scholarly journals Experimental Study on MICP Aqueous Solution under the Action of Different Organic Substrates

Geofluids ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Huai-miao Zheng ◽  
Ling-ling Wu ◽  
Kai-wen Tong ◽  
Lin Hu ◽  
Qing Yu ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Mass Fraction ◽  
Organic Matrix ◽  
Egg White ◽  
Control Group ◽  
Organic Substrates ◽  
Carbonate Formation

The precipitation rate and cementation strength of calcium carbonate crystals during the process of microorganism-induced calcium carbonate precipitation (MICP) are key factors that affect the application effect of this technology. In order to improve the quality of calcium carbonate formation in the MICP process, egg white protein with a volume fraction of 20%, bovine serum albumin with a mass fraction of 0.3%, sucrose with a mass fraction of 5%, bamboo leaves with a mass concentration of 25 g/L, and bamboo leaf-magnesium chloride (Mg2+/Ca2+=4:1) were added during the experiment of different groups of MICP solutions. The results of the solution test study showed that there was no obvious lag period for bacterial growth under the action of organic matrix. The concentration of bacteria in the reaction solution was higher under the action of sucrose and egg white. The conversion rate of Ca2+ under the action of egg white was the fastest, which was about 2.5 times higher than that of the control group. After 14 days of grouting reaction, it was found that the proportion of calcite-type calcium carbonate produced under the action of egg white was the highest, and the Ca element accounted for 66.24% in the solidified material. Sucrose was second; bovine serum albumin was the lowest. The calcium carbonate crystals generated by the control of each organic matrix had a high degree of pore size matching with the tailings under a dry-wet cycle. The structural characteristics of the calcium carbonate crystals, such as crystal form, morphology, and particle size, were mainly due to the interaction between the organic matrix and the calcium carbonate crystals. This study proves that the addition of the organic matrix can improve the formation rate and crystal structure of calcium carbonate during MICP, thus providing a new reference for the development of MICP technology.

Use of Magnetic Fe3O4 Nanoparticles Coated with Bovine Serum Albumin for the Separation OF Lysozyme from Chicken Egg white

2021 ◽  
pp. 129442
Author(s):  
Mariane Gonçalves Santos ◽  
Diailison Teixeira de Carvalho ◽  
Lucas Belga Caminitti ◽  
Bruna Bueno Alves de Lima ◽  
Marcello Henrique da Silva Cavalcanti ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fe3o4 Nanoparticles ◽  
Bovine Serum ◽  
Egg White ◽  
Chicken Egg ◽  
Magnetic Fe3o4 Nanoparticles ◽  
Magnetic Fe3o4

scholarly journals PENGGANTIAN BOVINE SERUM ALBUMIN PADA PENGENCER CEP-2 DENGAN SERUM DARAH SAPI DAN PUTIH TELUR TERHADAP KUALITAS SEMEN CAIR SAPI LIMOUSIN SELAMA PENDINGINAN (The Substitution of Bovine Serum Albumin with Cattle Blood Serum and Egg White in CEP-2 Diluent on the Quality of Limousin Bull Liquid Semen during Refrigerating)

2016 ◽  
Vol 10 (2) ◽  
pp. 98-102
Author(s):  
Trinil Susilawati ◽  
Feri Eka Wahyudi ◽  
Inna Anggraeni ◽  
Nurul Isnaini ◽  
Muhammad Nur Ihsan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Serum ◽  
Sperm Motility ◽  
Bovine Serum ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Egg White ◽  
Cattle Blood

This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.This study aims to determine the effect of the substitution of bovine serum albumin (BSA) with cattle blood serum and egg white in the diluent of Cauda epididymal Plasma 2 (CEP-2) on sperm quality of Limousin cattle during cooling at 3-5 C. The research material used was rejected Limousin bull sperm (motility of 50-60%) from Artificial Insemination Centre Singosari, Malang. This research was a laboratory experiment using a randomized block design which was composed of six treatments with 10 replications, those were T0 as controls ((90% CEP-2 with BSA + 10% egg yolk); T1 (83.84% CEP-2 + 6.16% cattle blood serum + 10% egg yolk); T2 (81.84% CEP-2 + 8.16% cattle blood serum + 10% egg yolk); dan T3 (90% CEP-2 + 0,4% egg white + 10% egg yolk); T4 (90% CEP-2 + 0.8% egg white + 10% egg yolk); and T5 (90% CEP-2 without BSA + 10% egg yolk). Parameters measured were the percentage of motility, viability, and abnormality of sperms. Results of research after 48 hours of storage showed that the percentage of sperm motility in T0, T1, T2, T3, T4, and T5 were 40.50±5.90, 36±36.16, 34.00±6.58, 40.50±3.69, 38.50±3.37, and 38.50±4.12, respectively, while the percentage of sperms viability were 75.16±4.30, 70.50±2.88, 73.80±2.80, 74.80±3.30, 75.13±3.13, and 74.03±4.13, respectively, and the percentage of sperms abnormality were 10.14±2.34, 10.62±1.34, 11.33±2.00, 10.94±2.82, 10.02±1.95, and 10.78±1.96, respectively. In conclusion, CEP-2 diluent with or without the addition of 19% egg yolk in BSA and the substitution of BSA with 0.4-0.8% egg white can maintain semen quality to hour of 48 in cold storage.

Retardation of some drugs in thin-layer chromatographic systems with impregnated silica gel plates with hen's egg white and bovine serum albumin

2020 ◽  
Vol 1625 ◽  
pp. 461277
Author(s):  
Kamila Jaglińska ◽  
Beata Polak ◽  
Anna Klimek-Turek ◽  
Paweł Pomastowski ◽  
Bogusław Buszewski ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Thin Layer ◽  
Silica Gel ◽  
Bovine Serum ◽  
Egg White ◽  
Hen’S Egg

Iron absorption in humans: bovine serum albumin compared with beef muscle and egg white

1988 ◽  
Vol 47 (1) ◽  
pp. 102-107 ◽  
Author(s):  
R F Hurrell ◽  
S R Lynch ◽  
T P Trinidad ◽  
S A Dassenko ◽  
J D Cook
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Iron Absorption ◽  
Egg White ◽  
Beef Muscle

191 ALLEVIATION OF THE TWO-CELL BLOCK OF ICR, ddY, AND AKR MOUSE EMBRYOS BY BOVINE SERUM ALBUMIN

2007 ◽  
Vol 19 (1) ◽  
pp. 212
Author(s):  
K. Ono ◽  
R. Ohishi ◽  
H. Imai ◽  
M. Yamada
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Culture Media ◽  
Mouse Embryos ◽  
Control Group ◽  
Cell Block ◽  
Cell Stage ◽  
Developmental Rates

Bovine serum albumin (BSA) is an embryotrophic macromolecule used in embryo culture media and improves embryo development in vitro. However, when 1-cell embryos from some strains of mouse were cultured in traditional medium, even with BSA, developmental arrest occurred at the 2-cell stage, termed '2-cell block'. The developmental block is known to be alleviated by adding EDTA to the medium for ICR and ddY strains, and deleting phosphate from the medium for the AKR strain. Recently, our preliminary experiments revealed that the 2-cell block is relieved by adding deionized BSA (d-BSA) to the medium for the ICR strain. Thus, in the present study, we investigated whether d-BSA could rescue the embryos from ICR, ddY, and AKR strains from the 2-cell block. Fertilized 1-cell embryos were collected 20 h post-hCG from superovulated ICR, ddY, and AKR females (8-week-old) that had been mated with the ICR strain of males. Stock solutions (15%) of commercially available fraction V BSA, ovalbumin (ova), and γ-globulin (γG) were deionized over a mixed-bed ion adsorption resin. Embryos were cultured in EDTA-depleted KSOM medium with or without these deionized or non-deionized proteins at 37�C under 5% CO2 in air for 4 days. Experiments were done in at least 3 replicates, and the statistical analyses of the data were done by ANOVA and Fisher's PLDS test. To observe the distribution of BSA in the embryos from the 1-cell to the blastocyst stages, immunofluorescence study was performed using anti-BSA antibody with a laser confocal microscope. The developmental rates to the 4-cell stage of 1-cell embryos cultured in medium without (control group) or with BSA at 0.3% in ICR and 0.6% in ddY and AKR (BSA group) were very low (ICR: 10% (4/38) and 37% (17/47); ddY: 9% (7/73) and 23% (9/37); AKR: 0% (0/60) and 0% (18/30), respectively). However, when embryos were cultured with d-BSA at 0.3% in ICR and 0.6% in ddY and AKR, the rates to the 4-cell stage significantly increased (ICR: 91% (51/56), ddY: 82% (61/76), AKR: 82% (50/60) vs. control group or BSA group: P < 0.05), and development to the blastocyst stage was observed (ICR: 79% (44/56), ddY: 65% (47/76), AKR: 63% (38/60)). When ICR embryos were cultured with 0.3% deionized-ova or deionized-�G, no significant increase was observed in developmental rates to the 4-cell stage (25% (10/40) and 24% (10/42), respectively). We next examined the critical culture period for the beneficial effects of d-BSA and intracellular distribution of BSA using ddY mouse embryos. It was found that exposure to d-BSA during the late 1-cell (24 h post-hCG) and early 2-cell stages (42 h post-hCG) promoted the development beyond the 2-cell stage. The distribution of BSA in the cytoplasm of embryos at any stage was observed. Interestingly, BSA localized in the nuclei of embryos during the late 1-cell and early 2-cell stages. In conclusion, our results suggest that BSA itself has a potential to remove the 2-cell block in ICR, ddY, and AKR strains. In addition, nuclear localization of BSA may play a key role in regulating the development beyond the 2-cell stage in the mouse embryos.

Protein Foaming-Consolidation Method for Fabrication of High Performance Porous Bioceramics

2012 ◽  
Vol 622-623 ◽  
pp. 1759-1763
Author(s):  
Iis Sopyan ◽  
Suryanto ◽  
Ahmad Fadli ◽  
Ramesh Singh
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Porous Materials ◽  
Bovine Serum ◽  
High Performance ◽  
Biomedical Application ◽  
Egg Yolk ◽  
Egg White ◽  
Porous Bioceramics

Protein foaming-consolidation method is a facile technique for production of porous materials using egg yolk as pore creating agent. Usage of the yolk in the process offers a number of advantages over other proteins such as egg white and bovine serum albumin (BSA). Various materials were successfully fabricated using this technique. The present paper gives a brief review of the preparation and characterization of the materials through protein foaming-consolidation technique for biomedical application.

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