Human Dental Pulp Stem Cells Differentiate into Oligodendrocyte Progenitors Using the Expression of Olig2 Transcription Factor

Msh homeobox 1 (MSX1) encodes a transcription factor implicated in embryonic development of limbs and craniofacial tissues including bone and teeth. Although MSX1 regulates osteoblast differentiation in the cranial bone of young animal, little is known about the contribution of MSX1 to the osteogenic potential of human cells. In the present study, we investigate the role of MSX1 in osteogenic differentiation of human dental pulp stem cells isolated from deciduous teeth. When these cells were exposed to osteogenesis-induction medium, runt-related transcription factor-2(RUNX2), bone morphogenetic protein-2(BMP2), alkaline phosphatase(ALPL), and osteocalcin(OCN)mRNA levels, as well as alkaline phosphatase activity, increased on days 4–12, and thereafter the matrix was calcified on day 14. However, knockdown ofMSX1with small interfering RNA abolished the induction of the osteoblast-related gene expression, alkaline phosphatase activity, and calcification. Interestingly, DNA microarray and PCR analyses revealed thatMSX1knockdown induced the sterol regulatory element-binding protein 2(SREBP2)transcriptional factor and its downstream target genes in the cholesterol synthesis pathway. Inhibition of cholesterol synthesis enhances osteoblast differentiation of various mesenchymal cells. Thus, MSX1 may downregulate the cholesterol synthesis-related genes to ensure osteoblast differentiation of human dental pulp stem cells.

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Modulation of Cell Death and Promotion of Chondrogenic Differentiation by Fas/FasL in Human Dental Pulp Stem Cells (hDPSCs)

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2020.00279 ◽

2020 ◽

Vol 8 ◽

Cited By ~ 1

Author(s):

Alessandra Pisciotta ◽

Giulia Bertani ◽

Laura Bertoni ◽

Rosanna Di Tinco ◽

Sara De Biasi ◽

...

Keyword(s):

Stem Cells ◽

Cell Death ◽

Dental Pulp ◽

Chondrogenic Differentiation ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Chrysin induces osteogenic differentiation of human dental pulp stem cells

Experimental Cell Research ◽

10.1016/j.yexcr.2020.112466 ◽

2021 ◽

Vol 400 (2) ◽

pp. 112466

Author(s):

J.F. Huo ◽

M.L. Zhang ◽

X.X. Wang ◽

D.H. Zou

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp

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Human Dental Pulp Stem Cells (DPSCs) Therapy in Rescuing Photoreceptors and Establishing a Sodium Iodate-Induced Retinal Degeneration Rat Model

Tissue Engineering and Regenerative Medicine ◽

10.1007/s13770-020-00312-1 ◽

2021 ◽

Author(s):

Chenshen Lam ◽

Hiba Amer Alsaeedi ◽

Avin Ee-Hwan Koh ◽

Mohd Hairul Nizam Harun ◽

Angela Ng Min Hwei ◽

...

Keyword(s):

Stem Cells ◽

Retinal Degeneration ◽

Rat Model ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Sodium Iodate ◽

Human Dental Pulp

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Methylglyoxal-Dependent Glycative Stress Is Prevented by the Natural Antioxidant Oleuropein in Human Dental Pulp Stem Cells through Nrf2/Glo1 Pathway

Antioxidants ◽

10.3390/antiox10050716 ◽

2021 ◽

Vol 10 (5) ◽

pp. 716

Author(s):

Simona Delle Delle Monache ◽

Fanny Pulcini ◽

Roberta Frosini ◽

Vincenzo Mattei ◽

Vincenzo Nicola Talesa ◽

...

Keyword(s):

Stem Cells ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

Great Promise ◽

Oral Diseases ◽

Bioactive Component ◽

Glycation End Products ◽

Abnormal Accumulation ◽

Synthetic Agents ◽

Human Dental Pulp

Methylglyoxal (MG) is a potent precursor of glycative stress (abnormal accumulation of advanced glycation end products, AGEs), a relevant condition underpinning the etiology of several diseases, including those of the oral cave. At present, synthetic agents able to trap MG are known; however, they have never been approved for clinical use because of their severe side effects. Hence, the search of bioactive natural scavengers remains a sector of strong research interest. Here, we investigated whether and how oleuropein (OP), the major bioactive component of olive leaf, was able to prevent MG-dependent glycative stress in human dental pulp stem cells (DPSCs). The cells were exposed to OP at 50 µM for 24 h prior to the administration of MG at 300 µM for additional 24 h. We found that OP prevented MG-induced glycative stress and DPSCs impairment by restoring the activity of Glyoxalase 1 (Glo1), the major detoxifying enzyme of MG, in a mechanism involving the redox-sensitive transcription factor Nrf2. Our results suggest that OP holds great promise for the development of preventive strategies for MG-derived AGEs-associated oral diseases and open new paths in research concerning additional studies on the protective potential of this secoiridoid.

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