High 15-F2t-Isoprostane Levels in Patients with a Previous History of Nonmelanoma Skin Cancer: The Effects of Supplementary Antioxidant Therapy

Background. Phase I of this study was aimed at comparing the profiles of oxidative stress biomarkers in patients with history of nonmelanoma skin cancer (NMSC), previously treated with surgery, to the healthy subjects. Phase II aimed to evaluate the effects of supplementary antioxidant therapy on the levels of biomarkers in the case group.Materials and Methods. In Phase I, oxidative stress biomarkers were measured in blood samples obtained from 24 healthy subjects and 60 patients with history of NMSC previously treated with surgery. In Phase II, the 60 patients with history of NMSC were randomized into two subgroups, one receiving placebo (n=34) and the other (n=26) receiving vitamin C, vitamin E, and zinc supplementation for 8 weeks, followed by reevaluation of biomarkers.Results. In Phase I, patients with history of NMSC showed increased plasma concentrations of all biomarkers, but only 15-F2t-isoprostane was significantly higher than in the healthy subjects. Risk of NMSC increased by 4% for each additional 1 pg/mL increase in 15-F2t-isoprostane. In Phase II, supplementation did not significantly reduce levels of oxidative stress biomarkers.Conclusion. Patients with history of NMSC had significantly high 15-F2t-isoprostane plasma levels; supplementation did not result in significant reduction of oxidative stress biomarkers. This trial was registered with ClinicalTrials.gov (IDNCT02248584).

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Seasonal changes in oxidative stress biomarkers of the snail Viviparus acerosus from the Velika Morava River, Serbia

Archives of Biological Sciences ◽

10.2298/abs1203953d ◽

2012 ◽

Vol 64 (3) ◽

pp. 953-962 ◽

Cited By ~ 5

Author(s):

Svetlana Despotovic ◽

Branka Perendija ◽

Jelena Gavric ◽

Slavica Borkovic-Mitic ◽

M.M. Paunovic ◽

...

Keyword(s):

Oxidative Stress ◽

Phase Ii ◽

Seasonal Changes ◽

Important Variable ◽

Whole Body ◽

Glutathione S Transferase ◽

Total Glutathione ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Morava River

The river snail (Viviparus acerosus) from the Velika Morava River, Serbia was chosen in our study in order to determine seasonal changes in oxidative stress biomarkers between July (summer) and September (autumn). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione-S-transferase (GST), as well as the concentration of total glutathione (GSH), were examined in the whole body of the river snails. The obtained results show significantly higher activities of CAT, GSH-Px, GR and biotransformation phase II enzyme GST in September compared to July, while the GSH concentration was lower. There was no general trend in the seasonal changes in the activity of SOD. The presented data show that animals in their natural environment are exposed to constant fluctuations of environmental conditions that could cause antioxidants to exhibit seasonal variations. This fact should be considered as an important variable in the interpretation of results in biomonitoring studies.

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Body Mass Index and Age-Related Changes of ROS Production and Oxidative Stress Biomarkers in Healthy Subjects

10.20455/ros.2019.841 ◽

2019 ◽

Author(s):

Alessandra Vezzoli ◽

Maristella Gussoni ◽

Michela Montorsi ◽

Simona Mrakic-Sposta

Keyword(s):

Oxidative Stress ◽

Body Mass Index ◽

Body Mass ◽

Healthy Subjects ◽

Ros Production ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Age Related ◽

And Oxidative Stress ◽

Age Related Changes

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OXIDATIVE STRESS BIOMARKERS IN EXPIRATED AIR OF HEALTHY HUMANS AND PROMISING TECHNIQUES OF THEIR ANALYSIS DURING SPACE FLIGHT

Aerospace and Environmental Medicine ◽

10.21687/0233-528x-2020-54-3-5-21 ◽

2020 ◽

pp. 5-21

Author(s):

L.N. Mukhamedieva ◽

◽

D.S. Tsarkov ◽

D.S. Ozerov ◽

G.Yu. Grigoriev ◽

...

Keyword(s):

Oxidative Stress ◽

Space Flight ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Healthy Humans

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Association between Vitamin D Status, Oxidative Stress Biomarkers and Viral Load in Human Immunodeficiency Virus Type 1 Infection

Current HIV Research ◽

10.2174/1570162x15666171005170227 ◽

2017 ◽

Vol 15 (5) ◽

Cited By ~ 4

Author(s):

Tamires Flauzino ◽

Andrea N.C. Simao ◽

Elaine R.D. de Almeida ◽

Helena K. Morimoto ◽

Sayonara R. Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Human Immunodeficiency Virus ◽

Viral Load ◽

Human Immunodeficiency Virus Type ◽

Vitamin D Status ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Immunodeficiency Virus

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Oxidative Stress Biomarkers in Urine of Metal Carpentry Workers Can Be Diagnostic for Occupational Exposure to Low Level of Welding Fumes from Associated Metals

Cancers ◽

10.3390/cancers13133167 ◽

2021 ◽

Vol 13 (13) ◽

pp. 3167

Author(s):

Flavia Buonaurio ◽

Maria Luisa Astolfi ◽

Daniela Pigini ◽

Giovanna Tranfo ◽

Silvia Canepari ◽

...

Keyword(s):

Oxidative Stress ◽

Occupational Exposure ◽

Oxidation Products ◽

Control Group ◽

Welding Fumes ◽

Oxidative Stress Biomarkers ◽

Limit Values ◽

Stress Biomarkers ◽

Specificity And Sensitivity ◽

The Impact

Urinary concentrations of 16 different exposure biomarkers to metals were determined at the beginning and at the end of a working shift on a group of workers in the metal carpentry industry. Five different oxidative stress biomarkers were also measured, such as the oxidation products of RNA and DNA metabolized and excreted in the urine. The results of workers exposed to metals were compared to those of a control group. The metal concentrations found in these workers were well below the occupational exposure limit values and exceeded the mean concentrations of the same metals in the urine of the control group by a factor of four at maximum. Barium (Ba), mercury (Hg), lead (Pb) and strontium (Sr) were correlated with the RNA oxidative stress biomarker, 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), which was found able to discriminate exposed workers from controls with a high level of specificity and sensitivity. The power of this early diagnostic technique was assessed by means of the ROC curve. Ba, rubidium (Rb), Sr, tellurium (Te), and vanadium (V) were correlated with the level of the protein oxidation biomarker 3-Nitrotyrosine (3-NO2Tyr), and Ba, beryllium (Be), copper (Cu), and Rb with 5-methylcytidine (5-MeCyt), an epigenetic marker of RNA damage. These effect biomarkers can help in identifying those workers that can be defined as “occupationally exposed” even at low exposure levels, and they can provide information about the impact that such doses have on their health.

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Antioxidants, lysosomes and elements status during the life cycle of sea trout Salmo trutta m. trutta L.

Scientific Reports ◽

10.1038/s41598-021-85127-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Natalia Kurhaluk ◽

Halyna Tkachenko

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Muscle Tissue ◽

Salmo Trutta ◽

Protein Carbonyl ◽

Oxidative Modification ◽

Oxidative Stress Biomarkers ◽

Development Stages ◽

Stress Biomarkers ◽

Element Contents

AbstractThe aim of our study was to elucidate the effects of both development stages (parr, smolt, adult, spawner), and kelt as a survival form and sex (male, female) on the functional stability of the lysosomal complex, biomarkers of oxidative stress, and element contents in the muscle tissue of the sea trout (Salmo trutta m. trutta L.) sampled in the Pomerania region (northern Poland). We have evaluated the maximal activities of lysosomal enzymes (alanyl aminopeptidase, leucyl aminopeptidase, β-N-acetylglucosaminidase, and acid phosphatase), lipid peroxidation level, and protein carbonyl derivatives as indices of muscle tissue degradation. The relationship between lysosomal activity and oxidative stress biomarkers estimated by the lipid peroxidation level and protein carbonyl derivatives was also assessed, as well as the relationships between element levels and oxidative stress biomarkers. Trends of the main effects (i.e., the development stages and sex alone, the interaction of the sex and development stage simultaneously) on oxidative stress biomarkers, lysosomal functioning, and element contents in the muscle tissue were evaluated. The study has shown sex-related relationships between the pro- and antioxidant balance and the tissue type in the adult stage as well as modifications in the lysosomal functioning induced by long-term environmental stress associated with changing the habitats from freshwater to seawater and intense migrations. The highest level of toxic products generated in oxidative reactions and oxidative modification of proteins was noted in both the spawner stage and the kelt form. The holistic model of analysis of all parameters of antioxidant defense in all development stages and sex demonstrated the following dependencies for the level of lipid peroxidation, oxidative modification of proteins, lysosomal activities, and element contents: TBARS > OMP KD > OMP AD > TAC, AcP > NAG > LAP > AAP and Cu > Fe > Ca > Mn > Zn > Mg, respectively.

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