Role of LKB1 in proliferation and apoptosis of gastric cancer cells

Abstract Background Recently, tRNA-derived fragments (tRFs) have been shown to serve important biological functions. However, the role of tRFs in gastric cancer has not been fully elucidated. This study aimed to identify the tumor suppressor role of tRF-5026a (tRF-18-79MP9P04) in gastric cancer. Methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was first used to detect tRF-5026a expression levels in gastric cancer tissues and patient plasma. Next, the relationship between tRF-5026a levels and clinicopathological features in gastric cancer patients was assessed. Cell lines with varying tRF-5026a levels were assessed by measuring tRF-5026a using qRT-PCR. After transfecting cell lines with a tRF-5026a mimic or inhibitor, cell proliferation, colony formation, migration, apoptosis, and cell cycle were evaluated. The expression levels of related proteins in the PTEN/PI3K/AKT pathway were also analyzed by Western blotting. Finally, the effect of tRF-5026a on tumor growth was tested using subcutaneous tumor models in nude mice. Results tRF-5026a was downregulated in gastric cancer patient tissues and plasma samples. tRF-5026a levels were closely related to tumor size, had a certain diagnostic value, and could be used to predict overall survival. tRF-5026a was also downregulated in gastric cancer cell lines. tRF-5026a inhibited the proliferation, migration, and cell cycle progression of gastric cancer cells by regulating the PTEN/PI3K/AKT signaling pathway. Animal experiments showed that upregulation of tRF-5026a effectively inhibited tumor growth. Conclusions tRF-5026a (tRF-18-79MP9P04) is a promising biomarker for gastric cancer diagnostics and has tumor suppressor effects mediated through the PTEN/PI3K/AKT signaling pathway.

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SLCO4A1-AS1 Facilitates the Malignant Phenotype via miR-149-5p/STAT3 Axis in Gastric Cancer Cells

Journal of Oncology ◽

10.1155/2021/1698771 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Qing Li ◽

Dachuan Zhang ◽

Hui Wang ◽

Jun Xie ◽

Lei Peng ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Organic Anion ◽

Organic Anion Transporter ◽

Gastric Cancer Cells ◽

Anion Transporter ◽

Transporter Family ◽

Cancer Tissues ◽

Development Of Gastric Cancer

Solute carrier organic anion transporter family member 4A1 (SLCO4A1-AS1), a newly discovered lncRNA, may exert effects in tumors. Since its role in gastric cancer remains obscure, we sought to explore the mechanism of SLCO4A1-AS1 in gastric cancer. The relationship among SLCO4A1-AS1, miR-149-5p, and STAT3 was detected by bioinformatics, dual luciferase analysis, and Pearson’s test, and the expressions of these genes were determined by quantitative real-time PCR and Western blot. Moreover, CCK-8, flow cytometry, wound healing assay, and Transwell analysis were performed to verify the function of SLCO4A1-AS1 in gastric cancer. Rescue experiments were used to detect the role of miR-149-5p. The expressions of SLCO4A1-AS1 and STAT3 were increased, while the expression of miR-149-5p was suppressed in gastric cancer tissues and cell lines. In addition, STAT3 expression was negatively correlated with miR-149-5p expression but was positively correlated with SLCO4A1-AS1 expression. Overexpression of SLCO4A1-AS1 promoted cell viability, migration, invasion, and STAT3 expression but suppressed apoptosis, while knockdown of SLCO4A1-AS1 had the opposite effect. SLCO4A1-AS1 bound to miR-149-5p and targeted STAT3. Moreover, miR-149-5p mimic inhibited the malignant development of gastric cancer cells and obviously reversed the function of SLCO4A1-AS1 overexpression. Our research reveals that abnormally increased SLCO4A1-AS1 expression may be an important molecular mechanism in the development of gastric cancer.

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Functional Role of -Adrenergic Receptors in the Mitogenic Action of Nicotine on Gastric Cancer Cells

Toxicological Sciences ◽

10.1093/toxsci/kfl118 ◽

2006 ◽

Vol 96 (1) ◽

pp. 21-29 ◽

Cited By ~ 50

Author(s):

V. Y. Shin ◽

W. K. K. Wu ◽

K. M. Chu ◽

M. W. L. Koo ◽

H. P. S. Wong ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Adrenergic Receptors ◽

Functional Role ◽

Gastric Cancer Cells ◽

Mitogenic Action

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Effects of Amygdalin on TLR4/NF-αB Signaling Pathway-Mediated Proliferation and Apoptosis of Gastric Cancer Cells

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.20:153-158 ◽

2021 ◽

Vol 20 (1) ◽

pp. 153-158

Author(s):

Abulajiang Abudoukelimu ◽

Xinhui Yang ◽

Lei Ge ◽

Xiangyue Zeng ◽

Yin Shu ◽

...

Keyword(s):

Gastric Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

B Cell Lymphoma ◽

Toll Like Receptor ◽

Gastric Cancer Cells ◽

Toll Like Receptor 4 ◽

Proliferation And Apoptosis ◽

High Incidence Rate ◽

Factor Α

Gastric cancer is a highly malignant tumor of the digestive tract with high incidence rate and mortality. In the present study, we have shown decreased cell proliferation, increased apoptosis, and expression of proinflammatory cytokines (Interleukin-6, Interleukin-1β, and Tumor necrosis factor-α) in gastric cancer cells MGC-803 by amygdalin. Also, amygdalin treatment significantly reduced expression of the mRNA and protein for B-cell lymphoma 2 protein, CyclinD1, toll-like receptor 4, and REL-associated protein involved in NF-κB heterodimer formation in MGC-803 cells. In summary, amygdalin inhibits the proliferation of gastric cancer cells MGC-803 and promotes cell apoptosis by regulating the toll-like receptor 4/ nuclear factor-kappa B signaling pathway.

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