Abstract P3-12-20: The impact of everolimus on radiation-induced pulmonary fibrosis in wistar albino rats: Results of an experimental study

Abstract Since the early 1940s, a significant amount of research has been conducted to describe the impact of the high-G acceleration on the cardiovascular system. The objective of the present study was to examine the role of the antioxidant enzyme system under biodynamic stress in the liver, heart and gastric mucosa in response to high-magnitude +Gz exposure in a rat model. Twenty adult male Wistar albino rats (10 rats per group; 9-11 weeks old, 200-250 g b.w.) were divided into the following two groups: control and G (exposed to a biodynamic stress model under positive (+7 Gz) acceleration for 40 s). The influence of acute biodynamic stress on pro-oxidative parameters in the rat liver (xanthine oxidase (XOD), catalase (CAT), peroxidase (Px), glutathione peroxidase (GSH-Px), total content of glutathione (GSH), lipid peroxidation (LPx)) and on histopathological alterations in the liver, cardiac muscle and gastric mucosa was examined. Biodynamic stress resulting from positive (+7 Gz) acceleration resulted in a highly statistically signifi cant increase of CAT GSH-Px activity compared to the control group. The LPx levels were significantly decreased, but the GSH contents and the activities of other enzymes were not significantly changed. Significant microscopic changes in the liver, heart and gastric mucosa were observed in the G group. These results clearly indicate that +Gz acceleration alters biochemical systems. These alterations in cellular processes may be mediated by influences of hypoxia or ischaemia via changes in the antioxidant capacity.

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The Neuroprotective and Anti-Inflammatory Effects of Annona Muricata (Graviola) on Radiation-Induced Rat Sciatic Nerve Injury.

10.21203/rs.3.rs-804331/v1 ◽

2021 ◽

Author(s):

Emrah Keskin ◽

Özlem Elmas ◽

Havva Hande Keser Şahin ◽

Çağhan Tönge ◽

Ahmet Günaydın

Keyword(s):

Sciatic Nerve ◽

Ionizing Radiation ◽

Nerve Injury ◽

Sciatic Nerve Injury ◽

Albino Rats ◽

Annona Muricata ◽

Leaf Extracts ◽

Normal Tissues ◽

Radiation Induced ◽

Wistar Albino Rats

Abstract Background: In radiotherapy (RT) exposure area, normal tissues are also affected that may cause serious complications in the patients. This study aimed to evaluate Annona muricata’s radioprotective effects on sciatic nerve injury due to ionizing radiation (IR). Methods and Results: 32 adult female Wistar albino rats separated into 4 equal groups; Control (C), Annona muricata leaf extracts (AME), radiation (RAD), radiation and Annona muricata leaf extracts (AME+RAD). In groups AME and AME+RAD, Annona muricata leaf extracts were administered at a dose of 300 mg/kg for the first day and 50 mg/kg everyday for following one week intraperitoneally. In RAD and AME+RAD, rats were exposed to a single dose of 20 Gray IR to their right legs. All the subjects were sacrified at the end of the first month. Oxidative stress biochemical parameters (SOD, CAT and GPx) from blood samples were analyzed. Right sciatic nerves extracted and histomorphology evaluated. Statistically significant vasculature, degenerative and necrotic changes were observed in RAD, compared to C and AME (p<0,01). Swelling in myelin sheath was predominantly seen in RAD. Alterations in the level of CAT (p<0,01), SOD (p<0,01) and GPx (p<0,05) in AME+RAD group compared to RAD group were found to be statistically significant.Conclusion: Our study unveiled that AM could have a potential of biochemically and histomorphology healing on sciatic nerve injury due to ionizing radiation.

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An Experimental Study to Evaluate the Anti-Diarrheal Activity of Bhuvaneshwara Rasa in Wistar Albino Rats

International Journal of Ayurveda and Pharma Research ◽

10.47070/ijapr.v9i9.2074 ◽

2021 ◽

pp. 27-35

Author(s):

Simi Jose ◽

Chaitra L.V

Keyword(s):

Experimental Study ◽

Castor Oil ◽

Raw Materials ◽

Life Time ◽

Drug Group ◽

Albino Rats ◽

Test Drug ◽

Wistar Albino Rats ◽

Important Disease

Atisara (Diarrhea) is most commonly encountered disease of the present era, due to unhealthy and irregular habits. Atisara (Diarrhea) finds a place as important disease in individual’s life as everyone suffers from it at least once in life time. Bhuvaneshwara rasa is a unique herbo-mineral formulation explained in Bhaishajya ratnavali indicated for all kinds of diarrhea cases. Bhuvaneshwara rasa is a potent formulation having Saindhava, Triphala, Yamani, Bilva peshika and Gruhadhooma. Materials and methods: Raw materials were screened and collected and the formulation selected for the present study Bhuvaneshwara rasa was prepared accordingly. Anti-diarrheal activity of Bhuvaneshwara rasa was evaluated experimentally in albino rats. Experimental study was conducted in 3 groups of animals for anti-diarrheal study each. Anti-diarrheal study was done by castor oil induced diarrhoea and castor oil induced enter-pooling method. Bhuvaneshwara rasa (test drug) and Loperamide are effective in controlling diarrheal episodes. Bhuvaneshwara rasa has shown significant Anti-diarrheal activity in both Castor oil -induced diarrhea and castor oil induced enteropooling, test drug effective than control. Result: Bhuvaneshwara rasa was prepared according to SOP. Test drug group have shown effect experimentally. Conclusion: Bhuvaneshwara rasa is a good anti-diarrheal drug. It can be administered in all types of Atisara.

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