scholarly journals Lipid Reconstitution-Enabled Formation of Gold Nanoparticle Clusters for Mimetic Cellular Membrane

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jiyoung Nam ◽  
Yong-Tae Kim ◽  
Aeyeon Kang ◽  
Kook-Han Kim ◽  
KyoRee Lee ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Cellular Uptake ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Cellular Membrane ◽  
Phospholipid Bilayers ◽  
Head Group ◽  
Oligomeric State ◽  
Nanoparticle Clusters

Gold nanoparticles (AuNPs) encapsulated within reconstituted phospholipid bilayers have been utilized in various bioapplications due to their improved cellular uptake without compromising their advantages. Studies have proved that clustering AuNPs can enhance the efficacy of theranostic effects, but controllable aggregation or oligomerization of AuNPs within lipid membranes is still challenging. Here, we successfully demonstrate the formation of gold nanoparticle clusters (AuCLs), supported by reconstituted phospholipid bilayers with appropriate sizes for facilitating cellular uptake. Modulation of the lipid membrane curvatures influences not only the stability of the oligomeric state of the AuCLs, but also the rate of cellular uptake. Dynamic light scattering (DLS) data showed that 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), with its relatively small head group, is crucial for establishing an effective membrane curvature to encapsulate the AuCLs. The construction of phospholipid bilayers surrounding AuCLs was confirmed by analyzing the secondary structure of M2 proteins incorporated in the lipid membrane surrounding the AuCLs. When AuCLs were incubated with cells, accumulated clusters were found inside the cells without the lipids being removed or exchanged with the cellular membrane. We expect that our approach of clustering gold nanoparticles within lipid membranes can be further developed to design a versatile nanoplatform.

scholarly journals Rapid evaluation of gold nanoparticle–lipid membrane interactions using a lipid/polydiacetylene vesicle sensor

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3049-3055
Author(s):  
Congcong Gu ◽  
Yingying Geng ◽  
Feng Zheng ◽  
Vincent M. Rotello
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Rapid Evaluation ◽  
Membrane Interactions

Lipid/polydiacetylene vesicles serve as a sensor to rapidly measure the interactions between gold nanoparticles and lipid membranes. A colorimetric signal is generated upon membrane disturbance caused by gold nanoparticles.

The plasmonic properties of gold nanoparticle clusters formed via applying an AC electric field

Soft Matter ◽  
2018 ◽  
Vol 14 (17) ◽  
pp. 3372-3377 ◽  
Author(s):  
K. Watanabe ◽  
E. Tanaka ◽  
H. Ishii ◽  
D. Nagao
Keyword(s):  
Gold Nanoparticles ◽  
Electric Field ◽  
Gold Nanoparticle ◽  
Ac Electric Field ◽  
Nanoparticle Clusters

Plasmonic properties of gold nanoparticles in suspension were correlated to the clustering states of the particles formed by the application of an AC electric field.

Penetration of Lipid Membranes by Gold Nanoparticles: Insights into Cellular Uptake, Cytotoxicity, and Their Relationship

ACS Nano ◽  
2010 ◽  
Vol 4 (9) ◽  
pp. 5421-5429 ◽  
Author(s):  
Jiaqi Lin ◽  
Hongwu Zhang ◽  
Zhen Chen ◽  
Yonggang Zheng
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Lipid Membranes

scholarly journals Specific and nondisruptive interaction of guanidium-functionalized gold nanoparticles with neutral phospholipid bilayers

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Lucía Morillas-Becerril ◽  
Sebastian Franco-Ulloa ◽  
Ilaria Fortunati ◽  
Roberto Marotta ◽  
Xiaohuan Sun ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Lipid Membranes ◽  
Phospholipid Bilayers ◽  
Phosphatidylcholine Liposomes ◽  
Functionalized Gold Nanoparticles ◽  
Naturally Occurring ◽  
Bonding Interaction ◽  
Biological Entities ◽  
Membranotropic Properties ◽  
Positively Charged

AbstractUnderstanding and controlling the interaction between nanoparticles and biological entities is fundamental to the development of nanomedicine applications. In particular, the possibility to realize nanoparticles capable of directly targeting neutral lipid membranes would be advantageous to numerous applications aiming at delivering nanoparticles and their cargos into cells and biological vesicles. Here, we use experimental and computational methodologies to analyze the interaction between liposomes and gold nanoparticles (AuNPs) featuring cationic headgroups in their protecting monolayer. We find that in contrast to nanoparticles decorated with other positively charged headgroups, guanidinium-coated AuNPs can bind to neutral phosphatidylcholine liposomes, inducing nondisruptive membrane permeabilization. Atomistic molecular simulations reveal that this ability is due to the multivalent H-bonding interaction between the phosphate residues of the liposome’s phospholipids and the guanidinium groups. Our results demonstrate that the peculiar properties of arginine magic, an effect responsible for the membranotropic properties of some naturally occurring peptides, are also displayed by guanidinium-bearing functionalized AuNPs.

DNA templated self-assembly of gold nanoparticle clusters in the colorimetric detection of plant viral DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11773-11785 ◽  
Author(s):  
G. Dharanivasan ◽  
S. U. Mohammed Riyaz ◽  
D. Michael Immanuel Jesse ◽  
T. Raja Muthuramalingam ◽  
G. Rajendran ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Oligonucleotide Probe ◽  
Colorimetric Detection ◽  
Viral Dna ◽  
Nanoparticle Clusters

The DNA templated self-assembly of gold nanoparticles clustered in different configurations (nn = 2–∞) was investigated in the colorimetric detection of ToLCNDV DNA using a gold nanoparticle conjugated bifunctional oligonucleotide probe.

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

scholarly journals Electrochemical Properties of Lipid Membranes Self-Assembled from Bicelles

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek
Keyword(s):  
Electrochemical Properties ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Membrane Resistance ◽  
Electrochemical Characteristics ◽  
Freeze Thaw ◽  
Lipid Films ◽  
Supported Lipid Membranes

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.

scholarly journals Interactions of Linear Analogues of Battacin with Negatively Charged Lipid Membranes

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 192
Author(s):  
Kinga Burdach ◽  
Dagmara Tymecka ◽  
Aneta Urban ◽  
Robert Lasek ◽  
Dariusz Bartosik ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Liquid Crystalline ◽  
Lipid Membrane ◽  
Attenuated Total Reflection ◽  
Gram Positive ◽  
Insertion Depth ◽  
Force Microscopy ◽  
Acyl Chains ◽  
Hydrophobic Portion ◽  
Membrane Fluidization

The increasing resistance of bacteria to available antibiotics has stimulated the search for new antimicrobial compounds with less specific mechanisms of action. These include the ability to disrupt the structure of the cell membrane, which in turn leads to its damage. In this context, amphiphilic lipopeptides belong to the class of the compounds which may fulfill this requirement. In this paper, we describe two linear analogues of battacin with modified acyl chains to tune the balance between the hydrophilic and hydrophobic portion of lipopeptides. We demonstrate that both compounds display antimicrobial activity with the lowest values of minimum inhibitory concentrations found for Gram-positive pathogens. Therefore, their mechanism of action was evaluated on a molecular level using model lipid films mimicking the membrane of Gram-positive bacteria. The surface pressure measurements revealed that both lipopeptides show ability to bind and incorporate into the lipid monolayers, resulting in decreased ordering of lipids and membrane fluidization. Atomic force microscopy (AFM) imaging demonstrated that the exposure of the model bilayers to lipopeptides leads to a transition from the ordered gel phase to disordered liquid crystalline phase. This observation was confirmed by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) results, which revealed that lipopeptide action causes a substantial increase in the average tilt angle of lipid acyl chains with respect to the surface normal to compensate for lipopeptide insertion into the membrane. Moreover, the peptide moieties in both molecules do not adopt any well-defined secondary structure upon binding with the lipid membrane. It was also observed that a small difference in the structure of a lipophilic chain, altering the balance between hydrophobic and hydrophilic portion of the molecules, results in different insertion depth of the active compounds.

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