scholarly journals The Bridge between Screening and Assessment: Establishment and Application of Online Screening Platform for Food Risk Substances

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Kang Hu ◽  
Shaoming Jin ◽  
Hong Ding ◽  
Jin Cao
Keyword(s):  
Food Safety ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
The Internet ◽  
Screening Model ◽  
Standard Spectrum ◽  
Screening Platform ◽  
And Control ◽  
Resolution Mass ◽  
Standard Library

In order to improve the risk identification ability of the technical support system of food safety supervision, an online screening platform for food risk substances (hereafter referred to as “platform”) was established. The platform aims at the qualitative analysis of unknown compounds and consists of three parts: a standard spectrum library, screening model, and online comparison module. The standard library contains the standard spectra of 527 food risk substances by high-performance liquid chromatography/high-resolution mass spectrometry. The screening comparison algorithm, the core of the screening model, is obtained through the improvement of the existing spectral library search algorithm. The inspector uploads the original spectrum file through the online comparison module; the online comparison module calls the corresponding script to convert the original spectrum file into a standard spectrum file and then uses the screening and comparison algorithm to achieve online real-time comparison. The comparison results are used to determine whether the sample to be tested contains the food risk substances contained in the standard library, so as to realize the preliminary screening of potential food risk substances. The platform supports the spectrogram data format of mainstream instrument manufacturers. The standard spectrogram database can be coconstructed and shared by cooperative laboratories to effectively enrich the types of food risk substances. Through laboratory comparison, data calibration, and model optimization, the screening accuracy of the platform can reach more than 97%. The platform adopts the Internet online screening method, which greatly facilitates the risk investigation and control of national food safety inspection and testing institutions. At the same time, the construction of the screening platform for food risk substances based on high-performance liquid chromatography/high-resolution mass spectrometry, the Internet, big data, and other technologies will provide a new technical means for food safety risk management and control. Hence, it can build a bridge between the screening of risk substances and illegally added substances, as well as risk assessment, risk management, and control.

scholarly journals Ultra-High Performance Liquid Chromatography-High Resolution Mass Spectrometry and High-Sensitivity Gas Chromatography-Mass Spectrometry Screening of Classic Drugs and New Psychoactive Substances and Metabolites in Urine of Consumers

2021 ◽  
Vol 22 (8) ◽  
pp. 4000
Author(s):  
Emilia Marchei ◽  
Maria Alias Ferri ◽  
Marta Torrens ◽  
Magí Farré ◽  
Roberta Pacifici ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Sensitivity ◽  
Gas Chromatography Mass Spectrometry ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Resolution Mass

The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2.1 mm, 2.6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services.

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