In Vitro Antioxidant Activities of Polysaccharides from Auricularia auriada

This study aims to evaluate the antioxidative activities of water and alkaline solution pH=8, 50% ethanolic (APP1 or SAPP1), 75% ethanolic (APP2 or SAPP2) and 100% ethanolic (APP3 or SAPP3) extracts of A. auricular. The antioxidant activities, including the ABTS, superoxide anion radical-scavenging effect, hydrogen peroxide radical-scavenging effect and chelating ability on ferrous ions were studied in vitro. A comparison of the 50% effective concentration (EC50) values of different antioxidative reactions revealed that SAPP3 was more effective in scavenging ABTS, hydrogen peroxide and chelating ability on ferrous ions.SAPP2 showed a higher superoxide radical-scavenging activity. The alkali-soluble polysaccharides extracts showed higher radical-scavenging effect than water-soluble polysaccharides.

Download Full-text

Synthesis and characterization of new 4-aryl-2-(2-oxopropoxy)-6-(2,5-dichlorothiophene)nicotinonitrile and their furo[2,3-b]pyridine derivatives: Assessment of antioxidant and biological activity

European Journal of Chemistry ◽

10.5155/eurjchem.9.4.375-381.1792 ◽

2018 ◽

Vol 9 (4) ◽

pp. 375-381 ◽

Cited By ~ 3

Author(s):

Mahmoud Al‐Refai ◽

Mohammad Ibrahim ◽

Abdullah Al‐Fawwaz ◽

Armin Geyer

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

2D Nmr ◽

Pyridine Derivatives ◽

Esi Ms ◽

Scavenging Effect ◽

Radical Scavenging Effect ◽

New Compounds

A new series of furo[2,3-b]pyridine derivatives bearing aryl substituents were synthesized in two steps, where, the cyano-(2H)-pyridones (1a-l) were converted to the corresponding nicotinonitriles (2a-l), followed by the Thorpe-Ziegler ring cyclization to the furo[2,3-b]pyridine derivatives (3a-l). All new compounds were characterized by 1D-NMR experiments (1H and 13C) and 2D-NMR experiments (COSY, HMBC and HSQC), as well as ESI-MS and HR-ESI-MS data. The new compounds were screened for their antioxidant activities by 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) free radical assay. The highest radical scavenging effect was observed for nicotinonitriles 2d, 2h and 2l and furo[2,3-b]pyridines 3b, 3f and 3j by methanolic solvent at 4.0 mg/mL concentration. Remarkably, all nicotinonitriles and furo[2,3-b]pyridine exhibited a significant radical scavenging activity after 24 and 48 hours compared with 0.5 hour.

Download Full-text

Antioxidant and Protective Effect of Ethyl Acetate Extract ofPodophyllum hexandrumRhizome on Carbon Tetrachloride Induced Rat Liver Injury

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/238020 ◽

2011 ◽

Vol 2011 ◽

pp. 1-12 ◽

Cited By ~ 20

Author(s):

Showkat Ahmad Ganie ◽

Ehtishamul Haq ◽

Akbar Masood ◽

Abid Hamid ◽

Mohmmad Afzal Zargar

Keyword(s):

Carbon Tetrachloride ◽

Protective Effect ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Peroxide Radical ◽

Scavenging Activity

The antioxidant and hepatoprotective activities of ethyl acetate extract was carefully investigated by the methods of DPPH radical scavenging activity, Hydroxyl radical scavenging activity, superoxide radical scavenging activity, hydrogen peroxide radical scavenging activity, and its reducing power ability. All thesein vitroantioxidant activities were concentration dependent, which were compared with standard antioxidants such as BHT, α-tocopherol. The hepatoprotective potential ofPodophyllum hexandrumextract was also evaluated in male Wistar rats against carbon tetrachloride- (CCl4-) induced liver damage. Pretreated rats were given ethyl acetate extract at 20, 30, and 50 mg/kg dose prior to CCl4administration (1 mL/kg, 1:1 in olive oil). Rats pretreated withP. hexandrumextract remarkably prevented the elevation of serum AST, ALT, LDH, and liver lipid peroxides in CCl4-treated rats. Hepatic glutathione levels were significantly increased by the treatment with the extract in all the experimental groups. The extract at the tested doses also restored the levels of liver homogenate enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase, and glutathione-S-transferase) significantly. This study suggests that ethyl acetate extract ofP. hexandrumhas a liver-protective effect against CCl4-induced hepatotoxicity and possessin vitroantioxidant activities.

Download Full-text

Phytochemically evaluation and net anti-oxidant activity of Tunisian Melia azedarach leaves extract from their ProAntidex parameter

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i2.25981 ◽

2016 ◽

Vol 11 (2) ◽

pp. 301 ◽

Cited By ~ 1

Author(s):

Maroua Akacha ◽

Karima Lahbib ◽

Néziha Ghanem Boughanmi

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Bioactive Molecules ◽

Ferrous Ion ◽

Melia Azedarach ◽

Chelating Ability

Phytotherapy is a discipline which is interested in the design, the preparation and the interpretation of structure activity relationship of the natural bioactive molecules. In this context, ethanolic leaves extract of Melia azedarach L. was phytochemically analysed on the bases of HPLC and by GC–MS. Extract wase tested for his in vitro antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH), H2O2, hydroxyl radical scavenging activity, Ferric Reducing Power (FRP) and Ferrous ion chelating abilities methods. The antioxidant activity of the extract was analyzed simultaneously with their pro-oxidant capacity. The ratio of pro-oxidant to the antioxidant activity (ProAntidex) represents a useful index of the net free radical scavenging potential of the synthesized compounds. Tested extract showed significant antioxidant activity with a moderate ProAntidex.Video clips:<a href="https://youtube.com/v/FkqJ-qAoyNI">Ferrous ion chelating ability (FIC) 1</a><a href="https://youtube.com/v/LiZIYq54-ms">Ferrous ion chelating ability (FIC) 2</a><a href="https://youtube.com/v/dTNWTEQf8Hs">Ferrous ion chelating ability (FIC) 3</a>

Download Full-text

Radical Scavenging Activities of Novel Cationic Inulin Derivatives

Polymers ◽

10.3390/polym10121295 ◽

2018 ◽

Vol 10 (12) ◽

pp. 1295

Author(s):

Yuan Chen ◽

Yingqi Mi ◽

Jingjing Zhang ◽

Fang Dong ◽

Qing Li ◽

...

Keyword(s):

Hydroxyl Radical ◽

Hydroxyl Radicals ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Synthesis Process ◽

Scavenging Activity ◽

Scavenging Effect ◽

Radical Scavenging Effect ◽

Ft Ir

Many saccharides are attractive targets for biomaterial applications, due to their abundance, biocompatibility, and biodegradability. In this article, a synthesis process of 6-N-substituted cationic inulin derivatives, including 6-pyridyl-6-deoxyinulin bromide (PIL), 6-(2-amino-pyridyl)-6-deoxyinulin bromide (2APIL), 6-(3-amino-pyridyl)-6-deoxyinulin bromide (3APIL), 6-(4-amino-pyridyl)-6-deoxyinulin bromide (4APIL), 6-(2,3-diamino-pyridyl)-6-deoxyinulin bromide (2,3DAPIL), 6-(3,4-diamino-pyridyl)-6-deoxyinulin bromide (3,4DAPIL), and 6-(2,6-diamino-pyridyl)-6-deoxyinulin bromide (2,6DAPIL) was described. The C6-OH of inulin was first activated by PPh3/N-bromosuccinimide (NBS) bromination. Then, pyridine and different kinds of amino-pyridine groups (different position and different numbers of amino) were grafted onto inulin, respectively, via nucleophilic substitution. Then, we confirmed their structure by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy. After this, their radical scavenging activities against hydroxyl radical and diphenylpicryl phenylhydrazine (DPPH) radical were tested in vitro. Each derivative showed a distinct improvement in radical scavenging activity when compared to inulin. The hydroxyl-radical scavenging effect decreased in the following order: 3APIL > PIL > 3,4DAPIL > 4APIL > 2,3DAPIL > 2,6DAPIL > 2APIL. Amongst them, 3APIL revealed the most powerful scavenging effect on hydroxyl radicals, as well as DPPH radicals. At 1.6 mg/mL, it could completely eliminate hydroxyl radicals and could clear 65% of DPPH radicals. The results also showed that the steric hindrance effect and the substitute position of the amino group had an effect on the radical scavenging activity. Moreover, the application prospects of inulin derivatives as natural antioxidant biomaterials are scientifically proven in this paper.

Download Full-text

Synthesis of Novel Nitric Oxide Releasing Furoxan Hybrids from Biologically Active Bicyclic Amine and their Antioxidant Activity

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.21948 ◽

2019 ◽

Vol 31 (8) ◽

pp. 1724-1728

Author(s):

P.M. Swami ◽

P.K. Zubaidha ◽

G.B. Tiwari

Keyword(s):

Nitric Oxide ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Biologically Active ◽

Scavenging Effect ◽

Antioxidant Agents ◽

Radical Scavenging Effect ◽

Nitric Oxide Releasing

The present paper describes the synthesis of novel nitric oxide hybrids obtained by linking bioactive bicyclic amine to substituted furoxans. The antioxidant activities were studied in vitro based on the radical scavenging effect of stable DPPH free radical using ascorbic acid as a standard. The nitric oxide hybrids showed remarkable antioxidant properties and hence, can be employed as potential antioxidant agents.

Download Full-text

Polysaccharide-Rich Fractions from Rosa rugosa Thunb.—Composition and Chemopreventive Potential

Molecules ◽

10.3390/molecules24071354 ◽

2019 ◽

Vol 24 (7) ◽

pp. 1354 ◽

Cited By ~ 6

Author(s):

Marta Olech ◽

Natalia Nowacka-Jechalke ◽

Maciej Masłyk ◽

Aleksandra Martyna ◽

Wioleta Pietrzak ◽

...

Keyword(s):

Radical Scavenging ◽

Water Soluble ◽

In Vitro Assays ◽

Rosa Rugosa ◽

Chemopreventive Agents ◽

Scavenging Effect ◽

Cellular Processes ◽

Colon Cancer Cell Lines ◽

Radical Scavenging Effect

The huge health-beneficial potential of polysaccharides encourages the search for novel sources and applications of these compounds. One poorly explored source of polysaccharides is the rose. The content and biological activity of polysaccharides in rose organs is an almost completely unaddressed topic, therefore, polysaccharide-rich extracts (crude polysaccharides, CPLs) from petals, leaves, hips, and achenes of Rosa rugosa Thunb. were studied for their composition and the influence on various cellular processes involved in the development of cancer and other civilization diseases. The study revealed the presence of water-soluble and -insoluble polysaccharides (including β-glucans) and protein-polysaccharide conjugates in rose organs. Rose hips were found to be the most abundant source of polysaccharides. Different polysaccharide-rich extracts showed the ability to inhibit pro-inflammatory enzymes (COX-1, COX-2, hyaluronidase), a radical scavenging effect (against DPPH• and ABTS•+), and antiproliferative activity (in the A549 lung and SW480 colon cancer cell lines) in in vitro assays. Therefore, rose crude polysaccharides are very promising and can potentially be used as natural chemopreventive agents.

Download Full-text

In Vitro Antioxidant Activity of Electrospun Chitosan Aqueous Salts Based Nanofiber Mats

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1060.111 ◽

2014 ◽

Vol 1060 ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

Natthan Charernsriwilaiwat ◽

Theerasak Rojanarata ◽

Tanasait Ngawhirunpat ◽

Praneet Opanasopit

Keyword(s):

Metal Ion ◽

Ethylenediaminetetraacetic Acid ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Thiamine Pyrophosphate ◽

Scavenging Effect ◽

Radical Scavenging Effect ◽

Nanofiber Mats ◽

Salt Forms

The aim of this study was to investigate the antioxidant activities of chitosan acetate (CS-acetate), chitosan hydroxybenzotriazole (CS-HOBt), chitosan thiamine pyrophosphate (CS-TPP) and chitosan ethylenediaminetetraacetic acid (CS-EDTA) nanofiber mats. Chitosan was dissolved with hydroxybenzotriazole (HOBt), thiamine pyrophosphate (TPP) and ethylenediaminetetraacetic acid (EDTA) in distilled water. These chitosan aqueous salts were blended with polyvinyl alcohol (PVA) at volume ratio 30/70 chitosan salts/PVA and prepared to nanofibers via electrospinning process. The morphology of electrospun chitosan aqueous salts based nanofiber mats were observed under scanning electron microscope (SEM). The antioxidant activities were determined employing various established in vitro system such as superoxide, hydroxyl radicals scavenging and metal ion chelating compared with pure PVA nanofiber mats. The results exhibited that the electrospun chitosan aqueous salts based nanofiber mats showed the different antioxidant activity depended on salt forms. Among the salt forms, CS-acetate nanofiber mats showed the highest superoxide radical scavenging effect while CS-HOBt nanofiber mats (IC50 = 7.53 mg/mL) showed the highest hydroxyl radical scavenging effect. For the metal ion chelating activity, CS-EDTA nanofiber mats showed the highest chelating activity (IC50 = 1.07 mg/mL). In summary, the antioxidant chitosan aqueous salt based nanofiber mats have potential for use in pharmaceutical applications.

Download Full-text

Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

Evidence-based Complementary and Alternative Medicine ◽

10.1093/ecam/nem098 ◽

2009 ◽

Vol 6 (2) ◽

pp. 227-231 ◽

Cited By ~ 28

Author(s):

S. A. Adesegun ◽

A. Fajana ◽

C. I. Orabueze ◽

H. A. B. Coker

Keyword(s):

Ascorbic Acid ◽

Leaf Extract ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

Download Full-text

Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

Croatian Journal of Food Science and Technology ◽

10.17508/cjfst.2020.12.2.05 ◽

2020 ◽

Vol 12 (2) ◽

pp. 177-183

Author(s):

Idorenyin Nwaehujor ◽

Samuel Akande ◽

Olubunmi Atolani ◽

Gabriel Olatunji

Keyword(s):

Hydrogen Peroxide ◽

Cell Membrane ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanol Extract ◽

Scavenging Activity ◽

Membrane Stabilization ◽

Anti Inflammatory ◽

Proteinase Inhibition

Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

Download Full-text

Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

International Journal of Phytomedicine ◽

10.5138/09750185.2123 ◽

2017 ◽

Vol 9 (4) ◽

pp. 615

Author(s):

Mukesh Kumar Yadav ◽

Santosh Kumar Singh ◽

JS Tripathi ◽

YB Tripathi

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Centella Asiatica ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Phytochemical Screening

Centella asiatica also known as mandukparni or Indian pennywort or jalbrahmi, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. Centella asiatica has long been used to improve memory and cognitive function.The study aimed to identify the phytochemicals present in different solvent extracts of Centella asiatica (i.e. PECA- Petroleum ether extract of C. asiatica, CCA- Chloroform extract of C. asiatica, EACA- Ethyl acetate extract of C. asiatica, ECA- Ethanolic extract of C. asiatica, HACA- Hydro-alcoholic extract of C. asiatica) and evaluate the respective in-vitro antioxidant potentials. The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of Centella asiatica was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.In all different solvent extracts of C. asiatica revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with EC50 values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC50 values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity. The present study indicates that the Centella asiatica extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.

Download Full-text