Mass Spectrometry and Computer Simulation Predict the Interactions of AGPS and HNRNPK in Glioma

Ether lipids are overexpressed in malignant tumor and play an important role in tumor process. Glioma is the most common malignant central nervous system tumor, and the content of ether lipids is higher than that of normal tissues. Alkylglycerone phosphate synthase (AGPS) is a key enzyme in the synthesis of ether esters and plays a vital role in maintaining the morphology and pathogenic properties of tumor cells. The cell proliferation and the content of tumor-related lipid such as monoalkylglycerol ether (MAGe), lysophosphatidic acid ether (LPAe), lysophosphatidylcholine ether (LPCe), lysophosphatidylethanolamine ether (LPEe), phosphatidyl inositol (PI), phosphatidylcholine (PC), and phosphatidylserine (PS) were suppressed after AGPS silencing in U251, H4, and TJ905 cells; however, heterogeneous nuclear ribonucleoprotein K (HNRNPK) could reverse the above phenomenon such as cellar proliferation and ether lipid secretion. We found that HNRNPK was the target protein of AGPS by coimmunoprecipitation and mass spectrometry assay and verified by western blot assay in U251 cells. It confirmed that AGPS and HNRNPK are coexpressed in the cellular nucleus by a confocal laser microscope. The main protein-protein interaction mechanism between AGPS and HNRNPK is hydrogen bond, conjugation bond, hydrophobic bond, and electrostatic force by computer simulation prediction.

Download Full-text

Structure of an n-Heptane/Toluene Flame: Molecular Beam Mass Spectrometry and Computer Simulation Investigations

Физика горения и взрыва ◽

10.15372/fgv20160203 ◽

2016 ◽

Keyword(s):

Mass Spectrometry ◽

Computer Simulation ◽

Molecular Beam ◽

Molecular Beam Mass Spectrometry

Download Full-text

Ligand size distribution of phenanthroline-functionalized polyethylene glycol-iron(II) complexes determined by electrospray ionization mass spectrometry and computer simulation

Journal of the American Society for Mass Spectrometry ◽

10.1016/j.jasms.2010.04.019 ◽

2010 ◽

Vol 21 (9) ◽

pp. 1561-1564

Author(s):

Ákos Kuki ◽

Miklós Nagy ◽

Lajos Nagy ◽

Miklós Zsuga ◽

Sándor Kéki

Keyword(s):

Mass Spectrometry ◽

Computer Simulation ◽

Polyethylene Glycol ◽

Electrospray Ionization ◽

Size Distribution ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass

Download Full-text

Micro-System Displacement and Profile Measurement By an Integrated Photon Tunneling and Confocal Microscope

Journal of Mechanics ◽

10.1017/s1727719100004081 ◽

2002 ◽

Vol 18 (4) ◽

pp. 173-183

Author(s):

Wen-Jong Chen ◽

Chih-Kung Lee ◽

Shui-Shong Lu ◽

Long-Sun Huang ◽

Ta-Shun Chu ◽

...

Keyword(s):

Laser Scanning ◽

Electrostatic Force ◽

Three Dimensional ◽

Confocal Laser Scanning Microscope ◽

Confocal Laser ◽

Profile Measurement ◽

Light Sources ◽

Halogen Lamp ◽

Photon Tunneling ◽

Micro Systems

ABSTRACTAn integrated optical method for measuring deformation of micro-mechanical systems with better than sub-micron resolutions is detailed. Both a confocal laser scanning microscope and a photon tunneling microscope were integrated into a single microscopy system due to their complimentary capabilities for examining sub-micrometer deformations. A halogen lamp and laser were adopted as the two light sources for the measurements. Since topographic information of samples up to a 15μm by 15μm area can be measured, a three-dimensional displacement field of the sample was extracted by comparing topographies of the same specimen area before and after deformation. The bending and twisting deformation of a micro-mirror driven by the electrostatic force was measured to demonstrate the capability of this newly developed instrument. The experimental data obtained agrees reasonably well with the theoretical results calculated by adopting an analytical solution and a finite element method. The small discrepancy in the result can be traced to the surface roughness effect, which is often non-negligible in micro-systems.

Download Full-text

1-O-Alkylglycerol Ether Lipids in Two Holothurian Species: Cucumaria japonica and C. okhotensis

Natural Product Communications ◽

10.1177/1934578x0700200913 ◽

2007 ◽

Vol 2 (9) ◽

pp. 1934578X0700200

Author(s):

Viatcheslav Rybin ◽

Konstantin Pavel ◽

Dmitry Mitrofanov

Keyword(s):

Ether Lipid ◽

Ether Lipids ◽

Alkyl Radicals ◽

Hydrocarbon Chains ◽

Species Specific ◽

Cucumaria Japonica

1- O-Alkylglycerol ether lipids were investigated in two holothurian species, Cucumaria japonica and C. okhotensis. Species specific differences in the content of 1- O-alkylglycerol ether lipids were found. Some alkyl radicals of the 1- O-alkylglycerol ether lipid molecules contained branched hydrocarbon chains. The ratio between the content of linear and branched saturated 1- O-alkylglycerol ether lipids was 1.53 in C. okhotensis and 0.55 in C. japonica.

Download Full-text

Analysis of archaeal core ether lipids using Time of Flight?Secondary Ion Mass Spectrometry (ToF-SIMS): Exploring a new prospect for the study of biomarkers in geobiology

Geobiology ◽

10.1111/j.1472-4669.2006.00093.x ◽

2006 ◽

Vol 0 (0) ◽

pp. 061221060249003-??? ◽

Cited By ~ 2

Author(s):

V. THIEL ◽

J. TOPORSKI ◽

G. SCHUMANN ◽

P. SJÖVALL ◽

J. LAUSMAA

Keyword(s):

Mass Spectrometry ◽

Secondary Ion Mass Spectrometry ◽

Time Of Flight ◽

Ether Lipids ◽

Tof Sims ◽

Ion Mass Spectrometry ◽

Secondary Ion

Download Full-text

Low temperature diamond growth using CO2/CH4 plasmas: Molecular beam mass spectrometry and computer simulation investigations

Journal of Applied Physics ◽

10.1063/1.1333031 ◽

2001 ◽

Vol 89 (2) ◽

pp. 1484-1492 ◽

Cited By ~ 47

Author(s):

James R. Petherbridge ◽

Paul W. May ◽

Sean R. J. Pearce ◽

Keith N. Rosser ◽

Michael N. R. Ashfold

Keyword(s):

Mass Spectrometry ◽

Computer Simulation ◽

Low Temperature ◽

Molecular Beam ◽

Diamond Growth ◽

Molecular Beam Mass Spectrometry

Download Full-text

Serum Biomarker Identification by Mass Spectrometry in Acute Aortic Dissection

Cellular Physiology and Biochemistry ◽

10.1159/000485954 ◽

2017 ◽

Vol 44 (6) ◽

pp. 2147-2157 ◽

Cited By ~ 6

Author(s):

Yong Ren ◽

Qizhu Tang ◽

Wenwei Liu ◽

Yongqian Tang ◽

Rui Zhu ◽

...

Keyword(s):

Mass Spectrometry ◽

Aortic Dissection ◽

Peripheral Blood ◽

Metabolic Pathways ◽

Healthy Subjects ◽

Ether Lipid ◽

Intramural Hematoma ◽

Differentially Expressed ◽

Metabolome Analysis ◽

Hypertensive Patients

Background/Aims: Aortic dissection (AD) is also known as intramural hematoma. This study aimed to screen peripheral blood biomarkers of small molecule metabolites for AD using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Methods: Sera from 25 healthy subjects, 25 patients with well-established AD, and 25 patients with well-established hypertension were investigated by HPLC-MS to detect metabolites, screen differentially expressed metabolites, and analyze metabolic pathways. Results: Twenty-six and four metabolites were significantly up- and down-regulated in the hypertensive patients compared with the healthy subjects; 165 metabolites were significantly up-regulated and 109 significantly down-regulated in the AD patients compared with the hypertensive patients. Of these metabolites, 35 were up-regulated and 105 down-regulated only in AD patients. The metabolites that were differentially expressed in AD are mainly involved in tryptophan, histidine, glycerophospholipid, ether lipid, and choline metabolic pathways. As AD alters the peripheral blood metabolome, analysis of peripheral blood metabolites can be used in auxiliary diagnosis of AD. Conclusion: Eight metabolites are potential biomarkers for AD, 3 of which were differentially expressed and can be used for auxiliary diagnosis of AD and evaluation of treatment effectiveness.

Download Full-text

Ether Lipid Deficiency in Mice Produces a Complex Behavioral Phenotype Mimicking Aspects of Human Psychiatric Disorders

International Journal of Molecular Sciences ◽

10.3390/ijms20163929 ◽

2019 ◽

Vol 20 (16) ◽

pp. 3929 ◽

Cited By ~ 10

Author(s):

Fabian Dorninger ◽

Anna Gundacker ◽

Gerhard Zeitler ◽

Daniela D. Pollak ◽

Johannes Berger

Keyword(s):

Psychiatric Disorders ◽

Treatment Strategies ◽

Ether Lipid ◽

Detailed Examination ◽

Behavioral Phenotype ◽

Emotional Behavior ◽

Mental Illnesses ◽

Ether Lipids ◽

Behavioral Abnormalities ◽

Deficient Mice

Ether lipids form a specialized subgroup of phospholipids that requires peroxisomes to be synthesized. We have previously detected that deficiency in these lipids leads to a severe disturbance of neurotransmitter homeostasis and release as well as behavioral abnormalities, such as hyperactivity, in a mouse model. Here, we focused on a more detailed examination of the behavioral phenotype of ether lipid-deficient mice (Gnpat KO) and describe a set of features related to human psychiatric disorders. Gnpat KO mice show strongly impaired social interaction as well as nestlet shredding and marble burying, indicating disturbed execution of inborn behavioral patterns. Also, compromised contextual and cued fear conditioning in these animals suggests a considerable memory deficit, thus potentially forming a connection to the previously determined ether lipid deficit in human patients with Alzheimer’s disease. Nesting behavior and the preference for social novelty proved normal in ether lipid-deficient mice. In addition, we detected task-specific alterations in paradigms assessing depression- and anxiety-related behavior. The reported behavioral changes may be used as easy readout for the success of novel treatment strategies against ether lipid deficiency in ameliorating nervous system-associated symptoms. Furthermore, our findings underline that ether lipids are paramount for brain function and demonstrate their relevance for cognitive, social, and emotional behavior. We hereby substantially extend previous observations suggesting a link between deficiency in ether lipids and human mental illnesses, particularly autism and attention-deficit hyperactivity disorder.

Download Full-text

Addition of lipid substituents of mammalian protein glycosylphosphoinositol anchors

Molecular and Cellular Biology ◽

10.1128/mcb.14.1.21-31.1994 ◽

1994 ◽

Vol 14 (1) ◽

pp. 21-31

Author(s):

N Singh ◽

R A Zoeller ◽

M L Tykocinski ◽

P B Lazarow ◽

A M Tartakoff

Keyword(s):

Lipid Synthesis ◽

Ether Lipid ◽

Raw 264.7 ◽

Placental Alkaline Phosphatase ◽

Ether Lipids ◽

Wild Type ◽

Animal Cells ◽

Express Cell ◽

Mutant Cells ◽

Mammalian Protein

A single metabolic path leading to synthesis of ether lipids is known in animal cells, the major products of which are plasmalogens. To learn whether this peroxisomal path is also responsible for the synthesis of base-resistant lipid components of glycosylphosphoinositol (GPI)-anchored membrane proteins, we have investigated the structure of anchor precursor mannolipids both in wild-type cells (CHO-K1 and a macrophage-like line, RAW 264.7) and in two corresponding mutant cells in which ether lipid biosynthesis is severely impaired. We observe that the precursor mannolipids of both the wild-type and mutant cells do not include alkylglycerol. Nevertheless, both wild-type and mutant cells express cell surface GPI-anchored placental alkaline phosphatase (AP) which includes alkali-resistant hydrophobic chains in its anchor moiety. Thus, (i) in normal AP GPI anchor synthesis, any ether-linked substituents must be added either immediately before, during, or after anchor addition to AP, and (ii) the classical peroxisomal path for ether lipid synthesis appears not to contribute to the synthesis of GPI anchors.

Download Full-text