scholarly journals Effects of Substrate and Exogenous Auxin on the Adventitious Rooting of Dianthus caryophyllus L.

HortScience ◽  
2020 ◽  
Vol 55 (2) ◽  
pp. 170-173
Author(s):  
Liang Zheng ◽  
Zibin Xiao ◽  
Weitang Song
Keyword(s):  
Acetic Acid ◽  
Physicochemical Properties ◽  
Root Formation ◽  
Dianthus Caryophyllus ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Experimental Plant ◽  
Stem Cuttings ◽  
Exogenous Auxin ◽  
Auxin Concentration

In this study, Dianthus caryophyllus L. was used as the experimental plant to investigate the effects of rooting substrate and exogenous auxin concentration on the adventitious rooting of the stem cuttings. Our results showed that the formulated substrates with different physicochemical properties significantly affected the root formation. The substrate with a ratio of cocopeat to perlite at 1:1 (v:v) resulted in the optimum rooting of D. caryophyllus cuttings. Different Indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) concentrations affected the rooting percentage and seedling rate of D. caryophyllus. Application of NAA at 1000 mg·kg−1 with IBA at 100 mg·kg−1 resulted in the greatest rooting percentage and improved breeding speed. The rooting percentage and seedling rate did not increase with the increase in auxin concentration. Based on these results, we concluded that an appropriate rooting substrate is required to fulfill proper rooting of D. caryohhyllus cuttings, whereas an exogenous application of IBA and NAA at 1000 mg·kg−1 and 100 mg·kg−1 promoted the rooting and a higher auxin concertation inhibited rooting.

scholarly journals Effect of naphthalene acetic acid on sprouting and rooting of stem cutting in Mussaenda species

2019 ◽  
Vol 30 (1) ◽  
pp. 48-54
Author(s):  
MHA Rashid ◽  
A Nahar
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Block Design ◽  
Stem Cutting ◽  
Naphthalene Acetic Acid ◽  
Combined Effects ◽  
Stem Cuttings ◽  
Treatment Combination ◽  
Red Flag ◽  
Number Of Leaves

Mussaendas are increasingly popular for the showy colour they provide during much of the year in garden landscapes. However, root formation in stem cuttings is a major challenge for cultivation of Mussaenda species. Therefore, the experiment was carried out at the Landscaping section of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh during the period from August to November 2018 to study the effect of phytohormone naphthalene acetic acid (NAA) on sprouting and rooting of stem cuttings in Mussaenda species. The two-factor experiment consisted of three Mussaenda species viz., Mussaenda frondosa (White flag bush), Mussaenda philippica (Pink flag bush) and Mussaenda erythrophylla (Red flag bush), and five concentrations of NAA viz., 0 (control), 0.1%, 0.2%, 0.3% and 0.4%. The experiment was laid out in randomised complete block design (RCBD) with three replications. The results revealed that all the traits related to sprouting and rooting potential of stem cuttings were significantly influenced by combined effects of Mussaenda species and various NAA concentrations. The maximum sprouted bud per cutting (3.57), highest number of roots per cutting (5.88) and the longest root length (4.32 cm) were recorded from M. philippica except number of leaves per stem cutting (4.42) in M. erythrophylla. The increasing concentrations of NAA application responded positively on sprouting and rooting characters in all three Mussaenda species used for this experiment. The best results obtained from the treatment where NAA was applied at the concentration of 0.3% with 3.92 sprouted buds per cutting, 4.77 leaves per cutting, 7.15 roots per cutting and 5.26 cm length of roots. The treatment combination of M. philippica (Pink flag bush) and 0.3% NAA, followed by NAA at 03% with M. erythrophylla (Red flag bush) was found to be best in terms of sprouting and rooting of stem cuttings in Mussaenda. Progressive Agriculture 30 (1): 48-54, 2019

scholarly journals Rooting Efficiency of Amur Maple Seedless Selections Produced by Mutagenesis1

2020 ◽  
Vol 38 (2) ◽  
pp. 37-43
Author(s):  
Andrzej K. Noyszewski ◽  
Alan G. Smith
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Customer Choice ◽  
Asexual Propagation ◽  
Propagation Methods ◽  
Noxious Weed ◽  
Acer Ginnala

Abstract Amur maple (Acer ginnala Maxim.) is a widely planted small tree with attractive red fall foliage. In many states, it is classified as a noxious weed and regulations restrict propagation and sale, limiting customer choice. Stem cuttings are commonly used as a means of asexual propagation of Amur maple, with softwood cuttings being the easiest to root. Here we report adventitious rooting efficiency of seedless Amur maple selections using four indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and four naphthalene acetic acid (NAA; 100, 500, 1,500, 5,000 ppm) treatments and a no hormone control. Overall, cuttings treated with 5,000 or 10,000 ppm IBA and 5,000 ppm NAA resulted in the highest percent rooting (PR), mean number of roots (MNR), and mean length of the longest root (MRL) across all seedless selections. Seedless selections SW-30-14 and SW-30-130 had the highest PR, 66% and 67%, respectively, and will perform well in commercial propagation. This level of rooting was greater than that of the cultivar ‘Bailey Compact' that had 46% PR, and the seedless selection SW-30-159 that had the lowest PR (10.3%), MNR (0.3), and MRL (0.2 in) averaged across all treatments. Cuttings from 4 of the 5 seedless selections treated with 1,500 ppm NAA had the greatest PR, whereas ‘Bailey Compact' and SW-30-159 had the greatest PR with 5,000 ppm NAA. Results indicate that rooting efficiency was impacted by mutagenesis in some selections, which may require optimization of propagation methods for those selections. Index words: adventitious rooting, auxin, vegetative propagation, sterile, non-native invasive. Species used in this study: Amur maple (Acer ginnala Maxim.). Chemicals used in this study: indole-3-butyric acid (IBA; 500, 1,500, 5,000, 10,000 ppm) and naphthalene acetic acid (NAA; 100 500, 1,500 5,000 ppm).

scholarly journals Cutting Type and Auxin Treatment Affect Rooting of Cupressus cashmeriana

2012 ◽  
Vol 30 (4) ◽  
pp. 211-213
Author(s):  
Annie Raup ◽  
Matthew D. Taylor
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Plant Material ◽  
Potassium Salt ◽  
Adventitious Rooting ◽  
Naphthalene Acetic Acid ◽  
Auxin Concentration ◽  
Evergreen Conifer ◽  
Significant Factors

Cupressus cashmeriana is an ornamental evergreen conifer with little published information on vegetative propagation. Two experiments were conducted to determine the effects of cutting type and auxin concentration and form on adventitious rooting. Experiment 1 evaluated three cutting treatments (softwood, hardwood, and mallet) and four auxin treatments consisting of 0, 2500, 5000, and 10000 ppm potassium salt of indole-3-butyric acid (K-IBA). Experiment 2 evaluated the same cutting types as experiment 1 and auxin treatments consisted of 5000 ppm K-IBA, 5000 ppm naphthalene acetic acid (NAA), 2500 ppm K-IBA in combination with 2500 ppm NAA and zero auxin applied. In both experiments, rooting of softwood and hardwood cuttings was significantly higher than mallet cuttings. Treatment of cuttings with different rates and types of auxin lead to inconclusive results, which indicate auxin may not be beneficial for rooting of C. cashmeriana. This research suggests that the age of plant material is one of the most significant factors for successful rooting C. cashmeriana.

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Adventitious Shoots ◽  
Conservation Strategy ◽  
Soil Conditions ◽  
Natural Soil ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Medicinal Tree ◽  
Couroupita Guianensis

The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of Couroupita guianensis (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds’ induction and their growth were observed with α-Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of C. guianensis were rooted and shoots were induced when pretreated with 400 mg L−1 NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg L−1 indole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg L−1 indole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of C. guianensis through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.

Multiple factors influence adventitious rooting in carnation (Dianthus caryophyllus L.) stem cuttings

2016 ◽  
Vol 81 (3) ◽  
pp. 511-521 ◽  
Author(s):  
Joan Villanova ◽  
Antonio Cano ◽  
Alfonso Albacete ◽  
Alfonso López ◽  
Emilio Á. Cano ◽  
...  
Keyword(s):  
Dianthus Caryophyllus ◽  
Adventitious Rooting ◽  
Stem Cuttings ◽  
Multiple Factors

Plant Regeneration from Cultured Medicago truncatula with Particular Reference to Abscisic Acid and Light Treatments

1998 ◽  
Vol 46 (1) ◽  
pp. 151 ◽  
Author(s):  
K. E. Nolan ◽  
R. J. Rose
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Plant Regeneration ◽  
Medicago Truncatula ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Marked Inhibition ◽  
Embryo Formation ◽  
Auxin Concentration

Medicago truncatula (Jemalong 2HA) can be regenerated by somatic embryogenesis utilising 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). There is a requirement for both NAA and BAP for callus induction and embryo formation. There is no requirement for a drop in auxin concentration to induce embryos. Abscisic acid (ABA) when present with NAA and BAP during embryo formation at a concentration of 1 µM, increases the number of embryos per callus. The ABA treatment stimulates embryo numbers in both light and darkness. The conversion efficiency of embryo to plant is unchanged irrespective of the presence of ABA during embryo formation, indicating that ABA does not improve the regeneration of the embryos once formed. Importantly, the presence of light in the embryo formation period causes a marked inhibition of embryo conversion.

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Adventitious rooting on the Brazilian red-cloak and sanchezia after application of indole-butyric and humic acids

2014 ◽  
Vol 32 (4) ◽  
pp. 434-439 ◽  
Author(s):  
Lílian EB Baldotto ◽  
Marihus A Baldotto
Keyword(s):  
Humic Acids ◽  
Dry Matter ◽  
Root Formation ◽  
Ornamental Plants ◽  
Adventitious Rooting ◽  
Dry Matter Accumulation ◽  
Regression Equations ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Number Of Leaves

Plant regulators, as well as bioactive fractions of the organic matter, can accelerate the development and increase the efficiency of adventitious rooting in ornamental plants. We evaluated rooting in the Brazilian red-cloak (Megaskepasma erythrochlamys) and sanchezia (Sanchezia nobilis) cuttings in response to application of indole-butyric acid (IBA) and humic acids (HA). Stem cuttings of both species treated with solutions of 0, 250, 500, 1000, 2000 mg/L of IBA and 0, 10, 20, 30, 40 mmol/L of C of HA were placed in 2.0 L plastic pots containing carbonized rice husk, kept in greenhouse. Survival of cuttings, number of leaves and shoot and root fresh and dry matter were evaluated 45 days after. Data were used to run an analysis of variance. We observed the average contrasts, calculated the regression equations and estimated the concentrations that provided the best efficiency in terms of root dry matter. For IBA, 185.4 and 66.8 mg/L were the concentrations that promoted the highest root dry matter accumulation in the Brazilian red-cloak and sanchezia, respectively, with increments of 140 and 22.5% in relation to the control. HA, at 33.6 mmol L-1 of C, increased shoot fresh and dry matter in the Brazilian red-cloak, but reduced root formation compared to the control. In sanchezia, HA at 19.5 mmol L-1 of C promoted increases of 35.9% in root dry matter in relation to the control. We concluded that IBA, in the abovementioned concentrations, increased adventitious rooting in cuttings of both the Brazilian red-cloak and sanchezia, while optimal doses of HA solutions are very likely to improve adventitious rooting in sanchezia, but not in the Brazilian red-cloak.

scholarly journals Mist Level Influences Vapor Pressure Deficit and Gas Exchange During Rooting of Juvenile Stem Cuttings of Loblolly Pine

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1448-1456 ◽  
Author(s):  
Anthony V. LeBude ◽  
Barry Goldfarb ◽  
Frank A. Blazich ◽  
John Frampton ◽  
Farrell C. Wise
Keyword(s):  
Vapor Pressure ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Vapor Pressure Deficit ◽  
Root Formation ◽  
Net Photosynthesis ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Ambient Conditions

Two experiments were conducted during which juvenile hardwood or softwood stem cuttings of loblolly pine (Pinus taeda L.) were rooted under six mist regimes in a polyethylene-covered greenhouse to investigate the effect of mist level on vapor pressure deficit (VPD) and cutting water potential (Ψcut), and to determine the relationships between these variables and rooting percentage. In addition, net photosynthesis at ambient conditions (Aambient) and stomatal conductance (gs) were measured in stem cuttings during adventitious root formation to determine their relationship to rooting percentage. Hardwood stem cuttings rooted ≥80% when mean daily VPD between 1000 and 1800 hr ranged from 0.60 to 0.85 kPa. Although rooting percentage was related to Ψcut, and Aambient was related to Ψcut, rooting percentage of softwood stem cuttings was not related to Aambient of stem cuttings. Using VPD as a control mechanism for mist application during adventitious rooting of stem cuttings of loblolly pine might increase rooting percentages across a variety of rooting environments.

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