scholarly journals Adipogenic Activity of Wild Populations ofRhododendron groenlandicum, a Medicinal Shrub from the James Bay Cree Traditional Pharmacopeia

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Michel Rapinski ◽  
Lina Musallam ◽  
John Thor Arnason ◽  
Pierre Haddad ◽  
Alain Cuerrier
Keyword(s):  
Medicinal Plant ◽  
Phenolic Content ◽  
Wild Populations ◽  
James Bay ◽  
Phenolic Profile ◽  
Murine Cell Line ◽  
James Bay Cree ◽  
Labrador Tea ◽  
Ethanol Extracts

The traditional medicinal plant, Labrador tea (Rhododendron groenlandicum(Oeder) Kron & Judd; Ericaceae), present in the pharmacopoeia of the Cree of Eeyou Istchee, has shown glitazone-like activity in the 3T3-L1 adipogenesis bioassay. This activity has been attributed to phenolic compounds, which have been shown to vary in this plant as a function of insolation parameters. The goal of this study was to determine if these changes in phenolic content were pharmacologically significant. Leaves were harvested in 2006 throughout the James Bay region of Northern Quebec and ethanol extracts were testedin vitrousing the 3T3-L1 murine cell line adipogenesis bioassay. This traditional medicinal plant was found active in the assay. However, there was no detectable spatial pattern in the accumulation of intracellular triglycerides, suggesting that such patterns previously observed in the phenolic profile of Labrador tea were not pharmacologically significant. Nonetheless, a reduction in the adipogenic activity was observed and associated with higher concentrations of quercetin for which selected environmental variables did not appropriately explain its variation.

In vitro antifilarial activity of extracts of the medicinal plant Cardiospermum halicacabum against Brugia pahangi

2000 ◽  
Vol 74 (3) ◽  
pp. 241-246 ◽  
Author(s):  
W. Khunkitti ◽  
Y. Fujimaki ◽  
Y. Aoki
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Mtt Assay ◽  
Ethanol Extract ◽  
Aqueous Extracts ◽  
Adult Males ◽  
Brugia Pahangi ◽  
Ethanol Extracts ◽  
Antifilarial Activity

AbstractThe in vitro effects of ethanol and aqueous extracts of the medicinal plant Cardiospermum halicacabum on adult worms and microfilariae of Brugia pahangi were investigated. With or without the plant extracts in culture medium, the motility of adult worms, microfilariae and microfilarial release from female worms were monitored daily. After 7 days of culture, viability or tissue damage of adult worms was assessed using the MTT assay. At > 500 μg ml-1, the aqueous extract significantly reduced motility of adult females after 24 h of exposure and adult males after 3 days. The aqueous extract, at > 500 μg ml-1, also significantly reduced microfilarial release from female worms, starting on day 2. The reduction in the motility of adult worms and the pattern of microfilarial release from female worms were concentration and time dependent. The MTT assay results revealed that adult worms cultured in the presence of aqueous extracts at > 500 μg ml-1 were damaged. However, the aqueous extract did not affect the motility of microfilariae with the exception of those in higher concentration extracts. Higher concentrations of ethanol extracts (2 mg ml-1) inhibited both the motility of adult worms and the release of microfilariae from females. Little effect of ethanol extracts was detected by the MTT assay, as only slight damage was caused to worms exposed only to the highest concentration (2 mg ml-1). However, ethanol extract at 500 μg ml-1 rapidly reduced the motility of microfilariae on day 2. The present study revealed that an aqueous extract of C. halicacabum has mild but definite direct macrofilaricidal action on B. pahangi.

scholarly journals In Vitro Study of Two Edible Polygonoideae Plants: Phenolic Profile, Cytotoxicity, and Modulation of Keap1-Nrf2 Gene Expression

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 811
Author(s):  
Marina Jovanović ◽  
Dina Tenji ◽  
Biljana Nikolić ◽  
Tatjana Srdić-Rajić ◽  
Emilija Svirčev ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Folk Medicine ◽  
In Vitro Study ◽  
Combined Approach ◽  
Phenolic Profile ◽  
Polygonum Aviculare ◽  
Qrt Pcr ◽  
Synergistic Cytotoxicity ◽  
Ethanol Extracts

Polygonum aviculare and Persicaria amphibia (subfam. Polygonoideae) are used in traditional cuisines and folk medicine in various cultures. Previous studies indicated that phytochemicals obtained from Polygonoideae plants could sensitize chemoresistant cancer cells and enhance the efficacy of some cytostatics. Here, the cytotoxic properties of chemically characterized ethanol extracts obtained from P. aviculare and P. amphibia, individually and in combination with doxorubicin (D), were determined against hepatocarcinoma HepG2 cells. Phenolic composition, cell viability, cell cycle, apoptosis, and the expression of Keap1 and Nrf2 were examined by following methods: LC-MS/MS, LC-DAD-MS, MTT, flow cytometry, and qRT-PCR. Extracts were rich in dietary polyphenolics. Synergistic cytotoxicity was detected for extracts combined with D. The observed synergisms are linked to the interference with apoptosis, cell cycle, and expression of Keap1-Nrf2 genes involved in cytoprotection. The combined approach of extracts and D could emerge as a potential pathway of chemotherapy improvement.

scholarly journals Strawberry agro-industrial by-products as a source of bioactive compounds: effect of cultivar on the phenolic profile and the antioxidant capacity

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Esteban Villamil-Galindo ◽  
Franco Van de Velde ◽  
Andrea M. Piagentini
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Profile ◽  
The Impact ◽  
By Products

AbstractThe post-harvest processing of strawberries generates considerable amounts of by-products that consist of the inedible parts of the fruit (sepal, calyx, stem, and non-marketable portion of the fruit), which is an environmental problem for local producers and industries. This study aimed to revalue these kinds of tissues through identifying and quantifying the genotype influence on the total phenolic content, phenolic profile, and the antioxidant activity of the by-products from three strawberry cultivars: ‘Festival’ (FE), ‘San Andreas ‘ (SA), and ‘Camino Real’ (CR). The total phenolic content was determined by the Folin–Ciocalteu method, in-vitro antioxidant activity by the DPPH* radical scavenging method and the phenolic profile by PAD–HPLC. The different genotypes showed significant differences (p < 0.05) in total phenolic content (TPC), FE being the one with the highest TPC (14.97 g of gallic acid equivalents < GAE > /Kg of by-product < R >), followed by SA and CR cultivars. The antioxidant capacity of the SA and FE tissues were similar (p > 0.05) and higher (15.1–16.3 mmol Trolox equivalents < TE > /Kg R) than CR. Eight main phenolic compounds were identified and quantified on the three cultivars. Agrimoniin was the principal polyphenol (0.38–1.56 g/Kg R), and the cultivar FE had the highest concentration. This compound showed the highest correlation coefficient with the antioxidant capacity (R2 0.87; p < 0.001). This study highlighted the impact of the multi-cultivar systems in strawberry production on the bioactive potential and the diversity of secondary metabolites obtained from strawberry agro-industrial by-products at a low cost.

In vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy—A medicinal plant

2017 ◽  
Vol 8 ◽  
pp. 31-39 ◽  
Author(s):  
Fatma Pehlivan Karakas ◽  
Arzu Ucar Turker ◽  
Alper Karakas ◽  
Vakhtang Mshvildadze ◽  
Andre Pichette ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Anti Inflammatory

scholarly journals Phenolic Compounds and Antioxidant Activity of Nepeta nuda subsp. Albiflora

2020 ◽  
Vol 79 ◽  
pp. 1-8
Author(s):  
İbrahim Teber ◽  
Ercan Bursal
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Plant Sample ◽  
Plant Biochemistry ◽  
Ethanol Extracts ◽  
Biochemical Analyses

Phenolic content and antioxidant activity of Nepeta nuda subsp. albiflora Boiss. were reported in this study. The ethanol and water extracts of Nepeta nuda subsp. albiflora were prepared and used for biochemical analyses. Antioxidant capacities of the extracts were evaluated by three different in vitro bioanalytical methods including a reducing antioxidant method and two radical scavenging antioxidant methods. The water and ethanol extracts of the plant sample were found to have effective antioxidant potentials. Phenolic content of Nepeta nuda subsp. albiflora was determined by high performance liquid chromatography (HPLC). Rosmarinic acid (182.0±4.5 µg/g), apigenin (84.5±57.6 µg/g), and quercetin (44.5±62.9 µg/g) were identified as major compounds in the ethanol extract of the plant sample. This study has a potential scientific base for further studies about Nepeta nuda subsp. albiflora related to plant biochemistry and plant based pharmacological industry.

W9, a medicinal plant from the pharmacopeia of the Eastern James Bay Cree, exhibits anti-diabetic activities in two mouse model of diabetes

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
HM Eid ◽  
M Ouchfoun ◽  
A Brault ◽  
D Vallerand ◽  
L Mussalam ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Mouse Model ◽  
James Bay ◽  
James Bay Cree
Sign in / Sign up

Export Citation Format

Share Document