scholarly journals Metabolomics Analysis of Laparoscopic Surgery Combined with Wuda Granule to Promote Rapid Recovery of Patients with Colorectal Cancer Using UPLC/Q-TOF-MS/MS

2020 ◽  
Vol 2020 ◽  
pp. 1-18
Author(s):  
Tao Wang ◽  
Yihua Xu ◽  
Qicheng Chen ◽  
Weilin Zheng ◽  
Jie Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Laparoscopic Surgery ◽  
Nutritional Status ◽  
Synergistic Effects ◽  
Serum Biochemistry ◽  
Rapid Recovery ◽  
Gastrointestinal Function ◽  
Therapeutic Mechanism ◽  
Metabolomics Analysis ◽  
Tof Ms

Surgery is the primary curative treatment for patients with nonmetastasized colorectal cancer (CRC). Rate of complications, morbidity, mortality, and overall survival of patients with CRC are factors associated with speed of recovery following surgery. Wuda granule (WD) is a traditional Chinese medicine (TCM) prescription used to promote rapid recovery after surgery. However, the specific mechanism of action of WD has not been characterized. Our study included 60 patients with clear histopathological evidence of colon or rectal cancer who underwent CRC laparoscopic surgery and 30 healthy individuals. Serum biochemistry and clinical evaluation of gastrointestinal function showed that WD could improve the nutritional status and gastrointestinal function and reduce the level of inflammation of patients with CRC following laparoscopic surgery. In addition, we used UPLC/Q-TOF-MS/MS-based metabolomics analysis to determine the mechanism of WD-related rapid recovery following laparoscopic surgery in patients with CRC. Twenty metabolites associated with arachidonic acid, alanine, aspartate and glutamate, α-linolenic acid, pyruvate, histidine, and glycerophospholipids were identified. The results suggested that the therapeutic mechanism of laparoscopic surgery combined with WD may be related to regulation of nutritional status, inflammation, immune function, energy, and gastrointestinal function in patients with CRC. This study also highlighted the ability of TCM compounds to interact with multiple targets to induce synergistic effects. This study may result in further studies of WD as a therapeutic agent to promote recovery following surgical resection of CRC tumors.

Outcomes of laparoscopic surgery for pT3/pT4 colorectal cancer in young vs. old patients

2019 ◽  
Vol 74 (4) ◽  
Author(s):  
Gabriele Bellio ◽  
Marina Troian ◽  
Arianna Pasquali ◽  
Nicolò de Manzini
Keyword(s):  
Colorectal Cancer ◽  
Laparoscopic Surgery ◽  
Old Patients

Laparoscopic surgery for palliative resection of the primary tumor in incurable stage IV colorectal cancer

2012 ◽  
Vol 26 (11) ◽  
pp. 3201-3206 ◽  
Author(s):  
Hideaki Nishigori ◽  
Masaaki Ito ◽  
Yuji Nishizawa ◽  
Atsushi Kohyama ◽  
Takamaru Koda ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Laparoscopic Surgery ◽  
Primary Tumor ◽  
Stage Iv ◽  
Palliative Resection ◽  
Stage Iv Colorectal Cancer

scholarly journals Colorectal Cancer Surgery Quality in Manitoba: A Population-Based Descriptive Analysis

2021 ◽  
Vol 28 (3) ◽  
pp. 2239-2247
Author(s):  
Iresha Ratnayake ◽  
Jason Park ◽  
Natalie Biswanger ◽  
Allison Feely ◽  
Grace Musto ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Laparoscopic Surgery ◽  
Descriptive Analysis ◽  
Population Based ◽  
Cancer Surgery ◽  
Formal Structure ◽  
Colorectal Cancer Surgery ◽  
Surgical Quality ◽  
Clinical Variation

Unwarranted clinical variation in healthcare impacts access, productivity, performance, and outcomes. A strategy proposed for reducing unwarranted clinical variation is to ensure that population-based data describing the current state of health care services are available to clinicians and healthcare decision-makers. The objective of this study was to measure variation in colorectal cancer surgical treatment patterns and surgical quality in Manitoba and identify areas for improvement. This descriptive study included individuals aged 20 years or older who were diagnosed with invasive cancer (adenocarcinoma) of the colon or rectum between 1 January 2010 and 31 December 2014. Laparoscopic surgery was higher in colon cancer (24.1%) compared to rectal cancer (13.6%). For colon cancer, the percentage of laparoscopic surgery ranged from 12.9% to 29.2%, with significant differences by regional health authority (RHA) of surgery. In 86.1% of colon cancers, ≥12 lymph nodes were removed. In Manitoba, the negative circumferential resection margin for rectal cancers was 96.9%, and ranged from 96.0% to 100.0% between RHAs. The median time between first colonoscopy and resection was 40 days for individuals with colon cancer. This study showed that high-quality colorectal cancer surgery is being conducted in Manitoba along with some variation and gaps in quality. As a result of this work, a formal structure for ongoing measuring and reporting surgical quality has been established in Manitoba. Quality improvement initiatives have been implemented based on these findings and periodic assessments of colorectal cancer surgery quality will continue.

Laparoscopic surgery for colorectal cancer: safe and effective? – A systematic review

2007 ◽  
Vol 22 (5) ◽  
pp. 1146-1160 ◽  
Author(s):  
Tania Lourenco ◽  
Alison Murray ◽  
Adrian Grant ◽  
Aileen McKinley ◽  
Zygmunt Krukowski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Systematic Review ◽  
Laparoscopic Surgery

scholarly journals Erratum to: A Review of the Long-Term Oncologic Outcomes of Robotic Surgery Versus Laparoscopic Surgery for Colorectal Cancer

2016 ◽  
Vol 78 (3) ◽  
pp. 220-220
Author(s):  
Fatima G. Wilder ◽  
Atuhani Burnett ◽  
Joseph Oliver ◽  
Michael F. Demyen ◽  
Ravi J. Chokshi
Keyword(s):  
Colorectal Cancer ◽  
Laparoscopic Surgery ◽  
Robotic Surgery ◽  
Oncologic Outcomes

Impact of intraoperative hypocapnia on postoperative complications in laparoscopic surgery for colorectal cancer

Surgery Today ◽  
2021 ◽  
Author(s):  
Satoru Morita ◽  
Masashi Tsuruta ◽  
Koji Okabayashi ◽  
Takashi Ishida ◽  
Kohei Shigeta ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Laparoscopic Surgery ◽  
Postoperative Complications

Synergistic Effects of 5-Fluorouracil in Combination with Diosmetin in Colorectal Cancer Cells

2021 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Sareh Kamran ◽  
Ajantha Sinniah ◽  
Mohammed Abdullah Alshawsh
Keyword(s):  
Colorectal Cancer ◽  
Flow Cytometry ◽  
Synergistic Effect ◽  
Cancer Cells ◽  
Synergistic Effects ◽  
Neoadjuvant Treatment ◽  
Software Tool ◽  
New Combination ◽  
Combination Regimen ◽  
Colorectal Cancer Cells

Colorectal cancer (CRC) is among the most commonly occurring cancers. The management of CRC includes laparoscopic surgery, radiotherapy, chemotherapies and neoadjuvant treatment. However, conventional chemotherapies have poor impact on combating CRC and are associated with severe toxic effects and high rates of relapse. Therefore, searching for a new combination regimen is a favorable consideration. The aim of this study was to elucidate the synergistic effect of 5-fluorouracil (5-FU) and diosmetin in an in vitro model on colorectal cancer cells. An MTT assay was conducted on HCT-116 cancer cells and they were treated with a concentration gradient of 5-FU and diosmetin individually and in combination. The combination index (CI) and dose reduction index (DRI) were calculated using CompuSyn software. Isobologram analysis and synergism determination were performed using the Combenefit software tool and the synergy score was calculated using the SynergyFinder 2.0 software tool. The apoptotic features of the cells were determined via an AO/PI double staining assay and an annexin V assay using a fluorescent microscope and the flow cytometry technique, respectively. The findings showed that the DRI of 5-FU was three-fold lower in the combination with a CI value of less than one, which indicates that there was a synergistic effect. The AO/PI microscopic results revealed signs of apoptosis and dead cells after 72 h of treatment. Flow cytometry analysis confirmed that the apoptotic effect of the combination was more prominent compared to 5-FU alone. The findings of this study offer a potential strategy for reducing the cytotoxicity and enhancing the efficacy of 5-FU on colorectal cancer cells through a synergistic study model.

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