Oral Supplementation of Collagen Peptides Improves Skin Hydration by Increasing the Natural Moisturizing Factor Content in the Stratum Corneum: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

2021 ◽  
Vol 34 (3) ◽  
pp. 115-127
Author(s):  
Miho Miyanaga ◽  
Taro Uchiyama ◽  
Akira Motoyama ◽  
Nobuhiko Ochiai ◽  
Osamu Ueda ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Water Content ◽  
Transepidermal Water Loss ◽  
Skin Condition ◽  
Controlled Clinical Trial ◽  
Skin Thickness ◽  
Skin Elasticity ◽  
Oral Ingestion ◽  
Double Blind ◽  
Collagen Peptides

<b><i>Introduction:</i></b> We aimed to investigate the effect of orally ingested collagen peptides (CPs) on skin condition and elucidate their mechanism of action. <b><i>Methods:</i></b> A randomized, placebo-controlled, double-blind trial was conducted in 99 healthy Japanese women, aged 35–50 years. The subjects were randomized into 3 groups (33 subjects/group) to receive 1 or 5 g of CP or placebo once daily for 12 weeks. Skin water content, transepidermal water loss (TEWL), skin elasticity, and skin thickness were evaluated before treatment and after 4, 8, and 12 weeks of treatment. The level of natural moisturizing factor (NMF) constituents in the stratum corneum (SC) was quantified before treatment and after 12 weeks of treatment. <b><i>Results:</i></b> Oral ingestion of CP increased the water content in the SC and epidermis and decreased TEWL. Furthermore, the NMF level in the SC was increased. However, skin elasticity and skin thickness remained unchanged. <b><i>Conclusions:</i></b> The improvement in skin water content following the oral ingestion of CP can be attributed to an increase in the level of NMF in the SC. <b><i>Trial Registration:</i></b> UMIN000030375 (retrospectively registered).

scholarly journals Oral Hyaluronan Relieves Wrinkles and Improves Dry Skin: A 12-Week Double-Blinded, Placebo-Controlled Study

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2220
Author(s):  
Tzu-Fang Hsu ◽  
Zi-Rong Su ◽  
Yao-Hao Hsieh ◽  
Ming-Fu Wang ◽  
Mariko Oe ◽  
...  
Keyword(s):  
Placebo Group ◽  
Stratum Corneum ◽  
Water Content ◽  
Water Loss ◽  
Group Versus ◽  
Transepidermal Water Loss ◽  
Skin Condition ◽  
Joint Fluid ◽  
Controlled Study ◽  
Stratum Corneum Water Content

Hyaluronan (HA) is present in all connective tissues and organs, including the skin and joint fluid. However, few clinical trials have comprehensively evaluated the impacts of oral HA on skin conditions, including wrinkles and moisturization. In this study, we conducted a placebo-controlled, randomized, double-blind trial of daily HA (120 mg) intake for 12 weeks in 40 healthy Asian men and women (aged 35–64 years). Skin condition was determined by the evaluation of wrinkles, stratum corneum water content, the amount of transepidermal water loss, elasticity, and through image analysis. After 12 weeks, skin condition was significantly improved in terms of wrinkle assessment, stratum corneum water content, transepidermal water loss, and elasticity in the HA group compared to the placebo group. Regarding the percentage change from baseline, wrinkle assessment, stratum corneum water content, and skin elasticity were significantly improved in the HA group versus the placebo group after 8 and 12 weeks of ingestion. The present findings indicate that oral ingestion of HA may suppress wrinkles and improve skin condition.

Role of Centella asiatica and ceramide in skin barrier improvement: a double blind clinical trial of Indonesian batik workers

2021 ◽  
Vol 32 (4) ◽  
pp. 589-593
Author(s):  
Sylvia Anggraeni ◽  
Menul Ayu Umborowati ◽  
Damayanti Damayanti ◽  
Anang Endaryanto ◽  
Cita Rosita Sigit Prakoeswa
Keyword(s):  
Clinical Trial ◽  
Contact Dermatitis ◽  
Stratum Corneum ◽  
Skin Barrier ◽  
Centella Asiatica ◽  
Transepidermal Water Loss ◽  
Double Blind ◽  
Stratum Corneum Hydration ◽  
Double Blind Clinical Trial

Abstract Objectives Batik dyes contain irritant chemicals that increase the risk of skin barrier disruption. This study aims to determine the effect of Centella asiatica and ceramide in transepidermal water loss (TEWL), hydration of the stratum corneum and skin acidity (pH). Methods This was a double blind clinical trial of 30 Indonesian batik workers who suffered from skin dryness, but had no clinical manifestation of contact dermatitis. Subjects were given cream containing C. asiatica or ceramide that formulated and randomly labeled by manufacturer (PT Paragon Technology and Innovation). Both subjects and researchers were blinded to the type of the cream. Cream was applied to the hands and arms twice a day. Biological function of the skin (TEWL, stratum corneum hydration level, and skin acidity) was examined by Cutometer dual MP-580. Baseline was recorded in the first examination, followed by second and third examinations at two and four weeks after treatment. Results After four weeks treatment, there were significant improvement of C. asiatica application in evaluation of corneometer palmar (p=0.007; CI 95%), corneometer dorsum (p=0.001; CI 95%), and skin acidity dorsum (p=0.017; CI 95%). Ceramide application also gave significant improvement of corneometer palmar (0.038; CI 95%), skin acidity palmar (p=0.001; CI 95%), TEWL dorsum (p=0.023; CI 95%), corneometer dorsum (p=0.002; CI 95%) and skin acidity dorsum (p=0.011; CI 95%). There were no significant differences of C. asiatica effectiveness compared to ceramide in skin barrier improvement. Conclusions C. asiatica and ceramide can improve skin barrier hydration in order to prevent the risk of contact dermatitis in batik workers.

scholarly journals Moisturizing effect of a cosmetic formulation containing pequi oil (Caryocar brasiliense) from the Brazilian cerrado biome

2014 ◽  
Vol 50 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Wanessa Costa Silva Faria ◽  
Gabriel Azevedo de Brito Damasceno ◽  
Márcio Ferrari
Keyword(s):  
Stratum Corneum ◽  
Water Content ◽  
Skin Barrier ◽  
Transepidermal Water Loss ◽  
Skin Hydration ◽  
Skin Barrier Function ◽  
Brazilian Cerrado ◽  
Single Application ◽  
Caryocar Brasiliense ◽  
Stratum Corneum Water Content

Caryocar brasiliense, popularly known in Brazil as “pequi”, is a species widely distributed in the Brazilian Cerrado. The seeds are surrounded by a woody endocarp coated with a yellow fleshy mesocarp rich in oil and vitamin A, whose oil has a useful role in the treatment of skin aging and protection of human skin against UV-induced damage and skin hydration. The aim of this study was to evaluate the effect of cosmetic formulations containing pequi oil (Caryocar brasiliense) on skin hydration, after a single application. Hydration effect assessment was performed by applying the formulations under study (Control – no formulation, vehicle, and vehicle + pequi oil) onto forearm skin of 30 human volunteers. Skin capacitance and Transepidermal Water Loss (TEWL) measurements were analyzed before, and at 1, 2 and 3 hours after, a single application. Evaluation results of a single application of the vehicle containing pequi oil showed an increase in stratum corneum water content, indicating a skin moisturizing effect. Results of the evaluation of immediate effects of TEWL demonstrated that the vehicle containing pequi oil significantly increased skin moisture during the 3 h evaluation period. The formulations containing pequi oil showed clinical efficacy, increasing stratum corneum water content and enhancing skin barrier function.

scholarly journals Changes in the Diversity of Human Skin Microbiota to Cosmetic Serum Containing Prebiotics: Results from a Randomized Controlled Trial

2020 ◽  
Vol 10 (3) ◽  
pp. 91
Author(s):  
Ki-Bae Hong ◽  
Yang Hee Hong ◽  
Eun Young Jung ◽  
Kyungae Jo ◽  
Hyung Joo Suh
Keyword(s):  
Controlled Trial ◽  
Transepidermal Water Loss ◽  
Shannon Index ◽  
Water Holding Capacity ◽  
Controlled Clinical Trial ◽  
Skin Microbiota ◽  
Double Blind ◽  
Group Water ◽  
The Mean ◽  
Water Holding

Prebiotic treatment may rebalance the skin microbiota by regulating the growth of harmful and beneficial microorganisms. In this randomized, double-blind, placebo-controlled clinical trial (N = 60), we evaluated the effects of a cosmetic serum containing galacto-oligosaccharides (GOS) on the balance of the skin microbiota by measuring various skin parameters. The skin water-holding capacity between the control (ND) and experimental (NF) groups was significantly different after 8 weeks of serum treatment (p < 0.05). Similarly, changes in transepidermal water loss (TEWL) and the erythema index in the ND and NF groups were significantly different (p < 0.05). Furthermore, the wrinkle depth and Staphylococcus aureus population decreased in the NF group compared with those in the ND group (p < 0.05). The mean form factor, Shannon index, and Pediococcus population were significantly increased in the post-NF group compared with those in the post-ND group (p < 0.05). Finally, in the ND group, water-holding capacity was positively correlated with Enhydrobacter, whereas Enterobacteriaceae was negatively correlated with TEWL in the NF group. These results suggest that GOS inhibit the growth of harmful skin microbes and increase the population of beneficial microbes.

scholarly journals Cosmetic benefits of astaxanthin on humans subjects.

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Kumi Tominaga ◽  
Nobuko Hongo ◽  
Mariko Karato ◽  
Eiji Yamashita
Keyword(s):  
Moisture Content ◽  
Haematococcus Pluvialis ◽  
Basal Layer ◽  
Transepidermal Water Loss ◽  
Skin Condition ◽  
Controlled Study ◽  
Open Label ◽  
Double Blind ◽  
Oral Supplementation ◽  
Crow’S Feet

Two human clinical studies were performed. One was an open-label non-controlled study involving 30 healthy female subjects for 8 weeks. Significant improvements were observed by combining 6 mg per day oral supplementation and 2 ml (78.9 μM solution) per day topical application of astaxanthin. Astaxanthin derived from the microalgae, Haematococcus pluvialis showed improvements in skin wrinkle (crow's feet at week-8), age spot size (cheek at week-8), elasticity (crow's feet at week-8), skin texture (cheek at week-4), moisture content of corneocyte layer (cheek in 10 dry skin subjects at week-8) and corneocyte condition (cheek at week-8). It may suggest that astaxanthin derived from H. pluvialis can improve skin condition in all layers such as corneocyte layer, epidermis, basal layer and dermis by combining oral supplementation and topical treatment. Another was a randomized double-blind placebo controlled study involving 36 healthy male subjects for 6 weeks. Crow's feet wrinkle and elasticity; and transepidermal water loss (TEWL) were improved after 6 mg of astaxanthin (the same as former study) daily supplementation. Moisture content and sebum oil level at the cheek zone showed strong tendencies for improvement. These results suggest that astaxanthin derived from Haematococcus pluvialis may improve the skin condition in not only in women but also in men.

scholarly journals Influence of Oral Administration of Lactic Acid Bacteria Metabolites on Skin Barrier Function and Water Content in a Murine Model of Atopic Dermatitis

2018 ◽  
Author(s):  
Yoshihiro Tokudome
Keyword(s):  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Stratum Corneum ◽  
Water Content ◽  
Murine Model ◽  
Lipid Fraction ◽  
Transepidermal Water Loss ◽  
Water Soluble ◽  
Skin Barrier Function

The effects of orally administered lactic acid bacteria metabolites on the skin were studied using an atopic dermatitis-like murine model created by feeding mice with HR-AD. The lactic acid bacteria metabolites were obtained by inoculating 35 strains of 16 species of lactic acid bacteria into soy milk and culturing them. The atopic dermatitis-like murine model was created by feeding HR-1 mice HR-AD for 40 days. The skin condition of mice that were fed HR-AD worsened compared with normal mice, i.e., reduced water content in the stratum corneum, increased transepidermal water loss (TEWL), reduced ceramide AP content in the stratum corneum, and increased epidermis thickness. When mice that had been fed the HR-AD diet was administered a raw liquid of lactic acid bacteria metabolites orally, the measured values related to water content in the stratum corneum, TEWL, ceramide AP content in the stratum corneum, and epidermis thickness improved. To find out the active components for these effects, filtrate and residue from the raw liquid of lactic acid bacteria metabolites and lipid components extracted from the raw liquid were examined at the same time. Results showed that the water-soluble components or residue after filtration did not demonstrate effects but the raw liquid and the lipid fraction did. These findings suggest that lactic acid bacteria metabolites improve skin injury in an atopic dermatitis-like murine model.

scholarly journals Influence of Oral Administration of Lactic Acid Bacteria Metabolites on Skin Barrier Function and Water Content in a Murine Model of Atopic Dermatitis

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1858 ◽  
Author(s):  
Yoshihiro Tokudome
Keyword(s):  
Atopic Dermatitis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Stratum Corneum ◽  
Water Content ◽  
Murine Model ◽  
Lipid Fraction ◽  
Transepidermal Water Loss ◽  
Water Soluble ◽  
Skin Barrier Function

The effects of orally administered lactic acid bacteria metabolites on skin were studied using an atopic dermatitis-like murine model generated by feeding HR-AD to mice. Lactic acid bacteria metabolites were obtained by inoculating and culturing soy milk with 35 strains of 16 species of lactic acid bacteria. The atopic dermatitis-like murine model was generated by feeding HR-AD to HR-1 mice for 40 days. The skin condition of HR-AD-fed mice worsened compared with normal mice, showing reduced water content in the stratum corneum, increased transepidermal water loss (TEWL), reduced ceramide AP content in the stratum corneum, and increased epidermis thickness. When HR-AD-fed mice were orally administered a raw liquid containing lactic acid bacteria metabolites, water content in the stratum corneum, TEWL, ceramide AP content in the stratum corneum, and epidermis thickness improved. To determine the active components responsible for these effects, filtrate, residue, and lipid components extracted from the raw liquid containing lactic acid bacteria metabolites were examined. While water-soluble components and residue obtained after filtration had no effects, the lipid fraction showed similar effects to the raw liquid. These findings suggest that lactic acid bacteria metabolites improve skin injury in an atopic dermatitis-like murine model.

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