Scalp Acupuncture and Treadmill Training Inhibits Neuronal Apoptosis through Activating cIAP1 in Cerebral Ischemia Rats

Stroke is the leading cause of long-term disability in developed countries. Multitudinous evidence suggests that treadmill training treatment is beneficial for balance and stroke rehabilitation; however, the need for stroke therapy remains unmet. In the present study, a cerebral ischemia rat model was established by permanent middle cerebral artery occlusion (pMCAO) to explore the therapeutic effect and mechanism of scalp acupuncture combined with treadmill training on ischemic stroke. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and neuronal nuclear protein (NeuN) double staining and cellular inhibitor of apoptosis protein-1 (cIAP1) and NeuN immunofluorescence double staining were used to detect the short-term and long-term neuroprotective effects of scalp acupuncture combined with treadmill training on pMCAO rats. In addition, the antiapoptotic effect of the combined treatment was evaluated in pMCAO rats transfected with cIAP1 shRNA. Western blotting was used to detect the relative protein expression in the caspase-8/-9/-3 activation pathway downstream of cIAP1 to further clarify its regulatory mechanism. Our results showed that scalp acupuncture combined with treadmill training successfully achieved short-term and long-term functional improvement within 14 days after stroke, significantly inhibited neuronal apoptosis, and upregulated the expression of cIAP1 protein in the ischemic penumbra area of the ischemic brain. However, no significant functional improvement and antiapoptotic effect were found in pMCAO rats transfected with cIAP1 shRNA. Western blotting results showed that the combined therapy markedly inhibited the activation of the caspase-8/-9/-3 pathway. These findings indicate that scalp acupuncture combined with treadmill training therapy may serve as a more effective alternative modality in the treatment of ischemic stroke, playing an antiapoptotic role by upregulating the expression of cIAP1 and inhibiting the activation of the caspase-8/-9/-3 pathway.

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Effects of treadmill training on the balance, functional capacity and quality of life in Parkinson’s disease: A randomized clinical trial

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2018-0245 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

Paria Arfa-Fatollahkhani ◽

Afsaneh Safar Cherati ◽

Seyed Amir Hasan Habibi ◽

Gholam Ali Shahidi ◽

Ahmad Sohrabi ◽

...

Keyword(s):

Quality Of Life ◽

Parkinson’S Disease ◽

Clinical Trial ◽

Functional Capacity ◽

Treadmill Training ◽

Moderate Intensity ◽

P Value ◽

Short Term

AbstractBackgroundThere is growing evidence that exercise modalities have considerable effects on Parkinson’s disease (PD). This trial aimed to provide a more detailed viewpoint of short-term and long-term treadmill training (TT) effects on some motor and non-motor features of PD.MethodsIn this prospective, randomized, single-blind clinical trial, 20 mild to moderate PD patients, admitted in Rasoul-e-Akram hospital in Tehran, Iran, were randomly allocated in case (11) and control (9) groups. Treadmill intervention was performed at moderate intensity with 60% of heart rate reserved (HRR) in two 30-min sessions/week for a duration of 10 weeks. Both the groups were evaluated for three times; at the baseline, 2 months later and then 2 months after the second evaluation. We assigned the Timed Up and Go test (TUG), 6-min walk test (6MW), and the SF-8 healthy questionnaire, for assessment of balance, functional capacity, and Quality of life (QoL), respectively.ResultsBalance and functional capacity were significantly improved in the case group after the intervention (TUG p-value: 0.003, 6MW p-value: 0.003). Moreover, the long-term analysis revealed significant results as well (TUG p-value: 0.001, 6MW p-value: 0.004). Mental condition’s scores of SF-8 in cases were not statistically different in short-term follow-up (F/U). However, analysis illustrated p-value: 0.016 for long-term assessment. The intervention induced significant changes in physical condition’s scores in both of the F/Us (PC p-value: 0.013).ConclusionsThis study provides evidence that a TT of mild to moderate intensity has significant and persistent benefits for the balance, functional capacity, and QoL in PD.

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QEEG Prognostic Value in Acute Stroke

Clinical EEG and Neuroscience ◽

10.1177/155005940703800312 ◽

2007 ◽

Vol 38 (3) ◽

pp. 155-160 ◽

Cited By ~ 30

Author(s):

E. Cuspineda ◽

C. Machado ◽

L. Galán ◽

E. Aubert ◽

M. A. Alvarez ◽

...

Keyword(s):

Ischemic Stroke ◽

Total Power ◽

Cerebral Stroke ◽

Short Term ◽

Term Outcome ◽

Long Term Outcome ◽

Outcome Scores ◽

Dependent Variables ◽

Short And Long Term

The objective of our study is to determine the predictive value of QEEG in patients suffering from an acute ischemic cerebral stroke. Twenty-eight patients were studied within the first 72 hours of clinical evolution of middle cerebral artery territory ischemic stroke. Thirty-seven QEEG recordings were obtained: 13 in the first 24 hours after cerebral stroke onset, 9 between 24–48 hours and 15 between 48–72 hours. Absolute Energies (AE) were the QEEG selected variables for statistical analysis: first, AE Z values were calculated using the Cuban QEEG norms, then the maximum and minimum AE Z values were selected within each frequency band and total power. The medians of the five neighboring Z values were also chosen. Regression models were estimated using the RANKIN scores as dependent variables and the selected QEEG variables as independent, then outcome predictions at hospital discharge and 3 months later were calculated. Percentages of concordance and errors between the estimated and real outcome scores were obtained. Alpha and theta AE were the best predictor for short-term outcome and delta AE for long-term outcome. We conclude that QEEG performed within the first 72 hours of ischemic stroke might be a powerful tool predicting short-and long-term outcome.

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Treadmill training improves neurological deficits and suppresses neuronal apoptosis in cerebral ischemic stroke rats

Neural Regeneration Research ◽

10.4103/1673-5374.253523 ◽

2019 ◽

Vol 14 (8) ◽

pp. 1387 ◽

Cited By ~ 1

Author(s):

Xu Chen ◽

Li-Mei Cao ◽

Zhi-Qiang Dong ◽

Qiang Li

Keyword(s):

Ischemic Stroke ◽

Neuronal Apoptosis ◽

Treadmill Training ◽

Neurological Deficits ◽

Cerebral Ischemic Stroke ◽

Cerebral Ischemic

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Elevated Calcium after Acute Ischemic Stroke: Association with a Poor Short-Term Outcome and Long-Term Mortality

Journal of Stroke ◽

10.5853/jos.2015.17.1.54 ◽

2015 ◽

Vol 17 (1) ◽

pp. 54 ◽

Cited By ~ 14

Author(s):

Jong-Won Chung ◽

Wi-Sun Ryu ◽

Beom Joon Kim ◽

Byung-Woo Yoon

Keyword(s):

Ischemic Stroke ◽

Acute Ischemic Stroke ◽

Short Term ◽

Term Outcome ◽

Short Term Outcome ◽

Long Term Mortality

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NCMP-05. THE INCIDENCE AND IMPACT OF POST-OPERATIVE STROKE IN SURGERY FOR LGG

Neuro-Oncology ◽

10.1093/neuonc/noz175.751 ◽

2019 ◽

Vol 21 (Supplement_6) ◽

pp. vi180-vi180

Author(s):

Asaf Berger ◽

Garry Tzarfati ◽

Mathias Costa ◽

Marga Serafimova ◽

Akiva Korn ◽

...

Keyword(s):

Ischemic Stroke ◽

Performance Status ◽

Tumor Resection ◽

Neurological Deficits ◽

Functional Improvement ◽

Motor Deficit ◽

Low Grade ◽

Post Surgery ◽

One Year

Abstract BACKGROUND Postoperative neurological deficits may outweigh the benefit conferred by maximal resection of gliomas. We evaluated the incidence of ischemic events in patients undergoing surgery for low-grade gliomas (LGG) and the long-term neurological and cognitive sequelae. METHODS Between 2013–2017, 168 patients underwent surgical resection or biopsy for LGG at our center. A full dataset, including pre- and postoperative magnetic resonance imaging (MRI) and long-term clinical evaluation findings, was available for 82 patients (study group). Ischemic complications, overall and progression-free survival, and functional and neurocognitive outcomes were evaluated. RESULTS The immediate postoperative MRI revealed an acute ischemic stroke adjacent to the tumor resection cavity in 19 patients (23%), 13 of whom developed new neurological deficits due to the ischemic event. Infarcts were more common in patients with recurrent tumors, especially those involving the Sylvian fissure (p< 0.05). Surgery for insular gliomas had the strongest association with postoperative infarcts. Survival of patients w/wo a postoperative infarct was the same. The median Karnofsky-Performance Status was lower for the infarct group vs. the non-infarct group at 3 months post-surgery (p=0.016), with a gradual significant improvement for the former over one year (p=0.04). Immediately after surgery, 27% of the patients without infarcts and 58% of those with infarcts experienced a new motor deficit (p=0.037), decreasing to 16% (p=0.028) and 37% (p=0.001), respectively, at one year. Neurocognitive analysis findings before and 3 months after surgery were unchanged, but patients with an infarct had a significant decrease in naming (p=0.04). Confusion during awake craniotomy was a strong predictor of an ischemic stroke. CONCLUSIONS Intraoperative strokes are more prevalent among patients who undergo recurrent surgeries, especially in the insula. Although they do not affect survival, these strokes negatively impact the patients’ activity and performance status, especially during the first 3 postoperative months, with gradual functional improvement over one year.

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Transient focal cerebral ischemia induces long-term cognitive function deficit in an experimental ischemic stroke model

Neurobiology of Disease ◽

10.1016/j.nbd.2013.06.014 ◽

2013 ◽

Vol 59 ◽

pp. 18-25 ◽

Cited By ~ 57

Author(s):

Wenjun Li ◽

Renqi Huang ◽

Ritu A. Shetty ◽

Nopporn Thangthaeng ◽

Ran Liu ◽

...

Keyword(s):

Ischemic Stroke ◽

Cognitive Function ◽

Cerebral Ischemia ◽

Focal Cerebral Ischemia ◽

Stroke Model ◽

Transient Focal Cerebral Ischemia

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Exosomes from LPS-stimulated macrophages induce neuroprotection and functional improvement after ischemic stroke by modulating microglial polarization

Biomaterials Science ◽

10.1039/c8bm01449c ◽

2019 ◽

Vol 7 (5) ◽

pp. 2037-2049 ◽

Cited By ~ 30

Author(s):

Yan Zheng ◽

Ruyi He ◽

Peng Wang ◽

Yijie Shi ◽

Liang Zhao ◽

...

Keyword(s):

Ischemic Stroke ◽

Cerebral Ischemia ◽

Ischemia Reperfusion ◽

Functional Improvement ◽

Ischemic Insult ◽

Microglial Polarization ◽

Cerebral Ischemia Reperfusion

Inflammation occurs throughout the progression of cerebral ischemia/reperfusion and mediates myriads of pathological events following an ischemic insult.

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Chronic Testosterone Deprivation Sensitizes the Middle-Aged Rat Brain to Damaging Effects of Testosterone Replacement

Neuroendocrinology ◽

10.1159/000504445 ◽

2019 ◽

Vol 110 (11-12) ◽

pp. 914-928 ◽

Cited By ~ 1

Author(s):

Charity Smith ◽

Jo Contreras-Garza ◽

Rebecca L. Cunningham ◽

Jessica M. Wong ◽

Philip H. Vann ◽

...

Keyword(s):

Oxidative Stress ◽

Cerebral Ischemia ◽

Focal Cerebral Ischemia ◽

Male Rats ◽

Testosterone Replacement ◽

Middle Aged ◽

Short Term ◽

Stress Levels ◽

Middle Aged Male

Introduction: An increasing number of middle-aged men are being screened for low testosterone levels and the number of prescriptions for various forms of testosterone replacement therapy (TRT) has increased dramatically over the last 10 years. However, the safety of TRT has come into question with some studies suggesting increased morbidity and mortality. Objective: Because the benefits of estrogen replacement in postmenopausal women and ovariectomized rodents are lost if there is an extended delay between estrogen loss and replacement, we hypothesized that TRT may also be sensitive to delayed replacement. Methods: We compared the effects of testosterone replacement after short-term (2 weeks) and long-term testosterone deprivation (LTTD; 10 weeks) in middle-aged male rats on cerebral ischemia, oxidative stress, and cognitive function. We hypothesized that LTTD would increase oxidative stress levels and abrogate the beneficial effects of TRT. Results: Hypogonadism itself and TRT after short-term castration did not affect stroke outcome compared to intact rats. However, after long-term hypogonadism in middle-aged male Fischer 344 rats, TRT exacerbated the detrimental behavioral effects of experimental focal cerebral ischemia, whereas this detrimental effect was prevented by administration of the free-radical scavenger tempol, suggesting that TRT exacerbates oxidative stress. In contrast, TRT improved cognitive performance in non-stroked rats regardless of the length of hypogonadism. In the Morris water maze, peripheral oxidative stress was highly associated with decreased cognitive ability. Conclusions: Taken together, these data suggest that TRT after long-term hypogonadism can exacerbate functional recovery after focal cerebral ischemia, but in the absence of injury can enhance cognition. Both of these effects are modulated by oxidative stress levels.

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Short-Term Antifibrinolytic Therapy Before Early Aneurysm Treatment in Subarachnoid Hemorrhage: Effects on Rehemorrhage, Cerebral Ischemia, and Hydrocephalus

Neurosurgery ◽

10.1227/neu.0b013e3181ebaa36 ◽

2010 ◽

Vol 67 (4) ◽

pp. 935-940 ◽

Cited By ~ 46

Author(s):

Mark R Harrigan ◽

Kiran F Rajneesh ◽

Agnieszka A Ardelt ◽

Winfield S Fisher

Keyword(s):

Ischemic Stroke ◽

Subarachnoid Hemorrhage ◽

Cerebral Ischemia ◽

Aneurysmal Subarachnoid Hemorrhage ◽

Aminocaproic Acid ◽

Neurological Deficits ◽

Short Term ◽

Symptomatic Vasospasm ◽

Term Administration ◽

Ischemic Complications

Abstract BACKGROUND: Long-term administration of the antifibrinolytic agent epsilon aminocaproic acid (EACA) reduces the rate of rehemorrhage in patients with aneurysmal subarachnoid hemorrhage (SAH), but is associated with cerebral ischemia. OBJECTIVE: To evaluate short-term administration of EACA before early surgery in patients with SAH. METHODS: Retrospective review of 356 patients admitted between June 2002 and December 2007 with a diagnosis of aneurysmal SAH. Medical records were reviewed to determine SAH risk factors, clinical grade at the time of admission, and incidence of rehemorrhage, permanent new-onset focal neurological deficits, computed tomography evidence of cerebral infarction, symptomatic vasospasm, and hydrocephalus. RESULTS: Patients underwent treatment of the ruptured aneurysm an average of 47.4 hours after admission and received an average total dose of 40.6 g of EACA. The mean length of time of administration of EACA was 35.6 hours. There was a total of 5 rehemorrhages, for an overall rebleeding rate of 1.4% and a rate of rehemorrhage per 24-hour period of 0.71%. Overall, the rates of symptomatic vasospasm and permanent neurological deficits attributable to ischemic stroke were 11.5% and 7.2%, respectively, and the incidence of shunt-dependent hydrocephalus was 42.3%. Patients who were treated with coiling had higher rates of symptomatic vasospasm and ischemic complications than patients who had surgery. CONCLUSION: Short-term administration of EACA is associated with rates of rehemorrhage, ischemic stroke, and symptomatic vasospasm that compare favorably with historical controls. The rate of hydrocephalus is relatively high and may be attributable to EACA treatment.

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