Abstract 544: Diabetes Mellitus Impacts on Vascular Calcification in Peripheral Arterial Disease Patients but Does Not Further Activates Osteogenic Markers in Beta-Glycerophosphate Stimulated Vascular Smooth Muscle Cells

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Elisangela Farias-Silva ◽  
Cynthia A Mendes ◽  
Luciana A Pescatore ◽  
Maria Claudina C Andrade ◽  
Luciana S Carmo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Smooth Muscle ◽  
Peripheral Arterial Disease ◽  
Protein Expression ◽  
Smooth Muscle Cells ◽  
Mrna Expression ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Arterial Disease ◽  
Peripheral Arterial

Introduction: Diabetes mellitus (DM) accelerates vascular calcification (VC) in peripheral arterial disease (PAD), increasing limb ischemia and amputation risk. Although DM and VC implicates in PAD, the mechanisms underlying vascular smooth muscle cells (SMC) osteochondrogenic differentiation in this setting are scarce. Objectives: To assess VC and osteogenic mRNA/protein expression (Msx2, Runx2 and ALPL) in arteries and in primary SMC isolated from PAD patients with DM (n=3), without DM (n=2) and individuals without PAD (control [CTL], n=2) that underwent amputation. Methods: Ethical committee approved study and patients signed informed consent. 5-7 th passages SMC were incubated without (untreated) or with β-glycerophosphate 10mM (β-GP) for 48h (mRNA expression), 72h (protein expression) and 14 days (calcification). In addition, we performed tissue immunofluorescence and Alizarin Red S. Results: Untreated PAD+DM SMC increased calcification (4.6±1.2) in comparison to PAD without DM patients (2.5±0.2) and CTL individuals (1.0±0.07). β-GP further increased calcification in respective groups (51.3±4.7; 13.5±1.1 and 9.8±1.2). Msx2 mRNA expression decreased in CTL SMC after β-GP (48h) and did not change in PAD without DM and in PAD+DM SMC. Msx2 protein expression decreased in all groups after calcifying medium (72h). Moreover, Runx2 mRNA expression increased in β-GP-treated PAD+DM SMC and ALPL mRNA expression augmented in β-GP-treated SMC from PAD without DM patients. However, Runx2 and ALPL protein expression was not modulated by calcifying medium (72h) in all groups. Interestingly, immunofluorescence of arterial samples demonstrated increased osteochondrogenic protein expression (Msx2, Runx2 and ALPL) around calcifying foci in PAD+DM group (+++) versus PAD without DM (++) and versus CTL (+) patients. Coincidently, PAD+DM group showed augmented vascular calcification (19x10 4 ±10x10 4 ; 7x10 4 ±2x10 4 ; 3x10 3 ±10 -2 μm 2 ). Conclusion: We demonstrated increased calcification in arteries from amputated PAD+DM patients and in respective SMC without and with calcifying medium. Although we found augmented osteochondrogenic protein expression in the vessel wall from PAD+DM individuals this was not robustly shown in isolated SMC in vitro .

MicroRNA-153-3p Inhibits Cell Proliferation and Increases Apoptosis in Human Vascular Smooth Muscle Cells in Peripheral Arterial Disease

2020 ◽  
Vol 10 (5) ◽  
pp. 632-639
Author(s):  
Ran Lu ◽  
Xiaohu Yang
Keyword(s):  
Smooth Muscle ◽  
Peripheral Arterial Disease ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Viability ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Arterial Disease ◽  
And Migration ◽  
Peripheral Arterial

Objective: This study explored the role and molecular mechanism of microRNA-153-3p (miR-1533p) in human vascular smooth muscle cells in peripheral arterial disease. Methods: Human vascular smooth muscle cells (hVSMCs) were transfected with mimic control, miR-153-3p mimic, inhibitor control or miR-153-3p inhibitor for 48 h, and qRT-PCR was applied to determine the transfection efficiency. Meanwhile, the cell viability, cell migration and apoptosis of the transfected cells were measured via MTT assay, flow cytometry analysis and Transwell assay respectively. The target gene of miR-153-3p was predicted by TargetScan and verified by the dual luciferase reporter gene system. In addition, mimic control, miR-153-3p mimic, miR-153-3p mimic + control-plasmid, or miR-153-3p mimic+BCL2-plasmid was transfected into hVSMCs for 48 h, and the cell viability, migration and apoptosis were determined. Besides, qRT-PCR and Western blot assay were applied to measure the expression of BCL2 at both mRNA and protein levels in hVSMCs after the transfection. Results: Compared with the control group, miR-153-3p mimic improved the expression of miR-153-3p, and miR-153-3p inhibitor down-regulated miR-153-3p in hVSMCs. miR-153-3p mimic could reduce the cell viability and migration, and induce the cell apoptosis of hVSMCs. However, miR-153-3p inhibitor had the opposite effects on the cell viability, migration and apoptosis on hVSMCs. We predicted and verified that BCL2 was a direct target of miR-153-3p. In addition, all the effects of miR-153-3p mimic on hVSMCs were significantly reversed by BCL2-plasmid. Conclusion: We demonstrated that miR-153-3p participated in the development and progression of peripheral arterial disease through regulating the proliferation and migration of vascular smooth muscle cells by targeting BCL2.

Fifteen Coagulation and Fibrinolysis Parameters in Diabetes Mellitus and in Patients with Vasculopathy

1984 ◽  
Vol 52 (02) ◽  
pp. 138-143 ◽  
Author(s):  
M Christe ◽  
J Fritschi ◽  
B Lämmle ◽  
T H Tran ◽  
G A Marbet ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Peripheral Arterial Disease ◽  
Negative Correlation ◽  
Fibrinolytic Activity ◽  
Antithrombin Iii ◽  
Predictor Variable ◽  
Arterial Disease ◽  
Thrombin Time ◽  
Peripheral Arterial ◽  
Coagulation And Fibrinolysis

SummaryFifteen haemostasis parameters have been measured in 48 normal persons, 36 diabetics without and 44 with complications and 27 with peripheral arterial disease. Since the patients groups are older than normals, part of the differences are due to age. However, the differences are significant between normals and patients. They become highly significant for the diabetics with complications and nephropathy (Table 7). In diabetics without complications factor VIII functions, fibrinogen and thrombin time are related to age whereas there is a negative correlation for the fibrinolytic activity and antithrombin III. The diabetic complications shade off the correlations, which subsist only for VIIIR: CoF, VIIIR: Ag, ATIII and lysis before stasis. With Hbalc as dependent variable VIIIR:CoF is the only significant predictor variable in diabetics (Table 9).

scholarly journals The relation of anatomical distribution of symptomatic peripheral arterial disease (PAD) with HbA1c level in patients with type 2 diabetes mellitus

2021 ◽  
Vol 12 ◽  
pp. 204201882110005
Author(s):  
Nawaf J. Shatnawi ◽  
Nabil A. Al-Zoubi ◽  
Hassan M. Hawamdeh ◽  
Yousef S. Khader ◽  
Mowafeq Heis ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Peripheral Arterial Disease ◽  
Hba1c Level ◽  
Arterial Disease ◽  
Anatomical Distribution ◽  
The Mean ◽  
Peripheral Arterial

Aims: Increased level of glycated hemoglobin (HbA1c) is associated with an increased prevalence of peripheral arterial disease (PAD). This study aimed to assess the relationship between the anatomical distribution of symptomatic PAD lesions in patients with type 2 diabetes and HbA1c levels at the time of PAD diagnosis. Patients and methods: A retrospective study was conducted at King Abdullah University Hospital during the period August 2011 to December 2015. Consecutive patients with type 2 diabetes presented with symptomatic PAD confirmed by computed tomography-angiography (CTA) were included in this study. CTA images were reviewed. Relevant information including demographic data, PAD symptoms, comorbidities, HbA1c level, lipid profile, C-reactive protein and the mean platelets volume were retrieved from medical records. Results: A total of 332 patients with type 2 diabetes (255 males and 77 females) were included in this study. The mean HbA1c at the time of PAD diagnosis was 8.68% (±2.06%). The prevalence of hemodynamic relevant atherosclerotic lesions of the superficial femoral artery, popliteal artery, leg vessels, femoro-popliteal, and crural segments was significantly higher in patients with HbA1c >7.5% compared with patients with HbA1c ⩽7.5%. Conclusion: The anatomical distribution of symptomatic PAD in patients with type 2 diabetes mellitus differed significantly according to HbA1c level at the time of PAD diagnosis.

scholarly journals MicroRNA-223-3p inhibits vascular calcification and the osteogenic switch of vascular smooth muscle cells

2021 ◽  
Vol 296 ◽  
pp. 100483
Author(s):  
Yingchun Han ◽  
Jichao Zhang ◽  
Shan Huang ◽  
Naixuan Cheng ◽  
Congcong Zhang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells
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