Abstract A72: VAP-1 expression is significantly reduced in colorectal cancer compared to normal colon tissue despite elevated sVAP-1 levels.

2013 ◽  
Author(s):  
Stephen T. Ward ◽  
Christopher J. Weston ◽  
Emma L. Shepherd ◽  
Elizabeth A. Hepburn ◽  
Ka-kit Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Tissue

scholarly journals Genotype-Based Gene Expression in Colon Tissue—Prediction Accuracy and Relationship with the Prognosis of Colorectal Cancer Patients

2020 ◽  
Vol 21 (21) ◽  
pp. 8150
Author(s):  
Heike Deutelmoser ◽  
Justo Lorenzo Bermejo ◽  
Axel Benner ◽  
Korbinian Weigl ◽  
Hanla A. Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Prediction Accuracy ◽  
Inverse Association ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Tissue ◽  
Study Population ◽  
The Uk ◽  
Colorectal Cancer Patients

Colorectal cancer (CRC) survival has environmental and inherited components. The expression of specific genes can be inferred based on individual genotypes—so called expression quantitative trait loci. In this study, we used the PrediXcan method to predict gene expression in normal colon tissue using individual genotype data from 91 CRC patients and examined the correlation ρ between predicted and measured gene expression levels. Out of 5434 predicted genes, 58% showed a negative ρ value and only 16% presented a ρ higher than 0.10. We subsequently investigated the association between genotype-based gene expression in colon tissue for genes with ρ > 0.10 and survival of 4436 CRC patients. We identified an inverse association between the predicted expression of ARID3B and CRC-specific survival for patients with a body mass index greater than or equal to 30 kg/m2 (HR (hazard ratio) = 0.66 for an expression higher vs. lower than the median, p = 0.005). This association was validated using genotype and clinical data from the UK Biobank (HR = 0.74, p = 0.04). In addition to the identification of ARID3B expression in normal colon tissue as a candidate prognostic biomarker for obese CRC patients, our study illustrates the challenges of genotype-based prediction of gene expression, and the advantage of reassessing the prediction accuracy in a subset of the study population using measured gene expression data.

Epidermal growth factor (EGF) A61G polymorphism and EGF gene expression in normal colon tissue from patients with colorectal cancer

2007 ◽  
Vol 46 (8) ◽  
pp. 1113-1117 ◽  
Author(s):  
Karen-Lise G. Spindler ◽  
Jens N. Nielsen ◽  
Dorthe Ornskov ◽  
Ivan Brandslund ◽  
Anders Jakobsen
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Tissue ◽  
Epidermal Growth

Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples

2020 ◽  
Vol 493 (1) ◽  
pp. 208-210
Author(s):  
M. Yu. Shkurnikov ◽  
S. A. Nersisyan ◽  
A. Sh. Osepyan ◽  
D. V. Maltseva ◽  
E. N. Knyazev
Keyword(s):  
Colorectal Cancer ◽  
Normal Colon ◽  
Colon Tissue ◽  
Tissue Samples ◽  
Normal Colon Tissue ◽  
Dicer Cleavage

Cold atmospheric plasma treatment inhibits growth in colorectal cancer cells

2018 ◽  
Vol 400 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Christin Schneider ◽  
Stephanie Arndt ◽  
Julia L. Zimmermann ◽  
Yangfang Li ◽  
Sigrid Karrer ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Plasma Treatment ◽  
Cancer Cells ◽  
Ex Vivo ◽  
Atmospheric Plasma ◽  
Normal Colon ◽  
Colon Tissue ◽  
Cold Atmospheric Plasma ◽  
Colorectal Cancer Cells

AbstractPlasma oncology is a relatively new field of research. Recent developments have indicated that cold atmospheric plasma (CAP) technology is an interesting new therapeutic approach to cancer treatment. In this study, p53 wildtype (LoVo) and human p53 mutated (HT29 and SW480) colorectal cancer cells were treated with the miniFlatPlaSter – a device particularly developed for the treatment of tumor cells – that uses the Surface Micro Discharge (SMD) technology for plasma production in air. The present study analyzed the effects of plasma on colorectal cancer cellsin vitroand on normal colon tissueex vivo. Plasma treatment had strong effects on colon cancer cells, such as inhibition of cell proliferation, induction of cell death and modulation of p21 expression. In contrast, CAP treatment of murine colon tissueex vivofor up to 2 min did not show any toxic effect on normal colon cells compared to H2O2positive control. In summary, these results suggest that the miniFlatPlaSter plasma device is able to kill colorectal cancer cells independent of their p53 mutation status. Thus, this device presents a promising new approach in colon cancer therapy.

scholarly journals DNA demethylation in normal colon tissue predicts predisposition to multiple cancers

Oncogene ◽  
2012 ◽  
Vol 31 (48) ◽  
pp. 5029-5037 ◽  
Author(s):  
H Kamiyama ◽  
K Suzuki ◽  
T Maeda ◽  
K Koizumi ◽  
Y Miyaki ◽  
...  
Keyword(s):  
Dna Demethylation ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Tissue ◽  
Multiple Cancers

scholarly journals Hyper-Elongation in Colorectal Cancer Tissue – Cerotic Acid is a Potential Novel Serum Metabolic Marker of Colorectal Malignancies

2017 ◽  
Vol 41 (2) ◽  
pp. 722-730 ◽  
Author(s):  
Adriana Mika ◽  
Jaroslaw Kobiela ◽  
Aleksandra Czumaj ◽  
Michał Chmielewski ◽  
Piotr Stepnowski ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tissue Expression ◽  
Gas Chromatography Mass Spectrometry ◽  
Cancer Tissue ◽  
Colon Mucosa ◽  
Normal Colon ◽  
Colon Tissue ◽  
Normal Colon Mucosa ◽  
Metabolic Marker ◽  
Cerotic Acid

Backgrounds/Aims: Colorectal cancer (CRC) cells show some alterations of lipid metabolism. Elongation of fatty acids (FA) has not been studied in CRC tissues thus far. The aim of this study was to verify if CRC specimens and normal colon mucosa differ in terms of their levels of very long-chain FAs, a product of FA elongation. Moreover, the expression of elongase genes has been studied in normal tissue and CRC. Finally, we searched for some specific products of FA elongation in serum of CRC patients. Methods: The specimens of normal colon mucosa and CRC were obtained from nineteen CRC patients differ in terms of FA elongation. We also searched for some specific products of FA elongation in serum of CRC patients and from healthy volunteers. Tissue and serum FA profiles were determined by means of gas chromatography-mass spectrometry (GC/MS), and the tissue expression of elongases (ELOVLs) was analyzed with real-time PCR. Results: Compared to normal colon tissue, CRC specimens showed significantly higher levels of 22-, 24- and 26-carbon FAs, stronger expressions of ELOVL1 and ELOVL6 (4- and 9-fold elevated respectively), and higher values of 18: 0/16: 0 elongation index. We also demonstrated presence of cerotic acid (26: 0) in serum of all CRC patients but in none of the healthy controls. Conclusions: CRC tissue seems to be characterized by enhanced FA elongation (hyper-elongation). Presence of cerotic acid in CRC patients sera and absence of this FA in healthy subjects points to this compound as a strong candidate for specific metabolic marker of colorectal malignancies.

Sign in / Sign up

Export Citation Format

Share Document