scholarly journals Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Xin Li ◽  
Jiangman Liu ◽  
Zhenghua Song
Keyword(s):  
Dissolved Oxygen ◽  
Human Serum ◽  
Flow Injection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Sampling Efficiency ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence

A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented. It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system. The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 (3σ) and the relative standard deviations lower than 2.1%. At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1. The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8.1±0.2%. The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.

scholarly journals Determination of Picogram Levels of Roxithromycin in Pharmaceutical, Human Serum, and Urine by Flow-Injection Chemiluminescence

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Jiangman Liu ◽  
Huan Yang ◽  
Yun Zhang ◽  
Min Wu ◽  
Haixiang Zhao ◽  
...  
Keyword(s):  
Human Serum ◽  
Flow Injection ◽  
Limit Of Detection ◽  
Inhibitory Effect ◽  
Injection System ◽  
Relative Standard ◽  
Flow Injection System ◽  
Flow Injection Chemiluminescence ◽  
Serum And Urine

A sensitive chemiluminescence (CL) method, based on the inhibitory effect of roxithromycin (ROX) on the CL reaction between luminol and dissolved oxygen in a flow-injection system, was first proposed for the determination of ROX at picogram levels. The decrement of CL intensity was linearly proportional to the logarithm of ROX concentrations ranging from 0.1 to 100 pg mL-1, giving the limit of detection (LOD) of 0.03 pg mL-1 (3σ). At a flow rate of 2.0 mL min-1, a complete analytical procedure including sampling and washing could be performed within 0.5 min, with relative standard deviations (RSDs) of less than 5.0% (n=5). The proposed procedure was applied successfully to the determination of ROX in pharmaceutical, human serum, and urine with the recoveries ranging from 90.0 to 110.0%.

Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system

2007 ◽  
Vol 61 (5) ◽  
Author(s):  
X. Shao ◽  
H. Liu ◽  
X. Gao ◽  
W. Chen ◽  
Z. Song
Keyword(s):  
Dissolved Oxygen ◽  
Calibration Graph ◽  
Injection System ◽  
Chemiluminescence Method ◽  
Relative Standard ◽  
Flow Injection System ◽  
Human Urine And Serum ◽  
Urine And Serum ◽  
Mechanism Of The Reaction

AbstractA sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from 0.4 ng mL−1 to 400.0 ng mL−1 (r 2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals, human urine, and serum. Possible mechanism of the reaction was also discussed.

scholarly journals Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jiajia Wang ◽  
Xijuan Tan ◽  
Zhenghua Song
Keyword(s):  
Uric Acid ◽  
Food Intake ◽  
Human Serum ◽  
Flow Injection ◽  
Human Urine ◽  
Inhibitory Effect ◽  
System A ◽  
Flow Injection Chemiluminescence ◽  
Sensitive Method

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

scholarly journals Ultrasensitive determination for picomolar-level midecamycin in human serum by flow injection chemiluminescence using luminol–BSA system

2011 ◽  
Vol 26 (6) ◽  
pp. 349-355 ◽  
Author(s):  
Hairu Lv ◽  
Xijuan Tan ◽  
Yun Zhang ◽  
Zhenghua Song
Keyword(s):  
Standard Deviation ◽  
Detection Limit ◽  
Human Serum ◽  
Flow Rate ◽  
Flow Injection ◽  
Inhibitory Effect ◽  
Analysis Procedure ◽  
Serum Samples ◽  
Relative Standard ◽  
Flow Injection Chemiluminescence

An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol · l–1with a detection limit as low as 0.3 pmol · l–1(3σ). The relative standard deviation of seven repetitive measurements for 10 pmol · l–1MID was 3.0%. At a flow rate of 2.0 ml · min–1, the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h–1. This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given.

scholarly journals Flow injection analysis of nitrogen dioxide using a galvanic detector

1998 ◽  
Vol 20 (1) ◽  
pp. 17-21
Author(s):  
Shan-jun Liu ◽  
Han-xi Shen ◽  
Jian-xing Feng ◽  
M. Tubino
Keyword(s):  
Nitrogen Dioxide ◽  
Flow Injection ◽  
Sample Volume ◽  
Injection System ◽  
Flow Monitoring ◽  
Relative Standard ◽  
Flow Injection System ◽  
Gaseous Sample ◽  
Monitoring Application

A flow injection configuration (FIA) based on a galvanic detector for the determination of nitrogen dioxide is described. The gaseous sample is directly injected into a gaseous carrier. The sample is transported toward the detector. The steady state measurements are not required to obtain the reproducible peak signals. The features of FIA are compared with that of continuous flow monitoring application. The flow injection system is simple, rapid and capable of detecting NO2in the range of 1-500 ppm (v/v). The measuring range and sensitivity of the galvanic detector in FIA depend on the sample volume. A relative standard deviation is 2.4%(n=10)for 200 ppm (v/v) of nitrogen dioxide. The sampling frequency is about 24 h-1

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