scholarly journals Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557)

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Mengrui Yang ◽  
Min Wang ◽  
Jian Zhou ◽  
Yinqing Song ◽  
Tongtong Wang
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Mass Fraction ◽  
High Performance ◽  
Uncertainty Assessment ◽  
Stability Study ◽  
Property Value ◽  
Routine Measurement ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Comparability Of Results

Prior to preparation of CRM candidate of chloramphenicol in methanol with a concentration of 100 mg/L, two independent methods including mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) were employed to precisely measure the mass fraction of pure chloramphenicol materials. The mass fraction was assigned to be 99.8% with uncertainty of 0.3%. Homogeneity testing and stability study of chloramphenicol in methanol were examined by using high performance liquid chromatography. Additionally, the uncertainties originating from the process of CRM development were comprehensively evaluated. The experimental results indicate that the property value of this CRM is homogeneous and stable at 4°C for at least six months. The new CRM (GBW(E)082557) can be applicable to calibration of instrument and assurance of accuracy and comparability of results in routine measurement.

QUALITY ASSESSMENT AND QUANTIFICATION OF GENISTEIN IN DIETARY SUPPLEMENTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, QUANTITATIVE NUCLEAR MAGNETIC RESONANCE, AND TWO-DIMENSIONAL DIFFUSION ORDERED SPECTROSCOPY 1H

2020 ◽  
pp. 132-142
Author(s):  
VLACHOU IOANNA ◽  
PAPASOTIRIOU IOANNIS
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Dietary Supplements ◽  
Active Ingredient ◽  
High Performance ◽  
Two Dimensional ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Dosy Nmr

Objective: The aim of the present study is to analyze four herbal dietary supplements containing genistein by liquid chromatography, quantitative nuclear magnetic resonance (qNMR), and diffusion ordered spectroscopy (DOSY) 1H NMR. Methods: Quantification of the active ingredient, genistein, is carried out by high-performance liquid chromatography (HPLC) and qNMR. Two-dimensional (2D) DOSY NMR also allows the qualitative analysis of the samples with the detection of active ingredient and excipients present in the formulations. Results: The validated HPLC and qNMR methods showed that all four supplements contain genistein in different amounts, and 2D DOSY NMR provides a clear image of all ingredients in the formulations. Conclusion: The use of the three techniques provides detailed information on each product and its contents, and all of them are currently used for the quality control of natural supplements by our laboratory.

High performance liquid chromatography-quantitative nuclear magnetic resonance (HPLC-qNMR) with a two-signal suppression method for purity assessment of avermectin B1a

2016 ◽  
Vol 8 (22) ◽  
pp. 4482-4486 ◽  
Author(s):  
Ting Huang ◽  
Wei Zhang ◽  
Xinhua Dai ◽  
Na Li ◽  
Liang Huang ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Magnetic Resonance ◽  
High Performance ◽  
Signal Suppression ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Suppression Method ◽  
High Separation

HPLC-qNMR is a technology with great potential, which combines the high separation capability of HPLC and the high qualitative/quantitative analysis capability of NMR.

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