scholarly journals Cyclin B1 Destruction Box-Mediated Protein Instability: The Enhanced Sensitivity of Fluorescent-Protein-Based Reporter Gene System

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Chao-Hsun Yang ◽  
Wan-Ting Kuo ◽  
Yun-Ting Chuang ◽  
Cheng-Yu Chen ◽  
Chih-Chien Lin
Keyword(s):  
Cell Cycle ◽  
Reporter Gene ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Ubiquitin Proteasome System ◽  
Cyclin B1 ◽  
Gene System ◽  
Enhanced Sensitivity ◽  
Reporter Gene System ◽  
Destruction Box

The periodic expression and destruction of several cyclins are the most important steps for the exact regulation of cell cycle. Cyclins are degraded by the ubiquitin-proteasome system during cell cycle. Besides, a short sequence near the N-terminal of cyclin B called the destruction box (D-box; CDB) is also required. Fluorescent-protein-based reporter gene system is insensitive to analysis because of the overly stable fluorescent proteins. Therefore, in this study, we use human CDB fused with both enhanced green fluorescent protein (EGFP) at C-terminus and red fluorescent protein (RFP, DsRed) at N-terminus in the transfected human melanoma cells to examine the effects of CDB on different fluorescent proteins. Our results indicated that CDB-fused fluorescent protein can be used to examine the slight gene regulations in the reporter gene system and have the potential to be the system for screening of functional compounds in the future.

Application of P450RGS reporter gene system as a bioindicator of sediment PAH contamination in the vicinity of Incheon Harbor, Korea

Biomarkers ◽  
1997 ◽  
Vol 2 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Gi Beum Kim ◽  
Jack W. Anderson ◽  
Kristen Bothner ◽  
Jong Hyeon Lee ◽  
Chul Hwan Koh ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Gene System ◽  
Reporter Gene System

Basic Evaluation of FES-hERL PET Tracer-Reporter Gene System for In Vivo Monitoring of Adenoviral-Mediated Gene Therapy

2008 ◽  
Vol 10 (5) ◽  
pp. 245-252 ◽  
Author(s):  
Talakad Goolaiah Lohith ◽  
Takako Furukawa ◽  
Tetsuya Mori ◽  
Masato Kobayashi ◽  
Yasuhisa Fujibayashi
Keyword(s):  
Gene Therapy ◽  
Reporter Gene ◽  
In Vivo Monitoring ◽  
Gene System ◽  
Pet Tracer ◽  
Reporter Gene System ◽  
Basic Evaluation

Potential of the FES–hERL PET reporter gene system — Basic evaluation for gene therapy monitoring

2006 ◽  
Vol 33 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Takako Furukawa ◽  
Talakad G. Lohith ◽  
Shinji Takamatsu ◽  
Tetsuya Mori ◽  
Takeshi Tanaka ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Reporter Gene ◽  
Therapy Monitoring ◽  
Gene System ◽  
Reporter Gene System ◽  
Basic Evaluation

629 A high-throughput cell-based reporter gene system for measuring CYP1A1 induction by xenobiotics in drinking water

2003 ◽  
Vol 144 ◽  
pp. s169
Author(s):  
E. Chiesara ◽  
S. Frigerio ◽  
R. Fumagalli ◽  
L. Marabini ◽  
S. Radice ◽  
...  
Keyword(s):  
Drinking Water ◽  
Reporter Gene ◽  
High Throughput ◽  
Gene System ◽  
Cyp1a1 Induction ◽  
Reporter Gene System

scholarly journals Analysis of Promoter Function in Aspergillus fumigatus

2012 ◽  
Vol 11 (9) ◽  
pp. 1167-1177 ◽  
Author(s):  
Sanjoy Paul ◽  
J. Stacey Klutts ◽  
W. Scott Moye-Rowley
Keyword(s):  
Aspergillus Fumigatus ◽  
Reporter Gene ◽  
Reporter Genes ◽  
Antifungal Drugs ◽  
Luciferase Reporter ◽  
Pcr Analysis ◽  
Luciferase Reporter Gene ◽  
Content Type ◽  
Gene System ◽  
Reporter Gene System

ABSTRACTThe filamentous fungusAspergillus fumigatusis an important opportunistic pathogen that can cause high mortality levels in susceptible patient populations. The increasing dependence on antifungal drugs to controlA. fumigatushas led to the inevitable acquisition of drug-resistant forms of this pathogen. In other fungal pathogens, drug resistance is often associated with an increase in transcription of genes such as ATP-binding cassette (ABC) transporters that directly lead to tolerance to commonly employed antifungal drugs. InA. fumigatus, tolerance to azole drugs (the major class of antifungal) is often associated with changes in the sequence of the azole target enzyme as well as changes in the transcription level of this gene. The target gene for azole drugs inA. fumigatusis referred to ascyp51A. In order to dissect transcription ofcyp51Atranscription and other genes of interest, we constructed a set of firefly luciferase reporter genes designed for use inA. fumigatus. These reporter genes can either replicate autonomously or be targeted to thepyrGlocus, generating an easily assayable uracil auxotrophy. We fused eight differentA. fumigatuspromoters to luciferase. Faithful behaviors of these reporter gene fusions compared to their chromosomal equivalents were evaluated by 5′ rapid amplification of cDNA ends (RACE) and quantitative reverse transcription-PCR (qRT-PCR) analysis. We used this reporter gene system to study stress-regulated transcription of a Hsp70-encoding gene, map an important promoter element in thecyp51Agene, and correct an annotation error in the actin gene. We anticipate that this luciferase reporter gene system will be broadly applicable in analyses of gene expression inA. fumigatus.

Comparative analysis of sediment extracts from NOAA's bioeffects studies by the biomarker, P450 Reporter Gene System

1999 ◽  
Vol 48 (4-5) ◽  
pp. 407-425 ◽  
Author(s):  
J.W. Anderson ◽  
J.M. Jones ◽  
J. Hameedi ◽  
E. Long ◽  
R.H. Tukey
Keyword(s):  
Comparative Analysis ◽  
Reporter Gene ◽  
Gene System ◽  
Reporter Gene System

Using an RSP3 reporter gene system to investigate molecular regulation of hydrogenase expression in Chlamydomonas reinhardtii

2013 ◽  
Vol 2 (4) ◽  
pp. 341-351 ◽  
Author(s):  
Xiaoqing Sun ◽  
Susan Perera ◽  
Nancy Haas ◽  
Paul A. Lefebvre ◽  
Carolyn D. Silflow
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Reporter Gene ◽  
Molecular Regulation ◽  
Gene System ◽  
Reporter Gene System
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