scholarly journals Clonality Analysis ofHelicobacter pyloriin Patients Isolated from Several Biopsy Specimens and Gastric Juice in a Japanese Urban Population by Random Amplified Polymorphic DNA Fingerprinting

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Nariaki Toita ◽  
Shin-ichi Yokota ◽  
Nobuhiro Fujii ◽  
Mutsuko Konno
Keyword(s):  
Gastric Juice ◽  
Urban Population ◽  
Random Amplified Polymorphic Dna ◽  
Clonal Diversity ◽  
Japanese Patients ◽  
Upper Gastrointestinal Endoscopy ◽  
Rapd Fingerprinting ◽  
Biopsy Specimens ◽  
Single Host ◽  
H Pylori

Background. The number ofHelicobacter pyloriclones infecting a single host has been discussed in numerous reports. The number has been suggested to vary depending on the regions in the world.Aim. The purpose of this study was to examine the number of clones infecting a single host in a Japanese urban population.Materials and Methods. Thirty-one Japanese patients undergoing upper gastrointestinal endoscopy were enrolled in this study.H. pyloriisolates (total 104 strains) were obtained from biopsy specimens (antrum, corpus, and duodenum) and gastric juice. Clonal diversity was examined by the random amplified polymorphic DNA (RAPD) fingerprinting method.Results. The RAPD fingerprinting patterns of isolates from each patient were identical or very similar. And the isolates obtained from several patients with 5- to 9-year intervals showed identical or very similar RAPD patterns.Conclusion. Each Japanese individual of an urban population is predominantly infected with a singleH. pyloriclone.

scholarly journals Association Between Vitamin D Receptor Gene Polymorphisms and Helicobacter Pylori Infection

2020 ◽  
Vol 10 (1) ◽  
pp. 8-14
Author(s):  
Amal A. Mohamed ◽  
Shorouk Moussa ◽  
Mahmoud M. Shaheen ◽  
Sherief Abd-Elsalam ◽  
Rehab Ahmed ◽  
...  
Keyword(s):  
Vitamin D ◽  
Helicobacter Pylori ◽  
Vitamin D Receptor ◽  
Gene Polymorphisms ◽  
Gastrointestinal Symptoms ◽  
Upper Gastrointestinal Endoscopy ◽  
Positive Group ◽  
Biopsy Specimens ◽  
H Pylori ◽  
Upper Gastrointestinal

Background & Aims: Human genetic polymorphism has been reported in the susceptibility and clinical development of infection. In this regard, this study aimed to investigate the link between Vitamin D Receptor (VDR) gene polymorphism and H. pylori infection. Materials and Methods: This cross-sectional study was conducted on 224 adult patients with upper gastrointestinal symptoms who underwent an upper gastrointestinal endoscopy between July 2017 and May 2019 in two major university hospitals. All patients were evaluated for helicobacter pylori infection. Two gastric antral biopsy specimens were taken from each patient. One of those Biopsy specimens was evaluated for histopathology examination and the other one was immersed in a saline solution ready for genomic DNA extraction. Results: There were statistically significant differences between different genotypes of VDR rs7975232 polymorphism between H. pylori infected and non-infected groups (CC was higher in H. pylori negative and AC and AA were the most common in H. pylori positive group). There were statistical differences between different genotypes of VDR rs2228570 between H. pylori infected and non-infected groups (TT was higher in H. pylori negative and CT and CC were the most common in H. pylori positive group). Regarding VDR rs 7975232 gene polymorphisms; the (A) allele was significantly higher H. pylori infected, while (C) allele was significantly higher in uninfected patients. Regarding VDR rs 2228570 gene polymorphisms; the (C) allele was significantly higher H. pylori infected, while (T) allele was significantly higher in uninfected patients. Conclusion: There is a possible association between the FokI and Apal VDR polymorphism and H. pylori infection.

scholarly journals Increasing antimicrobial resistance of Helicobacter pylori infection among Saudi patients undergoing upper gastrointestinal endoscopy

2017 ◽  
Vol 11 ◽  
Author(s):  
Fahad A.S. Al-Eidan ◽  
Mohamed Alnaser ◽  
Sameera M. Aljohani ◽  
Mishal Al Johani ◽  
Ahmed El Hafi ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antimicrobial Susceptibility ◽  
Gastrointestinal Endoscopy ◽  
Upper Gastrointestinal Endoscopy ◽  
Ulcer Disease ◽  
Antimicrobial Susceptibility Pattern ◽  
Biopsy Specimens ◽  
H Pylori ◽  
Upper Gastrointestinal ◽  
Saudi Patients

There is growing concern about <em>Helicobacter pylori</em> (<em>H. pylori</em>) resistance strains being the main agent contributing to eradication failure. In this study we evaluated the prevalence and antimicrobial susceptibility trends of <em>H. pylori</em> over a period of time. Over a period of seven years a total of 384 biopsy specimens were sent to the microbiology department and evaluated for culturing <em>H. pylori</em> and assess its susceptibility. Biopsy specimens that cultured and where bacterial susceptibility was determined were regarded as <em>H. pylori</em> positive, and specimens that failed to culture were considered <em>H. pylori</em> negative. A total of 221 patients with <em>H. pylori</em> positive were assessed and antimicrobial susceptibility was determined. The overall prevalence of <em>H. pylori</em> infections among Saudis undergoing upper gastrointestinal endoscopy, with indicated specimens, was 57.6%. Antimicrobial susceptibility was evaluated; overall clarithromycin resistance was found to be 4.5% and metronidazole resistance 52.5%. The number of clarithromycin resistant strains has increased significantly from 2.5% in 2008 to 10% in 2014 (PH. pylori infection is very common among Saudi patients with peptic ulcer disease. Resistance of <em>H. pylori</em> against clarithromycin and metronidazole has increased significantly over the seven-year period. This suggests a need to monitor the annual antimicrobial susceptibility pattern.

Isolated Gastric Malakoplakia: A Case Report and Review of the Literature

2004 ◽  
Vol 128 (11) ◽  
pp. e153-e156 ◽  
Author(s):  
Lastra-Camacho Gustavo ◽  
Marie E. Robert ◽  
Laura W. Lamps ◽  
Suzanne P. Lagarde ◽  
Dhanpat Jain
Keyword(s):  
Yersinia Pseudotuberculosis ◽  
African American Woman ◽  
American Woman ◽  
Gastric Body ◽  
Upper Gastrointestinal Endoscopy ◽  
Fundic Gland ◽  
Biopsy Specimens ◽  
Polymerase Chain ◽  
H Pylori

Abstract A 62-year-old African American woman presented with weight loss and dyspepsia. She did not have any clinical evidence of immunodeficiency. Upper gastrointestinal endoscopy revealed multiple small polypoid lesions in the gastric body and fundus that appeared larger and more erythematous than usual fundic gland polyps. Examination of biopsy specimens revealed an infiltrate of large histiocytes with eosinophilic granular cytoplasm located in the lamina propria and containing Michaelis-Gutmann bodies. These histologic findings were diagnostic of gastric malakoplakia. Gastrointestinal malakoplakia is uncommon, and exclusive gastric involvement is extremely rare. Because occult bacterial infection has been postulated as the underlying cause of malakoplakia, the presence of Helicobacter pylori infection was investigated using immunohistochemical and serologic techniques, and the presence of Yersinia enterocolitica or Yersinia pseudotuberculosis infection was investigated by polymerase chain reaction assay. There was no evidence of H pylori, Y enterocolitica, or Y pseudotuberculosis in these biopsy specimens, and there was no evidence of malakoplakia or concurrent malignancy at any other site. Follow-up examination 12 months later revealed no endoscopic or histologic improvement.

scholarly journals A case of Russell body gastritis with multifocal lesions

2020 ◽  
Vol 8 ◽  
pp. 2050313X2092384
Author(s):  
Michinobu Umakoshi ◽  
Ken Miyabe ◽  
Hajime Ishii ◽  
Yukitsugu Kudo-Asabe ◽  
Yukinobu Ito ◽  
...  
Keyword(s):  
Endoscopic Submucosal Dissection ◽  
Histological Examination ◽  
Gastrointestinal Endoscopy ◽  
Eradication Therapy ◽  
Upper Gastrointestinal Endoscopy ◽  
Submucosal Dissection ◽  
Biopsy Specimens ◽  
Multifocal Lesions ◽  
H Pylori ◽  
Upper Gastrointestinal

Russell body gastritis is an extremely rare gastritis characterized by abundant infiltration of plasma cells with Russell body and eccentric nuclei, known as Mott cells. An 81-year-old Japanese woman with Helicobacter pylori and hepatitis C virus infection complaining of abdominal discomfort underwent upper gastrointestinal endoscopy, which detected an elevated lesion 2 cm in diameter at the anterior wall of the gastric body. A histological examination of the lesion revealed the infiltration of numerous Mott cells with an abundant eosinophilic crystal structure and eccentric nuclei in the lamina propria, resulting in a pathological diagnosis of Russell body gastritis. Endoscopic submucosal dissection (ESD) was performed subsequently. The histological findings of the resected specimen were compatible with those of Russell body gastritis. Upper gastrointestinal endoscopy performed 2 months after endoscopic submucosal dissection revealed the presence of new multiple flat elevated lesions in the antrum up to 1 cm in diameter, distant from the site of endoscopic submucosal dissection. A histological examination revealed a few Mott cells in the biopsy specimens taken from the new lesions. In turn, H. pylori eradication therapy was performed 1 month after the detection of the new lesions. One year after the eradication therapy, follow-up upper gastrointestinal endoscopy revealed that multiple lesions had almost disappeared, and the histological examination of the gastric biopsy specimens confirmed the disappearance of Mott cells. We herein report a case of Russell body gastritis in which multifocal lesions were observed after endoscopic submucosal dissection, and which was subsequently treated by H. pylori eradication therapy.

Role of vitamin C in gastric cancer

2018 ◽  
Vol 01 (1) ◽  
Author(s):  
Takalkar U Vidyadhar
Keyword(s):  
Gastric Cancer ◽  
Vitamin C ◽  
Gastric Juice ◽  
Oxidative Dna Damage ◽  
Preventive Measure ◽  
Dietary Factors ◽  
Preventive Strategy ◽  
H Pylori

Gastric cancer is a multifactorial disease with complex interplay of environmental and genetic factors. Helicobacter pylori (H. pylori) infestation has been identified as the most important etiological agent in the pathogenesis of gastric cancer. Also, the role of dietary factors that is low consumption of fruits and vegetables have been found to be associated with gastric cancer. Among the dietary factors, antioxidants especially vitamin C has been found to confer the strongest protection against gastric cancer. Its anti-proliferative and pro-apoptotic action has been suggested in vitro. Because of its antioxidant activity, it protects cells against oxidative DNA damage caused by toxic effects of reactive oxygen species. It also inhibits production of carcinogenic N-nitroso compound in the stomach. The person with H. pylori infection has low levels of vitamin C in their gastric juice and levels of vitamin C normalizes on eradication of H. pylori. Vitamin C levels are high in gastric mucosa and gastric juice, sometimes more than that of in plasma. But gastric pathological conditions cause lowered secretion of vitamin C into gastric juice. Effect of H. pylori on vitamin C in gastric juice is reversible and on eradication of H. pylori, it returns to normal level. Hence, eradication of H. pylori and chemoprevention with antioxidant supplementation will be an effective preventive strategy to reduce the incidence of gastric cancer and related mortality. Vitamin C and gastric cancer is an area of potential interest for researchers as a preventive measure. Keywords: Vitamin C, H. pylori, gastric cancer.

Inflammatory cell numbers in the stomach of Japanese subjects with endoscopically normal mucosa without H. pylori infection

2021 ◽  
Author(s):  
Naoki Sumi ◽  
Ken Haruma ◽  
Tomoari Kamada ◽  
Mitsuhiko Suehiro ◽  
Noriaki Manabe ◽  
...  
Keyword(s):  
Inflammatory Cells ◽  
Normal Mucosa ◽  
Mucosal Inflammation ◽  
Upper Gastrointestinal Endoscopy ◽  
Fundic Gland ◽  
Eosinophilic Gastritis ◽  
Pyloric Mucosa ◽  
Sydney System ◽  
Updated Sydney System ◽  
H Pylori

Introduction: Since inflammatory cells, such as lymphocytes and plasma cells, normally inhabit the stomach, the border between normal and mild inflammation is difficult to visually determine using the updated Sydney system scale of gastritis. Additionally, eosinophils in the gastric mucosa must be counted to diagnose eosinophilic gastritis. We aimed to determine the normal number of inflammatory cells in patients with endoscopically normal mucosa and without H. pylori infections. Methods: We assessed patients aged 20–79 years, who had undergone upper gastrointestinal endoscopy at Kawasaki Medical School Hospital between January 2010 and December 2014. Inflammatory cells were counted in 1,000 μm2 fields of pyloric and fundic gland mucosal biopsy specimens. We finally included 325 (male, n = 141; female, n = 184; average age = 49.3 years) patients without inflammation who had H. pylori-negative endoscopic results and negative histological findings interpreted based on the updated Sydney System and the Kyoto classification of gastritis. Results: The average numbers of nucleated cells were 83.3 ± 14.2/mm2 and 65.4 ± 12.6/mm2 in the pyloric and fundic gland mucosae, respectively. Inflammatory cells were significantly more abundant in the pyloric mucosa than the fundic gland mucosa (p < 0.05). Age and sex distribution did not significantly differ. Eosinophils were absent or scanty in the gastric mucosae of both glands in all patients. Conclusion: We determined the absolute values of inflammatory cells, including eosinophils, in normal mucosae of pyloric and fundic glands. These findings could be important in defining gastric mucosal inflammation, including eosinophilic gastritis diagnosis.

scholarly journals Incidence of Duodenal Ulcers and Gastric Ulcers in a Western Population: Back to Where It Started

2009 ◽  
Vol 23 (9) ◽  
pp. 604-608 ◽  
Author(s):  
Marcel JM Groenen ◽  
Ernst J Kuipers ◽  
Bettina E Hansen ◽  
Rob J Th Ouwendijk
Keyword(s):  
Gastric Ulcers ◽  
Bleeding Complications ◽  
Upper Gastrointestinal Endoscopy ◽  
Peptic Ulcers ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Treatment Regimens ◽  
H Pylori ◽  
Upper Gastrointestinal ◽  
Over Time

BACKGROUND/OBJECTIVES: As recently as 40 years ago, a decline in the incidence of peptic ulcers was observed. The discovery of Helicobacter pylori had a further major impact on the incidence of ulcer disease. Our aim was to evaluate the trends in the incidence and bleeding complications of ulcer disease in the Netherlands.METHODS: From a computerized endoscopy database of a district hospital, the data of all patients who underwent upper gastrointestinal endoscopy from 1996 to 2005 were analyzed. The incidence of duodenal and gastric ulcers, with and without complications, were compared over time.RESULTS: Overall, 20,006 upper gastrointestinal endoscopies were performed. Duodenal ulcers were diagnosed in 696 (3.5%) cases, with signs of bleeding in 158 (22.7%). Forty-five (6.5%) of these ulcers were classified as Forrest I and 113 (16.2%) as Forrest II. Gastric ulcers were diagnosed in 487 cases (2.4%), with signs of bleeding in 60 (12.3%). A Forrest 1 designation was diagnosed in 19 patients (3.9%) and Forrest 2 in 41 patients (8.4%). The incidence of gastric ulcers was stable over time, while the incidence of duodenal ulcers declined.CONCLUSIONS: The incidence of duodenal ulcer disease in the Dutch population is steadily decreasing over time. Test and treatment regimens for H pylori have possibly contributed to this decline. With a further decline in the prevalence of H pylori, the incidence of gastric ulcers is likely to exceed the incidence of duodenal ulcers in the very near future, revisiting a similar situation that was present at the beginning of the previous century.

scholarly journals Corpus gastritis is protective against reflux oesophagitis

Gut ◽  
1999 ◽  
Vol 45 (2) ◽  
pp. 181-185 ◽  
Author(s):  
H B El-Serag ◽  
A Sonnenberg ◽  
M M Jamal ◽  
J M Inadomi ◽  
L A Crooks ◽  
...  
Keyword(s):  
Reflux Oesophagitis ◽  
Smoking Status ◽  
Infection Rates ◽  
Multivariate Logistic Regression ◽  
Biopsy Specimens ◽  
Gastric Corpus ◽  
H Pylori ◽  
Corpus Gastritis ◽  
Reduced Risk ◽  
Reflux Œsophagitis

BACKGROUNDGastric acid is important in the pathogenesis of reflux oesophagitis. Acid production by the gastric corpus is reduced in corpus gastritis.AIMSTo determine whether corpus gastritis protects against reflux oesophagitis.METHODSPatients presenting for elective oesophagogastroduodenoscopy were studied. Two biopsy specimens were taken from the antrum, corpus, and cardia and stained with haematoxylin/eosin and Diff-Quick II stains. The presence and severity of gastritis were graded according to a modified updated Sydney classification.RESULTSOf 302 patients, 154 had endoscopic signs of reflux oesophagitis. There was no difference between patients with and controls without oesophagitis in the overall infection rates with Helicobacter pylori. Acute or chronic corpus gastritis occurred less often in patients with than those without reflux oesophagitis. Compared with controls, corpus gastritis was less severe in patients with reflux oesophagitis. The presence of acute or chronic gastritis in the corpus was significantly correlated with either type of gastritis in other areas of the stomach. In a multivariate logistic regression, age, sex, smoking status, and the presence of chronic corpus gastritis all exerted a significant influence on the presence of reflux oesophagitis. Chronic corpus gastritis was associated with a 54% reduced risk for reflux oesophagitis.CONCLUSIONSWhile infection withH pylori alone may not affect the occurrence of reflux oesophagitis, the development of chronic corpus gastritis seems to be protective.

scholarly journals Epidemiological and Clinical-Pathological Aspects of Helicobacter pylori Infection in Brazilian Children and Adults

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Evelyn Pedroso Toscano ◽  
Fernanda Fernandes Madeira ◽  
Mayra Pinheiro Dutra-Rulli ◽  
Luiz Otavio Maia Gonçalves ◽  
Marcela Alcântara Proença ◽  
...  
Keyword(s):  
Risk Factors ◽  
Pediatric Population ◽  
Adult Population ◽  
Developed Countries ◽  
Upper Gastrointestinal Endoscopy ◽  
Only Children ◽  
Screening Programs ◽  
Adolescents And Adults ◽  
H Pylori ◽  
Over 40 Years

Aim. To evaluate the prevalence and risk factors of H. pylori infection in the pediatric and adult population seen at a public hospital in São José do Rio Preto, SP, Brazil. Methods. This is a retrospective study that evaluated 2406 medical records of children, adolescents, and adults with dyspeptic symptoms who underwent upper gastrointestinal endoscopy. H. pylori diagnosis and demographic and clinical-pathological features were recorded. Results. A total of 852 subjects were H. pylori positive, with an overall prevalence of infection of 35.4%, occurring mainly in adults over 40 years of age, and a 24.7% prevalence considering only children and adolescents. No association was observed between H. pylori infection and risk factors. However, the H. pylori positive individuals showed a higher frequency of pangastritis (p<0.01), severe lesions (p=<0.001), and erosive lesions (p=0.04). The bacterium was eradicated in 83.5% (127) of the patients who received the standard therapy. Conclusions. The prevalence of H. pylori detected in a public service in São José do Rio Preto, SP, Brazil, is as expected for developed countries, showing growing rates with increasing age. As H. pylori infection occurs during childhood, screening programs for detection and prevention in the pediatric population are important to reduce the prevalence of this infection in adults.

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