scholarly journals VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

2019 ◽  
Vol 32 (4) ◽  
pp. 858-866
Author(s):  
GABRIELA TEODORO ROCHA ◽  
AYURE GOMES DA SILVA ◽  
JULIANA BEZERRA MARTINS ◽  
NEI PEIXOTO ◽  
FABRICIO RODRIGUES
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Spondias Tuberosa ◽  
Completely Randomized Design ◽  
Low Efficiency ◽  
Indole 3 Acetic Acid ◽  
Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

scholarly journals Induksi Kalus Kentang Asal Desa Dombu (Solanum tuberosum L.) Dengan Zpt Indole-3-Acetic Acid (IAA)

2019 ◽  
Vol 8 (2) ◽  
Author(s):  
Lyly Zulraufianti ◽  
Asri Pirade Paserang
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Solanum Tuberosum ◽  
Natural Science ◽  
Callus Induction ◽  
Solanum Tuberosum L ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Department Faculty ◽  
Indole 3 Acetic Acid

The research of callus induction in potato (S. tuberosum  L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed that  the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.

scholarly journals PENGARUH KOMBINASI IAA (Indole-3-Acetic Acid) DAN BAP (6-Benzylaminopurine) TERHADAP INISIASI TANAMAN VANILI (Vanilla planifolia Andrews)

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 157-166
Author(s):  
Apri Salfiani ◽  
Asri Pirade Paserang
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Fusarium Oxysporum ◽  
Maximum Concentration ◽  
Shoot Length ◽  
Vanilla Planifolia ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Indole 3 Acetic Acid

Provision of Vanilla (Vanilla planifolia Andrews) with traditional cultivation often has problems, including the availability of unhealthy seeds due to disease caused by Fusarium oxysporum. This disease can thwart vanilla plantations up to 85% because the pathogen can infect all parts of the vanilla plant making it difficult to control efforts. Alternative efforts were made to overcome this problem, namely through the initiation process in tissue culture by combining the hormones IAA (indole-3-acetic acid) and BAP (6-benzylaminopurine). This study aims to determine the effect and the maximum concentration of the combination of IAA and BAP on the initiation of vanilla plants. This study was conducted based on a completely randomized design (CRD) with 5 treatments and 3 repetitions. This treatment consisted of: A (MS + 0.1 ppm IAA + 1 ppm BAP), B (MS + 0.2 ppm IAA + 1 ppm BAP), C (MS + 0.3 ppm IAA + 1 ppm BAP), D (MS + 0.4 ppm IAA + 1 ppm BAP), and E (MS + 0.5 ppm IAA + 1 ppm BAP). Observations were made after 40 days from the planting process. The results showed that the concentration of the combination of IAA and BAP can affect the growth and organogenesis of the initiation of vanilla plants (Vanilla planifolia Andrews). Treatment C (MS + 0.3 ppm IAA + 1 ppm BAP) was the best concentration from this study, with average values: number of shoots (1), shoot length (1.73 cm), number of roots (1), and number of leaves (1.33).

scholarly journals Clonal propagation of Eucalyptus urophylla under effect of Cyperus rotundus extract and indole-3-acetic acid

2021 ◽  
Vol 17 (10) ◽  
Author(s):  
Matheus Gabriel Modesto Santos ◽  
Wesley Costa Silva ◽  
Pedro Henrique Pinto Ribeiro ◽  
Vitor Corrêa de Mattos Barretto ◽  
Ednaldo Cândido Rocha ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Length ◽  
Cyperus Rotundus ◽  
Root Induction ◽  
Eucalyptus Urophylla ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tuber Extract ◽  
Indole 3 Acetic Acid ◽  
Shed Light

Cyperus rotondus tubers possess substances with allelopathic qualities. Some of these substances can act as synergists of plant hormones and be used for root induction in cuttings. This study aimed to shed light on the development and rooting of Eucalyptus urophylla cuttings treated with C. rotondus (nutgrass) tuber extract and IAA (indole-3-acetic acid). The experiments were conducted using a completely randomized design (0; 25; 50; 75 and 100% nutgrass extract and 0; 50; 250; 500; 750 and 1000 mg L-1 of IAA), with four repetitions per treatment and four cuttings per plot. Plantlet height, stem width and root length were measured. The greatest width and height values were recorded for the control (0% nutgrass extract and 0 mg L-1 of IAA), indicating that these substances did not contribute to the development of these variables. However, treatments of nutgrass extract up to 50% were associated with greater root length. C. rotundus extract concentrations of 53% can be used to improve root development in E. urophylla cuttings, and contribute to the production of higher quality plantlets.

scholarly journals Effect of Indole Acetic Acid and Boron on the Nutrient Contents of Summer Tomato Cultivar Bina Tomato-3

2019 ◽  
pp. 1-7
Author(s):  
Munmun Saha ◽  
Md. Abul Khair Chowdhury ◽  
Md. Akhter Hossain Chowdhury
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Control Treatment ◽  
Tomato Cultivar ◽  
Tomato Fruits ◽  
Tomato Plants ◽  
Nutrient Contents ◽  
Randomized Design ◽  
Agricultural Chemistry ◽  
Completely Randomized Design

A pot experiment was carried out with tomato variety BINA Tomato-3 in the net house of the Department of Agricultural Chemistry, Bangladesh Agricultural University (BAU), Mymensingh from March to August, 2010 to study the effect of Indole Acetic Acid(IAA) and Boron(B) on the nutrient contents of BINA Tomato-3. There were four doses of IAA viz. 0, 20, 40 and 60 ppm and B viz. 0, 1.5, 2.0 and 2.5 kg ha-1. The experiment was laid out in a Completely Randomized Design (CRD) with 3 replications. The result revealed that nutrient contents of tomato fruits and plants such as N, P, Ca, Mg and B were significantly influenced by the application of IAA and B. The highest N (0.70%), Ca (0.69%), Mg (0.80%) in tomato fruits and highest N (0.40%), Ca (0.93%), Mg (0.90%) in tomato plants  were obtained in 60 ppm IAA along with 2.0 kg B ha-1 and the lowest was obtained in the control treatment. In tomato fruits the highest P (0.41%) was found in IAA40B2.0 treatment, while the lowest P (0.18%) was recorded in IAA0B0.0. The lowest B (4.55µgg-1) was recorded in control, while the highest B (15.72µgg-1) was found in IAA20B2.0in tomato fruits. In tomato plants the highest P (0.34%) was recorded in IAA60B1.5 and the lowest P (0.20%) was found in control. The lowest B (4.15µgg-1) was recorded in IAA0B0.0, while the highest B(13.33µgg-1) was found in IAA40B2.0 in tomato plants. The overall results suggest that treatment IAA60B2.0 was the best from other treatments.

scholarly journals Use of indole-3-acetic acid (IAA) in postharvest of cajá-manga (Spondias dulcis)

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
Franciele Cristian Da Silva ◽  
Hygor Amaral Santana ◽  
Juliana Oliveira Silva De Menezes ◽  
Mírian Carvalho Tavares ◽  
Rodolfo David Martins ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Climacteric Fruit ◽  
Randomized Design ◽  
Postharvest Life ◽  
Completely Randomized Design ◽  
Indole 3 Acetic Acid ◽  
Unripe Fruits

The cajá-manga (Spondias dulcis Parkinson) is an exotic fruit originally from the Polynesian Islands. It is a climacteric fruit, and the postharvest life of its fruits is very short, due to its fast ripening. Thus, this study aimed to evaluate the effect of auxin concentrations on delaying the ripening process of cajá-manga fruits. One hundred twenty cajá-manga fruits obtained from, Hidrolândia-GO, Brazil, at 16°55'32.35" S and 49°21'39.76" W). A completely randomized design in a 5×2+2 factorial scheme was used. Five indole-3-acetic acid (IAA) concentrations (0, 50, 100, 150 and 200 mg L-1), two moments of evaluations (at 5 and 10 days after IAA applications), and two additional treatments (ripe and unripe fruits) were evaluated. Evaluations were carried out every 5 days, in triplicates. The physicochemical variables, pulp firmness, titratable acidity (TA), soluble solids (SS), ascorbic acid content, and color of pulp and peel were evaluated. The concentrations of 50 and 100 mg L-1 of IAA presented the best results, causing a delay in ripening and the lowest sensory losses in the fruits of cajá-manga.

scholarly journals Vegetative Propagation of Gabirobeira Associate to Indolbutyric Acid in Different Seasons

2019 ◽  
Vol 11 (7) ◽  
pp. 187
Author(s):  
Lasara K. F. Souza ◽  
Lana L. L. Dias ◽  
Moab A. Barbosa ◽  
Diego I. Rocha ◽  
Edésio F. Reis ◽  
...  
Keyword(s):  
Vegetative Propagation ◽  
Stem Cuttings ◽  
Spring Season ◽  
Rooted Cuttings ◽  
Randomized Design ◽  
Different Seasons ◽  
Completely Randomized Design ◽  
Adventitious Rhizogenesis

The propagation of gabirobeira is carried out by seeds. However, the recalcitrance and not tolerance of the seeds to the storage compromise the formation of seedlings. Thus, the propagation by cuttings can be an option, being able to provide significant results in different seasons. The objective of this work was to evaluate the vegetative propagation by stem cuttings of two species of gabirobeira (Campomanesia adamantium and Campomanesia pubescens) associate to indolbutyric acid (IBA) at differents seasons of the year. Cuttings of the two species were obtained in the 4 seasons and concentrations of 0, 2000, 4000, 6000 and 8000 mg L-1 of IBA were used, by immersion in the solution for 15 seconds and sequentially staked on trays containing a sand substrate. Sixty days after experiments were set it was evaluated the percentage of cuttings with shoots, live cuttings, dead cuttings, cuttings with calluses and rooted cuttings. A completely randomized design with 5 treatments, 4 replicates and 8 cuttings per plot was adopted. During the whole experiment, the greenhouse, substrate and cutting leaves temperature was monitored. The use of IBA did not promote adventitious rhizogenesis, but provided a high percentage of survival for the species Campomanesia adamantium and Campomanesia pubescens by stem cuttings during the spring season.

THE SOURCES OF AUXIN HORMONE TO GROWTH OF SHOOT PINEAPPLE STEM CUTTING (Ananascomosus.L. Merr.)

2016 ◽  
Vol 1 (1) ◽  
pp. 45-52
Author(s):  
Palupi Puspitorini
Keyword(s):  
Water Concentration ◽  
Urine Concentration ◽  
Experimental Results ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Natural Hormone ◽  
Cow Urine ◽  
Urine Concentrations ◽  
Completely Randomized Design ◽  
Better Than

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.           

scholarly journals RESGATE E PROPAGAÇÃO VEGETATIVA DE Sequoia sempervirens

FLORESTA ◽  
2014 ◽  
Vol 45 (2) ◽  
pp. 383 ◽  
Author(s):  
Márcio Carlos Navroski ◽  
Mariane De Oliveira Pereira ◽  
André Felipe Hess ◽  
Raul Silvestre ◽  
Alessandro Camargo Ângelo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Good Option ◽  
Sequoia Sempervirens ◽  
Southern Brazil ◽  
Clonal Forestry ◽  
Rooted Cuttings ◽  
Clonal Multiplication ◽  
Conventional Breeding

Objetivou-se com o estudo verificar resgate vegetativo, enraizamento de estacas e adaptação das mudas enraizadas em sistema de minijardim clonal de Sequoia sempervirens. Realizou-se a decepa de cinco indivíduos com 20 anos de idade e após foi contabilizada a capacidade de emissão de brotações. Com as brotações, foram confeccionadas estacas, as quais foram submetidas a diferentes concentrações de ácido indolacético (AIA) (0, 2000, 4000 e 6000 mg.L-1). Foram avaliadas a porcentagem de enraizamento e o número e comprimento de raízes 160 dias após o estaqueamento. O experimento foi instalado em DIC, com 5 repetições de 10 estacas. Após a formação das mudas com as estacas enraizadas, elas foram transplantadas para vasos, para a formação do minijardim clonal. O resgate vegetativo através da decepa de árvores apresentou potencialidade para produção de estacas de sequoia e revigoramento do material. O uso entre 2000 e 4000 mg.L-1 de AIA aumentou a porcentagem de enraizamento e também o número e comprimento de raízes formadas. O minijardim clonal de sequoia mostrou ser uma boa opção para a multiplicação clonal da espécie. A clonagem da sequoia pode auxiliar tanto no melhoramento convencional como em plantios comerciais da espécie, principalmente no Sul do Brasil, local onde encontra condições adequadas para seu crescimento.Palavras-chave: Silvicultura clonal; coníferas; rejuvenescimento; minijardim clonal. AbstractRescue and vegetative propagation of Sequoia sempervirens. The objective of the study to verify the vegetative rescue, rooting cuttings and rooted plants adapt in mini-clonal hedge of Sequoia sempervirens system. Was held coppicing five individuals aged 20 years old and was recorded after the ability to shoot emission. Shoots with different concentrations of indole acetic acid were applied (IAA) (0, 2000, 4000 and 6000 mg.L-1) in cuttings made by evaluating the percentage of rooting, number and length of roots 160 days after striking. The experiment was a completely randomized with five replicates of 10 cuttings. After the formation of seedlings with rooted cuttings, these were transplanted to pots for the formation of mini-clonal hedge. The vegetative rescue through coppicing trees showed potential for the production of cuttings sequoia and reinvigoration of the material. The use from 2000 to 4000 mg.L-1 IAA increased rooting percentage and the number and length of roots formed. The mini-clonal hedge of sequoia proved to be a good option for clonal multiplication of the species. Cloning of sequoia can help both conventional breeding and for commercial plantations of the species, especially in southern Brazil, where finds suitable conditions for their growth.Keywords: Clonal forestry; conifers; rejuvenation; mini-clonal hedge.

scholarly journals Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Adventitious Shoots ◽  
Conservation Strategy ◽  
Soil Conditions ◽  
Natural Soil ◽  
Naphthalene Acetic Acid ◽  
Stem Cuttings ◽  
Medicinal Tree ◽  
Couroupita Guianensis

The present study explores the potential of exogenous auxins in the development of adventitious shoots and roots from shoot cuttings of Couroupita guianensis (Nagalingam), a threatened tree. Experiments were conducted to assess the effect of various concentrations of auxins on shoot and root morphological traits of stem cuttings in the greenhouse. Amongst the auxins tested, significant effects on number of shoot buds’ induction and their growth were observed with α-Naphthalene Acetic Acid (NAA) treated nodal cuttings. Cent percentage of the stem cuttings of C. guianensis were rooted and shoots were induced when pretreated with 400 mg L−1 NAA for 5 min. Maximum 79% of stem cuttings responded to pretreatment of 300 mg L−1 indole-3-butyric acid (IBA) for 5 min, and 75% of stem cuttings induced shoots with 400 mg L−1 indole-3-acetic acid (IAA). Presence of at least 5 nodes on stem cuttings was found to be prerequisite for root and shoot induction. About 92% of plants were survived under natural soil conditions raised from the stem cuttings. This is the first report of vegetative propagation of C. guianensis through stem cuttings which could be used for conservation strategy and sustainable utilization of this threatened medicinal tree.

scholarly journals Screening of Indole-3-Acetic Acid (IAA) Productions by Endophytic Fusarium oxysporum Isolated from Phyllanthus niruri

1970 ◽  
Vol 7 (1) ◽  
pp. 56-59
Author(s):  
Abdul Raffi Junaidi ◽  
Mohamad Hasnul Bolhassan
Keyword(s):  
Acetic Acid ◽  
Fusarium Oxysporum ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Indole Acetic Acid ◽  
Phyllanthus Niruri ◽  
Acetic Acid Production ◽  
Indole Acetic Acid Production ◽  
Indole 3 Acetic Acid

Ten newly isolated endophytic fungi from Phyllathus niruri Linn. were identified as Fusarium oxysporum. These isolates were screened out for their productivity of indole acetic acid (IAA) by the salkowski's method. Out of these isolates, two isolates showed high amount of indole acetic acid production, which were FO9 and FO10 with concentration of 23.52 μg/ml and 5.95 μg/ml, respectively.

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