Effect of Graded Hypoxia on the Induction and Function of Inducible Nitric Oxide Synthase in Rat Mesangial Cells

1995 ◽  
Vol 77 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Stephen L. Archer ◽  
Kimberly A. Freude ◽  
Pamela J. Shultz
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Mesangial Cells ◽  
And Function ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Effects of p38MAPK isoforms on renal mesangial cell inducible nitric oxide synthase expression

2004 ◽  
Vol 286 (1) ◽  
pp. C145-C152 ◽  
Author(s):  
Paul Lui ◽  
Chenbo Zeng ◽  
Stephen Acton ◽  
Steven Cok ◽  
Alison Sexton ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Inos Expression ◽  
Chimeric Proteins ◽  
Wild Type ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Several related isoforms of p38MAPK have been identified and cloned in many species. Although they all contain the dual phosphorylation motif TGY, the expression of these isoforms is not ubiquitous. p38α and -β2 are ubiquitously expressed, whereas p38γ and -δ appear to have more restricted expression. Because there is evidence for selective activation by upstream kinases and selective preference for downstream substrates, the functions of these conserved proteins is still incompletely understood. We have demonstrated that the renal mesangial cell expresses the mRNA for all the isoforms of p38MAPK, with p38α mRNA expressed at the highest level, followed by p38γ and the lowest levels of expression by p38β2 and -δ. To determine the functional effects of these proteins on interleukin (IL)-1β-induced inducible nitric oxide synthase (iNOS) expression, we transduced TAT-p38 chimeric proteins into renal mesangial cells and assessed the effects of wild-type and mutant p38 isoforms on ligand induced iNOS expression. We show that whereas p38γ and -δ had minimal effects on iNOS expression, p38α and -β2 significantly altered its expression. p38α mutant and p38β2 wild-type dose dependently inhibited IL-1β-induced iNOS expression. These data suggest that p38α and β2 have reciprocal effects on iNOS expression in the mesangial cell, and these observations may have important consequences for the development of selective inhibitors targeting the p38MAPK family of proteins.

scholarly journals Endothelin-1 inhibits cytokine-stimulated transcription of inducible nitric oxide synthase in glomerular mesangial cells

1995 ◽  
Vol 48 (6) ◽  
pp. 1893-1899 ◽  
Author(s):  
Karl-Friedrich Beck ◽  
Markus G. Mohaupt ◽  
R. Bernd Sterzel ◽  
Stephanie with technical assistance from Peters ◽  
Hans Fees
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Mesangial Cells ◽  
Glomerular Mesangial Cells ◽  
Endothelin 1 ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
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