scholarly journals Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Zhenghua Song
Keyword(s):  
Uric Acid ◽  
Food Intake ◽  
Human Serum ◽  
Flow Injection ◽  
Human Urine ◽  
Inhibitory Effect ◽  
System A ◽  
Flow Injection Chemiluminescence ◽  
Sensitive Method

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

scholarly journals Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiangman Liu ◽  
Huan Yang ◽  
Yun Zhang ◽  
Min Wu ◽  
Haixiang Zhao ◽  
...  
Keyword(s):  
Human Serum ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence ◽  
Serum And Urine

A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL-1, giving the limit of detection (LOD) of 0.03 pg mL-1 (3σ). At a flow rate of 2.0 mL min-1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.

scholarly journals Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Xin Li ◽  
Jiangman Liu ◽  
Zhenghua Song
Keyword(s):  
Dissolved Oxygen ◽  
Human Serum ◽  
Flow Injection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Sampling Efficiency ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence

A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented. It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system. The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 (3σ) and the relative standard deviations lower than 2.1%. At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1. The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8.1±0.2%. The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.

Determination of risperidone at picogram level in human urine by luminol—H2O2 chemiluminescence

2006 ◽  
Vol 60 (4) ◽  
Author(s):  
X. Xie ◽  
X. Shao ◽  
Z. Song
Keyword(s):  
Flow Injection ◽  
Human Urine ◽  
High Sensitivity ◽  
Chemiluminescence Method ◽  
Synergistic Enhancement ◽  
Relative Standard ◽  
Sensitive Determination ◽  
Flow Injection Chemiluminescence ◽  
Simple Flow

AbstractA simple flow-injection chemiluminescence method with synergistic enhancement has been investigated for the rapid and sensitive determination of antipsychotic risperidone. The synergistic action was significant in the chemiluminescence system of luminol—hydrogen peroxide with risperidone as an enhancer. The increased chemiluminescence intensity was correlated with risperidone concentration within the range from 10 pg mL−1 to 1.0 ng mL−1 with relative standard deviations lower than 5.0 % and the detection limit of 4 pg mL−1. At a flow rate of 2.0 mL min−1, the flow-injection chemiluminescence method exhibited both a high sensitivity and excellent selectivity giving a throughput of 120 times per hour. The proposed method was successfully applied to determine the risperidone content in human urine without any pretreatment. It was found that the excretive amounts of risperidone reached their maximum after taking 2.0 mg of risperidone for 1 h, with a total excretive ratio of 17.37 % in 8.5 h.

Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence

Luminescence ◽  
2012 ◽  
Vol 28 (3) ◽  
pp. 407-411 ◽  
Author(s):  
Wenwen Liu ◽  
Liangxiao Xie ◽  
Hongshuang Liu ◽  
Shichao Xu ◽  
Bingcheng Hu ◽  
...  
Keyword(s):  
Human Serum ◽  
Flow Injection ◽  
Pharmaceutical Preparations ◽  
Estradiol Valerate ◽  
Flow Injection Chemiluminescence
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