scholarly journals Changes in the Serum Protein Fractions in Goats after Treatment of Natural Gastrointestinal Parasite Infections

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Frederika Chovanová ◽  
Csilla Tóthová ◽  
Róbert Klein ◽  
Oskar Nagy
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Gastrointestinal Parasites ◽  
Protein Fractions ◽  
Dairy Goat ◽  
Total Serum ◽  
Parasitic Infections ◽  
Gastrointestinal Infections ◽  
Γ Globulins ◽  
Antiparasitic Treatment

Gastrointestinal parasitic infections in small ruminants belong to major health problems. The regulation of gastrointestinal infections in goats and the responses developed against them appear to be different from those observed in sheep. In the literature, there is a lack of data on the effect of gastrointestinal parasitic infections on the serum protein profile in goats. Therefore, the aim of the study was to determine the electrophoretic pattern of serum proteins in goats naturally infected with gastrointestinal parasites and to compare the changes in the total serum proteins and serum protein fractions (albumin and α1-, α2-, β-, and γ-globulins) obtained after antihelminthic treatment. Eight adult female goats of the white shorthaired breed from a small dairy goat farm at the age ranging between 3 and 5 years with average body weight 35.4 ± 3.2 kg and body condition score (BCS) from 1.5 to 2.5 were used in the study. The serum proteins in goats were separated into five fractions: albumin, α1- and α2-globulins, and β- and γ-globulins. Significant changes after treatment were found in the relative concentrations of albumin ( P < 0.01 ) and α2- ( P < 0.05 ), β- ( P < 0.001 ), and γ-globulins ( P < 0.01 ), as well as albumin/globulin ratio ( P < 0.01 ). The mean concentration of total serum proteins was, after the antiparasitic treatment, significantly higher. Among the globulin fractions, the γ-globulin fraction contributed most significantly to these changes ( P < 0.001 ). The results presented in the study suggest a significant effect of antiparasitic treatment in goats on the synthesis of blood serum proteins and on the changes of the proportion of serum protein fractions.

scholarly journals Comparison of serum protein electrophoretic pattern in cows and small ruminants

2015 ◽  
Vol 84 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Oskar Nagy ◽  
Csilla Tóthová ◽  
Veronika Nagyová ◽  
Gabriel Kováč
Keyword(s):  
Serum Protein ◽  
Adult Female ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Small Ruminants ◽  
Protein Fractions ◽  
Total Serum Protein ◽  
Total Serum ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

Determination of the physiological electrophoretic patterns in animals is very useful for clinicians in diagnosing healthy and sick animals. The objective of this study was to investigate the serum protein electrophoretic pattern in cows, sheep, and goats in order to evaluate the differences in the size and number of protein fractions between the evaluated ruminant species. Ten adult multiparous high-yielding dairy cows, 10 adult female sheep and 10 adult female goats were included in this study. All the evaluated animals were clinically healthy. Serum was analyzed for total serum protein concentrations, and for the relative and absolute values of protein fractions with calculation of albumin/globulin ratios. Serum protein fractions were separated by zone electrophoresis on buffered agarose gel. Serum protein electrophoresis identified 6 distinct bands, comprising albumin, alpha1- (α1), alpha2- (α2), beta1- (β1), beta2- (β2), and gamma- (γ) globulins in cows. In sheep, serum proteins exhibited 6 fractions: albumin, α1-, α2-, β-, γ1- and γ2-globulins. In goats, serum proteins were separated into 5 fractions: albumin, α1-, α2-, β- and γ-globulins. Significant differences in the relative as well as absolute means were found for the albumin/globulin ratio and most of the protein fractions, except γ-globulins. No significant differences were found in the concentration of total proteins. These results describe the marked species differences in most of serum protein fractions between the evaluated groups of animals, and contribute to the current knowledge about the physiological electrophoretic pattern of serum proteins in ruminants, which can be used for diagnostic purposes.

scholarly journals The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

2017 ◽  
Vol 67 (2) ◽  
pp. 178-190 ◽  
Author(s):  
Csilla Tóthová ◽  
Pavol Mudroň ◽  
Oskar Nagy
Keyword(s):  
Dairy Cows ◽  
Serum Protein ◽  
Serum Proteins ◽  
Inflammatory Diseases ◽  
Post Partum ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Total Serum ◽  
Control Group ◽  
Γ Globulins

Abstract The objective of the study was to evaluate the electrophoretic pattern of serum proteins in dairy cows suffering from various inflammatory diseases, and to study the influence of these diseases on the concentrations of protein fractions. Fourty dairy cows with clinical signs of various inflammatory diseases were used in the study, including cows with post-partum metritis (n=10), mastitis (n=7), and hoof diseases (n=23). The cows were of a black pied Holstein-Friesian breed, Slovak spotted breed and their crossbreeds at the age of 3.5 to 8 years. Fourteen clinically healthy dairy cows were taken as the control group. The cows were blood sampled for the determination of total serum proteins and serum protein fractions. The protein fractions were divided into albumin, α1-, α2-, β1-, β2-, and γ-globulins. In cows with post-partum metritis we found significantly lower concentrations of albumin (P<0.001) and significantly higher values of α1-globulins (P<0.01) compared with healthy animals. Significantly higher concentrations of β1-globulins were observed in cows with mastitis (P<0.001), while the γ-globulin fraction was non-significantly higher. In cows with hoof diseases we recorded significantly lower values of albumin (P<0.001), significantly higher concentrations of α1- and β1-globulins (P<0.001), and non-significantly higher β2- and γ-globulins. Moreover, in the electrophoretic pattern of serum proteins we found β-γ bridging in thirteen cows with hoof diseases. Presented data suggest marked influence of inflammatory diseases on the concentrations of serum protein fractions in dairy cows. However, further investigations are needed to establish the diagnostic utility of serum protein electrophoresis in bovine clinical practice in relation to inflammatory diseases.

scholarly journals The effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern

2012 ◽  
Vol 81 (4) ◽  
pp. 365-370 ◽  
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Herbert Seidel ◽  
Gabriel Kováč
Keyword(s):  
Serum Protein ◽  
Respiratory Diseases ◽  
Clinical Symptoms ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Total Serum Protein ◽  
Total Serum ◽  
Chronic Respiratory Diseases ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

The aim of this study was to evaluate the effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern. Twenty five calves of the Slovak Spotted breed, Low-land Black Spotted breed, and their crossbreeds with clinical symptoms of chronic respiratory diseases were included into this study. Blood serum was analyzed for the total serum protein concentrations, and the relative and absolute values of serum protein fractions. The results obtained in sick animals were compared with those in 29 clinically healthy calves of the same age and breed. In the calves suffering from chronic respiratory diseases, we found significantly (P < 0.001) higher total serum protein concentrations than in healthy calves. In sick calves a marked shift in the concentrations of the most of protein fractions was observed, with significantly higher values of α1-, β1-, β2- and γ-globulins (P < 0.001, P < 0.05, P < 0.001, and P < 0.01, respectively). On the other hand, the concentrations of albumin in the calves with respiratory diseases were significantly (P < 0.001) lower than those measured in healthy ones. The presented results indicate a marked effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern, with a shift in the albumin and globulin concentrations, which could be useful for clinicians for better evaluation of the pathological changes in affected animals.

scholarly journals Changes in the serum protein electrophoretic pattern in lambs during the first month of life

2014 ◽  
Vol 83 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Oskar Nagy ◽  
Csilla Tóthová ◽  
Veronika Nagyová ◽  
Gabriel Kováč ◽  
Ján Pošivák
Keyword(s):  
Serum Protein ◽  
Neonatal Period ◽  
Protein Profile ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Physiological Adaptation ◽  
Total Serum Protein ◽  
Total Serum ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

Studies of the changes in serum protein pattern in the neonatal period in animals are still limited. Therefore, the objective of this study was to evaluate the changes in the concentrations of serum protein fractions in 7 clinically healthy merino lambs (4 males, 3 females) during their first month of life. The first blood sampling was performed before the colostrum intake and then at 1, 2, 7, 14 and 30 days of age. Blood serum was analysed for total serum protein concentrations and for the relative and absolute values of serum protein fractions - albumin, alpha1- (α1), alpha2- (α2), beta- (β), and gamma- (γ) globulins. The results showed a significant effect of age on the serum total protein concentrations and for all the protein fractions. The concentrations of total proteins and γ-globulins increased significantly 1 day after the colostrum intake (P < 0.001); then decreased gradually till the end of the 1st month. An opposite trend was observed in the concentrations of albumin. A significant decrease of values was found 1 day after the intake of colostrum (P < 0.001) with a subsequent progressive increase of values till the end of the evaluated period. The relative concentrations of α1-globulins significantly decreased during the first month of life (P < 0.001). On the other hand, the relative values of α2- and β-globulins increased significantly from birth till the end of the monitored period (P < 0.001). Our results suggest that the serum protein electrophoretic pattern in growing lambs is significantly influenced by the age of the evaluated animal, and this should be taken into consideration when interpreting the serum protein profile. Our findings extend existing knowledge about significant changes in the protein profile associated with the physiological adaptation process in the neonatal period in young animals.

Effects of inanition, semistarvation, and protein-free diets on serum proteins

1964 ◽  
Vol 206 (2) ◽  
pp. 331-334 ◽  
Author(s):  
Henry E. Weimer ◽  
James F. Godfrey
Keyword(s):  
Serum Protein ◽  
Adult Male ◽  
Serum Proteins ◽  
Protein Fractions ◽  
Total Serum ◽  
Sprague Dawley ◽  
Weight Losses ◽  
Sprague Dawley Rats ◽  
Quantitative Changes ◽  
Protein Free Diet

The effects of inanition, semistarvation, and protein-free diets on the partition of serum proteins in adult, male, Sprague-Dawley rats was investigated following weight losses of 12, 26, and 33%. The quantitative changes in the serum protein fractions under the various types of nutritional stress showed significant differences. The albumin, α1- and γ-globulin fractions decreased significantly in the rats on the starvation regimen, while the semistarvation diet caused major alterations in the α1- and ß-globulins. Feeding a protein-free diet elicited the greatest decline in total serum proteins, reflecting the changes in the albumin, α1-, and α2-globulin fractions. The conclusion was drawn that the electrophoretic fractions of serum do not respond in a uniform and nonspecific manner to diverse nutritional stresses.

scholarly journals The Electrophoretic Pattern of Serum Proteins in Sheep with Naturally Acquired Gastrointestinal Nematode Infections

2020 ◽  
Vol 70 (3) ◽  
pp. 316-328
Author(s):  
Oskar Nagy ◽  
Csilla Tóthová ◽  
Róbert Klein ◽  
Frederika Chovanová
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Parasitic Infections ◽  
Fecal Examination ◽  
Mean Values ◽  
Positive Group ◽  
New Findings ◽  
G Ratio

AbstractThe objective of this study was to describe the serum protein pattern in sheep with naturally acquired gastrointestinal parasitosis and to compare the distribution of protein fractions with the results obtained in animals with negative fecal examination results. Fecal and blood samples were taken from twenty-nine sheep positive for nematode eggs and twenty-four animals with negative results of fecal examination. Between the evaluated groups of sheep significant differences were noted in the relative mean values for all protein fractions and for albumin/globulin ratio (p<0.01 and p<0.001). The concentrations of total proteins showed no significant differences between both groups of sheep. The absolute mean values of albumin, α2-globulins and A/G ratio were significantly lower, the mean concentrations of α1-, β-, γ1- and γ2-globulins significantly higher in the nematode positive group of sheep (p<0.01 and p<0.001). In the nematode positive group the protein electrophoretic pattern showed a double α2-zone in three sheep and the γ-globulin zones were characterized by a diffuse higher broad and wide peaks. The presented results indicate that the gastrointestinal parasitic infections in sheep alter the distribution of serum proteins, and suggest their usefulness in animals with unchanged serum protein concentrations. The study brings new findings and extends the knowledge about the metabolic responses and consequences of gastrointestinal parasitic infections in sheep, particularly with regard to alterations in protein metabolism.

Radioprotective Properties of Some Heterocyclic Nitrogenous Compounds Against X-radiation Injury to Serum Proteins in Mice

1969 ◽  
Vol 24 (5) ◽  
pp. 622-630 ◽  
Author(s):  
H. Roushdy ◽  
T. Pierotti ◽  
M. Polverelli
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Intraperitoneal Administration ◽  
Protein Fractions ◽  
Whole Body ◽  
Total Serum ◽  
Nitrogenous Compounds ◽  
X Irradiation ◽  
Quantitative Changes ◽  
Radioprotective Properties

The results obtained from this study suggest the following: The concentration of total serum proteins in mice is changed very little under the various treatments, while protein fractions showed significant alterations.The concentrations of various serum proteins remain almost constant under normal conditions.Intraperitoneal administration of imidazole or benzimidazle at the mentioned doses induces rapid quantitative changes in the serum which are recovered in about 3 days after injection.Whole-body X-irradiation at 750 r creates slow but progressive and persisting serious changes in concentration of serum protein fractions which end in death of animals 8 — 10 days after irradiation.Whole-body X-irradiation of imidazole or benzimidazole protected animals results in quantitative rapid changes in concentration of serum protein fractions for about four days. There after a slow but steady restoration begins. The concentration approaches the normal levels towards the 10th day after irradiation.Imidazole and benzimidazole proved to be good radioprotectants against the effects of X-radiation on serum protein fractions. Benzimidazole seems to surpass imidazole.

Influence of hypoproteinemia on bile proteins

1959 ◽  
Vol 197 (3) ◽  
pp. 558-560 ◽  
Author(s):  
Taketo Katsuki ◽  
Charles G. Johnston ◽  
Charles Koucky
Keyword(s):  
Serum Protein ◽  
Protein Concentration ◽  
Serum Proteins ◽  
Protein A ◽  
Total Serum Protein ◽  
Total Serum ◽  
Blood Proteins ◽  
Additional Function ◽  
Γ Globulins ◽  
Bile Protein

Plasmapheresis was performed in dogs to change the serum protein and bile protein. A low caloric diet and plasmapheresis resulted in a diminished plasma albumin, slightly increased α-globulin, and definitely increased ß- and γ-globulins. Simultaneously, B-fraction (albumin-containing-fraction of bile) decreased while the C-fraction (α-globulin-containing-fraction of bile) and D-fraction (ß- and γ-globulin-containing-fraction of bile) increased. When the plasmapheresis was discontinued and a normal caloric diet supplied, there was a gradual reversal of these changes toward the original levels. This is further indication that B-, C- and D-fraction of bile proteins arise from blood proteins. It is apparent that this is not by simple transudation of proteins from capillaries to bile canaliculi because the smallest protein molecular B-fraction comprises a smaller proportion of bile proteins than of serum proteins, whereas the larger molecular D-fraction comprises a larger proportion of bile proteins than of serum proteins, and yet the total bile protein concentration is always much less than the total serum protein concentration. There must be some mechanism regulating the occurrence of the bile proteins, and this may be an additional function of the hepatic cells.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

CHILDHOOD TUBERCULOSIS: CLINICAL VALUE OF THE ELECTROPHORETIC PATTERN OF THE SERUM PROTEINS

PEDIATRICS ◽  
1961 ◽  
Vol 27 (1) ◽  
pp. 54-67
Author(s):  
Rosa Lee Nemir ◽  
Charlotte Marker Zitrin ◽  
Paraskevi Tsouros ◽  
Enriqueta Melly
Keyword(s):  
Serum Protein ◽  
Tuberculous Meningitis ◽  
Gamma Globulin ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Miliary Tuberculosis ◽  
Protein Fractions ◽  
Reciprocal Relation ◽  
Tuberculous Infection ◽  
Tuberculous Disease

The blood serum protein fractions of 138 children with tuberculosis were analyzed by paper electrophoresis serially over a period of many months. Many manifestations of tuberculous infection were studied. The group was divided into 11 categories ranging from healed or arrested tuberculous disease to various stages of activity. The serum protein fractions were evaluated in terms of prognosis, type of tuberculous disease, effect of intercurrent infection and age of patient. It was found that the greatest changes occurred in the gamma-globulin and albumin fractions in reciprocal relation. With the exception of tuberculous meningitis, the increase in gamma-globulin usually corresponded to the severity of disease. Albumin was correspondingly decreased, and was low even in tuberculous meningitis. Both fractions approached normal levels as the patients improved. Relatively normal readings were found in patients with tuberculosis observation or arrested tuberculosis. The greatest deviation from normal was seen in patients with miliary tuberculosis and those with pleurisy with effusion. Here, the gamma and alpha2-globulins were very high and the serum albumin was low. The alpha2 fraction was elevated in the children with more severe disease, including tuberculous meningitis; with clinical improvement it returned to normal more rapidly than the gamma. A rise in the beta-globulin fraction suggests caseation. Confirmatory evidence was obtained in patients with endobronchial disease, tuberculous adenitis and from the only necropsy in the series. The significant changes in the various fractions are further described and discussed.

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