Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study

Polycaprolactone (PCL) has attracted great attention for bone regeneration attributed to its cost-efficiency, high toughness, and good processability. However, the relatively low elastic modulus, hydrophobic nature, and insufficient bioactivity of pure PCL limited its wider application for bone regeneration. In the present study, the effects of the addition of boron containing bioactive glass (B-BG) materials on the mechanical properties and biological performance of PCL polymer were investigated with different B-BG contents (0, 10, 20, 30, and 40 wt.%), in order to evaluate the potential applications of B-BG/PCL composites for bone regeneration. The results showed that the B-BG/PCL composites possess better tensile strength, human neutral pH value, and fast degradation as compared to pure PCL polymers. Moreover, the incorporation of B-BG could enhance proliferation, osteogenic differentiation, and angiogenic factor expression for rat bone marrow stromal cells (rBMSCs) as compared to pure PCL polymers. Importantly, the B-BG also promoted the angiogenic differentiation for human umbilical vein endothelial cells (HUVECs). These enhanced effects had a concentration dependence of B-BG content, while 30 wt.% B-BG/PCL composites achieved the greatest stimulatory effect. Therefore the 30 wt.% B-BG/PCL composites have potential applications in bone reconstruction fields.

Download Full-text

Effect of TNFα stimulation on expression of kidney risk inflammatory proteins in human umbilical vein endothelial cells cultured in hyperglycemia

Scientific Reports ◽

10.1038/s41598-021-90496-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zaipul I. Md Dom ◽

Caterina Pipino ◽

Bozena Krolewski ◽

Kristina O’Neil ◽

Eiichiro Satake ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Systemic Exposure ◽

In Vitro Study ◽

Human Umbilical Vein ◽

Intracellular Fraction ◽

Inflammatory Proteins ◽

Umbilical Vein Endothelial Cells ◽

Extracellular Fraction

AbstractWe recently identified a kidney risk inflammatory signature (KRIS), comprising 6 TNF receptors (including TNFR1 and TNFR2) and 11 inflammatory proteins. Elevated levels of these proteins in circulation were strongly associated with risk of the development of end-stage kidney disease (ESKD) during 10-year follow-up. It has been hypothesized that elevated levels of these proteins in circulation might reflect (be markers of) systemic exposure to TNFα. In this in vitro study, we examined intracellular and extracellular levels of these proteins in human umbilical vein endothelial cells (HUVECs) exposed to TNFα in the presence of hyperglycemia. KRIS proteins as well as 1300 other proteins were measured using the SOMAscan proteomics platform. Four KRIS proteins (including TNFR1) were down-regulated and only 1 protein (IL18R1) was up-regulated in the extracellular fraction of TNFα-stimulated HUVECs. In the intracellular fraction, one KRIS protein was down-regulated (CCL14) and 1 protein was up-regulated (IL18R1). The levels of other KRIS proteins were not affected by exposure to TNFα. HUVECs exposed to a hyperglycemic and inflammatory environment also showed significant up-regulation of a distinct set of 53 proteins (mainly in extracellular fraction). In our previous study, circulating levels of these proteins were not associated with progression to ESKD in diabetes.

Download Full-text

Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds

Journal of The Royal Society Interface ◽

10.1098/rsif.2015.0509 ◽

2015 ◽

Vol 12 (110) ◽

pp. 20150509 ◽

Cited By ~ 13

Author(s):

J. P. Cattalini ◽

A. Hoppe ◽

F. Pishbin ◽

J. Roether ◽

A. R. Boccaccini ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Calcium Release ◽

Umbilical Vein ◽

Burst Release ◽

Potential Applications ◽

Cell Property ◽

Umbilical Vein Endothelial Cells

This work aimed to develop novel composite biomaterials for bone tissue engineering (BTE) made of bioactive glass nanoparticles (Nbg) and alginate cross-linked with Cu 2+ or Ca 2+ (AlgNbgCu, AlgNbgCa, respectively). Two-dimensional scaffolds were prepared and the nanocomposite biomaterials were characterized in terms of morphology, mechanical strength, bioactivity, biodegradability, swelling capacity, release profile of the cross-linking cations and angiogenic properties. It was found that both Cu 2+ and Ca 2+ are released in a controlled and sustained manner with no burst release observed. Finally, in vitro results indicated that the bioactive ions released from both nanocomposite biomaterials were able to stimulate the differentiation of rat bone marrow-derived mesenchymal stem cells towards the osteogenic lineage. In addition, the typical endothelial cell property of forming tubes in Matrigel was observed for human umbilical vein endothelial cells when in contact with the novel biomaterials, particularly AlgNbgCu, which indicates their angiogenic properties. Hence, novel nanocomposite biomaterials made of Nbg and alginate cross-linked with Cu 2+ or Ca 2+ were developed with potential applications for preparation of multifunctional scaffolds for BTE.

Download Full-text

Complement Factor H Expressed by Retinal Pigment Epithelium Cells Can Suppress Neovascularization of Human Umbilical Vein Endothelial Cells: An in vitro Study

PLoS ONE ◽

10.1371/journal.pone.0129945 ◽

2015 ◽

Vol 10 (6) ◽

pp. e0129945 ◽

Cited By ~ 4

Author(s):

Yi Zhang ◽

Qing Huang ◽

Min Tang ◽

Junjun Zhang ◽

Wei Fan

Keyword(s):

Umbilical Vein ◽

Pigment Epithelium ◽

Retinal Pigment ◽

In Vitro Study ◽

Factor H ◽

Complement Factor ◽

Retinal Pigment Epithelium Cells ◽

Umbilical Vein Endothelial Cells ◽

Epithelium Cells

Download Full-text

Gestational hypertension plasma and human umbilical vein endothelial cells: an in vitro study

European Journal of Clinical Investigation ◽

10.1046/j.1365-2362.1998.00398.x ◽

1998 ◽

Vol 28 (12) ◽

pp. 989-993 ◽

Cited By ~ 2

Author(s):

Cester ◽

Salvolini ◽

Rabini ◽

Tranquilli ◽

Zolese ◽

...

Keyword(s):

Endothelial Cells ◽

Gestational Hypertension ◽

Umbilical Vein ◽

In Vitro Study ◽

Vitro Study ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

Download Full-text

Distinct signal transduction pathways are utilized during the tube formation and survival phases of in vitro angiogenesis

Journal of Cell Science ◽

10.1242/jcs.111.24.3621 ◽

1998 ◽

Vol 111 (24) ◽

pp. 3621-3631 ◽

Cited By ~ 6

Author(s):

N. Ilan ◽

S. Mahooti ◽

J.A. Madri

Keyword(s):

Protein Kinase ◽

Umbilical Vein ◽

Tube Formation ◽

Mitogen Activated Protein Kinase ◽

Angiogenic Factor ◽

Collagen Gels ◽

Mitogen Activated Protein ◽

Umbilical Vein Endothelial Cells

Angiogenesis, the formation of new blood vessels from pre-existing ones, occurs during development, wound healing and cancer and involves stages that orchestrate a network of cooperative interactions. Peptide growth factors and extracellular matrix (ECM) components are two major groups of angiogenesis mediators. Among the different ECM proteins, collagens have been well-associated with in vivo angiogenesis. Using human umbilical vein endothelial cells (HUVEC) grown in 3-D collagen gels we show that: (1) HUVEC do not survive well in 3-D collagen gels due to rapid induction of apoptosis. (2) VEGF, a potent in vivo angiogenic factor, fails to induce tube formation. (3) PMA was effective in inducing tube formation and survival in HUVEC dispersed in 3-D collagen gels, activating MAP kinase, phosphoinositide 3-OH kinase (PI-3-kinase) and Akt/PKB (protein kinase B) pathways. (4) VEGF was effective in preventing PMA-induced tube-like structure regression after PMA-withdrawal by (5) activating the mitogen activated protein kinase (MAPK), rather than the Akt/PKB, signaling pathway.

Download Full-text