Calcium Phosphate-Coated Titanium Implants in the Mandible: Limitations of the in vivo Minipig Model

2020 ◽  
Vol 61 (6) ◽  
pp. 177-187
Author(s):  
Till Kämmerer ◽  
Tony Lesmeister ◽  
Victor Palarie ◽  
Eik Schiegnitz ◽  
Andrea Schröter ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Statistical Difference ◽  
Surface Modifications ◽  
Titanium Implants ◽  
In Vivo Model ◽  
Implant Surface ◽  
Press Fit ◽  
Endosseous Implant ◽  
Bone To Implant Contact

Introduction: We aimed to compare implant osseointegration with calcium phosphate (CaP) surfaces and rough subtractive-treated sandblasted/acid etched surfaces (SA) in an in vivo minipig mandible model. Materials and Methods: A total of 36 cylindrical press-fit implants with two different surfaces (CaP, n = 18; SA, n = 18) were inserted bilaterally into the mandible of 9 adult female minipigs. After 2, 4, and 8 weeks, we analyzed the cortical bone-to-implant contact (cBIC; %) and area coverage of bone-to-implant contact within representative bone chambers (aBIC; %). Results: After 2 weeks, CaP implants showed no significant increase in cBIC and aBIC compared to SA (cBIC: mean 38 ± 5 vs. 16 ± 11%; aBIC: mean 21 ± 1 vs. 6 ± 9%). Two CaP implants failed to achieve osseointegration. After 4 weeks, no statistical difference between CaP and SA was seen for cBIC (mean 54 ± 15 vs. 43 ± 16%) and aBIC (mean 43 ± 28 vs. 32 ± 6). However, we excluded two implants in each group due to failure of osseointegration. After 8 weeks, we observed no significant intergroup differences (cBIC: 18 ± 9 vs. 18 ± 20%; aBIC: 13 ± 8 vs. 16 ± 9%). Again, three CaP implants and two SA implants had to be excluded due to failure of osseointegration. Conclusion: Due to multiple implant losses, we cannot recommend the oral mandibular minipig in vivo model for future endosseous implant research. Considering the higher rate of osseointegration failure, CaP coatings may provide an alternative to common subtractive implant surface modifications in the early phase post-insertion.

scholarly journals Multi-Omics Analysis of Oral Bacterial Biofilm on Titanium Oxide Nanostructure Modified Implant Surface: in Vivo Sequencing-Based Pilot Study in Beagle Dogs

2021 ◽  
Author(s):  
Hanyu Sun ◽  
Yuki Chan ◽  
Xuan Li ◽  
Ruogu Xu ◽  
Zhengchuan Zhang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Antimicrobial Properties ◽  
Surface Modifications ◽  
Titanium Implants ◽  
Bacterial Biofilm ◽  
Bacterial Invasion ◽  
Oral Microbiota ◽  
Minimal Impact ◽  
The Impact

Abstract Background and aimsSurface modifications of titanium implants play essential role in facilitating osteointegration and enhancing their antimicrobial properties, while the latter is critical for preventing infectious diseases caused by the biofilm. However, it remains unknown about how the surface modifications could affect the composition and functional gene expression of oral microbiota deposited on the titanium implants. In this study, we aimed to investigate the impact of different nanostructured surfaces on the biofilm in vivo.ResultsNanophase calcium phosphate were successfully deposited into or between the TiO2 nanotubes with a diameter of 70–90 nm. NT and NTN surfaces showed increased roughness than the MP surface. XPS spectra showed that the O 1s was mainly divided into two bands in MP and NT samples, including Ti-O and -OH, while the surface modification of TiO2 nanotube in NT accounted for the increased intensity of Ti-O with the reference to that in MP samples. After the deposition of calcium phosphate, two new elemental peaks of Ca and P can be identified from the XPS survey spectrum of NTN. Moreover, the O 1s of NTN sample could be differentiated into three peaks, while the new one represented the -PO band. The 16S rDNA sequencing results showed that NT and NTN had minimal impact on the diversity and community structure of oral microbiota. Metatranscriptomic sequencing revealed that differentially expressed genes (DEGs) mostly differed in the terms of the biological process and cellular component on different surfaces. Gene Ontology (GO) terms enrichment indicated that NTN down-regulate the genes associated in localization and locomotion. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the DEGs were associated with microbial metabolism, protein synthesis and bacterial invasion of epithelial cells.ConclusionTiO2 nanotube and calcium phosphate-coated TiO2 nanotube despite improving the antimicrobial properties of implant surfaces, had unexpectedly minimal impact on the microbiome composition and diversity. Notably, nanostructured titanium surfaces could inhibit the bacterial migration and colonization, down-regulate the pathogen invasion pathways, and further destruct bacterial cellular membrane, all in all, conferred the bactericidal properties.

Preservation of Bone Tissue Quality during the Usage of Synthetic Bioactive Calcium Phosphate Mineral Coating for Prevention of Metallic Construction Migration

2020 ◽  
Vol 46 ◽  
pp. 67-74
Author(s):  
Alexander Markov ◽  
Andrey Ponomarev ◽  
Mikhail Zavatskij ◽  
Ksenya Stepanova ◽  
Sergej Krylov ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Tissue ◽  
Titanium Implants ◽  
Mineral Density ◽  
Tissue Quality ◽  
Implant Surface ◽  
X Ray ◽  
Phosphate Mineral ◽  
Negative Effect

This work is devoted to studying the reaction of bone tissue to installed titanium implants, which are bioinert in comparison with other metals used in traumatological and orthopedic practice, but which have a negative effect on bone tissue in the implant-to-bone area. In order to increase the affinity of the implant surface with bone tissue, it was proposed to use synthetic bioactive calcium phosphate mineral coating by applying it on titanium implants, which ultimately will make it possible to maintain the quality of the bone tissue around the implant after osteosynthesis or prosthetics. During the comparative research an in vivo experiment was conducted in which quality change of peri-implant zone of bone tissue when using titanium implants with synthetic bioactive calcium phosphate mineral coating and titanium implants without bioactive coating was analyzed. To analyze the results of the study, we used the X-ray computed tomography method, for a detailed assessment of the X-ray density and bone mineral density of the peri-implant zone. According to the results of the experiment, it was concluded that the reaction of bone tissue to synthetic bioactive calcium phosphate mineral coating of titanium implants was reduced, what ensures the preservation of bone tissue quality in the postoperative period at a high level.

Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

2009 ◽  
Vol 631-632 ◽  
pp. 211-216 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
T. Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

scholarly journals Calcium Phosphate Ceramics Can Prevent Bisphosphonate-Related Osteonecrosis of the Jaw

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1955
Author(s):  
Siri Paulo ◽  
Mafalda Laranjo ◽  
Anabela Paula ◽  
Ana Margarida Abrantes ◽  
João Martins ◽  
...  
Keyword(s):  
Nuclear Medicine ◽  
Calcium Phosphate ◽  
Tooth Extraction ◽  
Soft Tissues ◽  
Calcium Phosphates ◽  
Test Group ◽  
Osteonecrosis Of The Jaw ◽  
In Vivo Model ◽  
Calcium Phosphate Ceramics

Bisphosphonate-associated osteonecrosis of the jaw (BRONJ), a post-surgical non-healing wound condition, is one of the most common side effects in patients treated with nitrogen-containing bisphosphonates. Its physiopathology has been related with suppression of bone turnover, of soft tissue healing and infection. Biphasic calcium phosphates (BCP) are used as a drug delivery vehicle and as a bone substitute in surgical wounds. Due to their capacity to adsorb zoledronate, it was hypothesized these compounds might have a protective effect on the soft tissues in BRONJ wounds. To address this hypothesis, a reproducible in vivo model of BRONJ in Wistar rats was used. This model directly relates chronic bisphosphonate administration with the development of osteonecrosis of the jaw after tooth extraction. BCP granules were placed in the alveolus immediately after tooth extraction in the test group. The animals were evaluated through nuclear medicine, radiology, macroscopic observation, and histologic analysis. Encouragingly, calcium phosphate ceramics were able to limit zoledronate toxicity in vivo and to favor healing, which was evidenced by medical imaging (nuclear medicine and radiology), macroscopically, and through histology. The studied therapeutic option presented itself as a potential solution to prevent the development of maxillary osteonecrosis.

scholarly journals Intraosseous Temperature Change during Installation of Dental Implants with Two Different Surfaces and Different Drilling Protocols: An In Vivo Study in Sheep

2019 ◽  
Vol 8 (8) ◽  
pp. 1198 ◽  
Author(s):  
Michele Stocchero ◽  
Yohei Jinno ◽  
Marco Toia ◽  
Marianne Ahmad ◽  
Evaggelia Papia ◽  
...  
Keyword(s):  
Temperature Increase ◽  
Element Model ◽  
Multiple Regression Model ◽  
Metatarsal Bone ◽  
Area Fraction ◽  
Bone Area ◽  
Implant Surface ◽  
Bone Necrosis ◽  
Bone To Implant Contact

Background: The intraosseous temperature during implant installation has never been evaluated in an in vivo controlled setup. The aims were to investigate the influence of a drilling protocol and implant surface on the intraosseous temperature during implant installation, to evaluate the influence of temperature increase on osseointegration and to calculate the heat distribution in cortical bone. Methods: Forty Brånemark implants were installed into the metatarsal bone of Finnish Dorset crossbred sheep according to two different drilling protocols (undersized/non-undersized) and two surfaces (moderately rough/turned). The intraosseous temperature was recorded, and Finite Element Model (FEM) was generated to understand the thermal behavior. Non-decalcified histology was carried out after five weeks of healing. The following osseointegration parameters were calculated: Bone-to-implant contact (BIC), Bone Area Fraction Occupancy (BAFO), and Bone Area Fraction Occupancy up to 1.5 mm (BA1.5). A multiple regression model was used to identify the influencing variables on the histomorphometric parameters. Results: The temperature was affected by the drilling protocol, while no influence was demonstrated by the implant surface. BIC was positively influenced by the undersized drilling protocol and rough surface, BAFO was negatively influenced by the temperature rise, and BA1.5 was negatively influenced by the undersized drilling protocol. FEM showed that the temperature at the implant interface might exceed the limit for bone necrosis. Conclusion: The intraosseous temperature is greatly increased by an undersized drilling protocol but not from the implant surface. The temperature increase negatively affects the bone healing in the proximity of the implant. The undersized drilling protocol for Brånemark implant systems increases the amount of bone at the interface, but it negatively impacts the bone far from the implant.

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

Nanoscale ◽  
2015 ◽  
Vol 7 (21) ◽  
pp. 9908-9918 ◽  
Author(s):  
Elisabeth Rieger ◽  
Agnès Dupret-Bories ◽  
Laetitia Salou ◽  
Marie-Helene Metz-Boutigue ◽  
Pierre Layrolle ◽  
...  
Keyword(s):  
Surface Modification ◽  
Soft Tissue ◽  
Surface Modifications ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Tissue Response ◽  
Soft Tissue Response ◽  
Modification Of Titanium

Nanoscale surface modification of titanium microbeads can control the soft tissue response in vitro and in vivo.

scholarly journals The Effect of Hydroxyapatite Nanocrystals on Osseointegration of Titanium Implants: AnIn VivoRabbit Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Karin Breding ◽  
Ryo Jimbo ◽  
Mariko Hayashi ◽  
Ying Xue ◽  
Kamal Mustafa ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Calcium Phosphates ◽  
Surface Characteristics ◽  
Healing Time ◽  
Titanium Implants ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Implant Surface ◽  
Hydroxyapatite Nanocrystals

Osseointegration is dependent on implant surface characteristics, including surface chemistry and topography. The presence of nanosized calcium phosphates on the implant surface is interesting to investigate since they affect both the nanotopography and surface chemistry, forming a bone mineral resembling surface. In this work, the osseointegration of titanium implants with and without the presence of hydroxyapatite (HA) nanocrystals has been evaluatedin vivo. The integration was examined using removal torque measurements and real-time polymerase chain reaction (RT-PCR) analysis. The study was performed using two healing time points, 3 and 12 weeks. The results showed that the torque needed to remove the implants was insignificant between the non- and HA-coated implants, both at weeks 3 and 12. The RT-PCR, however, showed significant differences for osteoblast, osteoclast, and proinflammation markers when HA nanocrystals were present.

The Effect of Bioceramic Surface Modifications on Different Endosseous Implant and Surgical Design. A Series of In Vivo Studies in Dogs.

2008 ◽  
Vol 396-398 ◽  
pp. 385-388
Author(s):  
Paulo Guilherme Coelho ◽  
Marcelo Suzuki
Keyword(s):  
Close Contact ◽  
In Vivo Studies ◽  
Implant Design ◽  
Implant Surface ◽  
Intimate Contact ◽  
Endosseous Implant ◽  
Level Of Significance ◽  
Post Hoc ◽  
Appositional Bone

The objective of this series of experiments was to evaluate the effect of bioceramic coatings/ incorporations on implant surfaces as a function of implant and surgical drilling design. Methods: A series of four in vivo studies were conducted utilizing the dog proximal tibia model. The models provided implants that remained from 2 to 5 weeks implantation time. The different studies comprised the placement of implants with intimate contact with bone following placement and implant designs that resulted in healing chambers. The various implant types presented surfaces with and without Ca- and P-based bioceramic incorporations. Biomechanical and histomorphometric measurements along with qualitative bone-implant interface morphology evaluation were performed. For all studies, one-way ANOVA at 95% level of significance was employed along with Tukey's post-hoc multiple comparisons. Results: Close contact between cortical and trabecular bone and all the different implant surfaces irrespective of implant fit (with and without healing chambers) showed that all surfaces were biocompatible and osteoconductive. In general, appositional bone healing was observed at all implant regions that were in intimate contact with bone immediately after placement, and an intramembranous-like healing occurred throughout the whole volume of the healing chambers. Irrespective of implant + surgical drilling design, the presence of Ca and P resulted in a bone morphology that showed primary osteonic structures at earlier times than uncoated surfaces. Conclusion: Irrespective of implant design and surgical drilling combination, the presence of Ca and P on the implant surface positively modulated early healing around endosseous implants.

Histologic Evaluation of a Provisional Implant Retrieved From Man 7 Months After Placement in a Sinus Augmented With Calcium Sulphate: A Case Report

2007 ◽  
Vol 33 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Giovanna Iezzi ◽  
Elisabetta Fiera ◽  
Antonio Scarano ◽  
Gabriele Pecora ◽  
Adriano Piattelli
Keyword(s):  
Close Contact ◽  
Calcium Sulphate ◽  
Titanium Implants ◽  
Implant Surface ◽  
Sinus Augmentation ◽  
Bone Implant ◽  
Native Bone ◽  
Osteocyte Lacunae ◽  
Histologic Evaluation

Abstract Little is known about the in vivo healing processes at the interface of implants placed in different grafting materials. For optimal sinus augmentation, a bone graft substitute that can regenerate high-quality bone and enable the osseointegration of load-bearing titanium implants is needed in clinical practice. Calcium sulphate (CaS) is one of the oldest biomaterials used in medicine, but few studies have addressed its use as a sinus augmentation material in conjunction with simultaneous implant placement. The aim of the present study was to histologically evaluate an immediately loaded provisional implant retrieved 7 months after simultaneous placement in a human sinus grafted with CaS. During retrieval bone detached partially from one of the implants which precluded its use for histologic analysis. The second implant was completely surrounded by native and newly formed bone, and it underwent histologic evaluation. Lamellar bone, with small osteocyte lacunae, was present and in contact with the implant surface. No gaps, epithelial cells, or connective tissues were present at the bone–implant interface. No residual CaS was present. Bone–implant contact percentage was 55% ± 8%. Of this percentage, 40% was represented by native bone and 15% by newly formed bone. CaS showed complete resorption and new bone formation in the maxillary sinus; this bone was found to be in close contact with the implant surface after immediate loading.

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