scholarly journals Antinociceptive and Anti-Inflammatory Activities ofBridelia retusaMethanolic Fruit Extract in Experimental Animals

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Tekeshwar Kumar ◽  
Vishal Jain
Keyword(s):  
Acetic Acid ◽  
Animal Models ◽  
Methanolic Extract ◽  
Significant Inhibition ◽  
Dependent Manner ◽  
Paw Oedema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Wbc Count ◽  
Dose Dependent

Antinociceptive and anti-inflammatory potentials of methanolic extract ofBridelia retusafruit (BRME) were evaluated against different animal models in rodents. Antinociceptive effects of BRME were assessed in mice using the acetic acid-induced writhing and formalin test. Anti-inflammatory effects of BRME in three different doses, namely, 100, 200, and 400 mg/kg, were evaluated by utilizing different animal models representing various changes associated with inflammation, namely, carrageenan-induced paw oedema, histamine and serotonin-induced paw oedema, arachidonic acid-induced paw oedema, formalin-induced paw oedema, TPA-induced ear oedema, acetic acid-induced vascular permeability, total WBC count in paw fluid, and myeloperoxidase assay. Also BRME was phytochemically evaluated using chromatographic method. The BRME did not exhibit any signs of toxicity up to a dose of 2000 mg/kg. The extract showed statistical significant inhibition of induced nociception and inflammation in dose dependent manner. The higher dose of extract significantly inhibited pain and inflammation against control (P<0.001). HPLC results revealed the presence of gallic acid and ellagic acid as phytoconstituents in BRME and it was proven as anti-inflammatory agents. The present study scientifically demonstrated the antinociceptive and anti-inflammatory potential of fruit ofB. retusamethanolic extract. These effects may be attributed to the presence of polyphenolic phytoconstituents in the extract.

scholarly journals Comparative Evaluation of Analgesic, Anti-inflammatory and Antipyretic Effects of Synthetic Derivatives of Organoantimony (?) Ferocenyl Benzoate with Piroxicam

2016 ◽  
Vol 19 (1) ◽  
pp. 15-24
Author(s):  
Muhammad Shoaib Akhtar ◽  
Zulfiqar Khan ◽  
Muhammad Naveed Mushtaq ◽  
Muhammad Salman Akhtar
Keyword(s):  
Pharmaceutical Journal ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Standard Drug ◽  
Compound A ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Synthetic Derivatives ◽  
Dose Dependent

The current study was planned to evaluate comparative anti-inflammatory, analgesic and anti-pyretic activities of two newly synthesized organo-antimony (v) ferrocenyl benzoate derivatives with piroxicam. Anti-microbial activity of these compounds was also screened against two microorganisms. Analgesic effect of test compounds was evaluated by formalin-induced paw licking test in mice. The test compounds at 50 and 100 mg/kg b.w. doses exhibited significant (p<0.001) reduction of paw licking in treated mice comparable with standard drug piroxicam. Anti-inflammatory activity was assessed against carrageenan-induced paw oedema. The compound A produced anti-inflammatory effects comparable with standard piroxicam in dose dependent manner whereas compound B showed better effects than piroxicam at dose of 100 mg/kg body weight. To investigate anti-pyretic activity, fever was induced by administration of Brewer’s yeast in mice. Compound A showed highly significant inhibition of pyrexia (p<0.001) comparable to piroxicam after 3 hours while compound B (50 and 100 mg/kg) produced relatively lower anti-pyretic effect than standard drug. Antibacterial activity determined by disc diffusion method showed that compound B was relatively more effective than compound A against Staphylococcus aureus and Klebsiella pneumoniae. It is conceivable that both the tested compounds possessed anti-inflammatory, analgesic, anti-pyretic and anti-microbial effects even after the structural modification of parent compound.Bangladesh Pharmaceutical Journal 19(1): 15-24, 2016

scholarly journals Polyphenolic Profile, Anti-Inflammatory and Antinociceptive Activity of an Extract from Arctium lappa L. Roots

2017 ◽  
Vol 45 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Simona CONEA ◽  
Cristina MOGOSAN ◽  
Oliviu VOSTINARU ◽  
Claudia Crina TOMA ◽  
Ioana CUC HEPCAL ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Writhing Test ◽  
Arctium Lappa ◽  
Paw Oedema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Polyphenolic Profile ◽  
Dose Dependent ◽  
Rat Paw Oedema

The aim of the study was to assess the polyphenolic profile, anti-inflammatory and antinociceptive activity of Arctium lappa, a medicinal plant traditionally used in the treatment of gout, hepatitis and other inflammatory disorders. Polyphenolic profile of a hydro-glycero-ethanolic extract from Arctium lappa roots (ALE) was evaluated by HPLC-MS method. Anti-inflammatory effect of the Arctium lappa extract (ALE) was determined by carrageenan-induced rat paw oedema test, while antinociceptive effect was determined by acetic acid induced writhing test in mice and Randall Selitto test in rats. HPLC-MS analysis of the extract showed the presence of chlorogenic acid (158.9 μg/mL) and quercitrin (14.4 μg/mL). The administration of ALE reduced the oedema formation in the carrageenan-induced rat paw oedema test, especially at dose of 500 mg/kg, the results being statistically significant and dose-dependent. Also, ALE showed statistically significant and dose-dependent antinociceptive effects in the acetic acid induced writhing test in mice and Randall Selitto test in rats. The results of the pharmacological experiments suggest that the anti-inflammatory and antinociceptive effects of the Arctium lappa extract (ALE) may be related to the ability of polyphenols such as chlorogenic acid to inhibit the synthesis and release of some pro-inflammatory mediators. Our experimental data justify the traditional use of this plant in the management of some inflammatory disease.

scholarly journals Evaluation of Analgesic and Anti-inflammatory Activities of Polyalthia simiarum (Hook. F. &Thomson)

2019 ◽  
Vol 5 (1) ◽  
pp. 18-23
Author(s):  
Selina Kabir ◽  
Ronok Zahan ◽  
Abdullah Mohammad Sarwaruddin Chowdhury ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Abdur Rashid
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Biological Activities ◽  
Dependent Manner ◽  
Paw Edema ◽  
Immersion Method ◽  
Ongoing Research ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Dose Dependent

Background: Polyalthia simiarum (Hook. F. &Thomson) exhibits different effects in human body. Objective: As a part of ongoing research on medicinal plants of Bangladesh, the present study is focused to investigate the analgesic and anti-inflammatory activities of stem bark of Polyalthia simiarum (Annonaceae). Methodology: The ethyl acetate (EA) and petroleum ether (PE) extracts were subjected to qualitative chemical investigation for the identification of different phytoconstituents. The analgesic activity was determined for its central and peripheral pharmacological actions using tail immersion method and acetic acid-induced writhing test. The anti-inflammatory activity was evaluated by carrageenan induced paw edema in rats. Analgesic and anti-inflammatory data were evaluated statistically analysed by Dunnett’s-T test. Result: Both extracts at the dose of 50- and 100 mg/kg b.w., produced significant increase in pain threshold in tail immersion method whereas significantly reduced the writhing caused by acetic acid in a dose dependent manner. The EA and PE extracts showed anti-inflammatory activities at 50- and 100 mg/kg body weight. Among all the extracts, the EA extract showed a dose dependent and comparable analgesic activity in all the tested methods and also reduced the paw edema considerably (27.5% and 39.1% inhibition after 4h), in dose dependent manner when compared to carrageenan induced control rat. Conclusion: Therefore, the EA and PE extracts of Polyalthia simiarum were capable to exhibit moderate analgesic and anti-inflammatory activities. This is the first report of analgesic and anti-inflammatory potential of Polyalthia simiarum and can be further investigated to isolate the active compounds responsible for the biological activities. Journal of National Institute of Neurosciences Bangladesh, 2019;5(1): 18-23

scholarly journals The Methanolic Extract fromMurraya koenigiiL. Inhibits Glutamate-Induced Pain and Involves ATP-Sensitive K+Channel as Antinociceptive Mechanism

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Nushrat Sharmin Ani ◽  
Sudip Chakraborty ◽  
Md. Moniruzzaman
Keyword(s):  
Acetic Acid ◽  
Potassium Channels ◽  
Methanolic Extract ◽  
Scientific Basis ◽  
K Channel ◽  
Dependent Manner ◽  
Glutamatergic System ◽  
Antinociceptive Effects ◽  
Wide Range ◽  
Antinociceptive Mechanisms

Murraya koenigiiL. is a perennial shrub, belonging to the family Rutaceae. Traditionally, the leaves of this plant are extensively used in treatment of a wide range of diseases and disorders including pain and inflammation. Although researchers have revealed the antinociceptive effects of this plant’s leaves during past few years, the mechanisms underlying these effects are still unknown. Therefore, the present study evaluated some antinociceptive mechanisms of the methanolic extract ofM. koenigii(MEMK) leaves along with its antinociceptive potential using several animal models. The antinociceptive effects of MEMK were evaluated using formalin-induced licking and acetic acid-induced writhing tests at the doses of 50, 100, and 200 mg/kg. In addition, we also justified the possible participations of glutamatergic system and ATP-sensitive potassium channels in the observed activities. Our results demonstrated that MEMK significantly (p<0.01) inhibited the pain thresholds induced by formalin and acetic acid in a dose-dependent manner. MEMK also significantly (p<0.01) suppressed glutamate-induced pain. Moreover, pretreatment with glibenclamide (an ATP-sensitive potassium channel blocker) at 10 mg/kg significantly (p<0.05) reversed the MEMK-mediated antinociception. These revealed that MEMK might have the potential to interact with glutamatergic system and the ATP-sensitive potassium channels to exhibit its antinociceptive activities. Therefore, our results strongly support the antinociceptive effects ofM. koenigiileaves and provide scientific basis of their analgesic uses in the traditional medicine.

scholarly journals Antioxidant, Anti-Inflammatory, and Antidiabetic Activities of Leaves and Stems of Uapaca bojeri Bail. (EUPHORBIACEAE), an Endemic Plant of Madagascar

2020 ◽  
Vol 13 (4) ◽  
pp. 71 ◽  
Author(s):  
Zoarilala Rinah Razafindrakoto ◽  
Dario Donno ◽  
Nantenaina Tombozara ◽  
Harilala Andriamaniraka ◽  
Charles Andrianjara ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Blood Glucose ◽  
Methanol Extract ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Uapaca Bojeri

Uapaca bojeri is an endemic Malagasy plant used by the local population. This work aimed to evaluate antioxidant, anti-inflammatory, and antidiabetic activities of the methanol extracts of U. bojeri leaves and stems and to report their total phenolic content and the bioactive compound content by HPLC methods. Antioxidant capacity was determined by DPPH and ferric reducing antioxidant power (FRAP) assays. An in vivo carrageenan-induced paw oedema and acetic acid-induced writhing test in mice were used for anti-inflammatory activity evaluation. An oral glucose tolerance test was performed in mice to evaluate antidiabetic activity. The total bioactive compound content of leaves was higher than that of stems. Stem methanol extract inhibited the free radical DPPH more than the leaf methanol extract. Leaf methanol extract inhibited, in a dose-dependent manner, the carrageenan-induced paw oedema more than the stem extract, but their inhibition of the pain symptoms caused an acetic acid-induced decrease similar to the number of writhes in the dose-dependent case. The leaf and stem methanol extracts significantly reduced blood glucose levels after 30 min of glucose loading in mice compared to the control group blood glucose reduction. The presence of several bioactive compounds in U. bojeri contributed to the different biological activities, but isolation and identification of these bioactive molecules are necessary to confirm these pharmacological properties.

Attenuating Effect of Portulaca oleracea Extract on Chronic Constriction Injury Induced Neuropathic Pain in Rats: An Evidence of Anti-oxidative and Anti-inflammatory Effects

2019 ◽  
Vol 18 (4) ◽  
pp. 342-349 ◽  
Author(s):  
Fatemeh Forouzanfar ◽  
Hossein Hosseinzadeh ◽  
Mohammad B. Khorrami ◽  
Samira Asgharzade ◽  
Hassan Rakhshandeh
Keyword(s):  
Neuropathic Pain ◽  
Chronic Constriction Injury ◽  
Portulaca Oleracea ◽  
Dependent Manner ◽  
Tnf Α ◽  
Antinociceptive Effects ◽  
Drug Treatments ◽  
Thiol Content ◽  
Anti Inflammatory ◽  
Dose Dependent

Background: Neuropathic pain responds poorly to drug treatments. The present study investigated the therapeutic effect of Portulaca oleracea, in chronic constriction injury (CCI)-induced neuropathic pain in rats. Objective & Methods: Neuropathic pain was performed by putting four loose ligatures around the sciatic nerve. CCI resulted in the development of heat hyperalgesia, mechanical allodynia and cold allodynia accompanied by an increase in the contents of TNF-α, IL1β, malondialdehyde, with a reduction in total thiol content. Results: Administration of Portulaca oleracea (100 and 200 mg/kg intraperitoneal) for 14 days in CCI rats significantly alleviated pain-related behaviors, oxidative damage and inflammatory cytokines in a dose-dependent manner. Conclusion: In conclusion, it is suggested that the antinociceptive effects of Portulaca oleracea might be due to antioxidant and anti-inflammatory properties.

scholarly journals Anti-inflammatory activity of Syzygium cumini leaf against experimentally induced acute and chronic inflammations in rodents

2011 ◽  
Vol 1 (1) ◽  
pp. 6 ◽  
Author(s):  
Anirban Roy ◽  
Sanjib Bhattacharya ◽  
Jitendra N. Pandey ◽  
Moulisha Biswas
Keyword(s):  
Inflammatory Activity ◽  
Dependent Manner ◽  
Syzygium Cumini ◽  
Large Size ◽  
Paw Oedema ◽  
Chronic Models ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Dose Dependent ◽  
Rat Paw Oedema

<p><em>Syzygium cumini<strong> </strong></em>(L.) Skeels (Myrtaceae), commonly known as <em>Jam </em>in Bengali, <em>Jamun</em> in Hindi and <em>Black Plum</em> or <em>Black Berry</em> in English, is a large size evergreen tree indigenous to India and is cultivated for its fruits. In the present study, the methanol extract of leaves from <em>S. cumini </em>(MESC) was evaluated for anti-inflammatory activity in experimental acute (carrageenan, histamine and serotonin induced rat paw oedema) and chronic models (cotton pellet induced rat granuloma). In all models, the MESC (100 and 200 mg/kg body wt. p.o.) exhibited significant anti-inflammatory activity (<em>p &lt; 0.001</em>) in a dose dependent manner. These findings revealed that the <em>S. cumini </em>leaf had remarkable acute and chronic anti-inflammatory actions in the tested rodent models.</p>

scholarly journals Anti-inflammatory and antinociceptive activities of methanolic extract from red seaweed Dichotomaria obtusata

2013 ◽  
Vol 49 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Neivys García Delgado ◽  
Ana Iris Frías Vázquez ◽  
Hiran Cabrera Sánchez ◽  
Roberto Menéndez Soto del Valle ◽  
Yusvel Sierra Gómez ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Intraperitoneal Administration ◽  
Moderate Activity ◽  
Dependent Manner ◽  
Ear Edema ◽  
Antinociceptive Effects ◽  
Anti Inflammatory ◽  
Phospholipase A2 Activity

The aim of the present work was to investigate the anti-inflammatory and antinociceptive effects of methanolic extract from D. obtusata using classic models in mice (croton oil-induced ear edema and acetic acid-induced writhing) and a phospholipase A2 activity test. Qualitative analysis of the chemical composition of seaweed was also determined by extraction with solvents of increasing polarity and precipitation and color tests. Results of qualitative chemical study showed the presence of lactonic and phenolic compounds, reduced carbohydrates, other sugars, flavonoids, fatty compounds, triterpenes and steroids. The extract inhibited mouse ear edema in a dose-dependent manner with an efficacy higher than 90% and a mean effective dose of 4.87µg/ear, while intraperitoneal administration presented a moderate activity. The extract did not inhibit phospholipase A2 activity. In the writhing test, the intraperitoneal administration of the extract showed a strong antinociceptive activity (80.2%), while the oral route showed a lower efficacy. In conclusion, this study demonstrated the anti-inflammatory and antinociceptive effects of methanol extract of D. obtusata in experimental models, suggesting its therapeutic potential in the treatment of peripheral painful and/or inflammatory pathologies.

scholarly journals Chemical Composition and Antioxidant, Analgesic, and Anti-Inflammatory Effects of Methanolic Extract ofEuphorbia retusain Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Jazia Sdayria ◽  
Ilhem Rjeibi ◽  
Anouar Feriani ◽  
Sana Ncib ◽  
Wided Bouguerra ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Significant Inhibition ◽  
Paw Edema ◽  
Alternative Source ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
Anti Inflammatory

Plants provide an alternative source to manage different human disorders due to various metabolites. The aim of this study is to investigate the phytochemical constituents of the methanolic extracts ofEuphorbia retusaand to evaluate their antioxidant, anti-inflammatory, and analgesic activities. The phytochemical results obtained by HPLC and by chemical assay reactions have revealed the richness of the methanolic extract ofE. retusain active compounds, in particular polyphenols, flavonoids, and tannins. The methanolic extract shows significant antioxidant activitiesin vitro, in the DPPH and the FRAP assays. The antinociceptive activity was evaluated using acetic acid and hot-plate models of pain in mice. The anti-inflammatory activity was evaluated by carrageenan-induced paw edema. Oral pretreatment with the methanolic extract ofE. retusa(200 mg/kg) exhibited a significant inhibition of pain induced either by acetic acid or by the heating plate and in a manner comparable to the standard drug paracetamol.E. retusasignificantly reduced paw edema starting from the 3rd hour after carrageenan administration by increasing the activity of antioxidant enzymes (SOD, CAT, and GPx) in liver and paw tissues and decreasing the levels of MDA. These results may confirm the interesting potential of this plant as a treatment of various inflammatory and pain diseases.

scholarly journals Evaluation on Analgesic and Anti-Inflammatory Activities of Total Flavonoids from Juniperus sabina

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jun Zhao ◽  
Aosimanjiang Maitituersun ◽  
Chenyang Li ◽  
Qian Li ◽  
Fang Xu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Total Flavonoids ◽  
Hot Plate ◽  
Egg Albumin ◽  
Inhibition Ratio ◽  
Paw Oedema ◽  
Dependent Inhibition ◽  
Anti Inflammatory ◽  
Juniperus Sabina ◽  
Dose Dependent

The leaves of Juniperus sabina (Cupressaceae) are used in traditional Uygur medicine for the treatment of rheumatism and arthritic pain. This study aimed to investigate the analgesic and anti-inflammatory effects of total flavonoids from leaves of Juniperus sabina (JSTF) on rodents. The anti-inflammatory activity was investigated using the carrageenan, egg albumin, or histamine-induced rat paw edema as well as xylene-induced ear edema, capillary permeability, and cotton pellet granuloma while the antinociceptive activity was evaluated using the mouse writhing, formalin, and hot-plate tests. JSTF (125, 250, 500 mg/kg) significantly inhibited xylene-induced ear edema in mice (inhibition ratio as 16.22%, 40.67%, and 51.78%, respectively) and also significantly ameliorated acetic acid increased vascular permeability in mice (inhibition ratio as 11.63%, 32.56%, and 53.49%, respectively). JSTF (250 and 500 mg/kg) gave significant reduction of carrageenin-induced paw oedema at the interval of 1 h and 5 h. Administration of JSTF (500 mg/kg) caused a significant anti-inflammatory effect against oedema induced by egg albumin or histamine at the interval of 0.5 h and 4 h, and both which induced the paw oedema were also inhibited by JSTF (250 mg/kg) at a point in 1, 2, or 3 h after the inflammation. Furthermore, JSTF (125, 250, and 500 mg/kg) produced time-course increase in pain threshold in hot-plate test also. JSTF produced concentration-dependent inhibition on cyclooxygenase-2 (COX-2) or 5-lipoxygenase (5-LO) activities in vitro, and their IC50 values were 31.92 and 129.26 μg/mL, respectively. Moreover, JSTF significantly caused a significant dose-dependent inhibition on acetic acid induced writhing response in mice (inhibition ratio as 23.27%, 36.91%, and 50.76%, respectively). JSTF also significantly ameliorated formalin-induced pain in mice in the late phase on dose-dependent way. These results confirms the clinical use of J. sabina for treatment of rheumatoid arthritis in ethnomedicine, and its effective mechanism will be further studied in the future.

Sign in / Sign up

Export Citation Format

Share Document