Age-Related Differences in Percentages of Regulatory and Effector T Lymphocytes and Their Subsets in Healthy Individuals and Characteristic STAT1/STAT5 Signalling Response in Helper T Lymphocytes

The dynamic process of the development of the immune system can in itself result in age-related immune malfunctions. In this study, we analysed lymphocyte subsets in the peripheral blood of 60 healthy donors, divided into groups of children, adolescents, and adults, focusing on effector (Teff) and regulatory (Treg) T lymphocytes and STAT1/STAT5 signalling response in helper T lymphocytes (Th) in adults, using flow cytometry. Our results demonstrate a decrease in the percentage of total Tregs and an increase in the percentage of total Teffs with age and a consequential immense increase in the Teff/Treg ratio. The increase of Teffs was most apparent in Th1, Th1Th17, and Th17CD161− subsets. Significant Th lymphocyte STAT1 expression differences were observed between children and adolescents, which were associated with the decrease in activated Tregs. Higher expression of STAT1 was found in FoxP3hi than in FoxP3low Th lymphocytes, while significant IL-2 induced STAT5 phosphorylation differences were found among the subsets of Th lymphocytes in adults. Our study demonstrates age-related changes in circulating Teff and Treg, as well as significant differences in STAT5/STAT1 signalling among FoxP3+ Th lymphocytes, providing new advances in the understanding of immunosenescence.

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Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs

Developmental & Comparative Immunology ◽

10.1016/j.dci.2018.05.022 ◽

2018 ◽

Vol 87 ◽

pp. 64-74 ◽

Cited By ~ 7

Author(s):

Sita S. Withers ◽

Peter F. Moore ◽

Hong Chang ◽

Jin W. Choi ◽

Stephen J. McSorley ◽

...

Keyword(s):

Flow Cytometry ◽

Lymphocyte Subsets ◽

Color Flow ◽

Age Related ◽

Age Related Changes

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Age-stratified pediatric reference values of lymphocytes in the Moroccan population

Human Antibodies ◽

10.3233/hab-200432 ◽

2020 ◽

pp. 1-10

Author(s):

Aicha El Allam ◽

Sara El Fakihi ◽

Hicham Tahoune ◽

Karima Sahmoudi ◽

Houria Bousserhane ◽

...

Keyword(s):

T Lymphocytes ◽

Reference Values ◽

Blood Lymphocyte ◽

Healthy Subjects ◽

Lymphocyte Subsets ◽

Age Groups ◽

Lymphocyte Subpopulation ◽

Study Group ◽

Moroccan Population ◽

Helper T Lymphocytes

The number of circulating lymphocytes is altered in a number of diseases including either increase (lymphocytosis) or decrease (lymphocytopenia). Therefore, the assessment of total blood lymphocyte numbers and the relative distribution of lymphocyte subsets is a critical front-line tool in the clinical diagnosis of a number of diseases, including pediatric diseases and disorders. However, the interpretation of this data requires comparison of patient’s results to reliable reference values. Blood lymphocyte subpopulation numbers are also subject to genetic polymorphisms, immunogenic and environmental factors and vary greatly between populations. While the best practice reference values should be established within local representative populations of healthy subjects, to date, Caucasian reference values are used in Morocco due to the absence of indigenous reference values. Potential differences in blood lymphocyte subpopulation reference values between Caucasian versus Moroccan populations can adversely affect the diagnosis of pediatric and childhood diseases and disorders such as primary immunodeficiency (PID) in Morocco. OBJECTIVE: The aim of this study was to establish the age-stratified normal reference values of blood lymphocyte subsets for the pediatric Moroccan population. METHODS: We measured the concentration of lymphocyte subpopulations by flow cytometry from 83 Moroccan healthy subjects stratified into 5 age groups of 0–1, 1–2, 2–6, 6–12 and > 12–18 (adult). RESULTS: The absolute and relative amounts of the main lymphocyte subsets of T-cells, B cells and Natural Killer (NK) cells were measured and compared to previously described reference values from Cameroonian, Turkish, American and Dutch populations. Additionally, we also observed an age-related decline in the absolute population sizes of lymphocyte subsets within our study group. Relative proportions of CD3+CD4+ helper T lymphocytes decreased with increasing age and by 12 years-adult age, both proportions of CD3+CD4+ helper T lymphocytes and CD3+CD8+ cytotoxic T lymphocytes, as well as CD3-CD19+ B lymphocytes were also decreased. Finally, we compared the median values and range of our Moroccan study group with that of published results from Cameroon, Turkey, USA and Netherlands and observed significant differences in median and mean values of absolute number and relative proportions of lymphocyte subsets especially at 0–1 years and 1–2 years age groups. Above age 12 years, the Moroccan values were lower. For NK cells, the Moroccan values are also lower. CONCLUSIONS: The results of this study have a significant impact in improving the threshold values of the references intervals routinely used in the diagnosis of paediatric diseases such as PIDs or mother-to-child transmitted HIV within the Moroccan population.

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Age-related changes in T lymphocytes of patients with head and neck squamous cell carcinoma

Immunity & Ageing ◽

10.1186/s12979-020-0174-7 ◽

2020 ◽

Vol 17 (1) ◽

Cited By ~ 6

Author(s):

S. S. Jeske ◽

P. J. Schuler ◽

J. Doescher ◽

M. N. Theodoraki ◽

S. Laban ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

T Lymphocytes ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Neck Squamous Cell Carcinoma ◽

Age Related ◽

Age Related Changes

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Assessing Age-Related Changes in Peripheral Blood Leukocyte Phenotypes in Labrador Retriever Dogs Using Flow Cytometry

Journal of Nutrition ◽

10.1093/jn/132.6.1655s ◽

2002 ◽

Vol 132 (6) ◽

pp. 1655S-1657S ◽

Cited By ~ 24

Author(s):

Paul R. Heaton ◽

Daniel G. Blount ◽

Pauline Devlin ◽

Stephan Koelsch ◽

Sarah J. Mann ◽

...

Keyword(s):

Flow Cytometry ◽

Peripheral Blood ◽

Peripheral Blood Leukocyte ◽

Labrador Retriever ◽

Age Related ◽

Blood Leukocyte ◽

Age Related Changes

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Age-related reference intervals for lymphocyte subsets in whole blood of healthy individuals

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.1080/00365519950185805 ◽

1999 ◽

Vol 59 (2) ◽

pp. 89-92 ◽

Cited By ~ 29

Author(s):

S. E. McNerlan ◽

H. D. Alexander ◽

I. M. Rea

Keyword(s):

Whole Blood ◽

Lymphocyte Subsets ◽

Reference Intervals ◽

Healthy Individuals ◽

Age Related

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Assessment of age-related changes of salivary immunoglobulin a levels among healthy individuals

Journal of Pharmacy And Bioallied Sciences ◽

10.4103/jpbs.jpbs_605_20 ◽

2021 ◽

Vol 13 (5) ◽

pp. 461

Author(s):

DipakBaliram Patil ◽

ManishkumarDinkar Shete ◽

Priyatam Karade ◽

Rutuja Chopade ◽

Neha Gandhi ◽

...

Keyword(s):

Immunoglobulin A ◽

Healthy Individuals ◽

Age Related ◽

Salivary Immunoglobulin A ◽

Age Related Changes

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Circulating Exosomes Inhibit B Cell Proliferation and Activity

Cancers ◽

10.3390/cancers12082110 ◽

2020 ◽

Vol 12 (8) ◽

pp. 2110

Author(s):

Jan C. Schroeder ◽

Lisa Puntigam ◽

Linda Hofmann ◽

Sandra S. Jeske ◽

Inga J. Beccard ◽

...

Keyword(s):

Flow Cytometry ◽

Cell Proliferation ◽

B Cells ◽

B Cell ◽

Surface Expression ◽

Receptor Expression ◽

Healthy Individuals ◽

Inhibitory Effects ◽

Healthy Donors ◽

Checkpoint Receptors

(1) Background: Head and neck squamous cell carcinoma (HNSCC) is characterized by a distinctive suppression of the anti-tumor immunity, both locally in the tumor microenvironment (TME) and the periphery. Tumor-derived exosomes mediate this immune suppression by directly suppressing T effector function and by inducing differentiation of regulatory T cells. However, little is known about the effects of exosomes on B cells. (2) Methods: Peripheral B cells from healthy donors and HNSCC patients were isolated and checkpoint receptor expression was analyzed by flow cytometry. Circulating exosomes were isolated from the plasma of HNSCC patients (n = 21) and healthy individuals (n = 10) by mini size-exclusion chromatography. B cells from healthy individuals were co-cultured with isolated exosomes for up to 4 days. Proliferation, viability, surface expression of checkpoint receptors, and intracellular signaling were analyzed in B cells by flow cytometry. (3) Results: Expression of the checkpoint receptors PD-1 and LAG3 was increased on B cells from HNSCC patients. The protein concentration of circulating exosomes was increased in HNSCC patients as compared to healthy donors. Both exosomes from healthy individuals and HNSCC patients inhibited B cell proliferation and survival, in vitro. Surface expression of inhibitory and stimulatory checkpoint receptors on B cells was modulated in co-culture with exosomes. In addition, an inhibitory effect of exosomes on B cell receptor (BCR) signaling was demonstrated in B cells. (4) Conclusions: Plasma-derived exosomes show inhibitory effects on the function of healthy B cells. Interestingly, these inhibitory effects are similar between exosomes from healthy individuals and HNSCC patients, suggesting a physiological B cell inhibitory role of circulating exosomes.

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