Bradynema listronoti n. sp. (Nematoda: Allantonematidae), a parasite of the carrot weevil Listronotus oregonensis (Coleoptera: Curculionidae) in Quebec, Canada

Nematology ◽  
2007 ◽  
Vol 9 (5) ◽  
pp. 608-622 ◽  
Author(s):  
Kelley Thomas ◽  
Guy Boivin ◽  
Guy Bélair ◽  
Robin Giblin-Davis ◽  
Yongsan Zeng ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Molecular Analysis ◽  
New Genus ◽  
Large Subunit ◽  
Ventral Side ◽  
The Other ◽  
Lsu Rdna ◽  
Listronotus Oregonensis ◽  
Carrot Weevil ◽  
Shared Characteristic

AbstractBradynema listronoti n. sp. was collected from the haemocoel of adults and the environment of the carrot weevil, Listronotus oregonensis in Quebec, Canada. It is the first species of Bradynema to be reported from weevils and is described and illustrated herein. Bradynema listronoti n. sp. appears to be closest to B. trixagi because of the shared characteristic of a peloderan bursa in males which is not present in the other seven described species in the genus. Bradynema listronoti n. sp. can be differentiated from all members of the genus by the possession of a degenerate, but sometimes visible, stylet remnant, non-degenerate pharynx in males and by the often dorsally-curved C-shaped body (ventral side is convex) of parasitic females. The vestigial stylet in all stages argues for placement of B. listronoti n. sp. within the genus Bradynema, but some characteristics, such as a non-degenerate pharynx in males, suggest placement in a new genus or affinities with other genera in the Allantonematidae, e.g., Allantonema. Molecular analysis of the near full length SSU, D2/D3 expansion segments of the large subunit (LSU) rDNA and partial mitochondrial DNA COI (mtCOI) suggest that this nematode is unique and D2/D3 analysis supports monophyly with B. rigidum, the only other member of the genus to be sequenced.

Molecular variability of Schistonchus caprifici (Nematoda: Aphelenchoididae) from Ficus carica in Turkey

Nematology ◽  
2008 ◽  
Vol 10 (5) ◽  
pp. 639-649 ◽  
Author(s):  
Baris Gulcu ◽  
Selçuk Hazir ◽  
Robin M. Giblin-Davis ◽  
Weimin Ye ◽  
Natsumi Kanzaki ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Intraspecific Variation ◽  
Human Activities ◽  
Large Subunit ◽  
Ficus Carica ◽  
Lsu Rdna ◽  
Molecular Analyses ◽  
Molecular Variability ◽  
Geographic Patterns ◽  
Fig Trees

Abstract An extensive biogeographical survey was conducted from 2004 through part of 2007 of nematodes associated with the edible fig, Ficus carica, in seven different regions of Turkey. Figs (syconia) were collected, dissected and harvested for nematodes from 308 apparently wild or naturalised F. carica trees along roadsides and trails. Nematode specimens were examined morphologically and molecular analyses of sequences of the D2/D3 expansion segments of the large subunit (LSU) rDNA and partial mitochondrial DNA COI (mtCOI) were done. Of the 308 fig trees, 150 (49%) were positive for what was morphologically confirmed to be Schistonchus caprifici. Figs on one tree in Kahramanmaras city, South East Turkey, were found to contain S. caprifici and dauer juveniles of what appears to be a diplogastrid nematode. Molecular analyses produced congruent trees for both sequenced loci supporting the hypothesis that S. caprifici from different locations in Turkey are part of a well supported clade with some inherent variability. No geographic patterns were observed for either locus suggesting that the sequences generated represent inherent intraspecific variation for S. caprifici and that human activities related to the culture of the edible fig have allowed for mixing of any previously allopatric lineages.

Taxonomic aspects of Peronosporaceae inferred from Bayesian molecular phylogenetics

2003 ◽  
Vol 81 (7) ◽  
pp. 672-683 ◽  
Author(s):  
M Göker ◽  
H Voglmayr ◽  
A Riethmüller ◽  
M Weiß ◽  
F Oberwinkler
Keyword(s):  
Phylogenetic Analysis ◽  
Representative Sample ◽  
Molecular Phylogenetics ◽  
New Genus ◽  
28S Rdna ◽  
The Other ◽  
Morphological Evidence ◽  
Lsu Rdna ◽  
Bayesian Phylogenetic Analysis ◽  
Downy Mildews

We present the results of a Bayesian phylogenetic analysis of parts of the nuclear 28S rDNA of a representative sample of the Peronosporales. Peronospora s.l. is shown to be paraphyletic. Based on molecular and morphological evidence, several species of the genus Peronospora are transferred to Hyaloperonospora. Plasmopara oplismeni appears to be related only distantly to the other Plasmopara species, and is transferred to the new genus Viennotia based on molecular, morphological, and ecological evidence. The remaining Plasmopara species are likely to be paraphyletic with respect to Bremia, Paraperonospora, and Basidiophora. Phytophthora is shown to be paraphyletic with respect to the obligatory biotrophic genera. Evidence for the assumption that obligatory biotrophism arose independently at least twice in Peronosporales is demonstrated.Key words: LSU rDNA, Straminipila, Peronosporomycetes, Peronosporales, downy mildews, Bayesian phylogenetic analysis.

Varialvus gen. nov. (Digenea, Cryptogonimidae), from species of Lutjanidae (Perciformes) off the Great Barrier Reef, New Caledonia and the Maldives

2010 ◽  
Vol 55 (4) ◽  
Author(s):  
Terrence Miller ◽  
Rodney Bray ◽  
Jean-Lou Justine ◽  
Thomas Cribb
Keyword(s):  
Great Barrier Reef ◽  
New Genus ◽  
New Caledonia ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Lsu Rdna ◽  
Barrier Reef ◽  
Host Group ◽  
West Pacific

AbstractA survey of the cryptogonimid trematode fauna infecting Indo-West Pacific Lutjanidae (Perciformes) revealed the presence of four new species whose morphological and genetic differences relative to all other known cryptogonimids warrants the proposal of a new genus, Varialvus gen. nov. Species of this new genus were recovered from sites off Heron and Lizard Islands on the Great Barrier Reef, Australia, New Caledonia and Rasdhoo Atoll, Maldives. Varialvus gen. nov. is distinguished from all other cryptogonimid genera by the combination of a fusiform to oval body, the relatively small number of large oral spines, a median ovary which is relatively condensed and highly lobed, opposite to slightly oblique testes, uterine loops that are restricted to the hindbody and extend well posterior to the testes, and vitelline follicles that are mainly in the forebody but may extend from the anterior margin of the ovary to anterior to the intestinal bifurcation. Bayesian inference analysis of partial large subunit (LSU) rDNA sequence data for these species revealed that they formed a monophyletic clade, despite V. charadrus sp. nov. having a distinctly muscular gonotyl, which based on morphological characters alone may have warranted placement in a separate genus in the absence of DNA sequence data. At least one species of Varialvus gen. nov. is apparently widespread in the Indo-West Pacific. Three species, V. lacertus sp. nov., V. jenae sp. nov. and V. angustus sp. nov. have each been found at only one locality, but V. charadrus sp. nov. was recovered from lutjanids off the Great Barrier Reef, New Caledonia and the Maldives, demonstrating a biogeographic range of at least 10,000 kilometres. Siphoderina lutjani (Saoud, Ramadan et Al Kawari, 1988) Miller et Cribb, 2008 is transferred here as V. lutjani (Saoud, Ramadan et Al Kawari, 1988) n. comb. based on morphological and host group agreement with species of Varialvus gen. nov.

scholarly journals Four New Keratinophyton Species (Onygenaceae) From Europe

2020 ◽  
Author(s):  
Roman Labuda ◽  
Andreas Bernreiter ◽  
Doris Hochenauer ◽  
Christoph Schüller ◽  
Alena Kubatova
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Its Region ◽  
Soil Samples ◽  
The Other ◽  
Lsu Rdna ◽  
New Combinations ◽  
Internal Transcribed Spacer Region ◽  
Asexual Morphs ◽  
Terminal Cluster

Abstract Four new Keratinophyton species (Ascomycota: Pezizomycotina, Onygenales), K. gollerae, K. lemmensii, K. straussii and K. wagneri, isolated from soil samples originating from Europe (Austria, Italy and Slovakia) are described and illustrated. The new taxa are well supported by phylogenetic analysis of the internal transcribed spacer region (ITS) region, the nuclear large subunit (LSU) rDNA, and their phenotype. Within the Keratinophyton clade, K. lemmensii is clustered with K. durum, K. hubeiense, K. submersum and K. siglerae, while K. gollerae, K. straussii and K. wagneri are resolved in a separate terminal cluster along with K. minutisporosum. All four new species can be well distinguished from the other asexual taxa in the genus Keratinophyton based on phenotypical characteristics alone. Ten new combinations are proposed for all other Chrysosporium asexual morphs which are resolved in the monophyletic Keratinophyton clade.

Phylogenetic studies on nuclear large subunit ribosomal DNA sequences of smut fungi and related taxa

1997 ◽  
Vol 75 (12) ◽  
pp. 2045-2056 ◽  
Author(s):  
Dominik Begerow ◽  
Robert Bauer ◽  
Franz Oberwinkler
Keyword(s):  
Molecular Phylogeny ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Large Subunit ◽  
The Other ◽  
Lsu Rdna ◽  
Smut Fungi ◽  
Neighbor Joining ◽  
Phylogenetic Studies ◽  
Key Words Basidiomycete

To show phylogenetic relationships among the smut fungi and their relatives, we sequenced a part of the nuclear LSU rDNA from 43 different species of smut fungi and related taxa. Our data were combined with the existing sequences of seven further smut fungi and 17 other basidiomycetes. Two sets of sequences were analyzed. The first set with a representative number of simple septate basidiomycetes, complex septate basidiomycetes, and smut fungi was analyzed with the neighbor-joining method to estimate the general topology of the basidiomycetes phylogeny and the positions of the smut fungi. The tripartite subclassification of the basidiomycetes into the Urediniomycetes, Ustilaginomycetes, and Hymenomycetes was confirmed and two groups of smut fungi appeared. The smut genera Aurantiosporium, Microbotryum, Fulvisporium, and Ustilentyloma are members of the Urediniomycetes, whereas the other smut species tested are members of the Ustilaginomycetes with Entorrhiza as a basal taxon. The second set of 46 Ustilaginomycetes was analyzed using the neighbor-joining and the maximum parsimony methods to show the inner topology of the Ustilaginomycetes. The results indicated three major lineages among Ustilaginomycetes corresponding to the Entorrhizomycetidae, Exobasidiomycetidae, and Ustilaginomycetidae. The Entorrhizomycetidae are represented by Entorrhiza species. The Ustilaginomycetidae contain at least two groups, the Urocystales and Ustilaginales. The Exobasidiomycetidae include five orders, i.e., Doassansiales, Entylomatales, Exobasidiales, Georgefischeriales, and Tilletiales, and Graphiola phoenicis and Microstroma juglandis. Our results support a classification mainly based on ultrastructure. The description of the Glomosporiaceae is emended. The Doassansiopsaceae, Melanotaeniaceae, and Urocystaceae are proposed as new taxa. Key words: basidiomycete systematics, LSU rDNA, Microbotryales, molecular phylogeny, smut fungi, Ustilaginomycetes.

scholarly journals Cryptococcus rajasthanensis sp. nov., an anamorphic yeast species related to Cryptococcus laurentii, isolated from Rajasthan, India

2007 ◽  
Vol 57 (2) ◽  
pp. 414-418 ◽  
Author(s):  
Puja Saluja ◽  
G. S. Prasad
Keyword(s):  
Large Subunit ◽  
Its Region ◽  
Molecular Data ◽  
Lsu Rdna ◽  
Cryptococcus Laurentii ◽  
The Novel ◽  
D2 Domain ◽  
Its Regions ◽  
Anamorphic Yeast ◽  
Physiological Tests

Two novel anamorphic yeast strains (S-15LT and 3-C1) were isolated from the inflorescences of plants collected in two different towns in Rajasthan State, India. Sequencing of the D1/D2 domains of the large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) regions suggested they are strains of the same species. Phenotypic characteristics such as the absence of fermentation, the absence of sexual structures and ballistoconidia, the assimilation of myo-inositol and d-glucuronate, and positive Diazonium blue B and urease reactions indicated that these strains belong to the genus Cryptococcus. The novel strains differed from Cryptococcus laurentii in six physiological tests and differed from other related species in more than six tests. A phylogenetic analysis of the sequences of the D1/D2 domains of the LSU rDNA and the ITS regions placed these strains in the Bulleromyces clade within the order Tremellales, with C. laurentii as their closest described relative. The novel strains showed 1.6 and 7.5 % divergence in the D1/D2 domain of the LSU rDNA and ITS regions, respectively, with respect to C. laurentii. The divergence from other species was more than 3 % for the D1/D2 domain and more than 9 % for the ITS region. On the basis of the phenotypic and molecular data, strains S-15LT and 3-C1 represent a novel species within the genus Cryptococcus, for which the name Cryptococcus rajasthanensis sp. nov. is proposed. The type strain is S-15LT (=MTCC 7075T=CBS 10406T).

scholarly journals Discovery of the Smallest Lace Bug from Mid-Cretaceous of Northern Myanmar Supports the Hypothesis of a Miniaturization Phenomenon of Insects in Kachin Amber (Hemiptera: Heteroptera: Tingidae)

Taxonomy ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 60-68
Author(s):  
Jun Souma ◽  
Shûhei Yamamoto ◽  
Yui Takahashi
Keyword(s):  
New Species ◽  
New Genus ◽  
The Other ◽  
Diagnostic Characters ◽  
Lace Bug ◽  
New Finding ◽  
A New Species

A total of 14 species in seven tingid genera have been described from the mid-Cretaceous Burmese (Kachin) amber from northern Myanmar, with very distinct paleofauna. Here, a new species of a new genus, Burmavianaida anomalocapitata gen. et sp. nov., is described from Kachin amber. This new species can be readily distinguished from the other described tingid taxa by the apparently smaller body and the structures of the pronotum and hemelytron. Burmavianaida gen. nov. shares the diagnostic characters with two clades composed of three extant subfamilies (Cantacaderinae + Tinginae) and Vianaidinae and may represent an extinct clade distinct from them. To the best of our knowledge, B. anomalocapitata sp. nov. is the smallest species of Tingidae among over 2600 described species. Our new finding supports the hypothesis of the miniaturization phenomenon of insects in Kachin amber, as suggested by previous studies.

The Leptobrachium (Anura: Megophryidae) of the Langbian Plateau, southern Vietnam, with description of a new species

Zootaxa ◽  
2011 ◽  
Vol 2804 (1) ◽  
pp. 25 ◽  
Author(s):  
BRYAN L. STUART ◽  
JODI J. L. ROWLEY ◽  
DAO THI ANH TRAN ◽  
DUONG THI THUY LE ◽  
HUY DUC HOANG
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Small Body ◽  
Original Description ◽  
The Other ◽  
Sexually Active ◽  
Small Body Size ◽  
Upper Lip ◽  
Southern Vietnam ◽  
A New Species

We sampled two forms of Leptobrachium in syntopy at the type locality of L. pullum at upper elevations on the Langbian Plateau, southern Vietnam. The two forms differed in morphology (primarily in coloration), mitochondrial DNA, and male advertisement calls. One form closely agrees with the type series of L. pullum (but not to its original description due to error), and the other is described as new. Leptobrachium leucops sp. nov. is distinguished from its congeners by having small body size (males with SVL 38.8–45.2), the upper one-third to one-half of iris white, a blue scleral arc, a dark venter, and sexually active males without spines on the upper lip. Leptobrachium pullum and L. mouhoti, a recently described species from low-elevation slopes of the Langbian Plateau in eastern Cambodia, are morphologically divergent but genetically similar, warranting further investigation into geographic variation in the red-eyed Leptobrachium of southern Indochina.

Morphometrics and molecular analysis ofOzolaimus linstowin. sp. (Oxyuroidea: Pharyngodonidae) from the green lizardIguana iguana

2015 ◽  
Vol 90 (2) ◽  
pp. 186-198 ◽  
Author(s):  
S.V. Malysheva
Keyword(s):  
Body Size ◽  
Large Subunit ◽  
Buccal Capsule ◽  
Small Subunit ◽  
Present Species ◽  
Lsu Rdna ◽  
Adult Males ◽  
Spicule Length ◽  
Males And Females ◽  
Characteristic Features

AbstractOzolaimus linstowin. sp. is described from the large intestine ofIguana iguanaLinnaeus, 1758 from Mexico. The present species can be easily distinguished fromO. megatyphlonandO. cirratusby the presence of a long and slender pharynx not divided into sections, more similar to the remaining two species,O. monhysteraandO. ctenosauri. Ozolaimus linstowin. sp. can be differentiated fromO. monhysteraby the shorter spicule length and smaller body size of both males and females. Males ofO. linstowin. sp. are morphologically close to those ofO. ctenosauri, but females possess a markedly smaller body size and differ in the organization of the oral cuticular armature. Adult males ofO. linstowin. sp. bear some characteristic features of the J3 juvenile morphology in terms of the cuticular organization of the oral and buccal capsule. Phylogenetic analysis ofO.linstowin. sp. using partial small subunit (SSU) and D2–D3 large subunit (LSU) rDNA shows relationships with several Oxyuridae genera.

Affinities of the Boletus chromapes group to Royoungia and the description of two new genera, Harrya and Australopilus

2012 ◽  
Vol 25 (6) ◽  
pp. 418 ◽  
Author(s):  
Roy E. Halling ◽  
Mitchell Nuhn ◽  
Todd Osmundson ◽  
Nigel Fechner ◽  
James M. Trappe ◽  
...  
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Sister Group ◽  
Molecular Data ◽  
Translation Elongation ◽  
Spore Deposit ◽  
Large Subunit Rdna ◽  
Translation Elongation Factor 1Α

Harrya is described as a new genus of Boletaceae to accommodate Boletus chromapes, a pink-capped bolete with a finely scabrous stipe adorned with pink scabers, a chrome yellow base and a reddish-brown spore deposit. Phylogenetic analyses of large-subunit rDNA and translation elongation factor 1α confirmed Harrya as a unique generic lineage with two species, one of which is newly described (H. atriceps). Some Chinese taxa were recently placed in a separate genus, Zangia, supported by both morphology and molecular data. Multiple accessions from Queensland, Australia, support the synonymy of at least three species in a separate Australian clade in the new genus, Australopilus. The truffle-like Royoungia is also supported as a separate lineage in this clade of boletes. Even though it lacks stipe characters, it possesses the deep, bright yellow to orange pigments in the peridium. Additional collections from Zambia and Thailand represent independent lineages of uncertain phylogenetic placement in the Chromapes complex, but sampling is insufficient for formal description of new species. Specimens from Java referable to Tylopilus pernanus appear to be a sister group of the Harrya lineage.

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