Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice: Analysis with Differential Acridine Orange/Ethidium Bromide Staining

This study was conducted to investigate the modulating effects of green tea polyphenols on genotoxic damage and apoptotic activity induced by hexavalent chromium [Cr (VI)] in CD-1 mice. Animals were divided into the following groups: (i) injected with vehicle; (ii) treated with green tea polyphenols (30 mg/kg) via gavage; (iii) injected with CrO3(20 mg/kg) intraperitoneally; (iv) treated with green tea polyphenols in addition to CrO3. Genotoxic damage was evaluated by examining micronucleated polychromatic erythrocytes (MN-PCEs) obtained from peripheral blood at 0, 24, 48, and 72 h after treatment. Induction of apoptosis and cell viability were assessed by differential acridine orange/ethidium bromide (AO/EB) staining. Treatment of green tea polyphenols led to no significant changes in the MN-PCEs. However, CrO3treatment significantly increased MN-PCEs at 24 and 48 h after injection. Green tea polyphenols treatment prior to CrO3injection led to a decrease in MN-PCEs compared to the group treated with CrO3only. The average of apoptotic cells was increased at 48 h after treatment compared to control mice, suggesting that apoptosis could contribute to eliminate the DNA damaged cells induced by Cr (VI). Our findings support the proposed protective effects of green tea polyphenols against the genotoxic damage induced by Cr (VI).

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The Role of Green Tea Polyphenols in the Protection from Hexavalent Chromium-Induced Genotoxic Damage

Polyphenols ◽

10.5772/intechopen.76651 ◽

2018 ◽

Cited By ~ 1

Author(s):

María del Carmen García-Rodríguez ◽

Mario Altamirano-Lozano ◽

Alejandro Gordillo-García

Keyword(s):

Green Tea ◽

Hexavalent Chromium ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Genotoxic Damage

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Modulation of hexavalent chromium-induced genotoxic damage in peripheral blood of mice by epigallocatechin-3-gallate (EGCG) and its relationship to the apoptotic activity

Journal of Toxicology and Environmental Health Part A ◽

10.1080/15287394.2015.1104525 ◽

2015 ◽

Vol 79 (1) ◽

pp. 28-38 ◽

Cited By ~ 5

Author(s):

María del Carmen García-Rodríguez ◽

Ana Rosa Montaño-Rodríguez ◽

Mario Agustín Altamirano-Lozano

Keyword(s):

Hexavalent Chromium ◽

Peripheral Blood ◽

Genotoxic Damage ◽

Apoptotic Activity

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Epigallocatechin Gallate (EGCG), a Green Tea Polyphenol, Reduces Coronavirus Replication in a Mouse Model

Viruses ◽

10.3390/v13122533 ◽

2021 ◽

Vol 13 (12) ◽

pp. 2533

Author(s):

Rackhyun Park ◽

Minsu Jang ◽

Yea-In Park ◽

Yeonjeong Park ◽

Woochul Jung ◽

...

Keyword(s):

Mouse Model ◽

Green Tea ◽

Particle Production ◽

Epigallocatechin Gallate ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Tea Polyphenol ◽

Green Tea Polyphenol

The COVID-19 pandemic has resulted in a huge number of deaths from 2020 to 2021; however, effective antiviral drugs against SARS-CoV-2 are currently under development. Recent studies have demonstrated that green tea polyphenols, particularly EGCG, inhibit coronavirus enzymes as well as coronavirus replication in vitro. Herein, we examined the inhibitory effect of green tea polyphenols on coronavirus replication in a mouse model. We used epigallocatechin gallate (EGCG) and green tea polyphenols containing more than 60% catechin (GTP60) and human coronavirus OC43 (HCoV-OC43) as a surrogate for SARS-CoV-2. Scanning electron microscopy analysis results showed that HCoV-OC43 infection resulted in virion particle production in infected cells. EGCG and GTP60 treatment reduced coronavirus protein and virus production in the cells. Finally, EGCG- and GTP60-fed mice exhibited reduced levels of coronavirus RNA in mouse lungs. These results demonstrate that green tea polyphenol treatment is effective in decreasing the level of coronavirus in vivo.

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Green Tea Polyphenols Induce Apoptosisin vitroin Peripheral Blood T Lymphocytes of Adult T-Cell Leukemia Patients

Japanese Journal of Cancer Research ◽

10.1111/j.1349-7006.2000.tb00857.x ◽

2000 ◽

Vol 91 (1) ◽

pp. 34-40 ◽

Cited By ~ 58

Author(s):

Hong-Chuan Li ◽

Shinji Yashiki ◽

Junichiro Sonoda ◽

Hong Lou ◽

Subrata K. Ghosh ◽

...

Keyword(s):

T Cell ◽

T Lymphocytes ◽

Green Tea ◽

Peripheral Blood ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

T Cell Leukemia ◽

Cell Leukemia ◽

Adult T Cell Leukemia

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Green Tea Polyphenols for the Protection against Renal Damage Caused by Oxidative Stress

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/845917 ◽

2012 ◽

Vol 2012 ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

Takako Yokozawa ◽

Jeong Sook Noh ◽

Chan Hum Park

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Green Tea ◽

Renal Damage ◽

Large Fraction ◽

Tea Polyphenols ◽

Green Tea Polyphenols ◽

Tea Polyphenol ◽

Green Tea Polyphenol ◽

Low Toxicity

Green tea, prepared from the leaves ofCamellia sinensisL., is a beverage that is popular worldwide. Polyphenols in green tea have been receiving much attention as potential compounds for the maintenance of human health due to their varied biological activity and low toxicity. In particular, the contribution of antioxidant activity to the prevention of diseases caused by oxidative stress has been focused upon. Therefore, in this study, we investigated the effects of (−)-epigallocatechin 3-O-gallate and (−)-epigallocatechin 3-O-gallate, which account for a large fraction of the components of green tea polyphenol, on oxidative stress-related renal disease. Our observations suggest that green tea polyphenols have a beneficial effect on pathological states related to oxidative stress of the kidney.

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