scholarly journals Validation of Platelet Counting Accuracy With the Celltac F Automated Hematology Analyzer

2005 ◽  
Vol 2005 (4) ◽  
pp. 235-239 ◽  
Author(s):  
Yutaka Nagai ◽  
Hiroshi Kondo ◽  
Noriyuki Tatsumi
Keyword(s):  
Platelet Count ◽  
Clinical Laboratory ◽  
Ethylenediaminetetraacetic Acid ◽  
Reference Method ◽  
Becton Dickinson ◽  
Accurate Analysis ◽  
Visual Method ◽  
Hematology Analyzer ◽  
Reagent Consumption ◽  
Automated Hematology Analyzer

Rapid and accurate analysis of platelet count plays an important role in evaluating hemorrhagic status. Therefore, we evaluated platelet counting performance of a hematology analyzer, Celltac F (MEK-8222, Nihon Kohden Corporation, Tokyo, Japan), that features easy use with low reagent consumption and high throughput while occupying minimal space in the clinical laboratory. All blood samples were anticoagulated with dipotassium ethylenediaminetetraacetic acid (EDTA-2K). The samples were stored at room temperature (18∘C–22∘C) and tested within 4 hours of phlebotomy. We evaluated the counting ability of the Celltac F hematology analyzer by comparing it with the platelet counts obtained by the flow cytometry method that ISLH and ICSH recommended, and also the manual visual method by Unopette (Becton Dickinson Vacutainer Systems). The ICSH/ISLH reference method is based on the fact that platelets can be stained with monoclonal antibodies to CD41 and/or CD61. The dilution ratio was optimized after the precision, coincidence events, and debris counts were confirmed by the reference method. Good correlation of platelet count between the Celltac F and the ICSH/ISLH reference method (r=0.99), and the manual visual method (r=0.93) were obtained. The regressions werey=0.90x+9.0andy=1.11x+8.4, respectively. We conclude that the Celltac F hematology analyzer for platelet counting was well suited to the ICSH/ISLH reference method for rapidness and reliability.

scholarly journals Evaluation of the performance of sysmex XN-3100 automated hematology analyzer regarding the sysmex XE-2100 and microscopic examination

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Said Incir ◽  
Kerim Erhan Palaoglu
Keyword(s):  
Comparative Method ◽  
Reference Method ◽  
Turnaround Time ◽  
Analytical Sensitivity ◽  
Biological Variability ◽  
Comparison Analysis ◽  
Blood Samples ◽  
Hematology Analyzer ◽  
Quality Control Materials ◽  
Automated Hematology Analyzer

AbstractObjectivesWe performed a verification study of the Sysmex XN-3100 hematology analyzer in comparison with the XE-2100 according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) and the International Council for Standardization in Hematology (ICSH).Materials and methodsBlood samples and quality control materials were used for precision. For comparison, we used the current XE-2100 as the comparative method and analyzed 540 blood samples. The Passing-Bablok and Bland-Altman tests were performed according to the CLSI EP09-A3 and a carryover study was performed according to the CLSI H26-A2 guidelines. The flagging performance of the two analyzers was compared, using two experienced laboratory technicians as the reference method.ResultsThe Sysmex XN-3100 demonstrated high levels of precision for most parameters. For the comparison analysis, all parameters, except for MCHC, monocytes and basophils were within the systematic error limits of desirable biological variability criterion (SeDBV). The carryover was less than 0.4% for all parameters. The flagging performance of the XN-3100 was satisfactory and the overall efficiency was high.ConclusionsThe XN-3100 not only showed a strong correlation and agreement with the XE-2100 but also displayed a comparable analytical sensitivity, and increased specificity, which may result in an improved turnaround time and throughpu.

Pseudothrombocytopenia Secondary to the Effects of EDTA in a Dog

2008 ◽  
Vol 44 (2) ◽  
pp. 95-97 ◽  
Author(s):  
Tamara B. Wills ◽  
K. Jane Wardrop
Keyword(s):  
Platelet Count ◽  
Sodium Citrate ◽  
Ethylenediaminetetraacetic Acid ◽  
Pathological Process ◽  
Bleeding Tendency ◽  
Medical Treatments ◽  
Hematology Analyzer ◽  
Clinically Significant

Pseudothrombocytopenia (PTCP) secondary to the effects of ethylenediaminetetraacetic acid (EDTA) has been noted in horses and pigs and should be considered in dogs with moderate thrombocytopenia and no clinical bleeding tendency. This type of pseudothrombocytopenia is not a pathological process by itself, but it can be clinically significant if diagnostics and medical treatments are initiated based on the reported thrombocytopenia. Platelet clumping occurs with EDTA-dependent PTCP, resulting in inaccurate hematology analyzer platelet concentrations. A nontraumatic venipuncture may be sufficient to obtain an accurate platelet count. However, rare cases in the dog may require blood drawn into a different anticoagulant, such as sodium citrate, to help discriminate a true thrombocytopenia from PTCP.

scholarly journals Utility of peripheral blood smear in platelet count estimation

2019 ◽  
Vol 7 (2) ◽  
pp. 434 ◽  
Author(s):  
Vyankatesh T. Anchinmane ◽  
Shilpa V. Sankhe
Keyword(s):  
Platelet Count ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Severe Thrombocytopenia ◽  
Correlation Test ◽  
Hematology Analyzer ◽  
Pearson's Correlation ◽  
Automated Hematology Analyzer ◽  
Count Estimation

Background: There are several methods of platelet count used in hematology laboratory. These methods are manual counting, automated hematology analyzer counting, platelet count estimation by peripheral blood smear (PBS) method etc. Many diseases such as dengue, malaria, pregnancy induced hypertension etc. may leads to severe thrombocytopenia. Timely and precise diagnosis of platelet count plays very crucial role in critical care management of thrombocytopenia cases. The present study was undertaken to estimate platelet counts by PBS method and correlate them with results from automated hematology analyzer method.Methods: Study included one hundred randomly collected blood samples in EDTA anticoagulant vacutainer tubes. Each blood sample was processed for platelet count estimation with automated hematology analyzer and Leishman’s stained PBS examination. The statistical analysis was done by using Pearson's correlation test to access the agreement between both the methods.Results: The Pearson's correlation test showed significant positive correlation for platelet count estimation between both the methods. (r =0.9789).Conclusions: Platelet count estimation by PBS method is reliable and statistically significant when compared to hematology analyzer method. PBS platelet estimation method can be taken as early and rapid procedure for platelet assessment in critical severe thrombocytopenia cases. This method is simple, cheaper and can be done in rural hospital setup where automation is not available.

scholarly journals Dose- and time-related platelet response with apheresis platelet concentrates and pooled platelets

2017 ◽  
Vol 10 (1) ◽  
pp. 44
Author(s):  
Mohammad Mizanur Rahman ◽  
Lutfunnahar Khan ◽  
Debashish Saha
Keyword(s):  
Platelet Count ◽  
The Other ◽  
Platelet Concentrate ◽  
Platelet Concentrates ◽  
Platelet Response ◽  
Other Hand ◽  
Hematology Analyzer ◽  
The Mean ◽  
Automated Hematology Analyzer

<p>This present study was carried out to compare the post-transfusion platelet increment between the apheresis platelet concentrate (n=74) and pooled platelets (n=54). Pre- and post-transfusion platelet count of the recipient were carried out by automated hematology analyzer. In apheresis platelet concentrate group, the mean 24 hours post-transfusion platelet increment was 47 x 10<sup>9</sup>/L which was statistically significant (p&lt;0.001). On the other hand, in pooled platelets group, the mean 24 hours post–transfusions platelet count increment was 11.0 x 10<sup>9</sup>/L which was also statistically significant (p&lt;0.001). This study concluded that the transfusion of apheresis platelet concentrate was more useful than the transfusion of pooled platelets in terms of platelet count increment and requirement of donor.</p>

Detecting Human CD34 + and CD34 - Hematopoietic Stem and Progenitor Cells Using a Sysmex Automated Hematology Analyzer

2004 ◽  
Vol 10 (4) ◽  
pp. 200-205 ◽  
Author(s):  
F. S. WANG ◽  
R. M. ROWAN ◽  
M. CREER ◽  
A. HAY ◽  
M. DORFNER ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hematopoietic Stem ◽  
Human Cd34 ◽  
Hematology Analyzer ◽  
Stem And Progenitor Cells ◽  
Automated Hematology Analyzer

scholarly journals Relative Frequency and Risk Factors of COVID-19 Related Headache in a Sample of Egyptian Population: A Hospital-Based Study

Pain Medicine ◽  
2021 ◽  
Author(s):  
Mona Hussein ◽  
Wael Fathy ◽  
Ragaey A Eid ◽  
Hoda M Abdel-Hamid ◽  
Ahmed Yehia ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Platelet Count ◽  
Relative Frequency ◽  
Clinical Laboratory ◽  
Primary Headache ◽  
Female Gender ◽  
Imaging Data ◽  
Laboratory Findings ◽  
Neutrophil Lymphocyte Ratio ◽  
The Impact

Abstract Objectives Headache is considered one of the most frequent neurological manifestations of coronavirus disease 2019 (COVID-19). This work aimed to identify the relative frequency of COVID-19-related headache and to clarify the impact of clinical, laboratory findings of COVID-19 infection on headache occurrence and its response to analgesics. Design Cross-sectional study. Setting Recovered COVID-19 patients. Subjects In total, 782 patients with a confirmed diagnosis of COVID-19 infection. Methods Clinical, laboratory, and imaging data were obtained from the hospital medical records. Regarding patients who developed COVID-19 related headache, a trained neurologist performed an analysis of headache and its response to analgesics. Results The relative frequency of COVID-19 related headache among our sample was 55.1% with 95% confidence interval (CI) (.516–.586) for the estimated population prevalence. Female gender, malignancy, primary headache, fever, dehydration, lower levels of hemoglobin and platelets and higher levels of neutrophil/lymphocyte ratio (NLR) and CRP were significantly associated with COVID-19 related headache. Multivariate analysis revealed that female gender, fever, dehydration, primary headache, high NLR, and decreased platelet count were independent predictors of headache occurrence. By evaluating headache response to analgesics, old age, diabetes, hypertension, primary headache, severe COVID-19, steroid intake, higher CRP and ferritin and lower hemoglobin levels were associated with poor response to analgesics. Multivariate analysis revealed that primary headache, steroids intake, moderate and severe COVID-19 were independent predictors of non-response to analgesics. Discussion Headache occurs in 55.1% of patients with COVID-19. Female gender, fever, dehydration, primary headache, high NLR, and decreased platelet count are considered independent predictors of COVID-19 related headache.

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