Effect of Chitosan Nanoparticle-Loaded Thymus serpyllum on Hydrogen Peroxide-Induced Bone Marrow Stromal Cell Damage

We have determined the protective effects of Thymus serpyllum (TS) extract and nanoparticle-loaded TS on hydrogen peroxide-induced cell death of mesenchymal stromal cells (MSCs) in vitro. Gas chromatography–mass spectroscopy confirmed the spectrum of active components in the extract. Out of the three different extracts, the hexane extract showed significant free radical scavenging activity. Treatment of MSCs with H2O2 (hydrogen peroxide) significantly increased intracellular cell death; however, pretreatment with TS extract and nanoparticle-loaded TS (200 μg/ml) suppressed H2O2-induced elevation of Cyt-c and MMP13 and increased the survival rates of MSCs. H2O2-induced (0.1 mM) changes in cytokines were attenuated in the extract and nanoparticles by pretreatment and cotreatment at two time points (p<0.05). H2O2 increased cell apoptosis. In contrast, treatment with nanoparticle-loaded TS suppressed the percentage of apoptosis considerably (p<0.05). Therefore, TS may be considered as a potential candidate for enhancing the effectiveness of MSC transplantation in cell therapy.

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Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

International Journal of Phytomedicine ◽

10.5138/09750185.2123 ◽

2017 ◽

Vol 9 (4) ◽

pp. 615

Author(s):

Mukesh Kumar Yadav ◽

Santosh Kumar Singh ◽

JS Tripathi ◽

YB Tripathi

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Centella Asiatica ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Phytochemical Screening

Centella asiatica also known as mandukparni or Indian pennywort or jalbrahmi, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. Centella asiatica has long been used to improve memory and cognitive function.The study aimed to identify the phytochemicals present in different solvent extracts of Centella asiatica (i.e. PECA- Petroleum ether extract of C. asiatica, CCA- Chloroform extract of C. asiatica, EACA- Ethyl acetate extract of C. asiatica, ECA- Ethanolic extract of C. asiatica, HACA- Hydro-alcoholic extract of C. asiatica) and evaluate the respective in-vitro antioxidant potentials. The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of Centella asiatica was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.In all different solvent extracts of C. asiatica revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with EC50 values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC50 values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity. The present study indicates that the Centella asiatica extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.

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Antioxidant Activity of Metabolites from Coleonema Album (Rutaceae)

Natural Product Communications ◽

10.1177/1934578x0600100505 ◽

2006 ◽

Vol 1 (5) ◽

pp. 1934578X0600100 ◽

Cited By ~ 2

Author(s):

Lindy L. Esterhuizen ◽

Riaan Meyer ◽

Ian A. Dubery

Keyword(s):

Antioxidant Activity ◽

South African ◽

Structural Information ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Active Components ◽

Bioassay Guided Fractionation ◽

The Relationship

Coleonema album, a member of the South African ‘Fynbos’ biome, was evaluated for its antioxidant and free radical scavenging activity. Ethanol- and acetone-based extracts from plant material obtained from two different geographical areas were analysed. A bioassay-guided fractionation methodology was followed for screening of active compounds. The 1,1-diphenyl-2-picrylhydrazyl (DPPH)-TLC method revealed the presence of a number of antioxidants which were quantified by the DPPH-spectrophotometric assay and the oxygen radical absorbance capacity (ORAC) assay. The C. album extracts possessed significant in vitro antioxidant activity, a large portion of which appeared to be contributed by the phenolic compounds. In contrast, the reducing power of the extracts could not be correlated with the observed antioxidant activity. Identification and structural information of the active components were obtained by a combination of preparative TLC and LC-MS which revealed the presence of coumarin aglycones and glycosides. The results of this study indicate that C. album contains strong antioxidants that warrant further investigation into the relationship between the structure and activity of the active coumarin metabolites.

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Lanostane Triterpenoid Metabolites from a Penares sp. Marine Sponge Protect Neuro-2a Cells against Paraquat Neurotoxicity

Molecules ◽

10.3390/molecules25225397 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5397

Author(s):

Ekaterina A. Yurchenko ◽

Sophia A. Kolesnikova ◽

Ekaterina G. Lyakhova ◽

Ekaterina S. Menchinskaya ◽

Evgeny A. Pislyagin ◽

...

Keyword(s):

Marine Sponge ◽

Cell Damage ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Neuroprotective Activity ◽

Hsp70 Expression ◽

Protective Effects ◽

Intracellular Level ◽

Intracellular Ros ◽

Lanostane Triterpenoid

The results of an investigation of the protective effects of five lanostane triterpenoids: 3β-acetoxy-7β,8β-epoxy-5α-lanost-24-en-30,9α-olide (1), 3β-hydroxy-7β,8β-epoxy-5α-lanost-24-en- 30,9α-olide (2), 29-nor-penasterone (3), penasterone (4), and acetylpenasterol (5), from a marine sponge, Penares sp., against paraquat-induced neuroblastoma Neuro-2a cell damage, are described. The influence of all compounds on viability of the Neuro-2a cells treated with paraquat (PQ) was studied with MTT and fluorescein diacetate assays as well as propidium iodide straining. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the compounds as well as their influence on reactive oxygen species (ROS) level and mitochondrial membrane potential in PQ-treated neuronal cells were analyzed. Finally, the effect of the compounds on intracellular level of heat shock protein 70 kDa (Hsp70) and neurite outgrowth in PQ-treated Neuro-2a cells were studied. Studied triterpenoids demonstrated protective effects against PQ-induced neurotoxicity associated with the ability to reduce ROS intracellular level and diminish mitochondrial dysfunction. Acetylpenasterol (5), as a more promising neuroprotective compound, significantly increased the viability of Neuro-2a cells incubated with PQ as well as decreased intracellular ROS level in these cells. Moreover, acetylpenasterol induced Hsp70 expression in PQ-treated cells. It was also shown to inhibit PQ-induced neurite loss and recovered the number of neurite-bearing cells. The relationship between neuroprotective activity of the investigated compounds 1–5 and their chemical structure was also discussed.

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In Vitro Antioxidant Activities of Polysaccharides from Auricularia auriada

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.104 ◽

2011 ◽

Vol 183-185 ◽

pp. 104-109

Author(s):

Ning Zhang ◽

Zhen Yu Wang ◽

Hua Zhang ◽

Jian Ren ◽

Xiu Mei Hao

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Water Soluble ◽

Peroxide Radical ◽

Scavenging Effect ◽

Radical Scavenging Effect ◽

Chelating Ability

This study aims to evaluate the antioxidative activities of water and alkaline solution pH=8, 50% ethanolic (APP1 or SAPP1), 75% ethanolic (APP2 or SAPP2) and 100% ethanolic (APP3 or SAPP3) extracts of A. auricular. The antioxidant activities, including the ABTS, superoxide anion radical-scavenging effect, hydrogen peroxide radical-scavenging effect and chelating ability on ferrous ions were studied in vitro. A comparison of the 50% effective concentration (EC50) values of different antioxidative reactions revealed that SAPP3 was more effective in scavenging ABTS, hydrogen peroxide and chelating ability on ferrous ions.SAPP2 showed a higher superoxide radical-scavenging activity. The alkali-soluble polysaccharides extracts showed higher radical-scavenging effect than water-soluble polysaccharides.

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Protective Effects of Berry Extracts on Hydrogen Peroxide-induced Rat Brain Neuronal Cell Damage in vitro

Journal of Food and Nutrition Research ◽

10.12691/jfnr-2-6-2 ◽

2014 ◽

Vol 2 (6) ◽

pp. 277-280 ◽

Cited By ~ 3

Author(s):

Chang-Ho Jeong ◽

Chi-Woen Jang ◽

Dong-Chan Kum ◽

Koo Yul Lee ◽

Seung Yuan Lee ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Rat Brain ◽

Cell Damage ◽

Neuronal Cell ◽

Protective Effects ◽

Berry Extracts

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Antidepressant Effects of Vaccinium bracteatum via Protection Against Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis

The American Journal of Chinese Medicine ◽

10.1142/s0192415x18500775 ◽

2018 ◽

Vol 46 (07) ◽

pp. 1499-1518 ◽

Cited By ~ 3

Author(s):

Dool-Ri Oh ◽

Yujin Kim ◽

Eun-Jin Choi ◽

Ara Jo ◽

Jawon Shin ◽

...

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Cell Death ◽

Neuronal Damage ◽

In Vitro Models ◽

Monoamine Oxidase A ◽

Protective Effects ◽

Mao A ◽

Anti Oxidant

The present study evaluates the anti-oxidative stress activity of Vaccinium bracteatum Thunb. fruit extract (VBFW) to identify the mechanisms responsible for its antidepressant-like effects. To evaluate the antidepressant and anti-oxidant effects of VBFW, malondialdehyde (MDA), serotonin transporter (SERT), and monoamine oxidase A (MAO-A) levels were measured in a mouse model of chronic restraint stress (CRS). The underlying mechanisms preventing oxidative stress and neuronal apoptosis were investigated using in vitro models of hydrogen peroxide (H2O[Formula: see text]-induced neuronal damage. The results showed that VBFW treatment (200[Formula: see text]mg/kg) significantly reduced MDA, SERT, and MAO-A levels in the prefrontal cortex of CRS mice. Furthermore, VBFW (30[Formula: see text][Formula: see text]g/mL) exhibited protective effects against H2O2-induced cell death via inhibition of the H2O2-induced increase in Bax and decrease in Bcl-2 levels within the mitochondria of SH-SY5Y cells. Furthermore, VBFW (10 and 30[Formula: see text][Formula: see text]g/mL) exerted protective effects against H2O2-induced cell death through inhibition of key mitochondria-associated apoptotic proteins such as cytochrome c, caspase-3 and PARP. Additionally, VBFW (10 and 30[Formula: see text][Formula: see text]g/mL) could improve the activity of anti-oxidant enzymes (such as SOD and catalase) in H2O2-treated SH-SY5Y cells. These results suggest that the antidepressant and anti-oxidant effects of VBFW might be mediated by the regulation of SERT and MAO-A, and possibly associated with regulation of oxidative stress-induced apoptosis.

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Design, Synthesis, and Biological Evaluation of Novel Tetramethylpyrazine- nitrone Derivatives as antioxidants

Letters in Drug Design & Discovery ◽

10.2174/1570180817999201117145311 ◽

2020 ◽

Vol 17 ◽

Author(s):

Haiyun Chen ◽

Chunyan Yan ◽

Jie Cao ◽

Zheng Liu ◽

Yewei Sun ◽

...

Keyword(s):

Ischemic Stroke ◽

Neurite Outgrowth ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Iodoacetic Acid ◽

Biological Evaluation ◽

Protective Effects ◽

Neuroprotective Effects ◽

Design Synthesis

Background: Thrombolysis and endovascular thrombectomy acted as two mainly therapeutic strategies for the ischemic stroke in clinic. However, reperfusion injury caused oxidative stress leading overproduction of reactive oxygen species, mitochondrial dysfunction and subsequent cell death. Methods: We designed and synthesized two tetramethylpyrazine-nitrone derivatives (T-003 and T-005) and investigated their abilities for scavenging free radicals and protective effects as well as neurite outgrowth promotion in vitro. Results and Discussion: Both of them showed potent radical-scavenging activity and neuroprotective effects against iodoacetic acid-induced cells injury. Furthermore, T-003 and T-005 significantly promoted neurite outgrowth in PC12 cells. Conclusion: Our results suggest that compound T-003 and T-005 could be potent antioxidants for the treatment of neurological disease, particularly ischemic stroke.

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Protective Effects of Oenothera biennis against Hydrogen Peroxide-Induced Oxidative Stress and Cell Death in Skin Keratinocytes

Life ◽

10.3390/life10110255 ◽

2020 ◽

Vol 10 (11) ◽

pp. 255

Author(s):

Seung Young Lee ◽

Chul Hwan Kim ◽

Buyng Su Hwang ◽

Kyung-Min Choi ◽

In-Jun Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Hacat Cells ◽

Scavenging Activity ◽

Protective Effects ◽

Oenothera Biennis ◽

Skin Keratinocytes

Background: Oenothera biennis (evening primrose) produces bioactive substances with a diverse range of pharmacological functions. However, it is currently unknown whether extract prepared from the aerial parts of O. biennis (APOB) can protect the skin against oxidative stress. Objective: The aim of this study is to investigate the protective effects of APOB against oxidative stress-induced damage in human skin keratinocytes (HaCaT) and elucidate the underlying mechanisms. Methods: We pretreated HaCaT cells with various concentrations of APOB or the antioxidant N-acetyl-L-cysteine before applying H2O2. We then compared the cell viability, intracellular reactive oxygen species (ROS) production, and DNA and mitochondrial damage between pretreated and untreated control cells using a range of assays, flow cytometry, and Western blot analysis and also examined the reducing power and DPPH free radical scavenging activity of APOB. Results: APOB pretreatment significantly increased cell viability, effectively attenuated H2O2-induced comet tail formation, and inhibited H2O2-induced phosphorylation of the histone γH2AX, as well as the number of apoptotic bodies and Annexin V-positive cells. APOB was found to have high reducing power and DPPH radical scavenging activity and also exhibited scavenging activity against intracellular ROS accumulation and restored the loss of mitochondrial membrane potential caused by H2O2. APOB pretreatment almost totally reversed the enhanced cleavage of caspase-3, the degradation of poly (ADP-ribose)-polymerase (PARP), DNA fragmentation that usually occurs in the presence of H2O2, and increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme that is associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). Conclusions: APOB can protect HaCaT cells from H2O2-induced DNA damage and cell death by blocking cellular damage related to oxidative stress via a mechanism that affects ROS elimination and by activating the Nrf2/HO-1 signaling pathway.

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Nanotechnology for Natural Medicine: Formulation of Neem Oil Loaded Phospholipid Vesicles Modified with Argan Oil as a Strategy to Protect the Skin from Oxidative Stress and Promote Wound Healing

Antioxidants ◽

10.3390/antiox10050670 ◽

2021 ◽

Vol 10 (5) ◽

pp. 670

Author(s):

Maria Letizia Manca ◽

Maria Manconi ◽

Maria Cristina Meloni ◽

Francesca Marongiu ◽

Mohamad Allaw ◽

...

Keyword(s):

Oxidative Stress ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Sodium Hyaluronate ◽

In Vitro Studies ◽

Protective Effects ◽

Neem Oil ◽

Natural Medicine ◽

Argan Oil

Neem oil, a plant-derived product rich in bioactives, has been incorporated in liposomes and hyalurosomes modified by adding argan oil and so called argan-liposomes and argan-hyalurosomes. Argan oil has also been added to the vesicles because of its regenerative and protective effects on skin. In the light of this, vesicles were specifically tailored to protect the skin from oxidative stress and treat lesions. Argan-liposomes were the smallest vesicles (~113 nm); the addition of sodium hyaluronate led to an increase in vesicle size (~143 nm) but it significantly improved vesicle stability during storage. In vitro studies confirmed the free radical scavenging activity of formulations, irrespective of their composition. Moreover, rheological investigation confirmed the higher viscosity of argan-hyalurosomes, which avoid formulation leakage after application. In vitro studies performed by using the most representative cells of the skin (i.e., keratinocytes and fibroblasts) underlined the ability of vesicles, especially argan-liposomes and argan-hyalurosomes, to counteract oxidative stress induced in these cells by using hydrogen peroxide and to improve the proliferation and migration of cells ensuring the more rapid and even complete closure of the wound (scratch assay).

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Protective Effects of Oenothera Biennis Against Hydrogen Peroxide-Induced Oxidative Stress and Cell Death in Skin Keratinocytes

10.20944/preprints202009.0194.v1 ◽

2020 ◽

Author(s):

Seung Young Lee ◽

Chul Hwan Kim ◽

Buyng Su Hwang ◽

Kyung-Min Choi ◽

In-Jun Yang ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Death ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Hacat Cells ◽

Scavenging Activity ◽

Protective Effects ◽

Oenothera Biennis ◽

Skin Keratinocytes

Background: Oenothera biennis (evening primrose) produces bioactive substances with a diverse range of pharmacological functions. However, it is currently unknown whether extract prepared from the aerial parts of O. biennis (APOB) can protect the skin against oxidative stress. To investigate the protective effects of APOB against oxidative stress-induced damage in human skin keratinocytes (HaCaT) and elucidate the underlying mechanisms. Methods: We pretreated HaCaT cells with various concentrations of APOB or the antioxidant N-acetyl-L-cysteine before applying H2O2. We then compared the cell viability, intracellular reactive oxygen species (ROS) production, and DNA and mitochondrial damage between pretreated and untreated control cells using a range of assays, flow cytometry, and Western blot analysis and also examined the reducing power and DPPH free radical-scavenging activity of APOB. Results: APOB pretreatment significantly increased cell viability, effectively attenuated H2O2-induced comet tail formation, and inhibited H2O2-induced phosphorylation of the histone γH2AX, as well as the number of apoptotic bodies and Annexin V-positive cells. APOB was found to have a high reducing power and DPPH radical-scavenging activity and also exhibited scavenging activity against intracellular ROS accumulation and restored the loss of mitochondrial membrane potential caused by H2O2. APOB pretreatment almost totally reversed the enhanced cleavage of caspase-3, the degradation of poly (ADP-ribose)-polymerase (PARP), DNA fragmentation that usually occurs in the presence of H2O2 and increased the levels of heme oxygenase-1 (HO-1), a potent antioxidant enzyme that is associated with the induction of nuclear factor-erythroid 2-related factor 2 (Nrf2). Conclusions: APOB can protect HaCaT cells from H2O2-induced DNA damage and cell death by blocking cellular damage related to oxidative stress via a mechanism that affects ROS elimination and by activating the Nrf2/HO-1 signaling pathway.

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