scholarly journals Protective Effects of Total Flavonoids from Lysimachia christinae on Calcium Oxalate-Induced Oxidative Stress in a Renal Cell Line and Renal Tissue

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Jian Wang ◽  
Jia-Jian Chen ◽  
Jia-Hao Huang ◽  
Bo-Dong Lv ◽  
Xiao-Jun Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Calcium Oxalate ◽  
Cell Line ◽  
Renal Cell ◽  
Renal Tissue ◽  
Heme Oxygenase 1 ◽  
Crystal Formation ◽  
Total Flavonoids ◽  
Quinone Oxidoreductase ◽  
Renal Cell Line

Oxidative stress (OS) in renal tubular epithelial cells (RTECs) is induced by calcium oxalate (CaOx) stones and plays an important role in the pathology of CaOx nephrolithiasis. The nuclear factor-E2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway is an important endogenous antioxidant pathway. Flavonoids are compounds with 2-phenylchromone as the basic mother nucleus and are natural antioxidant components of Lysimachia christinae. Our previous studies demonstrated that the total flavonoids from L. christinae (TFL) reduced calcium and oxalic acid concentrations in urine, thus inhibiting CaOx stone formation. We also showed that TFL can reduce OS in renal tissue. However, whether TFL inhibit the formation of CaOx stones through the Nrf2/ARE pathway requires further investigation. Here, we found that TFL protected against injury to a renal cell line and renal tissue, reduced CaOx-induced OS in renal tissue, and reduced CaOx crystal formation. In addition, TFL significantly increased nuclear Nrf2 and the expression of the downstream antioxidant genes heme oxygenase 1 (HO-1) and NAD(P)H quinone oxidoreductase 1 (NQO-1). Furthermore, TFL increased superoxide dismutase (SOD) activity and decreased the malondialdehyde (MDA) content, thereby alleviating OS in RTECs. Silencing Nrf2 expression blocked the protective effect of TFL on CaOx-induced OS. Taken together, our findings indicate that TFL reduce CaOx-induced OS in renal tissue by activating the Nrf2/ARE pathway.

scholarly journals An in vitroassessment of panel of engineered nanomaterials using a human renal cell line: cytotoxicity, pro-inflammatory response, oxidative stress and genotoxicity

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Ali Kermanizadeh ◽  
Sandra Vranic ◽  
Sonja Boland ◽  
Kevin Moreau ◽  
Armelle Baeza-Squiban ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
Cell Line ◽  
Renal Cell ◽  
Engineered Nanomaterials ◽  
Renal Cell Line

scholarly journals Protective Effects of Total Flavonoids from Lysimachia Christinae on Calcium Oxalate-Induced Oxidative Stress in a Renal Cell Line and Renal Tissue

2020 ◽  
Author(s):  
Jian Wang ◽  
Jiajian Chen ◽  
Jiahao Huang ◽  
Bodong Lv ◽  
Xiaojun Huang ◽  
...  
Keyword(s):  
Stone Formation ◽  
Antioxidant Response ◽  
Renal Tissue ◽  
Crystal Formation ◽  
Related Factor ◽  
Total Flavonoids ◽  
Nuclear Factor ◽  
Antioxidant Response Element ◽  
Caox Crystal ◽  
Renal Cell Line

Abstract Background: Flavonoids are compounds with 2-phenylchromone as the basic mother nucleus and are natural antioxidant components of Lysimachia christinae. We previously demonstrated that total flavonoids from L. christinae (TFL) reduce calcium and oxalic acid concentrations in urine and can reduce oxidative stress (OS) in renal tissue, thus, inhibiting calcium oxalate (CaOx) stone formation. The aim of this study was to investigate whether TFL reduced OS in renal tissue, thereby inhibiting CaOx stone formation through the nuclear factor-E2 related factor 2 (Nrf2)/antioxidant response element (ARE) pathway.Methods: The rat model of CaOx stone was established by providing rats with drinking water containing 0.5% glycol and 2% ammonium chloride. After 4 weeks of treatment with different doses of TFL (62.5, 125, and 250 mg/kg/d), body and kidney weights of the rats were measured. CaOx crystal formation was observed under the microscope and the renal tissue contents of superoxide dismutase (SOD) and malondialdehyde (MDA) were analyzed. HK-2 cells were divided into two groups: treatment with CaOx crystals or CaOx crystals + TFL. Other HK-2 cells were treated with small interfering RNA targeting nuclear factor-E2 related factor 2 (Nrf2) and divided into the same two groups. The activities of SOD and content of MDA were measured. The expression of Nrf2, heme oxygenase (HO-1), and NAD(P)H quinone oxidoreductase 1 (NQO-1) were detected using western blot.Results: In the in vitro study, TFL significantly increased nuclear Nrf2 and expression of the downstream antioxidant genes, HO-1 and NQO-1. Furthermore, TFL increased superoxide dismutase activity and decreased the malondialdehyde content, thereby alleviating OS in renal tubular epithelial cells. Moreover, silencing the expression of Nrf2 blocked the protective effect of TFL on CaOx-induced OS. In the in vivo study TFL protected the renal cell line and renal tissue against injury, reduced CaOx-induced OS in renal tissue, and reduced CaOx crystal formation.Conclusions: TFL reduces CaOx-induced OS in renal tissue by activating the nuclear Nrf2/antioxidant response element (ARE) pathway.

Testosterone increases expression of the alpha-subunit of the epithelial sodium channel (alpha-ENAC) in the human renal cell line HKC-8

2005 ◽  
Vol 113 (S 1) ◽  
Author(s):  
M Quinkler ◽  
IJ Bujalska ◽  
K Kaur ◽  
CU Onyimba ◽  
SV Hughes ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Cell Line ◽  
Renal Cell ◽  
Epithelial Sodium Channel ◽  
Alpha Subunit ◽  
Renal Cell Line

A polyherbal formulation attenuates hyperoxaluria-induced oxidative stress and prevents subsequent deposition of calcium oxalate crystals and renal cell injury in rat kidneys

2014 ◽  
Vol 11 (5) ◽  
pp. 466-471
Author(s):  
S. Bodakhe Kiran ◽  
P Namdeo Kamta ◽  
C.Patra Kartik ◽  
Machwal Lalit ◽  
K. Pareta Surendra
Keyword(s):  
Oxidative Stress ◽  
Calcium Oxalate ◽  
Renal Cell ◽  
Cell Injury ◽  
Calcium Oxalate Crystals ◽  
Polyherbal Formulation ◽  
Oxalate Crystals ◽  
Rat Kidneys

scholarly journals Resveratrol Protects C6 Astrocyte Cell Line against Hydrogen Peroxide-Induced Oxidative Stress through Heme Oxygenase 1

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64372 ◽  
Author(s):  
André Quincozes-Santos ◽  
Larissa Daniele Bobermin ◽  
Alexandra Latini ◽  
Moacir Wajner ◽  
Diogo Onofre Souza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Line ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1

scholarly journals Transport systems for polyamines in the established renal cell line LLC-PK1. Polarized expression of an Na+-dependent transporter

1990 ◽  
Vol 265 (2) ◽  
pp. 609-612 ◽  
Author(s):  
L Van Den Bosch ◽  
H De Smedt ◽  
L Missiaen ◽  
J B Parys ◽  
R Borghgraef
Keyword(s):  
Cell Line ◽  
Renal Cell ◽  
The Other ◽  
Transport Systems ◽  
Present Evidence ◽  
Cell Monolayers ◽  
Other Hand ◽  
Basolateral Side ◽  
Renal Cell Line ◽  
Polyamine Uptake

We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. Both transporters could be discriminated by their sensitivity to inhibitors, particularly rho-chloromercuriphenyl sulphate and various polycationic molecules. By using cell monolayers grown on a permeable filter support, we have found that the Na(+)-dependent polyamine uptake occurred preferentially from the basolateral side. The Na(+)-independent uptake, on the other hand, occurred to the same extent from either the apical or the basolateral side.

scholarly journals Cinnamaldehyde Ameliorates Vascular Dysfunction in Diabetic Mice by Activating Nrf2

2020 ◽  
Vol 33 (7) ◽  
pp. 610-619 ◽  
Author(s):  
Peijian Wang ◽  
Yi Yang ◽  
Dan Wang ◽  
Qiyuan Yang ◽  
Jindong Wan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Vascular Dysfunction ◽  
Mesenteric Arteries ◽  
Heme Oxygenase 1 ◽  
No Production ◽  
Related Factor ◽  
Diabetic Mice ◽  
Quinone Oxidoreductase ◽  
Nrf2 Signaling Pathway

Abstract BACKGROUND Oxidative stress is known to be associated with the development of diabetes. Cinnamaldehyde (CA) is a spice compound in cinnamon that enhances the antioxidant defense against reactive oxygen species (ROS) by activating nuclear factor erythroid-related factor 2 (Nrf2), which has been shown to have a cardioprotection effect. However, the relationship between CA and Nrf2 in diabetic vascular complications remains unclear. METHODS Leptin receptor-deficient (db/db) mice were fed normal chow or diet containing 0.02% CA for 12 weeks. The vascular tone, blood pressure, superoxide level, nitric oxide (NO) production, renal morphology, and function were measured in each group. RESULTS CA remarkably inhibited ROS generation, preserved NO production, increased phosphorylated endothelial nitric oxide synthase (p-eNOS), attenuated the upregulation of nitrotyrosine, P22 and P47 in aortas of db/db mice, and apparently ameliorated the elevation of type IV collagen, TGF-β1, P22, and P47 in kidney of db/db mice. Feeding with CA improved endothelium-dependent relaxation of aortas and mesenteric arteries, and alleviated the remodeling of mesenteric arteries in db/db mice. Additionally, dietary CA ameliorated glomerular fibrosis and renal dysfunction in diabetic mice. Nrf2 and its targeted genes heme oxygenase-1 (HO-1) and quinone oxidoreductase-1 (NQO-1) were slightly increased in db/db mice and further upregulated by CA. However, these protective effects of CA were reversed in Nrf2 downregulation mice. CONCLUSIONS A prolonged diet of CA protects against diabetic vascular dysfunction by inhibiting oxidative stress through activating of Nrf2 signaling pathway in db/db mice.

Phenothiazine-mediated depolarization of the plasma membrane in a renal cell line

1988 ◽  
Vol 37 (19) ◽  
pp. 3785-3787 ◽  
Author(s):  
James A. Scott ◽  
Alan J. Fischman ◽  
Ban-An Khaw ◽  
Carlos A. Rabito
Keyword(s):  
Plasma Membrane ◽  
Cell Line ◽  
Renal Cell ◽  
Renal Cell Line
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