Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage

2021 ◽  
pp. 1-12
Author(s):  
Hiaki Sato ◽  
Yoshiaki Norimatsu ◽  
Satoshi Irino ◽  
Takeshi Nishikawa
Keyword(s):  
Cell Membrane ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Immunocytochemical Staining ◽  
Long Term Storage ◽  
Liquid Based Cytology ◽  
Term Storage

<b><i>Introduction/Objective:</i></b> Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. <b><i>Materials and Methods:</i></b> Sediments of cultured RAJI cells (derived from Burkitt’s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. <b><i>Results:</i></b> For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8°C) storage was used, no improvement was noted. <b><i>Conclusions:</i></b> Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted.

The Effect of Storage Temperature and Physical State on the Performance of Antisera in Radioimmunoassay after Long-Term Storage

1988 ◽  
Vol 25 (1) ◽  
pp. 89-95
Author(s):  
B A Middleton ◽  
L M Morgan ◽  
G W Aherne ◽  
V Marks
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Liquid Form ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Control Serum ◽  
Term Storage ◽  
Temperature Storage

The performance in radioimmunoassay of four antisera after storage at temperatures ranging from −40°C to room temperature, in three physical states (frozen, liquid or freeze dried) was investigated over a 3-year period. No deterioration in antiserum performance in terms of precision and accuracy of quality control serum measurement or recovery of ligand was apparent under any of the storage conditions studied. Some lowering of titre became apparent in two of the antisera over the study period. Deterioration was most marked when antiserum was stored lyophilised at room temperature. Storage of antiserum frozen confers no advantage over storage at 4°C provided precautions are taken to minimise bacterial contamination when storing antiserum in liquid form.

scholarly journals Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage

2018 ◽  
Vol 29 (1) ◽  
pp. 94-111 ◽  
Author(s):  
Tomás Barranco ◽  
Asta Tvarijonaviciute ◽  
Damián Escribano ◽  
Fernando Tecles ◽  
José J Cerón ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Room Temperature ◽  
Storage Conditions ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Long Term Storage ◽  
Reducing Ability ◽  
Trolox Equivalent ◽  
The Stability ◽  
Term Storage

Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20 ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.

scholarly journals Storage Temperature, Controlled Atmosphere, and 1-Methylcyclopropene Effects on α-Farnesene, Conjugated Trienols, and Peroxidation in Relation with Superficial Scald, Pithy Brown Core, and Fruit Quality of ‘d’Anjou’ Pears during Long-term Storage

2016 ◽  
Vol 141 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Yan Wang
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Metabolic Balance ◽  
Superficial Scald ◽  
Long Term Storage ◽  
Temperature Controlled ◽  
Term Storage

Alternatives to ethoxyquin (Etq) are needed for controlling superficial scald of ‘Anjou’ european pears (Pyrus communis) during long-term storage. The current commercial standard storage conditions [Etq + −1 °C + controlled atmosphere (CA) with 1.5 kPa O2] reduced scald occurrence compared with control fruit (−1 °C + CA) during 6–8 months storage. At 1 °C in air, 1-methylcyclopropene (1-MCP) fumigation at 0.15 µL·L−1 at harvest was more efficient on reducing scald than Etq but did not prevent scald during 6–8 months storage. The 1-MCP-treated fruit at 1 °C in air developed their ripening capacity at 20 °C following 6–8 months storage but had deceased shipping ability (softening and yellowing of fruit). Although Etq inhibition of scald was associated with the inhibition of α-farnesene oxidation to conjugated trienols (CTols); 1-MCP reduced α-farnesene synthesis and thereby the availability of substrate to oxidize to CTols. CA storage at 1.5 kPa O2 totally prevented scald and retarded the loss of shipping ability without affecting the ripening capacity of 1-MCP-treated fruit at 1 °C through further decreases in the syntheses of ethylene, α-farnesene and CTols during 6–8 months storage. In addition, 1-MCP prevented a CA-induced disorder, pithy brown core (PBC), in ‘Anjou’ pears possibly through enhancing an oxidative/reductive metabolic balance during extended storage. In conclusion, the combinations of 1 °C + 1-MCP + CA is a potential commercial alternative to Etq for scald control while allowing the 1-MCP-treated ‘Anjou’ pears to recover ripening capacity during the shelf life period after 6–8 months storage.

scholarly journals Kinetic Analysis of Stabilizing C-Phycocyanin in the Spirulina platensis Extracts from Ultrasonic Process Associated with Effects of Light and Temperature

2018 ◽  
Vol 8 (9) ◽  
pp. 1662 ◽  
Author(s):  
Woon Yong Choi ◽  
Hyeon Yong Lee
Keyword(s):  
Storage Temperature ◽  
Extraction Process ◽  
Storage Conditions ◽  
Second Order ◽  
Light Illumination ◽  
First Order ◽  
Long Term Storage ◽  
Ultrasonic Process ◽  
Term Storage

A kinetic model was developed to reflect the stability of C-Phycocyanin (C-PC) from ultrasonic extraction process under different storage conditions. The decrease of C-PC contents was most accelerated at a high temperature of 40 °C along with light illumination, resulting in ca. 60% drop of an initial concentration for two months of storage. However, ca. 93% of the initial contents remained at 4 °C without light, which would be a most favorable condition for long-term storage. It was first shown that the decrease of the residual concentrations followed second-order kinetics under light illumination. However, without light, the decrease of the C-PC contents showed first order reaction kinetics, which implies initial C-PC concentrations are important. On the contrary, initial storage temperature seemed to be more influential under light illumination. It was also first revealed that the rate of degrading the C-PC was faster with light than without light even at the same temperature, having 0.0108 (1/h) and 0.0138 (1/h) of rate constants of first order (no light) and second order kinetics (with light) at 40 °C, respectively. Moreover, the cleavage of C-PC was also found to follow the Arrhenius relationship. Therefore, this work could provide desirable storage conditions of C-PC from non-thermal ultrasonic process for long-term storage.

scholarly journals Dependence of fat acidity value on wheat grain storage conditions

2020 ◽  
Vol 17 ◽  
pp. 00107
Author(s):  
I. A. Kechkin ◽  
V. A. Ermolaev ◽  
M. V. Ivanov ◽  
A. I. Romanenko ◽  
E. A. Gurkovskaya
Keyword(s):  
Storage Temperature ◽  
Storage Period ◽  
Acid Value ◽  
Storage Conditions ◽  
Air Humidity ◽  
Wheat Grain ◽  
Storage Duration ◽  
Long Term Storage ◽  
Relative Air Humidity

The article presents the dependence of the fat acidity value (FAV) on the values of humidity and temperature, the relationship between the storage duration for wheat grain and FAV. To establish the expiration date of wheat grain during long-term storage, the author of the article considered the fat acid value (FAV) in mg of KOH. Storage temperature and relative air humidity in a desiccator affect the change (growth) of fat acidity value. The greatest changes occurred at 6th, 7th and 8th months of storage at a relative air humidity of more than 65 % and temperatures above 20 °C. At a storage temperature of 10 °C, in all cases the growth of FAV remained insignificant and was within the limits of determination accuracy. It is noted that when the relative humidity was below 60 %, while the temperature was the same as in the previous case, the FAV of wheat grain was practically unchanged through the 6-month storage period.

Effect of storage conditions on cellulose nanocrystal stability

TAPPI Journal ◽  
2014 ◽  
Vol 13 (5) ◽  
pp. 53-61 ◽  
Author(s):  
STEPHANIE BECK ◽  
JEAN BOUCHARD
Keyword(s):  
Physical Stability ◽  
Storage Period ◽  
Storage Conditions ◽  
Neutral Salt ◽  
Long Term Storage ◽  
Industrial Material ◽  
Freeze Dried ◽  
Kinetics Of ◽  
Term Storage

Cellulose nanocrystals (CNCs) have evolved from a laboratory curiosity to an industrial material manufactured at a scale of up to 1 ton/day. Such large quantities of CNCs will inevitably be stored for different lengths of time before shipping and use. The chemical and physical stability of CNCs during long-term storage under various conditions was monitored. As-produced acidic H-CNCs and neutral salt form Na-CNCs were stored at ambient temperature and at 4°C as never-dried suspensions, and as a freeze-dried solid in the case of Na-CNCs. A variety of parameters were measured at intervals during the storage period. The CNC sulfate half-ester content, the cellulose chain length, and the unique optical properties of CNC films were of particular interest. Changes in these parameters were analyzed to determine the kinetics of long-term CNC degradation and establish the shelf-life of CNCs under different storage conditions.

The effect of storage temperature and duration on northern red oak acorn viability and vigour

2013 ◽  
Vol 89 (06) ◽  
pp. 769-776 ◽  
Author(s):  
Thomas L. Noland ◽  
Andrée E. Morneault ◽  
Daniel C. Dey ◽  
Dave Deugo
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Red Oak ◽  
Northern Red Oak ◽  
Root Regrowth ◽  
Long Term Storage ◽  
High Germination ◽  
Percent Germination ◽  
Term Storage

Three separate collections of Ontario sources of northern red oak (Quercus rubra L.) acorns were made to determine the effects of long-term cold storage at +2°C, −1°C, and −2°C on their viability and vigour. We measured acorn moisture content, percent germination during storage, speed of germination and total germination values, root regrowth of seeds that germinated in storage, incidence of fungal contamination, and condition of ungerminated acorns. Viability and vigour peaked six to 12 months after acorns were placed in storage, but decreased with continued storage. After 18 months in storage, ≥60% of the acorns germinated in four of the five seedlots tested and, after 30 months in storage, ≥53% of the acorns germinated in three of the five seedlots tested. Acorn viability was only minimally affected by storage temperature; however, since temperatures above −2°C allowed acorns to germinate during storage, the preferred long-term storage temperature was −2°C. Our results suggest that, assuming proper storage conditions, most red oak seedlots will maintain relatively high germination levels when stored at −2°C for 18 months. Seedlots with particularly high initial germination and vigour may be successfully stored for up to 30 months.

Survival of mycorrhizal fungal isolates stored in sterile water at two temperatures and retrieved on solid and liquid nutrient media

1994 ◽  
Vol 40 (9) ◽  
pp. 736-742 ◽  
Author(s):  
Jane E. Smith ◽  
Donaraye McKay ◽  
Randy Molina
Keyword(s):  
Storage Temperature ◽  
Storage Period ◽  
Nutrient Media ◽  
Long Term Storage ◽  
Fungal Isolates ◽  
Solid Media ◽  
Two Temperatures ◽  
Culture Maintenance ◽  
Term Storage

Vegetatively colonized agar cores of 169 reputed mycorrhizal fungal isolates, representing 18 Ascomycotina, 141 Basidiomycotina, and 10 Deuteromycotina fungi, were stored at 4 and 18 °C in tubes of sterile deionized water. Viability, based on growth on solid and liquid nutrient media at 18 °C, was determined after a 20-month storage period. Ninety-five percent of the isolates were retrieved in at least one of three treatments: 89% in liquid nutrient media and 84% on solid media after storage at 18 °C, and 78% on solid media after storage at 4 °C. Nearly all Ascomycotina and Deuteromycotina isolates were retrieved regardless of the storage temperature or form of retrieval medium. Basidiomycotina isolates differed more in survival and percentage of viability than Ascomycotina or Deuteromycotina. For example, storage temperature influenced the viability of Laccaria isolates and the form of retrieval medium influenced the viability of Rhizopogon isolates.Key words: culture maintenance, fungal storage, long-term storage, mycorrhizal fungal isolates, water storage.

Human plasma stability during handling and storage: impact on NMR metabolomics

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1168-1177 ◽  
Author(s):  
Joana Pinto ◽  
M. Rosário M. Domingues ◽  
Eulália Galhano ◽  
Cristina Pita ◽  
Maria do Céu Almeida ◽  
...  
Keyword(s):  
Human Plasma ◽  
Room Temperature ◽  
Storage Conditions ◽  
Plasma Composition ◽  
Long Term Storage ◽  
Short And Long Term ◽  
The Stability ◽  
And Storage ◽  
Term Storage

The stability of human plasma composition was investigated by NMR, considering different collection tubes, time at room temperature (RT), short- and long-term storage conditions and up to 5 consecutive freeze–thaw cycles.

scholarly journals Investigation of stability of intrauterine aerosol preparation “Yodozol”

2018 ◽  
Vol 20 (92) ◽  
pp. 29-33
Author(s):  
R. M. Sachuk ◽  
O. A. Katsaraba
Keyword(s):  
Shelf Life ◽  
Medicinal Product ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Analgesic Effects ◽  
Aerosol Preparation ◽  
Long Term Storage ◽  
The Stability ◽  
Term Storage

For standardization, quality control, study of stability and establishment of storage conditions and terms of use, complex preclinical trials of the new development of PE “Biopharm” and the Experimental Station of Epizootology IVM NAAN – aerosol preparation “Yodozol” have been carried out. The methods of evaluation of a medicinal product applied to aerosols are used, which include: determination of changes in appearance, inspection of packaging for leakproofness, measurement of the percentage of contents of the package, the establishment of qualitative and quantitative indicators of active substances, and also the study of microbiological purity of the product. “Yodozol” is a light yellow liquid, 1 ml of which contains 5 mg of iodine and 10 mg of potassium iodide. The drug is used for the prevention and treatment of postnatal intrauterine infections in cows, pigs, sheep and goats (endometritis, pyrometers, cervicitis, vaginitis, delayed digestion caused by microorganisms sensitive to iodine), after obstetrics aid, cesarean section and postpartum sanitation of the uterus. The drug has antimicrobial, anti-inflammatory and analgesic effects, improves the proliferative processes of the genital organs, reduces the time for recovery of animals. The drug is used according to the guidelines, after its production livestock is used without restrictions. The shelf-life, which is the result of the test of the dasg according to the «stability» indicator, has been determined, which was performed under long-term storage in a place protected from light at a temperature range from + 5 ± 2 °С to + 25 ± 2 °С. The studies conducted after 6, 12, 24 and 30 months showed complete compliance of the quality indices with the declared standards when stored for 24 months in the temperature corridor from + 5 °C to + 20 °C. With an increase in storage temperature to + 25 °C or more, a slight quantitative decrease in the concentration of antimicrobial components occurred. In addition, with long-term storage of drugs, release of the contents from the cylinder became uneven and foam acquired a shade less than the saturation rate, increased microbiological contamination. Thus, according to the results of the study, the established shelf life of the preparation is 2 years at the recommended storage temperature from +5 to           +20 °С. All studies conducted on the stability of the aerosol intrauterine drug “Yodozol” were included in the registration materials of the medicinal product.

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