scholarly journals Protective Effect of Daidzein on TNBS Induced Acute Ulcerative Colitis on Rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1627-1635
Author(s):  
Mohammed Nadeem Zahed ◽  
Kavitha CH N
Keyword(s):  
Ulcerative Colitis ◽  
Protective Effect ◽  
Sulfonic Acid ◽  
Reduced Glutathione ◽  
Nitrate Content ◽  
Trinitrobenzene Sulfonic Acid ◽  
Colon Injury ◽  
Tnf Α ◽  
Descending Colon ◽  
Per Oral

The present study aimed at investigating the potential protective effect of Daidzein on 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced acute ulcerative colitis in rats. Animals were treatment with TNBS 20 mg, TNBS dissolved in 50% ethanol by single intra-colonic application into the descending colon, Daidzein 40 and 80 mg/kg per oral, and standard sulfasalazine (SSZ) 360 mg/kg. for Two weeks. Colon was removed, length (CL), weight (CW), microscopic index (MI) processed for histopathological evaluation and estimation of oxidative colon marker contents of active lipid peroxidation (MDA) myeloperoxidase (MPO), reduced glutathione (GSH). Enzymatic activity of superoxide dismutase (SOD) and serum nitrate levels were assessed. TNBS induced significant (p < 0.001), increase in CW, MI, oxidative marker MDA, MPO, and serum nitrate content, TNBS induced a significantly decrease in CL, SOD, and GSH content. Treatment of Daidzein 40 and 80mg with TNBS decreased preserved colon parameter and histology close to normal, increased (P<0.001) SOD, CAT, GSH and TNF- α IL-6 and IL-8 down-regulate the levels to compare SSZ and Daidzein. Daidzein 40and 80mg restored TNBS-induced colon injury via inhibition of oxidative stress. Daidzein found to protect the TNBS Induced Acute Ulcerative Colitis in rats.

scholarly journals Study on the effect of Periplaneta americana on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid

2020 ◽  
Vol 18 ◽  
pp. 205873922094262
Author(s):  
Yi-Hao Che ◽  
Zhi-Bin Yang ◽  
Han-Chao Zhang ◽  
Xiu-Mei Wu ◽  
Min-Zhe Sun ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Sulfonic Acid ◽  
Periplaneta Americana ◽  
Treated Group ◽  
Normal Control Group ◽  
Control Group ◽  
Trinitrobenzene Sulfonic Acid ◽  
Tnf Α ◽  
Ifn Γ ◽  
Tgf Β1

Ulcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, and Periplaneta americana has been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect of Periplaneta americana extract Ento-A on UC in rats induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its mechanism. The Sprague-Dawley (SD) rats were randomly divided into normal control group; TNBS-treated group; sulfasalazine (SASP) treated group; Ento-A low- (50 mg/kg), medium- (100 mg/kg), and high-dose (200 mg/kg) groups, respectively. The UC model of rats was induced via TNBS. Disease activity index (DAI) was used to evaluate the severity of UC in rats. The macroscopic and microscopic damages of colon were accessed by colon mucosa damage index (CMDI) and histopathological score (HS), respectively. The levels of interleukin-4 (IL-4), interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in serum and the contents of myeloperoxidase (MPO), transforming growth factor-β1 (TGF-β1), and epidermal growth factor (EGF) in colonic mucosa were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the normal control group, the TNBS-treated group showed increase in DAI, CMDI, HS, IL-17, TNF-α, IFN-γ as well as MPO and decrease in the levels of IL-4, EGF, and TGF-β1. However, Ento-A-administrated groups reversed the changes in the DAI, CMDI, HS, and the cytokines caused by TNBS. The study indicates that Periplaneta americana extract Ento-A can effectively alleviate the inflammation in TNBS-induced UC of rats, and the mechanism of that may be related to restoring the balance of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cytokines.

scholarly journals Mouse model of ulcerative colitis using trinitrobenzene sulfonic acid

2015 ◽  
Vol 10 (4) ◽  
pp. 860
Author(s):  
Irfan Ahmad Rather ◽  
Vivek K. Bajpai ◽  
Nam Gyeong-Jun
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Mouse Model ◽  
Bowel Disease ◽  
Sulfonic Acid ◽  
Intestinal Inflammation ◽  
Cost Effective ◽  
Trinitrobenzene Sulfonic Acid ◽  
Clinical Presentations ◽  
Inflammatory Bowel

<p>Animal model of intestinal inflammation is of paramount significance that aids in discerning the pathologies underlying ulcerative colitis and Crohn’s disease, the two clinical presentations of inflammatory bowel disease. The 2,4,6-trinitrobenzene sulfonic acid (TNBS) colitis model represents one such intestinal inflammation-prototype that is generated in susceptible strains of mice through intra-rectal instillation of compound TNBS. In this paper, we demonstrate the experimental induction of TNBS-mediated colitis in a susceptible strain of ICR mice. This can be done by the following steps: a) acclimation, b) induction and c) observation. TNBS-mouse model provides the information in shortest possible time and simultaneously represents a cost effective and highly reproducible model method of studying the pathogenesis of inflammatory bowel disease.</p><p><strong>Video Clips</strong></p><p><a href="https://youtube.com/v/6MsuIGzH3uA">Acclimation and induction of TNBS</a>:          4.5 min</p><p><a href="https://youtube.com/v/ya66SNwoVag">Observation and drug administration</a>:      1.5 min</p>

The effect of memantine on trinitrobenzene sulfonic acid-induced ulcerative colitis in mice

2016 ◽  
Vol 793 ◽  
pp. 28-34 ◽  
Author(s):  
Ehsan Motaghi ◽  
Valiollah Hajhashemi ◽  
Parvin Mahzouni ◽  
Mohsen Minaiyan
Keyword(s):  
Ulcerative Colitis ◽  
Sulfonic Acid ◽  
Trinitrobenzene Sulfonic Acid

scholarly journals Protective Effect of Anthocyanins Extract from Blueberry on TNBS-Induced IBD Model of Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Lin-Hua Wu ◽  
Zeng-Lai Xu ◽  
Di Dong ◽  
Shan-An He ◽  
Hong Yu
Keyword(s):  
Body Weight ◽  
Protective Effect ◽  
Sulfonic Acid ◽  
Experimental Colitis ◽  
Trinitrobenzene Sulfonic Acid ◽  
Protective Effects ◽  
Histological Score ◽  
Inflammatory Bowel ◽  
Necrosis Factor ◽  
Different Doses

This study was carried out to evaluate the protective effect of anthocyanins extract of blueberry on trinitrobenzene sulfonic acid (TNBS)-induced inflammatory bowel disease (IBD) model of mice. The study employed female C57BL/6 mice (n= 50), and colitis was induced by intracolonic injection of 0.5 mg of TNBS dissolved in 50% ethanol–phosphate buffered solution. The mice were divided into five groups (n= 10): vehicle, TNBS control and anthocyanins groups that received different doses of anthocyanins extract (10, 20 and 40 mg kg-1) daily for 6 days. Both increase in body weight and diarrhea symptoms were monitored each day. After 6 days, the animals were killed, and the following parameters were assessed: colon length, morphological score, histological score and biochemical assay (NO, myeloperoxidase (MPO), interleukin (IL)-12, IL-10, tumor necrosis factor (TNF)-αand interferon (IFN)-γ). The results showed that the anthocyanins extract of blueberry rendered strong protection against TNBS-induced colonic damage at a dosage of 40 mg kg-1. When compared with the control, anthocyanins extract significantly prevented loss of body weight and ameliorated the scores of diarrhea, morphology and histology. Treatment with anthocyanins extract restored IL-10 excretion, as well as caused reduction in the levels of NO, MPO, IL-12, TNF-αand IFN-γ. Our research revealed the protective effect of anthocyanins extract from blueberry on TNBS-induced experimental colitis in mice, as well as examined whether high levels of dietary blueberries would lower the risk or have protective effects on human IBD, which may require further investigation.

Sign in / Sign up

Export Citation Format

Share Document