scholarly journals Molecular Detection, Phylogenetic Analysis, and Identification of Transcription Motifs in Feline Leukemia Virus from Naturally Infected Cats in Malaysia

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Faruku Bande ◽  
Siti Suri Arshad ◽  
Latiffah Hassan ◽  
Zunita Zakaria
Keyword(s):  
Phylogenetic Analysis ◽  
Nested Pcr ◽  
Molecular Detection ◽  
Leukemia Virus ◽  
Viral Rna ◽  
Feline Leukemia Virus ◽  
Pcr Assay ◽  
Proviral Dna

A nested PCR assay was used to determine the viral RNA and proviral DNA status of naturally infected cats. Selected samples that were FeLV-positive by PCR were subjected to sequencing, phylogenetic analysis, and motifs search. Of the 39 samples that were positive for FeLV p27 antigen, 87.2% (34/39) were confirmed positive with nested PCR. FeLV proviral DNA was detected in 38 (97.3%) of p27-antigen negative samples. Malaysian FeLV isolates are found to be highly similar with a homology of 91% to 100%. Phylogenetic analysis revealed that Malaysian FeLV isolates divided into two clusters, with a majority (86.2%) sharing similarity with FeLV-K01803 and fewer isolates (13.8%) with FeLV-GM1 strain. Different enhancer motifs including NF-GMa, Krox-20/WT1I-del2, BAF1, AP-2, TBP, TFIIF-beta, TRF, and TFIID are found to occur either in single, duplicate, triplicate, or sets of 5 in different positions within the U3-LTR-gag region. The present result confirms the occurrence of FeLV viral RNA and provirus DNA in naturally infected cats. Malaysian FeLV isolates are highly similar, and a majority of them are closely related to a UK isolate. This study provides the first molecular based information on FeLV in Malaysia. Additionally, different enhancer motifs likely associated with FeLV related pathogenesis have been identified.

scholarly journals Naturally occurring feline leukemia virus subgroup A and B infections in urban domestic cats

2008 ◽  
Vol 89 (11) ◽  
pp. 2799-2805 ◽  
Author(s):  
Fabiana Magalhães Coelho ◽  
Maria Rosa Quaresma Bomfim ◽  
Fabíola de Andrade Caxito ◽  
Natália Almeida Ribeiro ◽  
Marcela Miranda Luppi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Leukemia Virus ◽  
Felis Catus ◽  
Feline Leukemia Virus ◽  
Domestic Cats ◽  
Blood Samples ◽  
Proviral Dna ◽  
Naturally Occurring ◽  
High Prevalence ◽  
Large Populations

A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.

scholarly journals Comparative Efficacy of Feline Leukemia Virus (FeLV) Inactivated Whole-Virus Vaccine and Canarypox Virus-Vectored Vaccine during Virulent FeLV Challenge and Immunosuppression

2015 ◽  
Vol 22 (7) ◽  
pp. 798-805 ◽  
Author(s):  
M. Patel ◽  
K. Carritt ◽  
J. Lane ◽  
H. Jayappa ◽  
M. Stahl ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Leukemia Virus ◽  
The United States ◽  
Viral Rna ◽  
Feline Leukemia Virus ◽  
Proviral Dna ◽  
Group A ◽  
Group B ◽  
Vectored Vaccine ◽  
Canarypox Virus

ABSTRACTFour vaccines for feline leukemia virus (FeLV) are available in the United States. This study's purpose was to compare the efficacy of Nobivac feline 2-FeLV (an inactivated, adjuvanted whole-virus vaccine) and PureVax recombinant FeLV (a live, canarypox virus-vectored vaccine) following FeLV challenge. Cats were vaccinated at 9 and 12 weeks with Nobivac feline 2-FeLV (group A,n= 11) or PureVax recombinant FeLV (group B,n= 10). Group C (n= 11) comprised unvaccinated controls. At 3 months postvaccination, cats were immunosuppressed and challenged with FeLV-A/61E. The outcomes measured were persistent antigenemia at 12 weeks postchallenge (PC) and proviral DNA and viral RNA at 3 to 9 weeks PC. Persistent antigenemia was observed in 0 of 11 cats in group A, 5 of 10 cats in group B, and 10 of 11 cats in group C. Group A was significantly protected compared to those in groups B (P< 0.013) and C (P< 0.0001). No difference was found between groups B and C (P> 0.063). The preventable fraction was 100% for group A and 45% for group B. At 9 weeks PC, proviral DNA and viral RNA were detected 1 of 11 cats in group A, 6 of 10 cats in group B, and 9 of 11 cats in group C. Nucleic acid loads were significantly lower in group A than in group C (P< 0.01). Group A had significantly lower proviral DNA loads than group B at weeks 6 to 9 (P< 0.02). The viral RNA loads were significantly lower in group A than in group B at weeks 7 to 9 (P< 0.01). The results demonstrate that Nobivac feline 2-FeLV-vaccinated cats were fully protected against persistent antigenemia and had significantly smaller amounts of proviral DNA and plasma viral RNA loads than PureVax recombinant FeLV-vaccinated cats and unvaccinated controls.

scholarly journals Prevalence of feline coronavirus, feline leukemia virus, and feline immunodeficiency virus in client-owned cats in Croatia

2021 ◽  
Vol 8 ◽  
pp. 24-38
Author(s):  
Jelena Raukar
Keyword(s):  
Veterinary Medicine ◽  
Feline Immunodeficiency Virus ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Antibody Prevalence ◽  
Blood Samples ◽  
Proviral Dna ◽  
Immunodeficiency Virus ◽  
Feline Coronavirus

This study aimed to determine prevalences for anti-FCoV antibody, FeLV antigen, FeLV proviral DNA, and anti-FIV antibody among client-owned cats from the cities of Zagreb and Varaždin in Croatia. Subjects included 106 client-owned cats tested at the Faculty of Veterinary Medicine, Vienna, Austria. Blood samples were tested with IFA for anti-FCoV antibody and IFA FCoV antibody titeres, with ELISA for FeLV p27 antigen, with PCR for FeLV proviral DNA, and with RIM for anti-FIV antibody. Prevalence of FCoV and FeLV was 41.51% and 6.60%, respectively. A coinfection with FeLV/FCoV and FIV/FCoV prevalence was 7.55% and 5.66%. No cats were coinfected with FIV and FeLV. All three viruses were detected, confirming their presence in Croatia. The seroepidemiological findings demonstrate that both feline retroviruses and feline coronavirus are important feline pathogens in Croatia.

scholarly journals Development and application of a quantitative real-time PCR assay to detect feline leukemia virus RNA

2008 ◽  
Vol 123 (1-2) ◽  
pp. 81-89 ◽  
Author(s):  
Andrea N. Torres ◽  
Kevin P. O’Halloran ◽  
Laurie J. Larson ◽  
Ronald D. Schultz ◽  
Edward A. Hoover
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Virus Rna

scholarly journals Long-term follow up of feline leukemia virus infection and characterization of viral RNA loads using molecular methods in tissues of cats with different infection outcomes

2015 ◽  
Vol 197 ◽  
pp. 137-150 ◽  
Author(s):  
A. Katrin Helfer-Hungerbuehler ◽  
Stefan Widmer ◽  
Yvonne Kessler ◽  
Barbara Riond ◽  
Felicitas S. Boretti ◽  
...  
Keyword(s):  
Virus Infection ◽  
Leukemia Virus ◽  
Molecular Methods ◽  
Viral Rna ◽  
Feline Leukemia Virus ◽  
Long Term Follow Up

scholarly journals Molecular detection of Feline Leukemia Virus in free-ranging jaguars (Panthera onca) in the Pantanal region of Mato Grosso, Brazil

2016 ◽  
Vol 20 (3) ◽  
pp. 316-317 ◽  
Author(s):  
Carla Patricia Amarante e Silva ◽  
Selma Samiko Miyazaki Onuma ◽  
Daniel Moura de Aguiar ◽  
Valéria Dutra ◽  
Luciano Nakazato
Keyword(s):  
Molecular Detection ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Panthera Onca ◽  
Mato Grosso ◽  
Free Ranging

scholarly journals Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil

2017 ◽  
Vol 29 (5) ◽  
pp. 660-668 ◽  
Author(s):  
Mariana M. Furtado ◽  
Sueli A. Taniwaki ◽  
Iracema N. de Barros ◽  
Paulo E. Brandão ◽  
José L. Catão-Dias ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Molecular Detection ◽  
Puma Concolor ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Molecular Testing ◽  
Domestic Cats ◽  
Immunodeficiency Virus ◽  
Free Ranging ◽  
Feline Coronavirus

We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars ( Panthera onca) and 1 oncilla ( Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma ( Puma concolor), and 1 jaguarundi ( Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99–100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil.

scholarly journals Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

2016 ◽  
Vol 37 (5) ◽  
pp. 3125 ◽  
Author(s):  
Juliana Marques Bicalho ◽  
Antônio Augusto Fonseca Júnior ◽  
Cairo Henrique Sousa de Oliveira ◽  
Cláudia Fideles Resende ◽  
Telissa Da Cunha Kassar ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Reproductive Performance ◽  
Bovine Leukemia Virus ◽  
Bluetongue Virus ◽  
Molecular Detection ◽  
Leukemia Virus ◽  
Dairy Herds ◽  
Direct Impact ◽  
Infectious Agents ◽  
Proviral Dna

Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.

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