Serum Cytokines Correlated with The Disease Severity of Generalized Pustular Psoriasis

To characterize serum biomarkers reflecting the severity of generalized pustular psoriasis (GPP), we measured multiple cytokine/chemokine levels in 39 serum samples from 6 cases with GPP during the course of the disease. Serum levels of IL-4, IL-8, CXCL1 and CCL3 were positively correlated with the severity scores of GPP, white blood cell counts and serum C-reactive protein levels. Serum levels of IL-1β, IL-1ra, IL-6, IL-10, IL-12p70, IL-18, IL-22, IFN-γand VEGF showed strong positive correlations (r> 0.4,p< 0.01) with all those 3 clinical markers. Of those, IL-10 and IL-22 were significantly decreased after treatment in parallel with the GPP score and therefore those two serum cytokines might be useful to evaluate the efficacy of treatment for GPP.

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A Case of Old Age-Onset Generalized Pustular Psoriasis with a Deficiency of IL-36RN (DITRA) Treated by Granulocyte and Monocyte Apheresis

Case Reports in Dermatology ◽

10.1159/000380876 ◽

2015 ◽

Vol 7 (1) ◽

pp. 29-35 ◽

Cited By ~ 16

Author(s):

Chiharu Tominaga ◽

Masaaki Yamamoto ◽

Yasutomo Imai ◽

Kiyofumi Yamanishi

Keyword(s):

Receptor Antagonist ◽

Dose Reduction ◽

Old Age ◽

Psoriasis Vulgaris ◽

Serum Levels ◽

Pustular Psoriasis ◽

Generalized Pustular Psoriasis ◽

Homozygous Mutations ◽

Inflammatory Infiltrates

A 78-year-old woman who had been suffering from psoriasis vulgaris for 31 years was admitted to hospital because of her erythroderma. A toxic eruption was suspected and she was treated with prednisolone 30 mg daily. However, it was ineffective and, suspecting psoriatic erythroderma, cyclosporine 150 mg daily was administered with tapering of the prednisolone. Two weeks after a dose reduction of cyclosporine to 100 mg/day, erythroderma with widespread generalized pustules and fever developed. Histology of a biopsy revealed inflammatory infiltrates in the skin with a spongiform pustule of Kogoj, which was consistent with generalized pustular psoriasis (GPP). Her pustules improved with additional etretinate 20 mg/day, but the erythroderma persisted and she consulted us. Three sessions of granulocyte and monocyte apheresis once weekly were effective for her condition and decreased her serum levels of IL-6 and IL-8. She had homozygous mutations of c.[28C>T] in IL36RN which cause p.[Arg10Ter]. She is the oldest reported case of GPP with a deficiency of interleukin-36 receptor antagonist (DITRA), although GPP in DITRA has been suggested to usually occur in younger cases with no pre-existing psoriasis vulgaris.

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Association Between Vancomycin Blood Brain Barrier Penetration and Clinical Response in Postsurgical Meningitis

Journal of Pharmacy & Pharmaceutical Sciences ◽

10.18433/j3493f ◽

2017 ◽

Vol 20 ◽

pp. 161 ◽

Cited By ~ 1

Author(s):

Qing Wang ◽

Si Chen ◽

Yan-Gang Zhou ◽

Ping Xu ◽

Yi-Ping Liu ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Clinical Response ◽

Blood Brain Barrier ◽

Brain Barrier ◽

Serum Samples ◽

Cell Counts ◽

Blood Cell Counts ◽

Blood Brain ◽

Barrier Penetration ◽

White Blood Cell Counts

PURPOSE: This study investigated the association between vancomycin blood brain barrier penetration and clinical response in patients with postsurgical meningitis. METHODS: Adult patients with postsurgical meningitis were recruited. Eligible patients received vancomycin 500 mg every 6 h for at least 5 days. On day 3 or 4, cerebrospinal fluid (CSF) and simultaneous serum samples were obtained to determine CSF minimum concentrations (Cmin), serum Cmin and CSF to serum Cmin ratio. RESULTS: Twenty-two patients (14 men and 8 women; mean age of 52.6± 12.1 years) were recruited. The vancomycin Cmin was 3.63 ± 1.64 mg/L in CSF and 13.38 ± 5.36 mg/L in serum, with the CSF to serum Cmin ratio of 0.291 ± 0.118. The Cmin in serum and in CSF showed a significant correlation (p=0.005, r =0.575). The vancomycin CSF Cmin had a significant correlation with the decline of white blood cell counts (WBCs) in CSF (p=0.003, r =0.609). CSF Cmin, serum Cmin and CSF to serum Cmin ratio all showed no significant correlation with clinical response (p=0.335, 0.100, 0.679, respectively). CONCLUSIONS: There was a positive correlation between serum Cmin and CSF Cmin. However, only CSF Cmin is positively correlated with WBCs improvement in CSF. All other parameters such as serum Cmin, CSF Cmin and CSF to serum Cmin ratio had no correlation with clinical response. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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Increased Serum Levels of Growth-Related Oncogene-α in Patients with Generalized Pustular Psoriasis

Dermatology ◽

10.1159/000258674 ◽

2010 ◽

Vol 220 (1) ◽

pp. 46-48 ◽

Cited By ~ 2

Author(s):

Yasunobu Kato ◽

Toshiyuki Yamamoto

Keyword(s):

Serum Levels ◽

Pustular Psoriasis ◽

Generalized Pustular Psoriasis

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Clinical assessment of blood leukocytes, serum cytokines, and serum immunoglobulins as responses to sleep deprivation in laboratory rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00021.2005 ◽

2005 ◽

Vol 289 (4) ◽

pp. R1054-R1063 ◽

Cited By ~ 82

Author(s):

Carol A. Everson

Keyword(s):

Sleep Deprivation ◽

Mononuclear Phagocytes ◽

Blood Leukocytes ◽

Immune Effector ◽

Laboratory Rats ◽

Serum Cytokines ◽

Cytokines And Chemokines ◽

Cell Counts ◽

White Blood Cell Counts ◽

Differential White Blood Cell

The specific systems and mechanisms affected by sleep deprivation that may perpetuate disease processes in humans still are speculative. In laboratory rats, prolonged sleep deprivation induces a state marked by abnormal control over indigenous bacteria that results in transient infections of internal tissues and eventual lethal septicemia. The present studies investigated changes in blood, serum, and bone marrow parameters that may provide diagnostic clues to immunopathology. Prolonged sleep deprivation was produced in rats by the disk-over-water method, a well-established and selective means that does not interfere with normal waking behaviors. Measurements included bone and blood differential white blood cell counts, multiple serum cytokines and chemokines, several major Ig classes and subclasses, and serum endotoxin concentrations. The results indicated mild, regenerative neutrophilia in sleep-deprived rats, initially accompanied by immature neutrophils and later by monocytosis. The corresponding serum cytokine profile revealed an evolving proinflammatory state, particularly by high incidence of interleukin-1β, implicating mononuclear phagocytes and resident tissue cells as main intermediary sources. In addition, multiple serum Ig classes were increased by sleep deprivation without experimental administration of an exogenous antigen. Despite this immune activation, there was failure to eradicate invading bacteria and toxins, suggesting competing anti-inflammatory processes or interference with immune effector functions during sleep deprivation. Nearly all of the immune-related events that emerged as responses to sleep deprivation have been implicated as etiological or provocative factors in other disease processes and may provide means by which sleep deprivation as a risk factor in disease may become understood.

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Vancomycin-Induced Neutropenia during Treatment of Osteomyelitis in an Outpatient

Drug Intelligence & Clinical Pharmacy ◽

10.1177/106002808602001011 ◽

1986 ◽

Vol 20 (10) ◽

pp. 783-785 ◽

Cited By ~ 20

Author(s):

Keith Henry ◽

Irving Steinberg ◽

Kent B. Crossley

Keyword(s):

Serum Levels ◽

Pharmacokinetic Studies ◽

Clear Understanding ◽

Outpatient Therapy ◽

Vancomycin Therapy ◽

Cell Counts ◽

Blood Cell Counts ◽

Acceptable Range ◽

White Blood Cell Counts

A case of vancomycin-associated neutropenia occurring during long-term outpatient therapy with vancomycin is described. Pharmacokinetic studies demonstrated that the patient's vancomycin serum levels were within an acceptable range during treatment. Eighteen other reported cases of vancomycin-associated leukopenia are discussed in brief. An immunologic mechanism has been proposed but a clear understanding is lacking. Patients receiving long-term vancomycin therapy should have their white blood cell counts periodically monitored.

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Systemic Immune Profile in Patients With CRSwNP

Ear Nose & Throat Journal ◽

10.1177/0145561319893163 ◽

2019 ◽

pp. 014556131989316

Author(s):

Rafaela Veloso-Teles ◽

Rui Cerejeira ◽

Rosa Roque-Farinha ◽

Christian von Buchwald

Keyword(s):

Serum Levels ◽

Atopic Disease ◽

Differential Count ◽

Immunological Parameters ◽

Immune Profile ◽

Prick Test ◽

Cell Counts ◽

Significant Difference ◽

White Blood Cell Counts ◽

Igg1 Subclass

The immune pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) remains obscure. Our aim was to compare humoral immunity and white blood cell counts in patients with CRSwNP and controls. A prospective case–control study was carried out in 37 patients with CRSwNP and 34 controls without CRS. Clinical data were gathered through a systematic interview. Computed tomography scan, skin prick test, spirometry, and immunological parameters (leukocyte differential count, immunoglobulin classes, and immunoglobulin [Ig] G subclasses) in serum specimens were obtained. Statistical analysis was performed using SPSS v.23. The prevalence of chronic lower respiratory diseases was greater in the CRSwNP group ( P < .001), but atopic disease had no significant difference. A significantly higher eosinophil ( P < .001) and basophil relative count ( P = .022) and a lower relative neutrophil count ( P = .013) were found among CRSwNP group. Patients with CRSwNP had higher IgG1 ( P = .022), but lower IgG2 ( P = .014) and IgG3 ( P = .018) serum levels compared to controls; IgG4, total IgG, IgA, IgM, and IgE serum levels did not differ between groups, as well as the prevalence of immunoglobulin classes or IgG subclasses deficiency. The variation observed in peripheral relative leukocyte count and the systemic IgG1 subclass shift are similar to what is known to happen in nasal polyp tissue. A unique systemic immune profile seems to be present in patients with CRSwNP.

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Monokine Induced by Interferon-γ (MIG) Serum Levels Are a Marker of Disease Load and Correlate with Prognosis in Multiple Myeloma.

Blood ◽

10.1182/blood.v108.11.5040.5040 ◽

2006 ◽

Vol 108 (11) ◽

pp. 5040-5040

Author(s):

Martin Schreder ◽

Gudrun Koch ◽

Daniel Heintel ◽

Kathrin Strasser-Weippl ◽

Katrin Frischmuth ◽

...

Keyword(s):

Multiple Myeloma ◽

Overall Survival ◽

Prognostic Significance ◽

Serum Levels ◽

Interferon Γ ◽

Serum Samples ◽

Myeloma Cells ◽

Autocrine Loop ◽

Protein Levels

Abstract Background and Aims: Monokine-induced by interferon-γ (MIG) is a chemokine that is produced by monocytes and macrophages in response to interferon-γ and acts as a chemoattractant mainly to T-lymphocytes in inflammatory processes. MIG has also been suggested to act in an autocrine loop to stimulate tumour cells through its receptor CXCR3, which is known to be expressed in myeloma cells. However, it is presently unclear if MIG is of biologic significance in myeloma in vivo. We have recently shown that multiple myeloma oncogene 1 (MUM1) expression in myeloma cells correlates with prognosis in this disease (Heintel D et al., ASH 2005), and MUM1 was found to upregulate MIG gene expression in B cell malignancies (Uranishi M et al., Leukemia 2005). This led us to evaluate the potential prognostic significance of MIG serum levels in a series of well characterized myeloma patients. Methods: 105 newly diagnosed multiple myeloma patients (median age 69.3 years, range 39.4–90.5) were enrolled. 18 patients presented with Durie/Salmon stage I disease, 9 had stage II and 78 had stage III. MIG serum levels were determined by a commercially available ELISA (R&D Systems). Serum samples from 17 MGUS patients and 37 age-matched healthy volunteers were used as controls. Results: MIG serum levels were elevated in multiple myeloma patients (median 161.3 pg/ml, range 9.37–1966.0) compared to MGUS patients (median 92.7 pg/ml, range 6.29–1303.1) and healthy controls (median 106.2 pg/ml, range 51.0–390.6). For analysis of myeloma cases, a cut-off level for MIG of 200pg/ml (=95th percentile of MIG in controls) was chosen to identify low and high MIG expressers. Using the cut-off as defined above, 63 patients with low MIG serum levels and 42 high MIG expressers were identified in a population of 105 myeloma patients. MIG serum levels in myeloma patients showed strong correlations with several markers of tumour load including low albumin and high β2-microglobulin. Interestingly, no correlation was found with C-reactive protein levels, indicating that MIG is not associated with an inflammatory response in myeloma. Median survival was significantly shorter in patients with high MIG serum levels compared to patients with low MIG expression (median not reached vs. 17.0 months, p<0.001; see figure). Conclusions: MIG serum levels correlate with markers of disease burden in myeloma and high MIG levels are associated with a poor outcome in this disease. Overall Survival: low vs. high MIG serum levels Overall Survival: low vs. high MIG serum levels

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Differential IL-1 signaling induced by BMPR2 deficiency drives pulmonary vascular remodeling

Pulmonary Circulation ◽

10.1177/2045893217729096 ◽

2017 ◽

Vol 7 (4) ◽

pp. 768-776 ◽

Cited By ~ 7

Author(s):

Josephine Pickworth ◽

Alexander Rothman ◽

James Iremonger ◽

Helen Casbolt ◽

Kay Hopkinson ◽

...

Keyword(s):

Pulmonary Artery ◽

Vascular Remodeling ◽

Interleukin 1 ◽

Pulmonary Vascular Remodeling ◽

Protein Levels ◽

Cell Counts ◽

Protein Receptor ◽

White Blood Cell Counts ◽

Human Pulmonary Artery

Bone morphogenetic protein receptor type 2 (BMPR2) mutations are present in patients with heritable and idiopathic pulmonary arterial hypertension (PAH). Circulating levels of interleukin-1 (IL-1) are raised in patients and animal models. Whether interplay between BMP and IL-1 signaling can explain the local manifestation of PAH in the lung remains unclear. Cell culture, siRNA, and mRNA microarray analysis of RNA isolated from human pulmonary artery (PASMC) and aortic (AoSMC) smooth muscle cells were used. R899X+/– BMPR2 transgenic mice fed a Western diet for six weeks were given daily injections of IL-1ß prior to assessment for PAH and tissue collection. PASMC have reduced inflammatory activation in response to IL-1ß compared with AoSMCs; however, PASMC with reduced BMPR2 demonstrated an exaggerated response. Mice treated with IL-1ß had higher white blood cell counts and significantly raised serum protein levels of IL-6 and osteoprotegerin (OPG) plasma levels recapitulating in vitro data. Phenotypically, IL-1ß treated mice demonstrated increased pulmonary vascular remodeling. IL-1ß induces an exaggerated pulmonary artery specific transcriptomic inflammatory response when BMPR2 signaling is reduced.

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Serum Carbohydrate Antigen 199 as a Biomarker for Evaluating Patients with Choledocholithiasis

Gastroenterology Research and Practice ◽

10.1155/2020/2739612 ◽

2020 ◽

Vol 2020 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Wang Gu ◽

Zhong Tong

Keyword(s):

Correlation Analysis ◽

White Cell Count ◽

Elevated Serum ◽

Serum Levels ◽

Carbohydrate Antigen ◽

Cell Counts ◽

Blood Cell Counts ◽

Serum Carbohydrate Antigen ◽

The Difference ◽

White Blood Cell Counts

Aims. Choledocholithiasis is a common and yet potentially debilitating disease of the biliary tract. While certain patients with this disease remain largely asymptomatic or experience mild discomfort, in several cases, patient can suffer biliary inflammation and other serious symptoms. Previous studies have detected elevated serum levels of carbohydrate antigen 199 in patients with choledocholithiasis. We wanted to know whether serum CA199 level in patients with choledocholithiasis is related to the level of inflammation in patients. Methods. In the present study, we separated a cohort of 135 choledocholithiasis patients into two groups based on their white blood cell counts, which were either 3.5−9.5×109/L or ≥9.5×109/L. We also divided patients into two groups according to CPR < 10 mg/L and CRP ≥ 10 mg/L. At the same time, the correlation between CA199 and CRP level was analyzed. Results. We then used a Rank-sum test to compare serum carbohydrate 199 levels between these groups, revealing significantly higher levels of this antigen in patients with a white cell count ≥9.5×109/L (Z=−3.584, P<0.01). The two groups were grouped by CRP, and the CA199 level was compared. The difference between the two groups was statistically significant (P<0.01). The correlation analysis between CA199 and CRP showed an obvious correlation (r=0.574). Conclusion. This suggests that in patients with choledocholithiasis, higher circulating carbohydrate antigen 199 levels may correspond to a higher degree of inflammation.

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