scholarly journals Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Adventitious Shoots ◽  
Conservation Strategy ◽  
Soil Conditions ◽  
Natural Soil ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Medicinal Tree ◽  
Couroupita Guianensis

The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of Couroupita guianensis (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds’ induction and their growth were observed with α-Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of C. guianensis were rooted and shoots were induced when pretreated with 400 mg L−1 NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg L−1 indole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg L−1 indole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of C. guianensis through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals Vegetative propagation of Garcinia lucida Vesque (Clusiaceae) using leafy stem cuttings and grafting

Afrika Focus ◽  
2017 ◽  
Vol 27 (3) ◽  
Author(s):  
Bertin Takoutsing ◽  
Alain Tsobeng ◽  
Zacharie Tchoundjeu ◽  
Ann Degrande ◽  
Ebenazar Asaah
Keyword(s):  
Acetic Acid ◽  
Leaf Area ◽  
Vegetative Propagation ◽  
Naphthalene Acetic Acid ◽  
High Porosity ◽  
Stem Cuttings ◽  
Forest Zone ◽  
Indole Butyric Acid ◽  
Humid Forest ◽  
Humid Forest Zone

Garcinia lucida Vesque (Clusiaceae) is a tree species that is highly valued for its medicinal properties by rural households in the humid forest zone of Cameroon. However, the unsustainable exploitation of the species threatens its long-term regeneration. This study focuses on its vegetative propagation via stem cuttings in non-mist propagators and through grafting. The study tests the effects of three rooting media (sand, sawdust, sand + sawdust (1/1); three leaf sizes (0,25 and 50 cm2); and three types of hormone [indole butyric acid (IBA), indole-3-acetic acid (IAA) and naphthalene acetic acid (NAA)], applied as a single dose. Furthermore, three grafting techniques (cleft, side tongue and whip-and-tongue grafting) were tested. All experiments were designed as completely randomized blocks with three replicates. Results showed that cuttings require a medium that has low water holding capacity and high porosity, and a leaf area of 50 cm2 and NAA treatment; grafting success was affected by the technique used, with top cleft grafting yielding a 100% success rate. From this preliminary study, it is concluded that G. lucida is amenable to vegetative propagation by cuttings and grafting. Key words: tree domestication, leaf area, non-mist propagator, rooting hormone, rooting medium, under-utilised species 

scholarly journals In vitro culture of rose species (Rosa spp.) via axillary bud growth

2007 ◽  
pp. 1-13
Author(s):  
Zosimo de la Rosa ◽  
Marilyn Belarmino
Keyword(s):  
Acetic Acid ◽  
Adventitious Shoots ◽  
Axillary Buds ◽  
Plant Production ◽  
Axillary Bud ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Single Shoot

The utilization of nodal stem cuttings containing dormant axillary buds as explants for plant production of two rose species; Rosa chinensis cv.‘Old Blush’ and R. centifolia cv. ‘Petite de Hollande’ was demonstrated in this study. This propagation technique required the breaking of dormant axillary buds by aseptically culturing them in agarsolidified Woody Plant Medium (WPM) added with 0.5, 1.0, and 2.0 mg l-1 of 6benzylaminopurine (BAP) or, a combination of 2.0 mg l-1 BAP and 0.01 mg l-1 naphthalene acetic acid (NAA). Production of multiple adventitious shoots from one nodal stem explant was obtained after three months of culture in medium supplemented with 1.0 or 2.0 mg l-1 BAP. Four types of plant morphology; single shoot (type 1), multiple shoots with normal leaves (type 2), cluster of tiny shoots with curly leaves (type 3), and single shoot with callus at the base (type 4) were observed from the axillary bud-derived plantlets. The rooting of plantlets was induced in WPM containing 0.25 to 1.0 mg l-1 of indole-butyric acid (IBA) or, 2.0 mg l-1 of indole-3-acetic acid (IAA).

scholarly journals VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

2019 ◽  
Vol 32 (4) ◽  
pp. 858-866
Author(s):  
GABRIELA TEODORO ROCHA ◽  
AYURE GOMES DA SILVA ◽  
JULIANA BEZERRA MARTINS ◽  
NEI PEIXOTO ◽  
FABRICIO RODRIGUES
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Spondias Tuberosa ◽  
Completely Randomized Design ◽  
Low Efficiency ◽  
Indole 3 Acetic Acid ◽  
Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

scholarly journals Factors Affecting Vegetative Propagation of Wintersweet (Chimonanthus praecox) by Softwood Cuttings

HortScience ◽  
2020 ◽  
Vol 55 (11) ◽  
pp. 1853-1860
Author(s):  
Huan Hu ◽  
Nan Chai ◽  
Haoxiang Zhu ◽  
Rui Li ◽  
Renwei Huang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Design Method ◽  
Orthogonal Design ◽  
Naphthalene Acetic Acid ◽  
Hormone Concentration ◽  
Range Analysis ◽  
Indole Butyric Acid ◽  
Factors Affecting ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox) is one of the most popular winter-blooming species. Effective vegetative propagation is necessary for commercial usage and protection of wintersweet. In the current study, the four factors, namely hormone type (A), hormone concentration (B), soaking duration (C), and medium (D), were assessed using an L16 (44) orthogonal test design. The hormone types include ABT (A1), α-naphthalene acetic acid (NAA) (A2), indole butyric acid (IBA) (A3), and indole-3-acetic acid (IAA) (A4); the hormone concentrations include 100 mg·L−1 (B1), 500 mg·L−1 (B2), 1000 mg·L−1 (B3), and 1500 mg·L−1 (B4); the soaking durations include 5 seconds (C1), 5 minutes (C2), 30 minutes (C3), and 3 hours (C4); and the mediums include perlite: peat in the ratios 1:0 (D1), 2:1 (D2), 1:1 (D3), and 1:2 (D4). The results showed that hormone and proper medium could significantly improve the cutting survival, rooting, and sprouting, whereas poor factor combinations, especially high hormone concentrations combined with long soaking durations may be threatened to the cuttings and rooting. In actual experiments, we successfully obtained an excellent rooting percentage (62.22%) of wintersweet from treatment No. 5 (A2B1C2D3), which is perlite and peat (1:1) as the medium and soaking the cuttings in 100 mg·L−1 NAA for 5 minutes as the hormone treatment. This combination can already meet the requirements for commercial production. A range analysis showed that the medium and hormone concentration were the most important factors affecting the cutting of wintersweet. An analysis of variance also showed that the medium and hormone concentration can significantly or extremely significantly affect most cutting indicators. Moreover, our results revealed that an orthogonal design method is an effective tool for establishing an improved technique for cutting propagation.

scholarly journals Somatic embryogenesis and plant regeneration in purple food yam (Dioscorea alata L.)

2008 ◽  
pp. 22-33 ◽  
Author(s):  
Marilyn Belarmino ◽  
Jocelyn Gonzales
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Adventitious Shoots ◽  
Pantothenic Acid ◽  
Naphthalene Acetic Acid ◽  
Dioscorea Alata ◽  
Ms Medium ◽  
Leaf Petiole

A study was conducted to establish a reliable procedure for somatic embryogenesis and plant regeneration from callus cultures of purple food yam (Dioscorea alata L.). The procedure involved three steps; (1) culture of nodal stem segments from greenhousegrown plants to generate in vitro plantlets; (2) induction of callus from the leaf, petiole and nodal stem tissues; and (3) initiation of somatic embryo from callus. Results showed that the agar-solidified Murashige and Skoog (MS) medium containing 30 gl-1 sugar, 0.1 gl-1 α-cysteine , 10 mgl-1 calcium pantothenic acid, 2.0 mgl-1 asparagine, 2.0 mgl-1 arginine, 80.0 mgl-1 adenine sulfate (AdSO4) and 0.1 mgl-1 naphthalene acetic acid (NAA) effectively broke dormancy of lateral buds of nodal stem cultures from both ‘VU-2’ and ‘Kinampay‘ varieties. Production of multiple adventitious shoots occurred after transfer of in vitro nodal pieces to the same medium added with 1.0 mgl-1 benzylamino purine (BAP) or, MSA medium. Callus was effectively induced from the vegetative tissues in MS medium added with 1.0 mgl-1 2,4-Dichlorophenoxy acetic acid (2,4-D) or, with picloram. Among the three types of explants, the nodal stem was the most suitable which produced purplish nodular embryogenic callus. A higher percentage of nodal stem-derived calli produced globular embryos in MS medium containing 1.0 mgl-1 2,4-D and 0.5 mgl-1 BAP, or in 1.0 mgl-1 picloram and 0.5 mgl-1 BAP than, in the plant growth regulator-free medium (control). The maturation of embryos was facilitated by one-month culture in MS medium containing 0.1 mgl-1 ABA and 100 mgl-1 glutamine. This step improved the germination of somatic embryos in one-half strength PGR-free MS medium containing 100 mgl-1 glutamine (regeneration medium). All somatic embryoderived plantlets were morphologically normal and established well in soil.

scholarly journals Effect of naphthalene acetic acid on sprouting and rooting of stem cutting in Mussaenda species

2019 ◽  
Vol 30 (1) ◽  
pp. 48-54
Author(s):  
MHA Rashid ◽  
A Nahar
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Block Design ◽  
Stem Cutting ◽  
Naphthalene Acetic Acid ◽  
Combined Effects ◽  
Stem Cuttings ◽  
Treatment Combination ◽  
Red Flag ◽  
Number Of Leaves

Mussaendas are increasingly popular for the showy colour they provide during much of the year in garden landscapes. However, root formation in stem cuttings is a major challenge for cultivation of Mussaenda species. Therefore, the experiment was carried out at the Landscaping section of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh during the period from August to November 2018 to study the effect of phytohormone naphthalene acetic acid (NAA) on sprouting and rooting of stem cuttings in Mussaenda species. The two-factor experiment consisted of three Mussaenda species viz., Mussaenda frondosa (White flag bush), Mussaenda philippica (Pink flag bush) and Mussaenda erythrophylla (Red flag bush), and five concentrations of NAA viz., 0 (control), 0.1%, 0.2%, 0.3% and 0.4%. The experiment was laid out in randomised complete block design (RCBD) with three replications. The results revealed that all the traits related to sprouting and rooting potential of stem cuttings were significantly influenced by combined effects of Mussaenda species and various NAA concentrations. The maximum sprouted bud per cutting (3.57), highest number of roots per cutting (5.88) and the longest root length (4.32 cm) were recorded from M. philippica except number of leaves per stem cutting (4.42) in M. erythrophylla. The increasing concentrations of NAA application responded positively on sprouting and rooting characters in all three Mussaenda species used for this experiment. The best results obtained from the treatment where NAA was applied at the concentration of 0.3% with 3.92 sprouted buds per cutting, 4.77 leaves per cutting, 7.15 roots per cutting and 5.26 cm length of roots. The treatment combination of M. philippica (Pink flag bush) and 0.3% NAA, followed by NAA at 03% with M. erythrophylla (Red flag bush) was found to be best in terms of sprouting and rooting of stem cuttings in Mussaenda. Progressive Agriculture 30 (1): 48-54, 2019

scholarly journals Rooting Efficiency of Amur Maple Seedless Selections Produced by Mutagenesis1

2020 ◽  
Vol 38 (2) ◽  
pp. 37-43
Author(s):  
Andrzej K. Noyszewski ◽  
Alan G. Smith
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Customer Choice ◽  
Asexual Propagation ◽  
Propagation Methods ◽  
Noxious Weed ◽  
Acer Ginnala

Abstract Amur maple (Acer ginnala Maxim.) is a widely planted small tree with attractive red fall foliage. In many states, it is classified as a noxious weed and regulations restrict propagation and sale, limiting customer choice. Stem cuttings are commonly used as a means of asexual propagation of Amur maple, with softwood cuttings being the easiest to root. Here we report adventitious rooting efficiency of seedless Amur maple selections using four indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and four naphthalene acetic acid (NAA; 100, 500, 1,500, 5,000 ppm) treatments and a no hormone control. Overall, cuttings treated with 5,000 or 10,000 ppm IBA and 5,000 ppm NAA resulted in the highest percent rooting (PR), mean number of roots (MNR), and mean length of the longest root (MRL) across all seedless selections. Seedless selections SW-30-14 and SW-30-130 had the highest PR, 66% and 67%, respectively, and will perform well in commercial propagation. This level of rooting was greater than that of the cultivar ‘Bailey Compact' that had 46% PR, and the seedless selection SW-30-159 that had the lowest PR (10.3%), MNR (0.3), and MRL (0.2 in) averaged across all treatments. Cuttings from 4 of the 5 seedless selections treated with 1,500 ppm NAA had the greatest PR, whereas ‘Bailey Compact' and SW-30-159 had the greatest PR with 5,000 ppm NAA. Results indicate that rooting efficiency was impacted by mutagenesis in some selections, which may require optimization of propagation methods for those selections. Index words: adventitious rooting, auxin, vegetative propagation, sterile, non-native invasive. Species used in this study: Amur maple (Acer ginnala Maxim.). Chemicals used in this study: indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and naphthalene acetic acid (NAA; 100 500, 1,500 5,000 ppm).

scholarly journals Effects of Substrate and Exogenous Auxin on the Adventitious Rooting of Dianthus caryophyllus L.

HortScience ◽  
2020 ◽  
Vol 55 (2) ◽  
pp. 170-173
Author(s):  
Liang Zheng ◽  
Zibin Xiao ◽  
Weitang Song
Keyword(s):  
Acetic Acid ◽  
Physicochemical Properties ◽  
Root Formation ◽  
Dianthus Caryophyllus ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Experimental Plant ◽  
Stem Cuttings ◽  
Exogenous Auxin ◽  
Auxin Concentration

In this study, Dianthus caryophyllus L. was used as the experimental plant to investigate the effects of rooting substrate and exogenous auxin concentration on the adventitious rooting of the stem cuttings. Our results showed that the formulated substrates with different physicochemical properties significantly affected the root formation. The substrate with a ratio of cocopeat to perlite at 1:1 (v:v) resulted in the optimum rooting of D. caryophyllus cuttings. Different Indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) concentrations affected the rooting percentage and seedling rate of D. caryophyllus. Application of NAA at 1000 mg·kg−1 with IBA at 100 mg·kg−1 resulted in the greatest rooting percentage and improved breeding speed. The rooting percentage and seedling rate did not increase with the increase in auxin concentration. Based on these results, we concluded that an appropriate rooting substrate is required to fulfill proper rooting of D. caryohhyllus cuttings, whereas an exogenous application of IBA and NAA at 1000 mg·kg−1 and 100 mg·kg−1 promoted the rooting and a higher auxin concertation inhibited rooting.

scholarly journals Propagation of Quercus robur L. (English Oak) by stem cuttings in Western Himalayas (Kashmir)

2014 ◽  
Vol 15 (1&2) ◽  
pp. 185-189
Author(s):  
Javaid Iqbal ◽  
V. Dutt ◽  
Hillal Ahmad ◽  
G.M. Bhat ◽  
P. A. Khan
Keyword(s):  
Acetic Acid ◽  
Quercus Robur ◽  
Naphthalene Acetic Acid ◽  
Western Himalayas ◽  
Stem Cuttings ◽  
Kashmir Valley ◽  
Indolebutyric Acid ◽  
Auxin Treatment ◽  
English Oak

Two experiments were conducted for propagation of Quercus robur L. by stem cuttings in Kashmir valley. First experiment which includes: hardwood cuttings were treated with Indolebutyric acid(IBA)having concentrations of 5,000, 10,000, 15,000, and 20,000 ppm in talc and Naphthalene acetic acid (NAA) having concentrations of 500, 1,000 and 1,500 ppm in talc while the second experiment i.e. softwood cuttings were taken and treated with Indolebutyric acid (IBA) having concentrations of 5,000, 10,000, 15,000, 20,000 ppm quick-dipped for 5-seconds and Naphthalene acetic acid (NAA) having concentrations of500, 1,000 and 1,500 ppm dipped for 24 hours and in both (nontreated) as control and placed under mist conditions. It was found that English Oak can be propagated through cuttings and auxin treatment is imperative and Indolebutyric acid (IBA) with concentration of 10,000 ppm showed best results with highest recorded rooting of 51.30 per cent in case of softwood cuttings and for hardwood cuttings also, Indolebutyric acid (IBA) with concentration of 10,000 ppm showed good results with highest recorded rooting of 29.70 per cent. However, control and NAA-treatments in both softwood and hardwood cuttings could not induce rooting at all.

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