scholarly journals Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo

2019 ◽  
Vol 2019 ◽  
pp. 1-4 ◽  
Author(s):  
Mas Athira Johari ◽  
Heng Yen Khong
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Gram Positive Bacteria ◽  
Antioxidant Agents ◽  
Hexane Extracts ◽  
Dpph Scavenging

Different solvent extracts of Pereskia bleo leaves were evaluated for total phenolic content (TPC) and antioxidant activities based on the Folin–Ciocalteu test and DPPH scavenging activities. The antibacterial activities against four bacteria, namely, Gram-positive bacteria: Streptococcus pyogenes ATCC 19615 (SP) and Staphylococcus aureus ATCC 29737 (SA) and Gram-negative bacteria: Escherichia coli ATCC 10536 (EC) and Pseudomonas aeruginosa ATCC 9027 (PA), were also performed based on the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. The findings demonstrated that both the methanolic and chloroform extracts displayed strong activities against SA, SP, EC, and PA while the hexane extract demonstrated the weakest activities towards all the four bacteria. The methanolic extract also exhibited higher TPC and possessed higher antioxidant activity with the IC50 value 33.83 µg/mL compared to the chloroform and hexane extracts. As such, the methanolic extract has a higher ability to scavenge free radical compared to other extracts. Due to the interesting result, activities are shown by the methanolic and chloroform crude extracts of P. bleo; hence, the study has been extended to the isolation of bioactive compounds to uncover its great potential as a natural source for antibacterial and antioxidant agents.

Optimization of Total Phenol and Tannin Content and Biological Activity of Dittrichia graveolens (L.) GREUTER.

2020 ◽  
Vol 16 (2) ◽  
pp. 124-132 ◽  
Author(s):  
Mahsa Souri ◽  
Alireza Shakeri
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antibacterial Activities ◽  
Methanol Concentration ◽  
Total Phenolic ◽  
Tannin Content ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Background: Dittrichia graveolens (L.) is a strongly aromatic plant distributed in Mediterranean regions. This research concerns the optimization of the Total Phenolic Content (TPC) and the Total Tannin Content (TTC) of Dittrichia graveolens (L.) extracts using Response Surface Methodology for Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). Central Composite Design (CCD) was used to evaluate the effect of the solvent concentration and the extraction time, in different methods on TTC and TPC. The antioxidant activities and antibacterial activities were evaluated. Methods: The plant extracts were prepared using maceration, microwave and ultrasound assisted extraction. TPC and TTC were measured using Folin-Ciocalteu method. The antioxidant activities were studied using DPPH reagent and disc diffusion method was used to study the antibacterial activities. Results: This study showed the optimum condition for UAE was 49.96% methanol concentration and 11.2 min sonication, while for MAE was 55.44% methanol concentration and 2.26 min microwave extraction. It also indicated that MAE was the most effective method in comparison to UAE and maceration. The antioxidant activities of MAE extract (IC50=7.7mg/mL) were more than UAE extract (IC50=21.5mg/mL) and maceration (IC50=32.3mg/mL). Conclusion: As a conclusion, it was indicated that MAE was the most effective method. The higher total phenolic content caused higher antioxidant activities as MAE extract had the highest antioxidant activities. The antibacterial test showed the great potential of this plant as an antibacterial compound resource against different bacteria.

scholarly journals Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

2021 ◽  
Author(s):  
Bing Zhou ◽  
Zhao Jin ◽  
Paul B. Schwarz ◽  
Yin Li
Keyword(s):  
Phenolic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

scholarly journals Sorghum halepense (L.) Pers rhizomes inhibitory potential against diabetes and free radicals

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Muhammad Abdur Rehman Shah ◽  
Rahmat Ali Khan ◽  
Mushtaq Ahmed
Keyword(s):  
Free Radicals ◽  
Total Phenolic Content ◽  
Cardiac Glycosides ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Sorghum Halepense ◽  
Total Phenolic ◽  
Brine Shrimps

Abstract Background Owing to the side effects of synthetic medicine and less effectiveness against different syndromes, the researchers have focused on phytotherapy to overcome these problems. The purpose of this project was to study the in vitro phytochemical, cytotoxic, total phenolic, antioxidant and antidiabetic activities of the methanol extract of the rhizome of Sorghum halepense (L.) Pers and its n-hexane, chloroform and aqueous fractions. Thereafter, to conduct in vivo evaluation of the effective extract for its antidiabetic and antioxidant characteristics. Methods Cytotoxic, total phenolic content and antidiabetic properties were ascertained by brine shrimps lethality, Folin- Ciocalteu reagent and alpha-amylase inhibition assays respectively while antioxidant activities were investigated through DPPH, ABTS and H2O2 assays. The methanolic extract was assessed in vivo for its antidiabetic and antioxidant activities by using Wistar albino rats. Results The phytochemical investigation of the methanolic extract and its unlike fractions revealed the availability of alkaloids, cardiac glycosides, flavonoids, terpenes, steroids, carbohydrate and proteins while lack of saponins and gums in methanolic extract. Steroids and carbohydrates were only present in aqueous and chloroform fraction respectively while both fractions contained proteins and alkaloids. Cardiac glycosides and flavonoids were absent in aqueous and chloroform fractions respectively. The highest brine shrimps lethality (70.5 ± 1.2), total phenolic content (28.30 ± 1.3 mg GAE/g), free radicals scavenging potential i.e. DPPH (40.02%), ABTS (40.48%) and H2O2 (50.85%) and alpha amylase inhibition (61.87%) was shown by the methanolic extract. The in vivo results did not disclose any sign of acute toxicity. The diabetic control showed a noteworthy (P < 0.05) decline in weight, HDL and glutathione and a raised level of bilirubin, blood glucose, urea, creatinine, triglyceride, LDL, VLDL, ALT, ALP, AST, SOD, catalase. The mentioned alterations were restored considerably (P < 0.05) by treatment of diabetic rats with methanolic extract of Sorghum halepense (L.) Pers (150 and 300 mg/kg b.w.). Conclusion It is concluded that the extract of rhizomes of Sorghum halepense (L.) Pers is an effective fount of antioxidant and anti-diabetic compounds. Further analysis is needed to sharpen its pharmacological activities.

scholarly journals Pharmacognostic evaluation, estimation of phenolic, ϐlavonoid composition and antioxidant activity of Aspidopterys indica (Willd.)W.Theob: An endemic plant to peninsular India

2021 ◽  
Vol 12 (2) ◽  
pp. 1223-1232
Author(s):  
Udaya Chandrika P ◽  
Sunitha K
Keyword(s):  
Aqueous Extract ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Endemic Plant ◽  
Peninsular India ◽  
Crude Fiber Content ◽  
Dpph Scavenging

Aspidopterys indica (Willd.)W.Theob is endemic plant to Peninsular India belonging to family Malpighiaceae commonly known as  Chuttakulaa-tigga. The study aims to explore Pharmacognostic, Phytochemical evaluation, Total Phenolic, Flavonoid Content and Antioxidant Activities.The Pharmacognostic study comprises of Ultrasonic extraction, microscopy of Leaf and stem, fluorescence investigation, ash values, extractive values, Loss on drying, foaming index, crude fiber content, total phenolic and flavonoid content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and Nitric oxide scavenging activities were assessed.The leaf shows presence of paracytic stomata, T shaped trichomes, Stem shows presence of vessels and pith. The physicochemical characters assessed as Total Ash value 10%, Water insoluble ash 0.5%, acid insoluble ash 8.5%, sulphated ash 4.2%, alcoholic extractive value 16.8%, water soluble extractive value 17.6%, chloroform extractive value 0.8%, Loss on drying 3.36%, Foaming index is <100, crude fiber content is 2.4%, phytoconstituents like tannins, flavonoids, phytosterols and alkaloids, carbohydrates are available in methanol, aqueous, chloroform extracts. Total phenolic content turned greatest in methanolic extract (90.69±2.86 mg GAE/g), Aqueous extract showed maximum total flavonoid content (109.89±0.33mg of QE/g). Methanolic extract indicated most intense 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging action IC50(134.97±8.37) whereas aqueous extract is potent to exclude nitrile radical IC50108.00±4.18). Total phenolic content - DPPH scavenging assay are positively correlated. The microscopical, physicochemical parameters are valuable to authenticate the identification and purity of the drug. A.indica is a perspective source of antioxidants.

Phytochemical Screening, Antioxidant Activity and Total Phenolic Content of Spirogyra spp.

2013 ◽  
Vol 699 ◽  
pp. 693-697 ◽  
Author(s):  
Rattanabhorn Junthip ◽  
Doungporn Amornlerdpison ◽  
Thitiphan Chimsook
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Rapid Test ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Abts Assay

A freshwater macroalga, Spirogyra spp., were analyzed for its phytochemical composition, antioxidant activity and total phenolic content (Folin−Ciocalteu method). Phytochemical analysis of Spirogyra neglecta revealed presence of phenolics, tannins, glycosides and saponins. The crude extraction of Spirogyra spp. was carried out using two solvents via, methanol (methanolic extract) and water (aqueous extract). The total phenolic contents of crude extracts were shown at 346.58±1.61 and 589.77±1.65 mg gallic acid equivalents/g extract in aqueous and methanolic extracts, respectively. The antioxidant capacity of aqueous and methanolic extract was estimated by two different methods; ABTS assay, and DPPH assay. The antioxidant activity of two extracts is affected by the extracting solvent and different assay. In the DPPH scavenging assay and ABTS assay, both extracts showed high antioxidant activity. In addition, the high correlation between results of all antioxidant activities and total phenolic content was found. The rapid TLC assay in methanolic extract is considered as the rapid test to evaluate the antioxidant activity of natural compounds. The compounds showing four bands at Rf = 0.25, 0.35, 0.42, 0.64. This study showed that Spirogyra spp. might constitute an important source of natural antioxidants.

scholarly journals Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Sani N. Fitriansyah ◽  
Irda Fidrianny ◽  
Komar Ruslan
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Sesbania Sesban ◽  
Total Carotenoid Content ◽  
Dpph Method ◽  
Dpph Scavenging

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl.

Holzforschung ◽  
2006 ◽  
Vol 60 (4) ◽  
pp. 459-462 ◽  
Author(s):  
Heng Gao ◽  
Todd F. Shupe ◽  
Chung Y. Hse ◽  
Thomas L. Eberhardt
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Hexane Extracts ◽  
Chamaecyparis Lawsoniana

Abstract The bark of Chamaecyparis lawsoniana (A. Murray) Parl. was extracted with methanol and sequentially partitioned with n-hexane, ethyl acetate, n-butanol and deionized water. The antioxidant activities of the four extracts were evaluated using the DPPH• and ABTS+• methods. The total phenolic content of the extracts was determined using the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). Butylated hydroxytoluene was used as a positive control in the radical-scavenging activity tests. All the bark extracts showed significant radical-scavenging activity. In the ABTS+• assay, n-butanol extracts exhibited the strongest radical-scavenging activity, followed by ethyl acetate, water, and n-hexane extracts. The greatest total phenolic content was 428.54 mg GAE per gram of dry extract and was detected in the n-butanol extract, followed by the ethyl acetate and n-hexane extracts. The antioxidant activities correlate with the amount of phenolics present in these extracts. The ethyl acetate and n-butanol extracts were rich in phenolics and may represent a good source of antioxidants.

Comparative Study of Antioxidant Activities and Total Phenolic Content of Selected Edible Wild Mushrooms

2011 ◽  
Vol 13 (3) ◽  
pp. 245-255 ◽  
Author(s):  
Hip Seng Yim ◽  
Fook Yee Chye ◽  
Mee Yee Lee ◽  
Patricia Matanjun ◽  
Siew Eng How ◽  
...  
Keyword(s):  
Comparative Study ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Wild Mushrooms ◽  
Edible Wild Mushrooms

scholarly journals Oenothera laciniata Hill Extracts Exhibits Antioxidant Effects and Attenuates Melanogenesis in B16-F10 Cells via Downregulating CREB/MITF/Tyrosinase and Upregulating p-ERK and p-JNK

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 727
Author(s):  
Horng-Huey Ko ◽  
Yeo-Tzu Chang ◽  
Yueh-Hsiung Kuo ◽  
Chia-Hsuan Lin ◽  
Yih-Fung Chen
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Tyrosinase Activity ◽  
Perennial Herb ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Protective Agent

Oenothera laciniata Hill is a perennial herb traditionally used to alleviate inflammatory complications. This study investigated the antioxidant and anti-melanogenic activities of O. laciniata. The methanolic extract (OLM) of O. laciniata and its different fractions, including ethyl acetate (OLEF), n-butanol (OLBF), and water (OLWF) fractions, were prepared. Antioxidant activities were evaluated by total phenolic content, the radical-scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH•), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+•), and superoxide anion (O2−•), reducing capacity, and metal chelating ability. OLM and its fractions exhibited potent antioxidant activity in these in vitro assays, with a correlation between radical-scavenging activity and total phenolic content. OLM and its fractions inhibited the mushroom tyrosinase activity superior to the reference control, ascorbic acid. In B16-F10 melanoma cells, OLM and its fractions significantly decreased melanin production and tyrosinase activity. Mechanistic investigations revealed that OLM and its fractions inhibited tyrosinase and TRP-2 expressions via downregulating MITF and phosphorylated CREB and differentially inducing ERK or JNK phosphorylation. Additionally, OLM and its fractions caused no significant cytotoxicity towards B16-F10 or skin fibroblast cells at concentrations used in these cellular assays. These findings demonstrated the potential of O. laciniata extracts as the ideal skin protective agent with dual antioxidant and anti-melanogenic activities.

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