scholarly journals Down-regulation of Integrin α2 Surface Expression by Mutant Epidermal Growth Factor Receptor (EGFRvIII) Induces Aberrant Cell Spreading and Focal Adhesion Formation

2005 ◽  
Vol 65 (20) ◽  
pp. 9280-9286 ◽  
Author(s):  
Yan Ning ◽  
Reema Zeineldin ◽  
Yueying Liu ◽  
Martina Rosenberg ◽  
M. Sharon Stack ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Focal Adhesion ◽  
Adhesion Formation ◽  
Growth Factor Receptor ◽  
Cell Spreading ◽  
Surface Expression ◽  
Focal Adhesion Formation ◽  
Epidermal Growth ◽  
Integrin Α2

scholarly journals Spatial activation of ezrin by epidermal growth factor receptor and focal adhesion kinase co-ordinates epithelial cell migration

Open Biology ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 210166
Author(s):  
Grace K. Chan ◽  
John A. McGrath ◽  
Maddy Parsons
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Epidermal Growth Factor ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Growth Factor Receptor ◽  
Adaptor Protein ◽  
Epidermal Growth ◽  
And Migration

Epidermal growth factor receptor (EGFR) plays a critical role in the promotion of epithelial cell proliferation and migration. Previous studies have suggested a cooperative role between EGFR and integrin signalling pathways that enable efficient adhesion and migration but the mechanisms controlling this remain poorly defined. Here, we show that EGFR forms a complex with focal adhesion kinase in epithelial cells. Surprisingly, this complex enhances local Src activity at focal adhesions to promote phosphorylation of the cytoskeletal adaptor protein ezrin at Y478, leading to actomyosin contractility, suppression of focal adhesion dynamics and slower migration. We further demonstrate this regulation of Src is due to the suppression of PTP1B activity. Our data provide new insight into EGF-independent cooperation between EGFR and integrins and suggest transient interactions between these kinases at the leading edge of cells act to spatially control signalling to permit efficient motility.

scholarly journals Phenotypic heterogeneity in the NCI-H125 cell line affects biological activity using the epidermal growth factor receptor as target / Fenotipska heterogenost u staničnoj liniji NCI-H125 utječe na biološku aktivnost na receptore za epidermalni faktor rasta

2012 ◽  
Vol 62 (4) ◽  
pp. 581-591
Author(s):  
Rancés Blanco ◽  
Mercedes Cedeño ◽  
Narjara González ◽  
Reynier Rodríguez ◽  
Javier Sánchez ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Epidermal Growth Factor Receptor ◽  
Biological Activity ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Viability ◽  
Cell Cultures ◽  
Growth Factor Receptor ◽  
Surface Expression ◽  
Epidermal Growth

We evaluated the influence of some morphological changes of the NCI-H125 cell line in surface expression of the epidermal growth factor receptor (EGFR) and their impact on some biological activity assays using this molecule as target. Hematoxylin and eosin (H/E) staining, light microscopy immunocytochemistry, flow cytometric antibody-receptor binding test, cell viability determination and cell cycle analysis were performed. Phenotypic changes and inconsistency in EGFR expression were detected in NCI-H125 cell cultures. A significant reduction in the growth rate, mainly characterized by cell cycle arrest in the G0-G1 phase, was also evidenced. Differential distribution of cell viability in NCI-H125 subpopulations and its relationship with the EGFR surface expression were determined. Nuclear alterations observed in NCI- -H125 were not apoptosis related. A lack of control of cell cultures affects the reliability and reproducibility of biomedical and biotechnological research using EGFR as target. Therefore, rigorous control of the above mentioned parameters in these experiments is recommended.

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