Cytomolecular Identification of Individual Wheat-Wheat Chromosome Arm Associations in Wheat-Rye Hybrids

Abstract Background Polyploidy is centrally important in the evolution and domestication of plants because it leads to major genomic changes, such as altered patterns of gene expression, which are thought to underlie the emergence of new traits. Despite the common occurrence of these globally altered patterns of gene expression in polyploids, the mechanisms involved are not well understood. Results Using a precisely defined framework of highly conserved syntenic genes on hexaploid wheat chromosome 3DL and its progenitor 3 L chromosome arm of diploid Aegilops tauschii, we show that 70% of these gene pairs exhibited proportionately reduced gene expression, in which expression in the hexaploid context of the 3DL genes was ∼40% of the levels observed in diploid Ae tauschii. Several genes showed elevated expression during the later stages of grain development in wheat compared with Ae tauschii. Gene sequence and methylation differences probably accounted for only a few cases of differences in gene expression. In contrast, chromosome-wide patterns of reduced chromatin accessibility of genes in the hexaploid chromosome arm compared with its diploid progenitor were correlated with both reduced gene expression and the imposition of new patterns of gene expression. Conclusions Our pilot-scale analyses show that chromatin compaction may orchestrate reduced gene expression levels in the hexaploid chromosome arm of wheat compared to its diploid progenitor chromosome arm.

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FERTILITY COMPENSATION OF CORNERSTONE MALE STERILITY OF WHEAT BY RYE

Genetics ◽

10.1093/genetics/104.1.181 ◽

1983 ◽

Vol 104 (1) ◽

pp. 181-189

Author(s):

M A Hossain ◽

C J Driscoll

Keyword(s):

Male Sterility ◽

Male Fertility ◽

Wheat Chromosome ◽

Hybrid Wheat ◽

Specific Expression ◽

Entire Chromosome ◽

Chromosome Arm ◽

Fertility Gene

ABSTRACT The genome of rye is known to compensate for the lost male-fertility gene(s) of wheat chromosome arm 4Aα in the Cornerstone male-sterility mutant. A search for the rye chromosome(s) involved in this compensation showed that chromosomes 2R and 4R are responsible. Only the short arms of these two chromosomes are the operative ones. Chromosome arm 4RS compensates in an erratic way, whereas 2RS compensates in a full and consistent way. The entire chromosome 2R compensates less well than the 2RS telocentric which reflects an antifertility factor(s) on 2RL. This may be a specific expression of the 2R genes for poor vigor which are located on only the long arm. 2RS will be a valuable piece of chromatin for the XYZ system of producing hybrid wheat.

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Comparative physical mapping between wheat chromosome arm 2BL and rice chromosome 4

Genetica ◽

10.1007/s10709-010-9528-y ◽

2010 ◽

Vol 138 (11-12) ◽

pp. 1277-1296 ◽

Cited By ~ 4

Author(s):

Tong Geon Lee ◽

Yong Jin Lee ◽

Dae Yeon Kim ◽

Yong Weon Seo

Keyword(s):

Physical Mapping ◽

Rice Chromosome ◽

Wheat Chromosome ◽

Chromosome 4 ◽

Chromosome Arm

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A LINKAGE STUDY INVOLVING TWO WHEAT-RYE TRANSLOCATION CHROMOSOMES

Canadian Journal of Genetics and Cytology ◽

10.1139/g69-018 ◽

1969 ◽

Vol 11 (1) ◽

pp. 143-146 ◽

Cited By ~ 1

Author(s):

C. J. Driscoll ◽

G. D. Patil

Keyword(s):

Genetic Markers ◽

Transmission Rate ◽

Wheat Chromosome ◽

Linkage Study ◽

Linkage Test ◽

Crossing Over ◽

Complete Linkage ◽

Transmission Rates ◽

Chromosome Arm

Two wheat-rye translocation chromosomes involving the same wheat chromosome arm but different rye segments were subjected to a linkage test. Apparently no crossing over occurred between the non-homologous rye segments. The genetic markers possessed by these rye segments showed complete linkage in repulsion. The male transmission rates of the translocation chromosomes agree with those expected on the basis of the male transmission rate of each when competing with the normal wheat chromosome.

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Integrated physical map of bread wheat chromosome arm 7DS to facilitate gene cloning and comparative studies

New Biotechnology ◽

10.1016/j.nbt.2018.03.003 ◽

2019 ◽

Vol 48 ◽

pp. 12-19 ◽

Cited By ~ 5

Author(s):

Zuzana Tulpová ◽

Ming-Cheng Luo ◽

Helena Toegelová ◽

Paul Visendi ◽

Satomi Hayashi ◽

...

Keyword(s):

Gene Cloning ◽

Bread Wheat ◽

Comparative Studies ◽

Physical Map ◽

Wheat Chromosome ◽

Chromosome Arm

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High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harbouring the Fusarium head blight resistance QTLQfhs.ifa-5A

Plant Biotechnology Journal ◽

10.1111/pbi.12850 ◽

2017 ◽

Vol 16 (5) ◽

pp. 1046-1056 ◽

Cited By ~ 20

Author(s):

Maria Buerstmayr ◽

Barbara Steiner ◽

Christian Wagner ◽

Petra Schwarz ◽

Klaus Brugger ◽

...

Keyword(s):

High Resolution ◽

Fusarium Head Blight ◽

Fusarium Head Blight Resistance ◽

Wheat Chromosome ◽

Pericentromeric Region ◽

Blight Resistance ◽

Head Blight ◽

High Resolution Mapping ◽

Chromosome Arm ◽

Resolution Mapping

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Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture

Genome ◽

10.1139/g00-020 ◽

2000 ◽

Vol 43 (5) ◽

pp. 756-762 ◽

Cited By ~ 14

Author(s):

Hong-Jie Li ◽

Bei-Hai Guo ◽

Yi-Wen Li ◽

Li-Qun Du ◽

Xu Jia ◽

...

Keyword(s):

Triticum Aestivum ◽

Tissue Culture ◽

In Situ Hybridization ◽

Wheat Chromosome ◽

Triticum Aestivum L ◽

Average Frequency ◽

Dasypyrum Villosum ◽

Chromosome Translocations ◽

Chromosome Arm

Fluorescence in situ hybridization (FISH) was applied with total genomic DNA extracted from Dasypyrum villosum (L.) Candargy as a probe to characterize chromosome translocations arising from tissue culture in hybrids of Triticum aestivum × (T. durum - D. villosum, amphiploid). Chromosome translocations between wheat and D. villosum occurred in callus cells at an average frequency of 1.9%. Translocations existed not only in callus cells but also in regenerants. Three plants with translocation chromosomes were characterized among 66 regenerants of T. aestivum 'Chinese Spring' × 'TH1W' and 'NPFP' × 'TH1'. One of them proved to be a reciprocal translocation with an exchange of about one third of a wheat chromosome arm with about one half of a chromosome arm of D. villosum. The breakpoints of the other two translocations were located at, or near centromeres. The results are similar for both callus cells and regenerants and provide further evidence that translocations take place in tissue culture. Other structural chromosomal changes, for example, fragments, telocentrics, dicentromeres, and deletions, as well as numerical alterations including aneuploidy and polyploidy were recorded both in callus cells and regenerants.Key words: wheat, Dasypyrum villosum, translocation, genomic in situ hybridization, tissue culture.

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