scholarly journals СПЕКТРОФОТОМЕТРИЧНЕ ВИЗНАЧЕННЯ КАРВЕДІЛОЛУ В ТАБЛЕТКАХ

2015 ◽  
Author(s):  
Ю. М. Жук ◽  
С. О. Васюк ◽  
І. С. Верьовкіна
Keyword(s):  
Spectrophotometric Determination ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Spectrophotometric Analysis ◽  
Pharmaceutical Drugs ◽  
Optimal Conditions ◽  
Colored Product ◽  
Medical University ◽  
Adrenergic Blocking

<p><strong>SPECTROPHOTOMETRIC DETERMINATION OF CARVEDILOL IN TABLETS</strong></p><p><strong>Y. Zhuk, S. Vasjuk, I. Ver</strong><strong>оvkina</strong></p><p>Zaporozhye State Medical University, Zaporozhye</p><p><strong>Summary:</strong> in this investigation a visible spectrophotometric method for the determination of carvedilol based on the absorbance of colored product of the reaction between carvedilol and bromcresol purple in acetone medium at 399 nm measurement was developed. The optimal conditions for the quantitative determination of carvedilol in the content of pharmaceutical drugs were established. The validation of the worked out procedure on such validated characteristics as linearity, precision, accuracy and robustness was carried out.</p><p><strong>Keywords:</strong> spectrophotometric analysis, carvedilol, bromcresol purple.</p><p><strong>Introduction. </strong>Carvedilol (1-(9H-carbazol-4-yloxy)-3-[2-(2-methoxyphenoxy)ethylamino]propan-2-2) belongs to a group of medicines called beta-adrenergic blocking agents, that are indicated for the treatment of hypertension angina pectoris and heart failure.</p><p>Our aim is to develop a simple, accurate and valid method for the spectrophotometric determination of carvedilol.</p><p>Purposes:</p><ul><li>establish optimal conditions of the photometric reaction between carvedilol and bromcresol purple;</li><li>develop the method for the determination of carvedilol in pharmaceutical formulations;</li><li>carry out validation of the worked out procedure.</li></ul><p><strong>Methods of analysis.</strong></p><p>Object of research: tablets «Corvazan» 25 mg (Arterium), tablets «Coriol» 12,5 mg (KRKA), tablets «Coriol» 25 mg (KRKA).</p><p>Reagents and Chemicals: standard solution of carvedilol, bromcresol purple, acetone. All reagents and chemicals used were of analytical grade.</p><p>Instrument: spectrophotometer Specord 200, electronic balance ABT-120-5PM, ultrasonic bath ELMASONIC 60Н.</p><p><strong>Results and discussion</strong>.</p><p>Linearity. The analytical parameters such as molar absorptivity, Beer’s law limits and Sandell’s sensitivity values were calculated.</p><p>Precision. The developed methods give the result with repeatability sufficient for dependable determination the investigated substance in pharmaceutical formulations.</p><p>Accuracy. Accuracy established by analyte addition technique.</p><p>Robustness. Determined factors that influence on the absorbance value: reagent quantity and timing stability.</p><p>Sample solutions stable during 30 min.</p><p>Addition to sample solution ±10 % bromcresol purple solution is not change the absorbance value.</p><p><strong>Conclusions. </strong>Established that reaction between carvedilol and bromcresol purple proceeds in acetone medium at room temperature.</p><p>Molar absorption coefficient is 2,13·10<sup>4</sup>.<strong></strong></p><p>Developed the method of spectrophotometric determination of carvedilol in pharmaceutical formulations.</p><p>Proved that the method is valid on such validated characteristics as linearity, precision, accuracy and robustness. </p>

scholarly journals Spectrophotometric determination of sotalol in tablets

2018 ◽  
pp. 49-55
Author(s):  
Y. M. Zhuk ◽  
S. O. Vasyuk
Keyword(s):  
Quantitative Determination ◽  
Room Temperature ◽  
Cost Effective ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pharmaceutical Drugs ◽  
Analysis Method ◽  
Highly Sensitive ◽  
Colored Product

In this investigation a visible spectrophotometric method for the determination of sotalol based on the absorbance of colored product of the reaction between sotalol hydrochloride and bromcresol purple in acetone medium at 399 nm measurement was developed. The optimal conditions for the quantitative determination of sotalol hydrochloride in the content of pharmaceutical drugs were established. The stoichiometric relationship coefficients between sotalol hydrochloride and bromcresol purple were determined. The validation of the worked out procedure on such validated characteristics as linearity, precision, accuracy and robustness was carried out. The aim. To develop a highly sensitive, easy to use, cost-effective and valid method for quantitative determination of sotalol hydrochloride in dosage forms. The analysis method. Visible spectrophotometry. The analytical parameters such as molar absorptivity, Beer’s law limits and Sandell’s sensitivity values were calculated. The developed methods give the result with repeatability sufficient for dependable determination the investigated substance in pharmaceutical formulations. Accuracy established by analyte addition technique. Determined factors that influence on the absorbance value: reagent quantity and timing stability. Sample solutions stable during 30 min. Addition to sample solution ± 10% bromcresol purple solution is not change the absorbance value. Established that reaction between sotalol hydrochloride and bromcresol purple proceeds in acetone medium at room temperature. Molar absorption coefficient is 2,62∙103.

scholarly journals Spectrophotometric method for the determination of Captopril in pharmaceutical formulations

2013 ◽  
Vol 10 (3) ◽  
pp. 965-970
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aqueous Solution ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Maximum Absorption ◽  
Neutral Medium ◽  
Pharmaceutical Tablets ◽  
Colored Product ◽  
Sensitive Spectrophotometric Method

A simple, rapid and sensitive spectrophotometric method has been developed for the determination of captopril in aqueous solution. The method is based on reaction of captopril with 2,3-dichloro 1,4- naphthoquinon(Dichlone) in neutral medium to form a stable yellow colored product which shows maximum absorption at 347 nm with molar absorptivity of 5.6 ×103 L.mole-1. cm-1. The proposed method is applied successfully for determination of captopril in commercial pharmaceutical tablets.

Liquid–liquid extraction and cloud point extraction for spectrophotometric determination of vanadium using 4-(2-pyridylazo)resorcinol

2015 ◽  
Vol 69 (4) ◽  
Author(s):  
Teodora S. Stefanova ◽  
Kiril K. Simitchiev ◽  
Kiril B. Gavazov
Keyword(s):  
Cloud Point ◽  
Spectrophotometric Determination ◽  
Cloud Point Extraction ◽  
Limit Of Detection ◽  
Molar Absorptivity ◽  
Liquid Extraction ◽  
Optimal Conditions ◽  
Liquid Liquid Extraction ◽  
Relative Standard

AbstractLiquid-liquid extraction (LLE) and cloud point extraction (CPE) of vanadium(V) ternary complexes with 4-(2-pyridylazo)resorcinol (PAR) and 2,3,5-triphenyl-2H-tetrazolum chloride (TTC) were investigated. The optimal conditions for vanadium extraction and spectrophotometric determination were identified. The composition (V : PAR : TTC) of the extracted species was 1 : 2 : 3 (optimal conditions; LLE), 2 : 2 : 2 (low reagents concentrations; LLE), 1 : 1 : 1 (short heating time; CPE), and 1 : 1 : 1 + 1 : 1 : 0 (optimal extraction conditions; CPE). LLE, performed in the presence of 1,2-diaminocyclohexane-N,N,N’,N’-tetraacetic acid and NH4F as masking agents, afforded the sensitive, selective, precise, and inexpensive spectrophotometric determination of vanadium. The absorption maximum, molar absorptivity, limit of detection, and linear working range were 559 nm, 1.95 × 105 dm3 mol−1 cm−1, 0.7 ng cm−3, and 2.2-510 ng cm−3, respectively. The procedure thus developed was applied to the analysis of drinking waters and steels. The relative standard deviations for V(V) determination were below 9.4 % (4-6 × 10−7 mass %; water samples) and 2.12 % (1-3 mass %; steel samples).

scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DPP-4 INHIBITOR, SITAGLIPTIN, IN ITS PHARMACEUTICAL PREPARATIONS

2018 ◽  
Vol 35 (3) ◽  
pp. 45
Author(s):  
C. Bala Sekaran ◽  
A. Prameela Rani
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Amino Group ◽  
Reaction Conditions ◽  
Colored Product ◽  
Primary Amino ◽  
Development And Validation

A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer’s law is obeyed over a concentration range of 5-25 μg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 104 Lmol-1cm-1 and 0.0471 μgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.

scholarly journals Novel Spectrophotometric Methods for the Determination of Selegiline Hydrochloride in Bulk and Its Pharmaceutical Preparation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kumble Divya ◽  
Badiadka Narayana
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Spectrophotometric Methods ◽  
Colored Product ◽  
Optimum Reaction ◽  
Optimum Reaction Condition

A simple and highly selective spectrophotometric method has been developed for the determination of selegiline hydrochloride in bulk and formulations. Method A is based on the oxidation of 3-methyl-2-benzothiazolinone hydrazone in the presence of ceric ammonium sulphate, followed by its coupling reaction with drug to form a colored product having λmax of 629 nm. Method B is based on the coupling reaction of drug with 4-aminoantipyrine to give a new ligand that reacts with copper(II) to give intense bluish red colored chelate which is measured at 539 nm. Beer’s law is obeyed in the range of 10.00–85.00 μg mL−1 with molar absorptivity of 0.98×104 for method A and 20.00–120.00 μg mL−1 with molar absorptivity of 0.94×104 for method B. The optimum reaction condition and the analytical parameters are evaluated. The results obtained indicate that the methods are free from interference of the ingredients; thus they are successfully applied to pharmaceutical formulations.

scholarly journals Simple UV Spectrophotometric Determination of Rosuvastatin Calcium in Pure Form and in Pharmaceutical Formulations

2009 ◽  
Vol 6 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Alka Gupta ◽  
P. Mishra ◽  
K. Shah
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Ultraviolet Region ◽  
Rosuvastatin Calcium ◽  
Sensitive Spectrophotometric Method

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of rosuvastatin calcium in bulk and in pharmaceutical formulations. Rosuvastatin exhibits absorption maxima at 244 nm with apparent molar absorptivity of 7.2345 ×104L/mol.cm in methanol. Beer’s law was found to be obeyed in the concentration range of 2-18 µg/mL. The method is accurate, precise and economical. This method is extended to pharmaceutical preparations. In this method, there is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated statistically and by recovery studies

scholarly journals Spectrophotometric determination of Valsartan in pure form and in its pharmaceutical preparations

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Qabas Rashid ◽  
Ruwaida Farman Salih
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Chromogenic Reagent

An easy, rapid and economical spectrophotometric method for  determination of  Valsartan (Val), by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) as reagent in an alkaline interemediate. This method is based on the forming of product between (Val) and the chromogenic reagent (NBD-Cl), to produce a brown color at (pH 11.9) and λmax. 470 nm.  Beer’s Law is obeyed at the concentrations range of (0.4-14.8 µg/ml), with molar absorptivity of (1.05×104 L/mol.cm) and correlation coefficient 0.9827, The limit of detection was 0.557 µg/ml. The suggested method was prosperity implement to the determination of (Val) in  pure form and in its pharmaceutical formulations (tablets).

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF NEVIRAPINE USING TETRATHIOCYANATOCOBALT(II) ION AS A REAGENT

2018 ◽  
Vol 35 (3) ◽  
pp. 93
Author(s):  
T. V. Badiadka ◽  
Badiadka Narayana
Keyword(s):  
Detection Limit ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Statistical Evaluation ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Optical Parameters ◽  
Quantitation Limit ◽  
Optimum Reaction

A simple and rapid spectrophotometric method for the determination of nevirapine is described. The method is based on the reaction of nevirapine with tetrathiocyanatocobalt(II) ion in buffer of pH 4 to form the corresponding complex. Beer’s law is obeyed in the range of 0.2 – 2.0 μg mL-1 for nevirapine. The optical parameters such as molar absorptivity, Sandell’s sensitivity, detection limit and quantitation limit were found to be 1.16× 104 Lmol-1cm-1, 2.09 X 10-3 μg cm-2, 0.073 μg mL-1 and 0.222 μg mL-1 respectively. The optimum reaction conditionsand other analytical parameters were evaluated. The statistical evaluation of the method was examined by determining intra-day and inter-day precision. The proposed method has been successfully applied for the determination of nevirapine in pharmaceutical formulations.

scholarly journals Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent

2003 ◽  
Vol 68 (8-9) ◽  
pp. 685-690 ◽  
Author(s):  
Dhruv Singh ◽  
Bhavana Srivastava ◽  
Archana Sahu
Keyword(s):  
Alkaline Medium ◽  
Spectrophotometric Determination ◽  
Molar Absorptivity ◽  
Spectrophotometric Procedure ◽  
Chromogenic Reaction ◽  
Colored Product

Arapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue colored product due to reduction of tungstate and/or molybdate in Folin Ciocalteu?s reagent (FCR) by ajmaline and brucine in alkaline medium. The color is stable for more than 48 h. The chromogenic reaction has ?max at 540 nm with molar absorptivity 1.64 x 104 and 2.37 x 103 l mol-1 cm-1 in the Beer?s law range 1?8 ?g ml-1 and 10?100 ?g ml-1 for ajmaline and brucine, respectively.

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