scholarly journals Prevalence and Subtype Analysis of Blastocystis hominis Isolated from Patients in the Northeast of Iran

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Mitra Salehi ◽  
Jalal Mardaneh ◽  
Hamid Reza Niazkar ◽  
Mohammadhaasan Minooeianhaghighi ◽  
Elahe Arshad ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Intestinal Parasite ◽  
Gastrointestinal Symptoms ◽  
Blastocystis Hominis ◽  
Wet Mount ◽  
Meaningful Relationship ◽  
Blastocystis Spp ◽  
Pcr Products ◽  
Stool Samples ◽  
Low Prevalence

Blastocystis hominis is the most common intestinal parasite found in humans and many other hosts. Pathogenicity of Blastocystis spp. remains controversial, and it has been suggested that it may be associated with specific subtypes of the organism. This study identified the B. hominis subtypes and their prevalence rates in the northeast of Iran. A total of 1878 samples were collected from the northeast of Iran from January to December 2017. The patients’ demographic details were recorded. Samples were examined by a wet mount, and genomic DNA was extracted from positive samples. Also, PCR was done on the positive samples, and sequencing and phylogenetic analysis were subsequently performed. From 1878 collected stool samples, 152 (8.1%) Blastocystis samples were detected by the microscopic method. Of the 152 samples, Blastocystis spp. were found in 53.6% of the men and 28.9% of the women who showed clinical gastrointestinal symptoms, and a significant relationship was observed between gender and clinical symptoms ( P = 0.002 ). A meaningful relationship was found between the season and infection with this parasite ( P value = 0.003). The results of the sequencing of 22 PCR products showed the dominance of ST3, which was isolated from 10 (45.45%) patients, while ST1, ST2, and ST7 were found in 4 (18.19%), 7 (31.81%), and 1 (4.55%) patients, respectively. In this study, ST7 had a low prevalence in the northeast of Iran, and similar to previous studies, ST3 was the dominant subtype.

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results : during the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides ( 6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). Intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals Surveillance of enteropathogenic bacteria, protozoa and helminths in travellers returning from the tropics

2020 ◽  
Vol 10 (3) ◽  
pp. 147-155 ◽  
Author(s):  
Dorothea Wiemer ◽  
Norbert Georg Schwarz ◽  
Gerd-Dieter Burchard ◽  
Hagen Frickmann ◽  
Ulrike Loderstaedt ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Clinical Symptoms ◽  
Gastrointestinal Symptoms ◽  
Giardia Duodenalis ◽  
Blastocystis Hominis ◽  
Entamoeba Dispar ◽  
Pathogenic Potential ◽  
Tropical Regions ◽  
Molecular Approaches ◽  
The Tropics

AbstractDiarrhoea is a frequent symptom associated with travelling to tropical regions, but the cause is often not found. Epidemiology was assessed including up-to-date real-time PCR approaches.We analysed datasets of 528 patients who presented at the Bernhard Nocht Institute for Tropical Medicine in Hamburg, Germany, between 2006 and 2010 for screening purposes or because of diarrhoea. Stool samples were obtained and investigated by microscopy, bacterial culture, two PCR assays targeting Entamoeba histolytica, Entamoeba dispar, Giardia duodenalis, and Cryptosporidium parvum, or Salmonella spp., Shigella/EIEC spp., Campylobacter jejuni, and Yersinia spp.Among patients with gastrointestinal symptoms, 51% tested positive for bacteria or parasites, of which 66% had a known enteropathogenic potential. In patients without diarrhoea, 53% (n = 80) were positive, and 33% of these cases harboured agents of pathogenic potential. Association with clinical symptoms was primarily found for bacterial infections. Blastocystis hominis, however, was more frequent in asymptomatic than in symptomatic travellers.In conclusion, the study stresses the etiological relevance of bacterial gastroenteritis in travellers returning from the tropics, the need for molecular approaches to increase diagnostic sensitivity and demonstrates that asymptomatic carriage of enteropathogens after prolonged stays in the tropics is similarly frequent compared with symptomatic infections in travellers.

scholarly journals The Prevalence of Blastocystis Infection in Pediatric Patients with Malignancy: A Single-Center Study in Ahvaz, Iran

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Roya Salehi Kahish ◽  
Arash Alghasi ◽  
Shekoufe Hadadi ◽  
Marziyeh Abasi Nasab ◽  
Aida Mafakherzadeh
Keyword(s):  
Clinical Symptoms ◽  
Intestinal Parasites ◽  
Gastrointestinal Symptoms ◽  
Cross Sectional Study ◽  
Parasitic Infections ◽  
Blastocystis Infection ◽  
Cross Sectional ◽  
Patients With Cancer ◽  
Blastocystis Spp ◽  
Life Threatening

Background: Blastocystis spp. is known as one of the few intestinal parasites, prevalent in more than 5% and 30 - 60% of the population in industrialized and developing countries, respectively. In this respect, immunocompromised individuals, such as patients undergoing chemotherapy or those with malignancies, are at risk of the clinical symptoms of Blastocystis infection; however, the given condition is often self-limiting in healthy individuals. Objectives: The current study aimed at evaluating the prevalence of Blastocystis infection in children with malignancies receiving chemo drugs. Methods: The current descriptive, cross-sectional study was conducted on 52 stool specimens collected from patients with cancer admitted to the Oncology Ward of Shahid Baqaei 2 Hospital, Ahvaz, Iran, for six months. A standardized questionnaire was filled out for all cases. Each specimen was also prepared using direct smear, the Lugol iodine staining, and the formalin-ether condensation method. Results: Blastocystis spp. was detected in 21.1% of the cases among them, 11.5% demonstrated gastrointestinal symptoms; therefore, a significant relationship was observed between Blastocystis infection and gastrointestinal symptoms. Conclusion: Patients undergoing chemotherapy should be screened for opportunistic parasitic infections such as Blastocystis to avoid potentially life-threatening outcomes. Besides, further studies are required to identify the subtypes of Blastocystis.

scholarly journals A 5 year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the 5-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P = 0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20–29 years and 40–49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals Cryptosporidiosis among Hemodialysis Patients in Jordan: First Preliminary Screening Surveillance

2019 ◽  
Vol 4 (4) ◽  
pp. 131
Author(s):  
AbdelRahman M. Zueter ◽  
Nawal S. Hijjawi ◽  
Khaled N. Hamadeneh ◽  
Maysa M. Al-Sheyab ◽  
Amal M. Hatamleh
Keyword(s):  
Clinical Symptoms ◽  
Demographic Data ◽  
Cryptosporidium Oocyst ◽  
Gastrointestinal Symptoms ◽  
Staining Technique ◽  
Hemodialysis Patients ◽  
Rural Villages ◽  
Stool Samples ◽  
Immunological Assays ◽  
Brief Screening

Few studies have reported the incidence of cryptosporidiosis among hemodialysis patients worldwide. Currently many molecular and immunological assays have been developed for the sensitive diagnosis of cryptosporidiosis, but still, the microscopic detection of the parasitic infective stage (oocysts) in stool specimens using modified acid stain is regarded as a reliable sensitive technique which is widely used in many clinical labs. In the present study, a total of 133 stool samples were collected from hemodialysis patients and were screened for Cryptosporidium oocyst using formalin-ether concentration and modified acid-fast staining technique. Clinical and demographic data were also collected and analyzed. Cryptosporidium oocysts were recovered in 15/133 (11%) of the investigated hemodialysis patients. The age of patients ranged from 25 to 80 years (mean: 57.84 ± 12.22). Most of the Cryptosporidium-positive cases were recovered from males (73.7%) residing in rural villages in Irbid city (86.6%). The most repeatedly reported symptoms in the Cryptosporidium-positive patients were gastrointestinal symptoms, including diarrhea (15%), nausea (24%), abdominal pain (23%) and bloating (17%), in addition to general fatigue (32%) and weight loss (19%). No statistically significant associations for certain clinical symptoms or risk factors were found. The present study is the first preliminary study in Jordan that provided a brief screening for the incidence of cryptosporidiosis among hemodialysis patients.

scholarly journals Blastocystis hominis and other intestinal parasites in a community of Pitanga City, Paraná State, Brazil

2005 ◽  
Vol 47 (4) ◽  
pp. 213-217 ◽  
Author(s):  
Solange Aparecida Nascimento ◽  
Maria da Luz Ribeiro Moitinho
Keyword(s):  
Giardia Lamblia ◽  
High Frequency ◽  
Intestinal Parasites ◽  
Blastocystis Hominis ◽  
Laboratory Techniques ◽  
Wet Mount ◽  
Routine Basis ◽  
Stool Samples ◽  
Zinc Sulphate Solution ◽  
Prevalent Species

The objective was to estimate the prevalence of Blastocystis hominis, to evaluate the effectiveness of different techniques for its diagnosis as well as to estimate the prevalence of other intestinal parasites in the community of Campo Verde, a district of Pitanga. The work was carried out from August to October 2004. Samples of feces from children and adults were collected and submitted to the techniques of direct wet mount, flotation in zinc sulphate solution, tube sedimentation, sedimentation in formalin-ether and staining by Kinyoun and iron hematoxylin methods. From 181 studied individuals, 128 (70.7%) showed protozoa and/or helminths in stool samples. The most prevalent species were Endolimax nana (33.7%); B. hominis (26.5%); Giardia lamblia (18.2%); Entamoeba coli (17.1%); Ascaris lumbricoides (16.6%); Iodamoeba bütschlii (9.4%); and ancylostomatidae (7.7%). B. hominis was only identified by the techniques of direct wet mount, sedimentation in formalin-ether and staining by iron hematoxylin, though the latter was less sensitive than the other methods. The high frequency of B. hominis demonstrated by this study indicates the need to include laboratory techniques that enable identification of the parasite on a routine basis.

scholarly journals Clinical and serological evidence of Toxocara infection in school children from Morrope District, Lambayeque, Peru

2008 ◽  
Vol 50 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Yrma A. Espinoza ◽  
Pedro H. Huapaya ◽  
William H. Roldán ◽  
Susana Jiménez ◽  
Zhandra Arce ◽  
...  
Keyword(s):  
School Children ◽  
Giardia Lamblia ◽  
Respiratory Symptoms ◽  
Intestinal Parasite ◽  
Serological Evidence ◽  
Hymenolepis Nana ◽  
Positive Serology ◽  
Blastocystis Hominis ◽  
Ocular Manifestations ◽  
Stool Samples

The aim of this study was to estimate the frequency of human toxocariosis in a child population from Morrope district, Lambayeque, Peru. From October to December 2005, 182 school children (96 male and 86 female) were studied. Blood samples were collected for Toxocara ELISA-IgG test and hematological examination. Additionally, stool samples were collected for coproparasitological examination to check cross reactions. We found frequency of positives in 32.4% (59/182) with a significant higher proportion of positivity in male children (p < 0.00001). 71.2% of the children with positive serology (52 male and seven female), were between five and 10 years old, 77.96% had respiratory symptoms, 61.02% had ocular manifestations, 38.98% had hepatic symptoms, 38.98% had mild or moderate eosinophilia, signs statistically associated with seropositivity. 83.5% of studied population had some intestinal parasite, such as: Blastocystis hominis (53.3%), Giardia lamblia (31.3%), Entamoeba coli (29.1%), Entamoeba histolytica/E. dispar (1.1%), Hymenolepis nana (5.49%), and Ascaris lumbricoides (3.3%), but they had not any association with serology results. The ownership of dogs or/and cats were significantly associated with seropositivity to anti-Toxocara antibodies although the presence of such pets within the house was not. In conclusion, clinical and serological evidence of Toxocara infection exists in the studied population.

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: during the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 41.3% laboratory confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected. Intestinal parasite was reported in all age groups in the area but the age group of 10–19 years (44.7%) was more affected. Key words: Intestinal parasite, Trend prevalence, Gondar, Ethiopia

scholarly journals Differential detection ofEntamoeba histolytica,Entamoeba disparandEntamoeba moshkovskiiin faecal samples using nested multiplex PCR in west of Iran

2019 ◽  
Vol 147 ◽  
Author(s):  
Fares Bahrami ◽  
Ali Haghighi ◽  
Ghasem Zamini ◽  
Mohammadbagher Khademerfan
Keyword(s):  
Clinical Symptoms ◽  
Gastrointestinal Symptoms ◽  
Stool Examination ◽  
Entamoeba Dispar ◽  
Faecal Samples ◽  
Northwest Of Iran ◽  
Stool Samples ◽  
Species Specific ◽  
First Time ◽  
The Relationship

AbstractThis study aimed to determine the prevalence ofEntamoeba histolytica,Entamoeba disparandEntamoeba moshkovskii(collectively referred to asEntamoebacomplex), using microscopic and molecular methods in Kurdistan Province, northwest of Iran. The relationship between positiveEntamoebaspecies and clinical symptoms was also investigated. Eight positiveEntamoebacomplex, as well as fourEntamoebacomplex-like isolates, were detected by microscopic stool examination. DNA was extracted from all positive and from 55 randomly selected negative stool samples. PCR was performed using species-specific 18S rRNA primers for theEntamoebacomplex. All positive PCR samples were sequenced. In total, 14 (1.01%) out of 1383 isolates, i.e. 12 microscopy-positive andEntamoebacomplex-like isolates and two out of 55 microscopy-negative isolates, were identified via PCR and sequencing. Overall, 0.58% (8/1383) of the isolates wereE.dispar, 0.14% (2/1383)E.histolytica, 0.07% (1/1383)E.moshkovskiiand 0.22% (3/1383) were mixed ofE.histolyticaandE.dispar. Based on our findings, the prevalence ofE. disparis greater than that ofE. histoltyica. On the other hand, a case ofE. moshkovskiiwas reported for the first time in this region. It seems that some gastrointestinal symptoms may be attributed toEntamoebaspecies.

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