, the study suggests that the healing of peri-implant hard and soft tissues may be enhanced with the use of LLLT as an explicit modality during the post-operative period.

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