scholarly journals Multi-Omics Analysis of Oral Bacterial Biofilm on Titanium Oxide Nanostructure Modified Implant Surface: in Vivo Sequencing-Based Pilot Study in Beagle Dogs

2021 ◽  
Author(s):  
Hanyu Sun ◽  
Yuki Chan ◽  
Xuan Li ◽  
Ruogu Xu ◽  
Zhengchuan Zhang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Antimicrobial Properties ◽  
Surface Modifications ◽  
Titanium Implants ◽  
Bacterial Biofilm ◽  
Bacterial Invasion ◽  
Oral Microbiota ◽  
Minimal Impact ◽  
The Impact

Abstract Background and aimsSurface modifications of titanium implants play essential role in facilitating osteointegration and enhancing their antimicrobial properties, while the latter is critical for preventing infectious diseases caused by the biofilm. However, it remains unknown about how the surface modifications could affect the composition and functional gene expression of oral microbiota deposited on the titanium implants. In this study, we aimed to investigate the impact of different nanostructured surfaces on the biofilm in vivo.ResultsNanophase calcium phosphate were successfully deposited into or between the TiO2 nanotubes with a diameter of 70–90 nm. NT and NTN surfaces showed increased roughness than the MP surface. XPS spectra showed that the O 1s was mainly divided into two bands in MP and NT samples, including Ti-O and -OH, while the surface modification of TiO2 nanotube in NT accounted for the increased intensity of Ti-O with the reference to that in MP samples. After the deposition of calcium phosphate, two new elemental peaks of Ca and P can be identified from the XPS survey spectrum of NTN. Moreover, the O 1s of NTN sample could be differentiated into three peaks, while the new one represented the -PO band. The 16S rDNA sequencing results showed that NT and NTN had minimal impact on the diversity and community structure of oral microbiota. Metatranscriptomic sequencing revealed that differentially expressed genes (DEGs) mostly differed in the terms of the biological process and cellular component on different surfaces. Gene Ontology (GO) terms enrichment indicated that NTN down-regulate the genes associated in localization and locomotion. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the DEGs were associated with microbial metabolism, protein synthesis and bacterial invasion of epithelial cells.ConclusionTiO2 nanotube and calcium phosphate-coated TiO2 nanotube despite improving the antimicrobial properties of implant surfaces, had unexpectedly minimal impact on the microbiome composition and diversity. Notably, nanostructured titanium surfaces could inhibit the bacterial migration and colonization, down-regulate the pathogen invasion pathways, and further destruct bacterial cellular membrane, all in all, conferred the bactericidal properties.

Calcium Phosphate-Coated Titanium Implants in the Mandible: Limitations of the in vivo Minipig Model

2020 ◽  
Vol 61 (6) ◽  
pp. 177-187
Author(s):  
Till Kämmerer ◽  
Tony Lesmeister ◽  
Victor Palarie ◽  
Eik Schiegnitz ◽  
Andrea Schröter ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Statistical Difference ◽  
Surface Modifications ◽  
Titanium Implants ◽  
In Vivo Model ◽  
Implant Surface ◽  
Press Fit ◽  
Endosseous Implant ◽  
Bone To Implant Contact

Introduction: We aimed to compare implant osseointegration with calcium phosphate (CaP) surfaces and rough subtractive-treated sandblasted/acid etched surfaces (SA) in an in vivo minipig mandible model. Materials and Methods: A total of 36 cylindrical press-fit implants with two different surfaces (CaP, n = 18; SA, n = 18) were inserted bilaterally into the mandible of 9 adult female minipigs. After 2, 4, and 8 weeks, we analyzed the cortical bone-to-implant contact (cBIC; %) and area coverage of bone-to-implant contact within representative bone chambers (aBIC; %). Results: After 2 weeks, CaP implants showed no significant increase in cBIC and aBIC compared to SA (cBIC: mean 38 ± 5 vs. 16 ± 11%; aBIC: mean 21 ± 1 vs. 6 ± 9%). Two CaP implants failed to achieve osseointegration. After 4 weeks, no statistical difference between CaP and SA was seen for cBIC (mean 54 ± 15 vs. 43 ± 16%) and aBIC (mean 43 ± 28 vs. 32 ± 6). However, we excluded two implants in each group due to failure of osseointegration. After 8 weeks, we observed no significant intergroup differences (cBIC: 18 ± 9 vs. 18 ± 20%; aBIC: 13 ± 8 vs. 16 ± 9%). Again, three CaP implants and two SA implants had to be excluded due to failure of osseointegration. Conclusion: Due to multiple implant losses, we cannot recommend the oral mandibular minipig in vivo model for future endosseous implant research. Considering the higher rate of osseointegration failure, CaP coatings may provide an alternative to common subtractive implant surface modifications in the early phase post-insertion.

scholarly journals Impact of surface topography and coating on osteogenesis and bacterial attachment on titanium implants

2018 ◽  
Vol 9 ◽  
pp. 204173141879069 ◽  
Author(s):  
Laila Damiati ◽  
Marcus G Eales ◽  
Angela H Nobbs ◽  
Bo Su ◽  
Penelope M Tsimbouri ◽  
...  
Keyword(s):  
Surface Topography ◽  
Dental Implants ◽  
Antimicrobial Properties ◽  
Bacterial Attachment ◽  
Titanium Implants ◽  
Premature Failure ◽  
Biological Environment ◽  
The Impact

Titanium (Ti) plays a predominant role as the material of choice in orthopaedic and dental implants. Despite the majority of Ti implants having long-term success, premature failure due to unsuccessful osseointegration leading to aseptic loosening is still too common. Recently, surface topography modification and biological/non-biological coatings have been integrated into orthopaedic/dental implants in order to mimic the surrounding biological environment as well as reduce the inflammation/infection that may occur. In this review, we summarize the impact of various Ti coatings on cell behaviour both in vivo and in vitro. First, we focus on the Ti surface properties and their effects on osteogenesis and then on bacterial adhesion and viability. We conclude from the current literature that surface modification of Ti implants can be generated that offer both osteoinductive and antimicrobial properties.

Calcium Phosphate Coating on Blast-Treated Titanium Implants by RF Magnetron Sputtering

2009 ◽  
Vol 631-632 ◽  
pp. 211-216 ◽  
Author(s):  
Kyosuke Ueda ◽  
Takayuki Narushima ◽  
Takashi Goto ◽  
T. Katsube ◽  
Hironobu Nakagawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Magnetron Sputtering ◽  
Bonding Strength ◽  
Rf Magnetron Sputtering ◽  
Titanium Implants ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating ◽  
Coating Films

Calcium phosphate coating films were fabricated on Ti-6Al-4V plates and screw-type implants with a blast-treated surface using radiofrequency (RF) magnetron sputtering and were evaluated in vitro and in vivo. Amorphous calcium phosphate (ACP) and oxyapatite (OAp) films obtained in this study could cover the blast-treated substrate very efficiently, maintaining the surface roughness. For the in vitro evaluations of the calcium phosphate coating films, bonding strength and alkaline phosphatase (ALP) activity were examined. The bonding strength of the coating films to a blast-treated substrate exceeded 60 MPa, independent of film phases except for the film after post-heat-treatment in silica ampoule. When compared with an uncoated substrate, the increase in the ALP activity of osteoblastic SaOS-2 cells on a calcium phosphate coated substrate was confirmed by a cell culture test. The removal torque of screw-type Ti-6Al-4V implants with a blast-treated surface from the femur of Japanese white rabbit increased with the duration of implantation and it was statistically improved by coating an ACP film 2 weeks after implantation. The in vitro and in vivo studies suggested that the application of the sputtered ACP film as a coating on titanium implants was effective in improving their biocompatibility with bones.

scholarly journals Biocompatibility and Bone Formation of Flexible, Cotton Wool-like PLGA/Calcium Phosphate Nanocomposites in Sheep

2011 ◽  
Vol 5 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Oliver D Schneider ◽  
Dirk Mohn ◽  
Roland Fuhrer ◽  
Karina Klein ◽  
Käthi Kämpf ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Antimicrobial Properties ◽  
Drill Hole ◽  
Bone Defects ◽  
Long Bone ◽  
Minimal Amount ◽  
Cotton Wool

Background: The purpose of this preliminary study was to assess the in vivo performance of synthetic, cotton wool-like nanocomposites consisting of a biodegradable poly(lactide-co-glycolide) fibrous matrix and containing either calcium phosphate nanoparticles (PLGA/CaP 60:40) or silver doped CaP nanoparticles (PLGA/Ag-CaP 60:40). Besides its extraordinary in vitro bioactivity the latter biomaterial (0.4 wt% total silver concentration) provides additional antimicrobial properties for treating bone defects exposed to microorganisms. Materials and Methods: Both flexible artificial bone substitutes were implanted into totally 16 epiphyseal and metaphyseal drill hole defects of long bone in sheep and followed for 8 weeks. Histological and histomorphological analyses were conducted to evaluate the biocompatibility and bone formation applying a score system. The influence of silver on the in vivo performance was further investigated. Results: Semi-quantitative evaluation of histology sections showed for both implant materials an excellent biocompatibility and bone healing with no resorption in the adjacent bone. No signs of inflammation were detectable, either macroscopically or microscopically, as was evident in 5 µm plastic sections by the minimal amount of inflammatory cells. The fibrous biomaterials enabled bone formation directly in the centre of the former defect. The area fraction of new bone formation as determined histomorphometrically after 8 weeks implantation was very similar with 20.5 ± 11.2 % and 22.5 ± 9.2 % for PLGA/CaP and PLGA/Ag-CaP, respectively. Conclusions: The cotton wool-like bone substitute material is easily applicable, biocompatible and might be beneficial in minimal invasive surgery for treating bone defects.

Partially desulfated heparin modulates the interaction between anti-protamine/heparin antibodies and platelets

2016 ◽  
Vol 115 (02) ◽  
pp. 324-332 ◽  
Author(s):  
Rabie Jouni ◽  
Heike Zöllner ◽  
Ahmad Khadour ◽  
Jan Wesche ◽  
Anne Grotevendt ◽  
...  
Keyword(s):  
Platelet Activation ◽  
Platelet Factor ◽  
Standard Drug ◽  
Platelet Surface ◽  
Minimal Impact ◽  
Platelet Survival ◽  
Heparin Complexes ◽  
The Impact

SummaryProtamine (PRT) is the standard drug to neutralise heparin. PRT/heparin complexes induce an immune response similar to that observed in heparin-induced thrombocytopenia (HIT). Partially desulfated heparin (ODSH) was shown to interfere with anti-platelet factor 4/heparin antibodies (Abs), which are responsible for HIT. In this study, we analyse the impact of ODSH on the interaction between anti-PRT/heparin Abs and platelets. The ability of ODSH to prevent anti-PRT/heparin Ab-induced platelet destruction in vivo was investigated using the NOD/ SCID mouse model. ODSH improved platelet survival in the presence of PRT, heparin and anti-PRT/heparin Abs (median platelet survival after 300 minutes (min) with 20 μg/ml ODSH: 75 %, range 70–81 % vs without ODSH: 49%, range 44–59%, p=0.006). Furthermore, when ODSH was applied 60 min after Ab injection platelet survival was improved (median platelet survival after 300 min with ODSH: 83 %, range 77–93 % vs without ODSH: 59 %, range 29–61 %, p=0.02). In in vitro experiments ODSH inhibited platelet activation at concentrations > 16 μg/mL (p< 0.001), as well as PRT/heparin complex binding to platelets (mean fluorescence intensity [MFI] without ODSH: 85 ± 14 vs with ODSH: 15 ± 0.6, p=0.013). ODSH also displaced pre-bound complexes from the platelet surface (MFI without ODSH: 324 ± 43 vs with 32 μg/ml ODSH: 53 ± 9, p< 0.001). While interfering with platelet activation by anti-PRT/heparin Abs, up to a concentration of 16 μg/ml, ODSH had only minimal impact on neutralisation of heparin by PRT. In conclusion, our study shows that ODSH is able to inhibit platelet activation and destruction suggesting a potential clinical use to reduce anti-PRT/heparin Ab-mediated adverse effects.

scholarly journals Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

1998 ◽  
Vol 42 (4) ◽  
pp. 895-898 ◽  
Author(s):  
Silvia Schwank ◽  
Zarko Rajacic ◽  
Werner Zimmerli ◽  
Jürg Blaser
Keyword(s):  
Animal Model ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Phenotypic Resistance ◽  
Vitro Model ◽  
The Impact ◽  
Isogenic Strains

ABSTRACT The impact of bacterial adherence on antibiotic activity was analyzed with two isogenic strains of Staphylococcus epidermidis that differ in the features of their in vitro biofilm formation. The eradication of bacteria adhering to glass beads by amikacin, levofloxacin, rifampin, or teicoplanin was studied in an animal model and in a pharmacokinetically matched in vitro model. The features of S. epidermidis RP62A that allowed it to grow on surfaces in multiple layers promoted phenotypic resistance to antibiotic treatment, whereas strain M7 failed to accumulate, despite initial adherence on surfaces and growth in suspension similar to those for RP62A. Biofilms of S. epidermidis M7 were better eradicated than those of strain RP62A in vitro (46 versus 31%;P < 0.05) as well as in the animal model (39 versus 9%; P < 0.01).

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

Nanoscale ◽  
2015 ◽  
Vol 7 (21) ◽  
pp. 9908-9918 ◽  
Author(s):  
Elisabeth Rieger ◽  
Agnès Dupret-Bories ◽  
Laetitia Salou ◽  
Marie-Helene Metz-Boutigue ◽  
Pierre Layrolle ◽  
...  
Keyword(s):  
Surface Modification ◽  
Soft Tissue ◽  
Surface Modifications ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Tissue Response ◽  
Soft Tissue Response ◽  
Modification Of Titanium

Nanoscale surface modification of titanium microbeads can control the soft tissue response in vitro and in vivo.

scholarly journals In-Vitro Evaluation of Biological Activity of New Series of 1, 3, 4-Oxadiazole Derivatives Against Bap Gene Expression in Acinetobacter bumanni Biofilm

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sedigheh Akbarnezhad Ghareh Lar ◽  
Nakisa Zarrabi Ahrabi ◽  
Yasin SarveAhrabi
Keyword(s):  
Biofilm Formation ◽  
Functional Group ◽  
Biological Activities ◽  
Antimicrobial Properties ◽  
Housekeeping Gene ◽  
Bacterial Biofilm ◽  
Wide Range ◽  
Oxadiazole Derivatives ◽  
The Impact

Background: Acinetobacter bumanni is one of the most common opportunistic pathogens in health centers that is resistant to many antibiotics due to biofilm production. 1, 3, 4-oxadiazoles have a wide range of biological activities. Objectives: The aim of this research was to examine the impact of new 1, 3, 4-oxadiazole derivatives on the expression of biofilm-associated surface protein (Bap), playing an important role in promoting the biofilm formation ability of A. baumannii strains. Methods: Derivatives of 1, 3, 4-oxadiazole were synthesized through a one-step synthesis. A. baumannii strains were identified and isolated in the laboratory. The antimicrobial properties of the synthesized materials against the isolated strains were investigated. DNA, RNA, and cDNA were extracted, and the relative expression of BAP gene in A. baumannii isolates was evaluated by real-time polymerase chain reaction. Results: The compound with methoxyphenyl functional group with MIC = 62.50 mg/mL had the best inhibitory performance among all derivatives. Also, the combination of 4i reduced the expression of the Bap gene by about 24 times, but it had no effect on the expression of the 16srRNA housekeeping gene. Conclusions: 1, 3, 4-oxadiazole derivatives, especially the methoxyphenyl functional group, act as an inhibitor of bacterial biofilm formation and have the potential to be used in the pharmaceutical and biological industries.

Influence of silver-containing filler on antibacterial properties of experimental resin composites against Enterococcus faecalis

2021 ◽  
Vol 109 (2) ◽  
pp. 59-67
Author(s):  
Sanadra Adeeb ◽  
Saja Adeeb ◽  
G. Chladek ◽  
W. Pakieła ◽  
A. Mertas
Keyword(s):  
Enterococcus Faecalis ◽  
Test Performance ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Control Material ◽  
In Vivo Test ◽  
Laboratory Test Results ◽  
The Impact

Purpose: The aim of the presented work was to investigate the impact of the S-P introduction into resin-based composites on their effectiveness against Enterococcus faecalis (E. faecalis). Design/methodology/approach: Seven experimental composites based on typical matrix were developed. Six of them contained a filler with antimicrobial properties (silver sodium hydrogen zirconium phosphate, S-P), while the control material contained only common reinforcement fillers. The materials were characterized in terms of the dispersion of the extender in the matrix and then subjected to microbiological tests. The efficiency in the reduction of E. faecalis in the microenvironment was tested. Findings: The composites show a satisfactory distribution of fillers and a high initial reduction of bacteria colonies for the tested strain of E. faecalis. The reduction in bacteria colonies achieved for S-P concentrations from 7% to 13% was similar (median value from 99.8 to 99.9%, when for control material and compound with 1% S-P the number of colonies increased compared to positive control. Research limitations/implications: Laboratory test results may differ from in vivo test performance. In addition, there are many models for conducting laboratory antimicrobial efficacy studies, the results of which are also varied. The cytotoxic tests, long-term investigations and in vivo experiments need to be performed in future experiments. Practical implications: E. faecalis is a Gram-positive bacterium that is commonly detected in persistent endodontic infections and may enter the root canal through the coronal part. Development of composites with antimicrobial properties against this bacterium is as important as obtaining efficacy against cariogenic bacteria. Originality/value: The antimicrobial effectiveness against E. faecalis of experimental composites with submicrometer-sized particles of S-P was not investigated until now.

Plasma Deposition of Biomolecules for Enhanced Biomedical Applications

2015 ◽  
Vol 1723 ◽  
Author(s):  
Liam O’Neill ◽  
Barry Twomey ◽  
Peter Dobbyn ◽  
John O’Donoghue
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Plasma Deposition ◽  
Preclinical Trial ◽  
Minimal Impact ◽  
Plasma Device ◽  
Biological Performance ◽  
The Impact

ABSTRACTBiomolecules have been traditionally immobilised onto surfaces using wet chemical techniques for various medical applications. Recent decades have seen plasma methods being used to prepare these surfaces through various forms of surface modification, but the direct exposure of biomolecules to plasma has been avoided due to fears that the molecules would be denatured by the energetic plasma species. Recent results are now demonstrating that direct plasma deposition of biomolecule coatings can be achieved. This creates the possibility to directly modify the surface of implants without any form of surface pre-treatment and this opens up the possibility to alter the healing processes. Materials such as collagen, chitosan, catalase and heparin can be effectively deposited onto surfaces with minimal impact on biological performance and without any chemical binders, linkers or impurities. The performance of these materials has been characterised using both in vitro and in vivo methodologies. In a further step, the results of a preclinical trial are presented which reveal that direct deposition of biomolecules onto open wounds can also be achieved and the impact of this on wound healing is measured in an immunocompromised animal model. A non-thermal plasma device was used to deliver collagen on to chronic wounds and the treatment was shown to promote wound closure in a rabbit wound healing model.

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