scholarly journals DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DPP-4 INHIBITOR, SITAGLIPTIN, IN ITS PHARMACEUTICAL PREPARATIONS

2018 ◽  
Vol 35 (3) ◽  
pp. 45
Author(s):  
C. Bala Sekaran ◽  
A. Prameela Rani
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Amino Group ◽  
Reaction Conditions ◽  
Colored Product ◽  
Primary Amino ◽  
Development And Validation

A simple, sensitive and reproducible spectrophotometric method was developed for the determination of sitagliptin phosphate in bulk and in pharmaceutical formulations. The proposed method is based on condensation of the primary amino group of sitagliptin phosphate with acetyl acetone and formaldehyde producing a yellow colored product, which is measured spectrophotometrically at 430nm. The color was stable for about 1 hour. Beer’s law is obeyed over a concentration range of 5-25 μg/ml. The apparent molar absorptivity and Sandell sensitivity values are 1.067 x 104 Lmol-1cm-1 and 0.0471 μgcm-2 respectively. All the variables were studied to optimize the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of the method was tested by analyzing sitagliptin phosphate in its pharmaceutical preparations. Good recoveries were obtained. The developed method was successfully employed for the determination of sitagliptin phosphate in various pharmaceutical preparations.

scholarly journals Spectrophotometric method for the determination of Captopril in pharmaceutical formulations

2013 ◽  
Vol 10 (3) ◽  
pp. 965-970
Author(s):  
Baghdad Science Journal
Keyword(s):  
Aqueous Solution ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Maximum Absorption ◽  
Neutral Medium ◽  
Pharmaceutical Tablets ◽  
Colored Product ◽  
Sensitive Spectrophotometric Method

A simple, rapid and sensitive spectrophotometric method has been developed for the determination of captopril in aqueous solution. The method is based on reaction of captopril with 2,3-dichloro 1,4- naphthoquinon(Dichlone) in neutral medium to form a stable yellow colored product which shows maximum absorption at 347 nm with molar absorptivity of 5.6 ×103 L.mole-1. cm-1. The proposed method is applied successfully for determination of captopril in commercial pharmaceutical tablets.

scholarly journals Development and validation of a simple spectrophotometric method for the determination of methyldopa in both bulk and marketed dosage formulations

2014 ◽  
Vol 50 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Paulo Roberto da Silva Ribeiro ◽  
Reginária Morais Duarte
Keyword(s):  
Sodium Nitrite ◽  
Spectrophotometric Method ◽  
Confidence Level ◽  
Absorption Maximum ◽  
Pharmaceutical Preparations ◽  
Coefficient Of Determination ◽  
Colored Product ◽  
Development And Validation ◽  
Nitrosation Reaction

A simple, precise, sensitive, rapid, specific and economical spectrophotometric method was developed to determine methyldopa (MTD) content in bulk and pharmaceutical dosage formulations. The proposed method was based on the formation of a colored product from the nitrosation reaction of MTD with sodium nitrite in an acid medium. The resultant nitroso derivative species reacts further with sodium hydroxide and is converted it into a more stable compound. This yellow nitrosation product exhibited an absorption maximum at 430 nm. Beer's Law was obeyed in a concentration range of 6.37 to 82.81 μg mL-1 MTD with an excellent coefficient of determination (R2 = 0.9998). No interference was observed from common excipients in formulations. The results showed the method to be simple, accurate and readily applied for the determination of MTD in pure form and in pharmaceutical preparations. The analytical results obtained for these products using the proposed method are in agreement with those of the Brazilian Pharmacopoeia procedure at a 95% confidence level.

scholarly journals Leishman’s Dye as a Novel Reagent in Spectrophotometric Determination of Chloramphenicol

2020 ◽  
Vol 10 (02) ◽  
pp. 250-254
Author(s):  
Jamal Sudad Raeek Othman Nabeel Sabeeh
Keyword(s):  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Pharmaceutical Preparations ◽  
High Sensitivity ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Sensitivity Index ◽  
Oxidizing Agent ◽  
Drug Compounds

By reviewing the literature, there is no indication concerning the use of Leishman’s dye in evaluating drug compounds by dye-color bleaching; hence, it is the first attempt to use Leishman’s dye as a novel reagent in the estimation of chloramphenicol (CAP) by an indirect spectrophotometric method in bulk and in its pharmaceutical preparations. The method includes the use of a great amount of N-bromosuccinamide (NBS) in the acidic medium as an oxidizing agent of the drug under investigation (CAP), and then using the residual of NBS for Leishman’s dye color bleaching. The absorbance has been measured at 622 nm (the maximum absorption of Leishman’s dye). A linear relationship was obtained for the Beer’s law with the concentration ranges from 10 to 250 μg/10 mL with acceptable values of molar absorptivity 0.58 × 104 L.mol-1.cm-1 and 0.055 μg.cm-2 of Sandell’s sensitivity index, which mean a high sensitivity. An approved estimation of CAP in its various pharmaceutical formulations was found.

scholarly journals Novel Spectrophotometric Methods for the Determination of Selegiline Hydrochloride in Bulk and Its Pharmaceutical Preparation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Kumble Divya ◽  
Badiadka Narayana
Keyword(s):  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Spectrophotometric Methods ◽  
Colored Product ◽  
Optimum Reaction ◽  
Optimum Reaction Condition

A simple and highly selective spectrophotometric method has been developed for the determination of selegiline hydrochloride in bulk and formulations. Method A is based on the oxidation of 3-methyl-2-benzothiazolinone hydrazone in the presence of ceric ammonium sulphate, followed by its coupling reaction with drug to form a colored product having λmax of 629 nm. Method B is based on the coupling reaction of drug with 4-aminoantipyrine to give a new ligand that reacts with copper(II) to give intense bluish red colored chelate which is measured at 539 nm. Beer’s law is obeyed in the range of 10.00–85.00 μg mL−1 with molar absorptivity of 0.98×104 for method A and 20.00–120.00 μg mL−1 with molar absorptivity of 0.94×104 for method B. The optimum reaction condition and the analytical parameters are evaluated. The results obtained indicate that the methods are free from interference of the ingredients; thus they are successfully applied to pharmaceutical formulations.

scholarly journals Simple UV Spectrophotometric Determination of Rosuvastatin Calcium in Pure Form and in Pharmaceutical Formulations

2009 ◽  
Vol 6 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Alka Gupta ◽  
P. Mishra ◽  
K. Shah
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Ultraviolet Region ◽  
Rosuvastatin Calcium ◽  
Sensitive Spectrophotometric Method

A new, simple and sensitive spectrophotometric method in ultraviolet region has been developed for the determination of rosuvastatin calcium in bulk and in pharmaceutical formulations. Rosuvastatin exhibits absorption maxima at 244 nm with apparent molar absorptivity of 7.2345 ×104L/mol.cm in methanol. Beer’s law was found to be obeyed in the concentration range of 2-18 µg/mL. The method is accurate, precise and economical. This method is extended to pharmaceutical preparations. In this method, there is no interference from any common pharmaceutical additives and diluents. Results of the analysis were validated statistically and by recovery studies

scholarly journals Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage forms

2008 ◽  
Vol 58 (3) ◽  
pp. 275-285 ◽  
Author(s):  
Basavaraj Hiremath ◽  
Bennikallu Mruthyunjayaswamy
Keyword(s):  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Color Intensity ◽  
Reaction Conditions ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods ◽  
Colored Product ◽  
Ethylenediamine Dihydrochloride ◽  
Development And Validation

Development and validation of spectrophotometric methods for determination of ceftazidime in pharmaceutical dosage formsTwo spectrophotometric methods for the determination of ceftazidime (CFZM) in either pure form or in its pharmaceutical formulations are described. The first method is based on the reaction of 3-methylbenzothiazolin-2-one hydrazone (MBTH) with ceftazidime in the presence of ferric chloride in acidic medium. The resulting blue complex absorbs at λmax628 nm. The second method describes the reaction between the diazotized drug andN-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to yield a purple colored product with λmaxat 567 nm. The reaction conditions were optimized to obtain maximum color intensity. The absorbance was found to increase linearly with increasing the concentration of CFZM; the systems obeyed the Beer's law in the range 2-10 and 10-50 μg mL-1for MBTH and NEDA methods, resp.LOD, LOQand correlation coefficient values were 0.15, 0.79 and 0.50, 2.61. No interference was observed from common excipients present in pharmaceutical formulations. The proposed methods are simple, sensitive, accurate and suitable for quality control applications.

scholarly journals Determination of Metochloropramide Hydrochloride by Spectrophotometric Method by using Diazotized p-nitro aniline reagent

2020 ◽  
Vol 10 (01) ◽  
pp. 68-73
Author(s):  
Intidhar D. Sulaiman ◽  
Walaa A. Abd Alrada
Keyword(s):  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Azo Dye ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Molar Absorptivity ◽  
Water Soluble ◽  
Amino Group ◽  
Primary Amino

New simple, sensitive, accurate, and inexpensive spectrophotometric technique has been developed for the estimation of Metochloropramide hydrochloride [MCP-HCl] in pure and pharmaceutical preparation. This technique is based on the diazotization of a primary amino group of Metochloropramide hydrochloride [MCP-HCl] with NaNo2 and HCl followed by coupling with p-nitro aniline in alkaline medium to obtain a stable red-colored water-soluble azo-dye, show a maximum absorption (λmax) at 513. 50nm. Bear,s low is obeyed in the concentration range of (0.2–25) μg.ml-1 with molar absorptivity of 2.313×103L.mol-1.cm-1and sandall’s sensitivity 0.0145 μg.mL-2. The limit of detection (LOD) and limit of quantitative were 0.182 μg.mL-1and 0.553 μg.mL-1, respectively. The proposed technique successfully applied to (tablets, syrup, and injection).

scholarly journals Spectrophotometric determination of Valsartan in pure form and in its pharmaceutical preparations

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Qabas Rashid ◽  
Ruwaida Farman Salih
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Chromogenic Reagent

An easy, rapid and economical spectrophotometric method for  determination of  Valsartan (Val), by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) as reagent in an alkaline interemediate. This method is based on the forming of product between (Val) and the chromogenic reagent (NBD-Cl), to produce a brown color at (pH 11.9) and λmax. 470 nm.  Beer’s Law is obeyed at the concentrations range of (0.4-14.8 µg/ml), with molar absorptivity of (1.05×104 L/mol.cm) and correlation coefficient 0.9827, The limit of detection was 0.557 µg/ml. The suggested method was prosperity implement to the determination of (Val) in  pure form and in its pharmaceutical formulations (tablets).

scholarly journals New Kinetic Spectrophotometric Method for Determination of Fexofenadine Hydrochloride in Pharmaceutical Formulations

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Safwan Ashour ◽  
Mouhammed Khateeb
Keyword(s):  
Spectrophotometric Method ◽  
Initial Rate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Fixed Time ◽  
Official Method ◽  
Reaction Conditions ◽  
Accuracy And Precision ◽  
Kinetic Spectrophotometric

A simple and sensitive kinetic spectrophotometric method was developed for the determination of fexofenadine hydrochloride in bulk and pharmaceutical preparations. The method is based on a kinetic investigation of the oxidation reaction of fexofenadine using alkaline potassium permanganate as an oxidizing agent at room temperature. The reaction is followed spectrophotometrically by measuring the increase of absorbance owing to the formation of manganate ion at 610 nm. The initial rate and fixed time (at 15 min) methods are utilized for construction of calibration graphs. All the reaction conditions for the proposed method have been studied. The linearity range was found to be 2.5–50.0 μg mL−1 with detection limit of 0.055 μg mL−1 for both initial rate and fixed time methods. The proposed method was applied successfully for the determination of fexofenadine in pharmaceutical formulations; the percentage recoveries were 99.98–101.96%. The results obtained were compared statistically with those obtained by the official method and showed no significant differences regarding accuracy and precision.

scholarly journals Estimation of Furosemide in Pharmaceutical Preparation Samples

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Oral Solution ◽  
Average Recovery ◽  
Relative Standard

A simple, accurate, precise, rapid, economical and sensitive ultraviolet spectrophotometric method has been developed for the determination of Furosemide in pharmaceutical preparations, which shows maximum absorbance at 228 nm in. Beer’s law was obeyed in the range of 1 -10 μg/ ml, with molar absorptivity of 3.5×104 L.mol-1.cm-1, relative standard deviation of the method was less than 1.4%, and accuracy (average recovery %) was 100 ± 1.0. The method was successfully applied to the determination of Furosemide in some pharmaceutical formulations (tablets, Oral solution and injection) samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Furosemide in true samples.

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