scholarly journals The Variability of the Order Burkholderiales Representatives in the Healthcare Units

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Olga L. Voronina ◽  
Marina S. Kunda ◽  
Natalia N. Ryzhova ◽  
Ekaterina I. Aksenova ◽  
Andrey N. Semenov ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Lung Diseases ◽  
Burkholderia Cenocepacia ◽  
Bacteria Detection ◽  
Phylogenetic Position ◽  
Mlst Scheme ◽  
Chronic Lung Diseases ◽  
Sequence Types

Background and Aim. The order Burkholderiales became more abundant in the healthcare units since the late 1970s; it is especially dangerous for intensive care unit patients and patients with chronic lung diseases. The goal of this investigation was to reveal the real variability of the order Burkholderiales representatives and to estimate their phylogenetic relationships.Methods.16S rDNAand genes of theBurkholderia cenocepaciacomplex (Bcc) Multi Locus Sequence Typing (MLST) scheme were used for the bacteria detection.Results. A huge diversity of genome size and organization was revealed in the order Burkholderiales that may prove the adaptability of this taxon’s representatives. The following variability of the Burkholderiales in Russian healthcare units has been revealed: Burkholderiaceae (Burkholderia,Pandoraea, andLautropia), Alcaligenaceae (Achromobacter), and Comamonadaceae (Variovorax). TheBurkholderiagenus was the most diverse and was represented by 5 species and 16 sequence types (ST). ST709 and 728 were transmissible and often encountered in cystic fibrosis patients and in hospitals.A. xylosoxidanswas estimated by 15 genotypes. The strains of first and second ones were the most numerous.Conclusions. Phylogenetic position of the genusLautropiawith smaller genome is ambiguous. The Bcc MLST scheme is applicable for all Burkholderiales representatives for resolving the epidemiological problems.

scholarly journals Digging through the Obstruction: Insight into the Epithelial Cell Response to Respiratory Virus Infection in Patients with Cystic Fibrosis

2016 ◽  
Vol 90 (9) ◽  
pp. 4258-4261 ◽  
Author(s):  
Matthew R. Hendricks ◽  
Jennifer M. Bomberger
Keyword(s):  
Cystic Fibrosis ◽  
Lung Diseases ◽  
Respiratory Virus ◽  
Viral Infections ◽  
Molecular Diagnostic ◽  
Molecular Diagnostic Techniques ◽  
Virus Infections ◽  
Chronic Lung Diseases ◽  
Respiratory Viral Infections ◽  
Respiratory Virus Infections

Respiratory virus infections are common but generally self-limiting infections in healthy individuals. Although early clinical studies reported low detection rates, the development of molecular diagnostic techniques by PCR has led to an increased recognition that respiratory virus infections are associated with morbidity and acute exacerbations of chronic lung diseases, such as cystic fibrosis (CF). The airway epithelium is the first barrier encountered by respiratory viruses following inhalation and the primary site of respiratory viral replication. Here, we describe how the airway epithelial response to respiratory viral infections contributes to disease progression in patients with CF and other chronic lung diseases, including the role respiratory viral infections play in bacterial acquisition in the CF patient lung.

Analysis and HPLC fractionation of urine from patients with cystic fibrosis, chronic lung diseases and normal controls

1989 ◽  
Vol 22 (5) ◽  
pp. 377-383 ◽  
Author(s):  
Terese M. Guman-Wignot ◽  
Jay Kaufman ◽  
Douglas S. Holsclaw ◽  
Irvin R. Schmoyer ◽  
Jack Alhadeff
Keyword(s):  
Cystic Fibrosis ◽  
Lung Diseases ◽  
Chronic Lung Diseases ◽  
Hplc Fractionation ◽  
Normal Controls

scholarly journals Cystic Fibrosis: NHLBI Workshop on the Primary Prevention of Chronic Lung Diseases

2014 ◽  
Vol 11 (Supplement 3) ◽  
pp. S161-S168 ◽  
Author(s):  
Jessica E. Pittman ◽  
Garry Cutting ◽  
Stephanie D. Davis ◽  
Thomas Ferkol ◽  
Richard Boucher
Keyword(s):  
Cystic Fibrosis ◽  
Primary Prevention ◽  
Lung Diseases ◽  
Chronic Lung Diseases

scholarly journals Does cystic fibrosis constitute an advantage in COVID-19 infection?

2020 ◽  
Vol 46 (1) ◽  
Author(s):  
Valentino Bezzerri ◽  
Francesca Lucca ◽  
Sonia Volpi ◽  
Marco Cipolli
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Cystic Fibrosis ◽  
Lung Diseases ◽  
Respiratory Viruses ◽  
Chronic Obstructive ◽  
Veneto Region ◽  
Obstructive Pulmonary Disease ◽  
Chronic Lung Diseases ◽  
Italian Regions ◽  
Pulmonary Impairment

Abstract The Veneto region is one of the most affected Italian regions by COVID-19. Chronic lung diseases, such as chronic obstructive pulmonary disease (COPD), may constitute a risk factor in COVID-19. Moreover, respiratory viruses were generally associated with severe pulmonary impairment in cystic fibrosis (CF). We would have therefore expected numerous cases of severe COVID-19 among the CF population. Surprisingly, we found that CF patients were significantly protected against infection by SARS-CoV-2. We discussed this aspect formulating some reasonable theories.

scholarly journals Seroprevalence ofToxoplasma gondiiInfection in Patients of Intensive Care Unit in China: A Hospital Based Study

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Yong-Biao Zhang ◽  
Wei Cong ◽  
Zhi-Tao Li ◽  
Xiao-Gang Bi ◽  
Ying Xian ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Lung Diseases ◽  
Latent Infection ◽  
Genetic Characterization ◽  
Kidney Diseases ◽  
Brain Diseases ◽  
Age Group ◽  
Igg Antibodies ◽  
Icu Patients

The objective of this study was to estimate the seroprevalence ofToxoplasma gondiiinfection in 394 patients of intensive care unit (ICU) in a hospital between April 2010 and March 2012 and analyze the association betweenT. gondiiinfection and ICU patients according to the species of disease.Toxoplasmaserology was evaluated by ELISA method using a commercially available kit. Data of patients were obtained from the patients, informants, and medical examination records. Seventy-four (18.78%) of 394 patients were positive for anti-T. gondiiIgG antibodies demonstrating latent infection.Of these, the highestT. gondiiseroprevalence was found in the age group of 31–45 years (27.45%), and the lowest was found in the age group of <30 years (12.5%). In addition, females (21.6%) had a higher seroprevalence than males (18.36%). With respect to the species of disease, the patients with kidney diseases (57.14%), lung diseases (27.84%), and brain diseases (24%) had highT. gondiiseroprevalence. The present study represents the first survey ofT. gondiiseroprevalence in ICU patients in China, revealing an 18.78% seropositivity. Considering the particularities of ICU patients, molecular identification, genetic characterization, and diagnosis ofT. gondiishould be considered in future study.

scholarly journals Lack of Association between Hypermutation and Antibiotic Resistance Development in Pseudomonas aeruginosa Isolates from Intensive Care Unit Patients

2004 ◽  
Vol 48 (9) ◽  
pp. 3573-3575 ◽  
Author(s):  
Olivia Gutiérrez ◽  
Carlos Juan ◽  
José L. Pérez ◽  
Antonio Oliver
Keyword(s):  
Cystic Fibrosis ◽  
Intensive Care Unit ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Intensive Care ◽  
Large Collection ◽  
Resistance Development ◽  
Icu Patients

ABSTRACT Hypermutation is a common feature of Pseudomonas aeruginosa isolates from chronically infected cystic fibrosis patients that is linked with antibiotic resistance development. In this work, using a large collection of sequential P. aeruginosa isolates from ICU patients, we found that despite the fact that mutational antibiotic resistance development is a frequent outcome, the prevalence of hypermutable strains is low (found in isolates from only 1 of 103 patients) and there is no evidence of coselection of the hypermutable and antibiotic resistance phenotypes.

scholarly journals Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Isolated in the Intensive Care Unit of the Largest Tertiary Hospital in Bangladesh

2021 ◽  
Vol 11 ◽  
Author(s):  
Takashi Okanda ◽  
Anwarul Haque ◽  
Takuro Koshikawa ◽  
Amirul Islam ◽  
Qumrul Huda ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Drug Resistance ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Tertiary Hospital ◽  
Antimicrobial Treatment ◽  
Antimicrobial Drug ◽  
New Delhi ◽  
Antimicrobial Drug Resistance ◽  
Sequence Types

For addressing the issue of antimicrobial drug resistance in developing countries, it is important to investigate the characteristics of carbapenemase-producing organisms. We aimed to genetically characterize a carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated in the intensive care unit of a tertiary hospital in Bangladesh. The number of CPKP isolates were 43/145 (30%), of which pandrug-resistant (PDR) strains were 14%. These carbapenemases were New Delhi metallo-beta-lactamase (NDM)-1 (53%), NDM-5 (14%), oxacillinase (OXA)-181 (12%), OXA-232 (10%), NDM-5 + OXA-181 (5%), and NDM-5 + OXA-232 (2%). Many CPKP isolates harbored a variety of resistance genes, and the prevalence of 16S rRNA methyltransferase was particularly high (91%). The 43 CPKP isolates were classified into 14 different sequence types (STs), and the common STs were ST34 (26%), ST147 (16%), ST11 (9%), ST14 (9%), ST25 (7%), and ST231 (7%). In this study, PDR strains were of three types, ST147, ST231, and ST14, and their PDR rates were 57, 33, and 25%, respectively. The spread of the antimicrobial drug resistance of CPKP in Bangladesh was identified. In particular, the emergence of PDR is problem, and there may be its spread as a superbug of antimicrobial treatment.

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