Protective effect of guava leaf extract on colonic tissues with ulcerative colonitis induced by trinitrobenzene sulfonic acid in rats

This study was carried out to evaluate the protective effect of anthocyanins extract of blueberry on trinitrobenzene sulfonic acid (TNBS)-induced inflammatory bowel disease (IBD) model of mice. The study employed female C57BL/6 mice (n= 50), and colitis was induced by intracolonic injection of 0.5 mg of TNBS dissolved in 50% ethanol–phosphate buffered solution. The mice were divided into five groups (n= 10): vehicle, TNBS control and anthocyanins groups that received different doses of anthocyanins extract (10, 20 and 40 mg kg-1) daily for 6 days. Both increase in body weight and diarrhea symptoms were monitored each day. After 6 days, the animals were killed, and the following parameters were assessed: colon length, morphological score, histological score and biochemical assay (NO, myeloperoxidase (MPO), interleukin (IL)-12, IL-10, tumor necrosis factor (TNF)-αand interferon (IFN)-γ). The results showed that the anthocyanins extract of blueberry rendered strong protection against TNBS-induced colonic damage at a dosage of 40 mg kg-1. When compared with the control, anthocyanins extract significantly prevented loss of body weight and ameliorated the scores of diarrhea, morphology and histology. Treatment with anthocyanins extract restored IL-10 excretion, as well as caused reduction in the levels of NO, MPO, IL-12, TNF-αand IFN-γ. Our research revealed the protective effect of anthocyanins extract from blueberry on TNBS-induced experimental colitis in mice, as well as examined whether high levels of dietary blueberries would lower the risk or have protective effects on human IBD, which may require further investigation.

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Protective Effect of Daidzein on TNBS Induced Acute Ulcerative Colitis on Rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4348 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 1627-1635

Author(s):

Mohammed Nadeem Zahed ◽

Kavitha CH N

Keyword(s):

Ulcerative Colitis ◽

Protective Effect ◽

Sulfonic Acid ◽

Reduced Glutathione ◽

Nitrate Content ◽

Trinitrobenzene Sulfonic Acid ◽

Colon Injury ◽

Tnf Α ◽

Descending Colon ◽

Per Oral

The present study aimed at investigating the potential protective effect of Daidzein on 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced acute ulcerative colitis in rats. Animals were treatment with TNBS 20 mg, TNBS dissolved in 50% ethanol by single intra-colonic application into the descending colon, Daidzein 40 and 80 mg/kg per oral, and standard sulfasalazine (SSZ) 360 mg/kg. for Two weeks. Colon was removed, length (CL), weight (CW), microscopic index (MI) processed for histopathological evaluation and estimation of oxidative colon marker contents of active lipid peroxidation (MDA) myeloperoxidase (MPO), reduced glutathione (GSH). Enzymatic activity of superoxide dismutase (SOD) and serum nitrate levels were assessed. TNBS induced significant (p < 0.001), increase in CW, MI, oxidative marker MDA, MPO, and serum nitrate content, TNBS induced a significantly decrease in CL, SOD, and GSH content. Treatment of Daidzein 40 and 80mg with TNBS decreased preserved colon parameter and histology close to normal, increased (P<0.001) SOD, CAT, GSH and TNF- α IL-6 and IL-8 down-regulate the levels to compare SSZ and Daidzein. Daidzein 40and 80mg restored TNBS-induced colon injury via inhibition of oxidative stress. Daidzein found to protect the TNBS Induced Acute Ulcerative Colitis in rats.

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