scholarly journals Biodistribution and Tumors MRI Contrast Enhancement of Magnetic Nanocubes, Nanoclusters, and Nanorods in Multiple Mice Models

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
V. Naumenko ◽  
A. Garanina ◽  
A. Nikitin ◽  
S. Vodopyanov ◽  
N. Vorobyeva ◽  
...  
Keyword(s):  
Contrast Enhancement ◽  
Tumor Imaging ◽  
Atomic Emission ◽  
Mri Contrast Agents ◽  
Mri Contrast ◽  
Magnetic Nanorods ◽  
Tumor Diagnostics ◽  
Magnetic Resonance Imaging Mri ◽  
4T1 Breast Cancer

Magnetic resonance imaging (MRI) is a powerful technique for tumor diagnostics. Iron oxide nanoparticles (IONPs) are safe and biocompatible tools that can be used for further enhancing MR tumor contrasting. Although numerous IONPs have been proposed as MRI contrast agents, low delivery rates to tumor site limit its application. IONPs accumulation in malignancies depends on both IONPs characteristics and tumor properties. In the current paper, three differently shaped Pluronic F-127-modified IONPs (nanocubes, nanoclusters, and nanorods) were compared side by side in three murine tumor models (4T1 breast cancer, B16 melanoma, and CT26 colon cancer). Orthotopic B16 tumors demonstrated more efficient IONPs uptake than heterotopic implants. Magnetic nanocubes (MNCb) had the highest r2-relaxivity in vitro (300 mM−1·s−1) compared with magnetic nanoclusters (MNCl, 104 mM−1·s−1) and magnetic nanorods (MNRd, 51 mM−1·s−1). As measured by atomic emission spectroscopy, MNCb also demonstrated better delivery efficiency to tumors (3.79% ID) than MNCl (2.94% ID) and MNRd (1.21% ID). Nevertheless, MNCl overperformed its counterparts in tumor imaging, providing contrast enhancement in 96% of studied malignancies, whereas MNCb and MNRd were detected by MRI in 73% and 63% of tumors, respectively. Maximum MR contrasting efficiency for MNCb and MNCl was around 6-24 hours after systemic administration, whereas for MNRd maximum contrast enhancement was found within first 30 minutes upon treatment. Presumably, MNRd poor MRI performance was due to low r2-relaxivity and rapid clearance by lungs (17.3% ID) immediately after injection. MNCb and MNCl were mainly captured by the liver and spleen without significant accumulation in the lungs, kidneys, and heart. High biocompatibility and profound accumulation in tumor tissues make MNCb and MNCl the promising platforms for MRI-based tumor diagnostics and drug delivery.

scholarly journals Genetic encoding of targeted MRI contrast agents for in vivo tumor imaging

2019 ◽  
Author(s):  
Simone Schuerle ◽  
Maiko Furubayashi ◽  
Ava P. Soleimany ◽  
Tinotenda Gwisai ◽  
Wei Huang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Contrast Agents ◽  
Tumor Imaging ◽  
Mri Contrast Agents ◽  
Contrast Effects ◽  
Mri Contrast ◽  
Magnetic Resonance Imaging Mri ◽  
Targeted Mri

AbstractTumor-selective contrast agents have the potential to aid in the diagnosis and treatment of cancer using noninvasive imaging modalities such as magnetic resonance imaging (MRI). Such contrast agents can consist of magnetic nanoparticles incorporating functionalities that respond to cues specific to tumor environments. Genetically engineering magnetotactic bacteria to display peptides has been investigated as a means to produce contrast agents that combine the robust image contrast effects of magnetosomes with transgenic targeting peptides displayed on their surface. This work reports the first use of magnetic nanoparticles that display genetically-encoded pH low insertion peptide (pHLIP), a long peptide intended to enhance MRI contrast by targeting the extracellular acidity associated with the tumors. To demonstrate the modularity of this versatile platform to incorporate diverse targeting ligands by genetic engineering, we also incorporated the cyclic αv integrin-binding peptide iRGD into separate magnetosomes. Specifically, we investigate their potential for enhanced binding and tumor imaging both in vitro and in vivo. Our experiments indicate that these tailored magnetosomes retain their magnetic properties, making them well-suited as T2 contrast agents, while exhibiting increased binding compared to wild-type magnetosomes.

scholarly journals Magneto-Liposomes as MRI Contrast Agents: A Systematic Study of Different Liposomal Formulations

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 889 ◽  
Author(s):  
Nina Kostevšek ◽  
Calvin C. L. Cheung ◽  
Igor Serša ◽  
Mateja Erdani Kreft ◽  
Ilaria Monaco ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Lipid Bilayer ◽  
Mri Contrast Agents ◽  
Mri Contrast ◽  
Liposomal Formulations ◽  
Icp Ms ◽  
Magnetic Resonance Imaging Mri ◽  
Cancerous Cells ◽  
Selection Of

The majority of the clinically approved iron oxide nanoparticles (IO NPs) used as contrast agents for magnetic resonance imaging (MRI) have been withdrawn from the market either due to safety concerns or lack of profits. To address this challenge, liposomes have been used to prepare IO-based T2 contrast agents. We studied the influence of different phospholipids on the relaxivity (r2) values of magneto-liposomes (MLs) containing magnetic NPs in the bilayer, where a strong correlation between the bilayer fluidity and r2 is clearly shown. Embedding 5-nm IO NPs in the lipid bilayer leads to a significant improvement in their relaxivity, where r2 values range from 153 ± 5 s−1 mM−1 for DPPC/cholesterol/DSPE-PEG (96/50/4) up to 673 ± 12 s−1 mM−1 for DOPC/DSPE-PEG (96/4), compared to “free” IO NPs with an r2 value of 16 s−1 mM−1, measured at 9.4 T MRI scanner. In vitro MRI measurements, together with the ICP-MS analysis, revealed MLs as highly selective contrast agents that were preferentially taken up by cancerous T24 cells, which led to an improvement in the contrast and an easier distinction between the healthy and the cancerous cells. A careful selection of the lipid bilayer to prepare MLs could offer efficient MRI contrast agents, even at very low IO NP concentrations.

scholarly journals In Vivo Potential of Manganese Chelated Porphysomes as MRI Contrast Agents

2017 ◽  
Vol 3 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Chris J. Zhang ◽  
Michael S. Valic ◽  
Juan Chen ◽  
Gang Zheng
Keyword(s):  
Mri Contrast Agents ◽  
Resonance Imaging ◽  
Mri Contrast ◽  
Metal Chelating ◽  
Particle Stability ◽  
Quenching Efficiency ◽  
Magnetic Resonance Imaging Mri ◽  
Porphyrin Moiety

Porphysome nanoparticles are composed of porphyrin-conjugated lipids. The attachment of the porphyrin moiety to each phospholipid confers novel properties to the liposome-like nanoparticle, allowing it to perform a variety of diagnostic and therapeutic applications. The metal chelating properties of porphyrin can be used to bind manganese (Mn), transforming the porphysome into a contrast agent for magnetic resonance imaging (MRI). Previous work has extensively characterized the properties of the Mn-porphysome. Herein, we build upon that work by demonstrating the bio-interactions of Mn-porphysomes in vitro to validate their study in vivo. Particle stability in serum was inferred from fluorescence quenching efficiency, and tolerability to cells was measured using an MTT assay. Mn-porphysomes remained >80% quenched after 14H and showed no toxicity to cells at concentrations below 125 mM. These preliminary results suggest that the porphysome may be used to enhance MRI contrast in vivo.

scholarly journals Peptide-Based Soft Hydrogels Modified with Gadolinium Complexes as MRI Contrast Agents

2020 ◽  
Vol 13 (2) ◽  
pp. 19 ◽  
Author(s):  
Enrico Gallo ◽  
Carlo Diaferia ◽  
Enza Di Gregorio ◽  
Giancarlo Morelli ◽  
Eliana Gianolio ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Self Assembly ◽  
In Vitro Cytotoxicity ◽  
Mri Contrast Agents ◽  
Mri Contrast ◽  
High Relaxivity ◽  
Diagnostic Agents ◽  
Magnetic Resonance Imaging Mri ◽  
Branched Nanostructures

Poly-aromatic peptide sequences are able to self-assemble into a variety of supramolecular aggregates such as fibers, hydrogels, and tree-like multi-branched nanostructures. Due to their biocompatible nature, these peptide nanostructures have been proposed for several applications in biology and nanomedicine (tissue engineering, drug delivery, bioimaging, and fabrication of biosensors). Here we report the synthesis, the structural characterization and the relaxometric behavior of two novel supramolecular diagnostic agents for magnetic resonance imaging (MRI) technique. These diagnostic agents are obtained for self-assembly of DTPA(Gd)-PEG8-(FY)3 or DOTA(Gd)-PEG8-(FY)3 peptide conjugates, in which the Gd-complexes are linked at the N-terminus of the PEG8-(FY)3 polymer peptide. This latter was previously found able to form self-supporting and stable soft hydrogels at a concentration of 1.0% wt. Analogously, also DTPA(Gd)-PEG8-(FY)3 and DOTA(Gd)-PEG8-(FY)3 exhibit the trend to gelificate at the same range of concentration. Moreover, the structural characterization points out that peptide (FY)3 moiety keeps its capability to arrange into β-sheet structures with an antiparallel orientation of the β-strands. The high relaxivity value of these nanostructures (~12 mM−1·s−1 at 20 MHz) and the very low in vitro cytotoxicity suggest their potential application as supramolecular diagnostic agents for MRI.

Biocompatible dextran-coated gadolinium-doped cerium oxide nanoparticles as MRI contrast agents with high T1 relaxivity and selective cytotoxicity to cancer cells

2021 ◽  
Author(s):  
Anton Popov ◽  
Maxim Artemovich Abakumov ◽  
Irina Savintseva ◽  
Artem Ermakov ◽  
Nelly Popova ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Mri Contrast Agents ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Resonance Imaging ◽  
Mri Contrast ◽  
Selective Cytotoxicity ◽  
Magnetic Resonance Imaging Mri

Gd-based complexes are widely used as magnetic resonance imaging (MRI) contrast agents. The safety of previously approved contrast agents is questionable and is being re-assessed. The main causes of concern...

scholarly journals Study on Establishing Reference Safe Concentrations of MRI Contrast Agents for Optimized Images: Paramagnetic Gd-DTPA-BMEA and Superparamagnetic Ferucarbotran

2021 ◽  
Vol 11 (3) ◽  
pp. 1165
Author(s):  
Wen-Tien Hsiao ◽  
Yi-Hong Chou ◽  
Jhong-Wei Tu ◽  
Ai-Yih Wang ◽  
Lu-Han Lai
Keyword(s):  
Contrast Agent ◽  
Contrast Agents ◽  
Mri Contrast Agents ◽  
Mri Contrast Agent ◽  
In Vitro Experiments ◽  
Mri Contrast ◽  
In Vivo Experiments ◽  
Contrast Agent Injection

The purpose of this study is to establish the minimal injection doses of magnetic resonance imaging (MRI) contrast agents that can achieve optimized images while improving the safety of injectable MRI drugs. Gadolinium-diethylenetriamine penta-acetic acid (Gd-DTPA) and ferucarbotran, commonly used in clinical practice, were selected and evaluated with in vitro and in vivo experiments. MRI was acquired using T1-weighted (T1W) and T2-weighted (T2W) sequences, and the results were quantitatively analyzed. For in vitro experiments, results showed that T1W and T2W images were optimal when Gd-DTPA-bisamide (2-oxoethyl) (Gd-DTPA-BMEA) and ferucarbotran were diluted to a volume percentage of 0.6% and 0.05%; all comparisons were significant differences in grayscale statistics using one-way analysis of variance (ANOVA). For in vivo experiments, the contrast agent with optimal concentration percentages determined from in vitro experiments were injected into mice with an injection volume of 100 μL, and the images of brain, heart, liver, and mesentery before and after injection were compared. The statistical results showed that the p values of both T1W and T2W were less than 0.001, which were statistically significant. Under safety considerations for MRI contrast agent injection, optimized MRI images could still be obtained after reducing the injection concentration, which can provide a reference for the safety concentrations of MRI contrast agent injection in the future.

Water-soluble magnetic CoO nanocrystals functionalized with surfactants as T2-weighed MRI contrast agents in vitro

2011 ◽  
Vol 40 (14) ◽  
pp. 3616 ◽  
Author(s):  
Hong Yang ◽  
Hong Zhou ◽  
Cuixia Zhang ◽  
Xuejian Li ◽  
He Hu ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Mri Contrast Agents ◽  
Water Soluble ◽  
Mri Contrast

scholarly journals Preparation and application of various nanoparticles in biology and medicine

2013 ◽  
Vol 3 (1) ◽  
pp. 2 ◽  
Author(s):  
Vardan Gasparyan
Keyword(s):  
Magnetic Resonance Imaging ◽  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Optical Properties ◽  
Mri Contrast Agents ◽  
Resonance Imaging ◽  
Gold And Silver Nanoparticles ◽  
Mri Contrast ◽  
Cellular Processes ◽  
Magnetic Resonance Imaging Mri

The present paper considers prospects for application of various nanoparticles in biology and medicine. Here are presented data on preparation of gold and silver nanoparticles, and effects of shape of these nanoparticles on their optical properties. Application of these nanoparticles in diagnostics, for drug delivery and therapy, and preparation of magnetic nanoparticles from iron and cobalt salts are also discussed. Application of these nanoparticles as magnetic resonance imaging (MRI) contrast agents and as vehicles for drug delivery, and preparation of quantum dots and their application as prospective nanoparticles for multiplex analysis and for visualization of cellular processes will be tackled. Finally, prospects for new types of nanocomposites (metallic nano-shells) will be not overlooked.

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