Abstract P198: WNK-SPAK-NKCC1 Cascade Activation Contributes to Worsened Brain Damage in Mice With Hypertension Co-Morbidity after Ischemic Stroke

AbstractPhotochemical thrombosis is a method for the induction of ischemic stroke in the cerebral cortex. It can generate localized ischemic infarcts in the desired region; therefore, it has been actively employed in establishing an ischemic stroke animal model and in vivo assays of diagnostic and therapeutic techniques for stroke. To establish a rabbit ischemic stroke model and overcome the shortcoming of previous studies that were difficult to build a standardized photothrombotic rabbit model, we developed a photochemical thrombosis induction system that can produce consistent brain damage on a specific area. To verify the generation of photothrombotic brain damage using the system, longitudinal magnetic resonance imaging, 2,3,5-triphenyltetrazolium chloride staining, and histological staining were applied. These analytical methods have a high correlation for ischemic infarction and are appropriate for analyzing photothrombotic brain damage in the rabbit brain. The results indicated that the photothrombosis induction system has a main advantage of being accurately controlled a targeted region of photothrombosis and can produce cerebral hemisphere lesions on the target region of the rabbit brain. In conjugation with brain atlas, it can induce photochemical ischemic stroke locally in the part of the brain that is responsible for a particular brain function and the system can be used to develop animal models with degraded specific functions. Also, the photochemical thrombosis induction system and a standardized rabbit ischemic stroke model that uses this system have the potential to be used for verifications of biomedical techniques for ischemic stroke at a preclinical stage in parallel with further performance improvements.

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Inhibition of sonic hedgehog signaling aggravates brain damage associated with the down-regulation of Gli1, Ptch1 and SOD1 expression in acute ischemic stroke

Neuroscience Letters ◽

10.1016/j.neulet.2011.11.027 ◽

2012 ◽

Vol 506 (1) ◽

pp. 1-6 ◽

Cited By ~ 35

Author(s):

Hui Ji ◽

Jiangyong Miao ◽

Xiangjian Zhang ◽

Yuanyuan Du ◽

Haichao Liu ◽

...

Keyword(s):

Ischemic Stroke ◽

Acute Ischemic Stroke ◽

Brain Damage ◽

Sonic Hedgehog ◽

Hedgehog Signaling ◽

Down Regulation ◽

Sonic Hedgehog Signaling

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Aging aggravates ischemic stroke-induced brain damage in mice with chronic peripheral infection

Aging Cell ◽

10.1111/acel.12106 ◽

2013 ◽

Vol 12 (5) ◽

pp. 842-850 ◽

Cited By ~ 22

Author(s):

Hiramani Dhungana ◽

Tarja Malm ◽

Adam Denes ◽

Piia Valonen ◽

Sara Wojciechowski ◽

...

Keyword(s):

Ischemic Stroke ◽

Brain Damage ◽

Peripheral Infection

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Early administration of MPC-n(IVIg) selectively accumulates in ischemic areas to protect inflammation-induced brain damage from ischemic stroke

Theranostics ◽

10.7150/thno.58947 ◽

2021 ◽

Vol 11 (17) ◽

pp. 8197-8217

Author(s):

Weili Jin ◽

Ye Wu ◽

Ning Chen ◽

Qixue Wang ◽

Yunfei Wang ◽

...

Keyword(s):

Ischemic Stroke ◽

Brain Damage ◽

Early Administration

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Supplemental Oxygen Use in Ischemic Stroke Patients, Does Utilization Correspond to Need for Oxygen Therapy?

Stroke ◽

10.1161/str.32.suppl_1.382-b ◽

2001 ◽

Vol 32 (suppl_1) ◽

pp. 382-382

Author(s):

Arthur M Pancioli ◽

Mark J Bullard ◽

Mary E Grulee ◽

Edward C Jauch ◽

David F Perkis

Keyword(s):

Ischemic Stroke ◽

Oxygen Therapy ◽

Supplemental Oxygen ◽

University Hospital ◽

Extensive Literature ◽

Oxygen Utilization ◽

Stroke Patients ◽

Co Morbidity ◽

History Of ◽

Explicit Criteria

P236 Background: In 1994, the American Heart Association Stroke Council stated that there is no data to support routine use of supplemental oxygen in stroke patients. The purpose of this study was to determine the degree of supplemental oxygen use in ischemic stroke and whether patients receiving oxygen met criteria for therapy. Methods: An extensive literature search was performed to generate a comprehensive list of explicit criteria for supplemental oxygen use. When the literature disagreed, criteria were included in the list to overestimate rather than underestimate the justification for oxygen use. A retrospective chart review of 169 consecutive, non-intubated, ischemic stroke patients admitted to a university hospital during calendar year 1998 was performed. Two patients were excluded due to missing data yielding a sample of 167 patients. Data included demographic and co-morbidity information as well as a check of each inpatient day for documentation of any of the explicit criteria for supplemental oxygen use. Results: A total of 167 patient charts were reviewed yielding a total of 600 inpatient days abstracted. Of the 167 patients, 102 (61.1%) received oxygen accounting for 322 patient days. Of the 322 inpatient days that patients received oxygen, 147 (45.6%) met at least one criteria for oxygen utilization. Of the 278 inpatient days that patients did not receive oxygen 69 (24.8%) would have met at least one of the criteria for oxygen use. For the entire population, statistically significant factors associated with patients receiving oxygen included: presence of at least one justifying criteria, increasing age, white race, male gender, history of atrial fibrillation, and a history of hypertension. Conclusion: We developed a comprehensive, literature-based list of criteria for supplemental oxygen therapy that was designed to overestimate rather than underestimate the justification of oxygen utilization. Using this explicit list, only 45.6% of days of oxygen therapy were justified in our ischemic stroke population. This study demonstrates that oxygen therapy is commonly given to ischemic stroke victims without clear indication.

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Abstract T P21: Improved Ischemic Stroke Outcomes After Intra-Arterial Interventions Associated With Higher HDL Cholesterol

Stroke ◽

10.1161/str.45.suppl_1.tp21 ◽

2014 ◽

Vol 45 (suppl_1) ◽

Author(s):

Ali Sultan-Qurraie ◽

Adam de Havenon ◽

David L Tirschwell ◽

Deepak Sharma ◽

Basavaraj Ghodke ◽

...

Keyword(s):

Ischemic Stroke ◽

Linear Models ◽

Univariate Analysis ◽

Hdl Cholesterol ◽

Continuous Variable ◽

Favorable Outcome ◽

Stroke Risk Factor ◽

Co Morbidity ◽

History Of ◽

Relationship Of

Background/Objective: The effect of pre-morbid lipid levels on neurologic outcome after Acute Ischemic Stroke (AIS) is variable in the published literature. In this study, we retrospectively use a cohort to evaluate the relationship of cholesterol subfractions to outcome. Methods: The cohort consists of AIS patients treated with IA therapy between September 2008 and December 2010. Favorable outcome for these analyses was defined as a modified Rankin Scale of ≤ 3 at time of discharge. Univariate associations with favorable outcome were sought for demographic, co-morbidity, stroke risk factor, and procedure related variables. Independent associations were identified by relative risk regression using generalized linear models with a logit link, a Poisson distribution for variance and robust standard errors. All analyses were performed in STATA. Results: The cohort included 42 patients; mean age was 62 years, 62% were women, median pre-procedure NIHSS was 16, 70% of patients achieved a mRS ≤ 3. Univariate analysis suggested significance of a number of variables with favorable outcome. In multivariate analysis only three variables-previous tobacco use, highest procedural SBP, and history of atrial fibrillation-remained significant. Adding LDL to the multivariate model did not show association, though adding HDL as a continuous variable did show an association. Results are shown in the table. Conclusions: In our cohort there was no association between favorable outcome at time of discharge and LDL, but higher HDL was associated with an increased chance of good outcome. While this association between HDL and outcome is reported for patients treated with intravenous tPA, it is novel for patients treated with IA therapy and warrants further study. Our data collection is ongoing and we aim to double the size of our dataset; the updated analyses will be presented at the meeting.

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Abstract TP383: Ischemic Stroke Patients With Diabetes Looking for Practices in Need of Improvement

Stroke ◽

10.1161/str.51.suppl_1.tp383 ◽

2020 ◽

Vol 51 (Suppl_1) ◽

Author(s):

Cynthia Bautista ◽

Sally Gerard

Keyword(s):

Ischemic Stroke ◽

Cause Of Death ◽

Demographic Data ◽

Stroke Care ◽

The United States ◽

Stroke Patients ◽

Co Morbidity ◽

Patients With Diabetes ◽

Care Outcomes ◽

Stroke Center

Background/Purpose: Stroke is the fifth leading cause of death and Diabetes is the seventh leading cause of death in the United States. Diabetes is an independent risk factor for stroke. Diabetes is a common co-morbidity in stroke patients and is associated with poor outcomes after stroke. Get with the Guidelines - Stroke (GWTG-S) Registry database provides a rich opportunity to look at disease-specific data and find opportunities for improving care. The purpose of this study was to examine specific elements of acute ischemic stroke care in patients with diabetes using the GWTG-S at Comprehensive and Primary Stroke Centers in Northeast of America. Methods: A retrospective, descriptive study at both a Comprehensive and Primary Stroke Center. The analysis focused on patients with ischemic stroke and diabetes entered in the GWTG-S from January 1, 2015, to December 31, 2017. Data were analyzed looking at measures specific to stroke and the presence of diabetes. General demographic data were examined to compare populations and quality outcome measures. Results: The sample of patients with ischemic stroke and diabetes was over 1,000 patient’s at the two sites (Comprehensive site N = 804, Primary site N = 203) Incidence of ischemic stroke with diabetes at the two sites were 32% and 26%, respectively. Demographic data were similar in most categories including age, race, and gender. Significant differences were found in regard to the type of insurance. Stroke care outcomes indicated thrombolytic administration rates were the same at 8%. Diabetes care outcomes indicated patients discharged on insulin occurred in 18% to 26% of the sample. Conclusion/Implications for Practice: Ischemic stroke patients with diabetes were shown to receive similar care at both a comprehensive and primary care stroke center. There were no differences between centers in thrombolysis treatment for ischemic stroke patients with diabetes. Several opportunities for improvement in diabetes-related care need to be addressed.

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